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Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis

由在少年的致病的 miR-21 的 JAK/STAT 信号小径的规定自发的关节炎

作     者:Hong-Wei Li Hua-Song Zeng Hong-Wei Li;Hua-Song Zeng

作者机构:Department of Pediatric Allergy Immunology and RheumatologyGuangzhou Women and Children Medical CenterGuangzhou 510623China Department of Pediatric AllergyImmunology and RheumatologyGuangzhou Women and Children's Medical CenterGuangzhou Medical University9 Jinsui RoadGuangzhou 510175China 

出 版 物:《World Journal of Pediatrics》 (世界儿科杂志(英文版))

年 卷 期:2020年第16卷第5期

页      面:502-513页

核心收录:

学科分类:1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基  金:This study was supported by Natural Science Foundation of Guangdong Province(No.2017A030313557) Guangzhou Health Bureau(No.20171A011256) Guangzhou Municipal Science and Technology Project(No.201607010316) 

主  题:JAK/STAT Juvenile idiopathic arthritis MiR-21 Signal transducer and activator of transcription 3 (STAT3)/p-STAT3 

摘      要:Background Overexpression of the components of the Janus kinase/signal transducer and activator of transcription(JAK/STAT)signalling pathway is the key factor of the pathogenic mechanisms underlying systemic juvenile idiopathic arthritis(sJIA).The study aims to investigate the association between miR-21 and the JAK/STAT signal pathway in *** Total RNA was extracted from peripheral blood mononuclear cells(PBMCs)in active JIA *** relative expressions of miR-21,STAT3 and suppressor of cytokine signalling 3 in PBMCs were measured by real-time polymerase chain reaction and their expressions were measured by western blotting and dual-luciferase reported *** arthritis fibroblast-like synovial cell(RASF)was stimulated to become to osteoclasts using macrophage colony-stimulating factor(M-CSF)and factors that can impact on their differentiation ability were identified through the transfection of *** expression of STAT3/p-STAT3 was measured by western blot,and the levels of interleukin(IL)-17A,p65,matrix metalloproteinases(MMP)-3,MMP-4 and receptor activator of nuclear factor-κd3 after the LV3-miR-21 transfection were tested by enzyme-linked immunosorbent ***,the miR-21 targeted STAT3 gene was detected by the dualluciferase reported *** The expression of miR-21 was significantly lower in JIA patients than in healthy control(P0.05).The level of STAT3 was increased in PBMCs of JIA group compared with control group(P0.05).Furthermore,the expression levels of miR-21 in sJIA and polyarticular JIA groups were negatively correlated with STAT3(r=-0.5854/r=-0.6134,P0.05).The expression of STAT3 changed little in PBMCS after the stimulation of IL-6 and not in RASFs with transfection of *** expression of p-STAT3 decreased after the stimulation of IL-6 in RASFs transfected by LV3-miR-21(P0.05).RASFs were induced into osteoclasts using *** number of osteoclasts as determined by tartrate-resistant acid phospha

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