Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis

作     者：Hong-Wei Li Hua-Song Zeng Hong-Wei Li;Hua-Song Zeng

作者机构：Department of Pediatric Allergy Immunology and RheumatologyGuangzhou Women and Children Medical CenterGuangzhou 510623China Department of Pediatric AllergyImmunology and RheumatologyGuangzhou Women and Children's Medical CenterGuangzhou Medical University9 Jinsui RoadGuangzhou 510175China 

出 版 物：《World Journal of Pediatrics》 (世界儿科杂志（英文版）)

年 卷 期：2020年第16卷第5期

页      面：502-513页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基　　金：This study was supported by Natural Science Foundation of Guangdong Province(No.2017A030313557) Guangzhou Health Bureau(No.20171A011256) Guangzhou Municipal Science and Technology Project(No.201607010316) 

主　　题：JAK/STAT Juvenile idiopathic arthritis MiR-21 Signal transducer and activator of transcription 3 (STAT3)/p-STAT3 

摘      要：Background Overexpression of the components of the Janus kinase/signal transducer and activator of transcription(JAK/STAT)signalling pathway is the key factor of the pathogenic mechanisms underlying systemic juvenile idiopathic arthritis(sJIA).The study aims to investigate the association between miR-21 and the JAK/STAT signal pathway in *** Total RNA was extracted from peripheral blood mononuclear cells(PBMCs)in active JIA *** relative expressions of miR-21,STAT3 and suppressor of cytokine signalling 3 in PBMCs were measured by real-time polymerase chain reaction and their expressions were measured by western blotting and dual-luciferase reported *** arthritis fibroblast-like synovial cell(RASF)was stimulated to become to osteoclasts using macrophage colony-stimulating factor(M-CSF)and factors that can impact on their differentiation ability were identified through the transfection of *** expression of STAT3/p-STAT3 was measured by western blot,and the levels of interleukin(IL)-17A,p65,matrix metalloproteinases(MMP)-3,MMP-4 and receptor activator of nuclear factor-κd3 after the LV3-miR-21 transfection were tested by enzyme-linked immunosorbent ***,the miR-21 targeted STAT3 gene was detected by the dualluciferase reported *** The expression of miR-21 was significantly lower in JIA patients than in healthy control(P0.05).The level of STAT3 was increased in PBMCs of JIA group compared with control group(P0.05).Furthermore,the expression levels of miR-21 in sJIA and polyarticular JIA groups were negatively correlated with STAT3(r=-0.5854/r=-0.6134,P0.05).The expression of STAT3 changed little in PBMCS after the stimulation of IL-6 and not in RASFs with transfection of *** expression of p-STAT3 decreased after the stimulation of IL-6 in RASFs transfected by LV3-miR-21(P0.05).RASFs were induced into osteoclasts using *** number of osteoclasts as determined by tartrate-resistant acid phospha