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Emergency Biosafety Management Practice in Laboratory of Shelter Hospital

Current Medical Science 2020年第5期40卷 985-988页

作者： Yu-song LIU Duan-liang PENG jia YANG Dun-yan CHEN hong-bing jia Si-yuan YU Huan-huan CHEN Kang CHEN Lyu-rong LIU Sichuan National Emergency Medical Rescue Team/Sichuan Academy of Medical Sciences Sichuan Provincial People's Hospital(East)Chengdu 610100China Sichuan National Emergency Medical Rescue Team/Sichuan Academy of Medical Sciences Sichuan Provincial People's HospitalChengdu 610054China Fujian Provincial Hospital Fuzhou 350001China China-Japan Friendship Hospital Beijing 100029China Shanghai East Hospital Shanghai 200120China jiangsu Province Hospital/the First Afiliated Hospital of Nanjing Medical University Nanjing 210029China

At the end of 2019,the novel coronavirus infection outbroke in Wuhan,Hubei *** Feb.2,2020,Wuhan,as the worst-hit region,began to build“shelter hospital”rapidly to treat patients with mild *** shelter hospital has multiple functions such as emergency treatment,surgical treatment and clinical test,which can adapt to emergency medical rescue *** on the characteristics that shelter hospital only treats patients with mild ilness,tests of shelter laboratory,including coronavirus nucleic acid detection,IgM/IgG antibody serology detection,monitoring and auxiliary diagnosis and/or a required blood routine,urine routine,C-reactive protein,calcitonin original,biochemical indicators(liver enzymes,myocardial enzymes,renal function,etc.)and blood coagulation function test etc,were used to provide important basis for the diagnosis and treatment of the *** order to ensure laboratory biosafety,it is necessary to first evaluate the harm level of various *** the laboratory biosafety management,the harm level assessment of microorganisms is the core work of biosafety,which is of great significance to guarantee *** an emergency deployment afcted by the environment,shelter laboratory must possess strong *** paper will explore how to combine the biosafety model of traditional laboratory with the particularity of shelter laboratory to carry out effective work in response to the current epidemic.

关键词： COVID-19 shelter laboratory biosafety protection specimen processing

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Spread of Staphylococcus aureus between medical staff and high-frequency contact surfaces in a large metropolitan hospital

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International Journal of Nursing Sciences 2015年第4期2卷 366-370页

作者： Li-sha Shi Chun-juan Xu hong-bing jia Wei Chen Xiao-feng Zhou Xiu-hua Li School of Nursing Capital Medical UniversityBeijingChina China-Japan Friendship Hospital BeijingChina School of Nursing Beijing University of Chinese MedicineBeijingChina Chinese Nursing Association BeijingChina

Objective:To examine whether bacteria are transferred between the hands of medical staff and high-frequency contact surfaces within and between departments of a major metropolitan hospital,and to further analyze the patterns of ***:Microbiological samples were collected from the hands of 112 hospital employees as well as from 120 high-frequency contact surfaces in four hospital *** were collected on agar plates,analyzed for the presence of Staphylococcus aureus or methicillin-resistant ***(MRSA)by standard microbiology testing,and partially genotyped using pulsed-field gel ***:Genetically identical MRSA was identified on the surface of an electrocardiography device in the medical intensive care unit and on the same type of device in the neurosurgical *** similar *** was identified on an infusion pump in the medical intensive care unit and on the hands of several doctors in a different department who regularly use that *** identical *** was also identified on bedside rail restraint in the medical intensive care unit and on the hands of the nurse in the neurosurgical ***,genetically similar MRSA was identified both on the surface of an electrocardiography device and on the suction apparatus in the medical intensive care ***:Cross-contamination of *** or MRSA on medical workers'hands and contact surfaces was demonstrated within and between departments of a large metropolitan *** are needed in medical staff hygiene habits and in the cleaning of highfrequency contact surfaces to help prevent and control nosocomial infections.

关键词： Pathogenic bacteria Hand contamination Medical staff Nosocomial infection Cross-contamination

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Elevated Serum Ferritin is Associated with Insulin Resistance in Chinese Adults

Elevated Serum Ferritin is Associated with Insulin Resistanc...

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脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者： jiang Li hong-bing jia Hui Yang Shufa Du Berry M.Popkin Sheng-Kai Yan Department of Laboratory Medicine China-Japan Friendship HospitalMinistry of Health

Background:The health effects caused by iron overload have received increasing *** serum ferritin,a marker of iron stores,is associated with the prevalence and risk of insulin resistance,type 2 diabetes,cardiovascular disease,and metabolic *** observational studies have shown that elevated levels of circulating ferritin are associated with IR as assessed by the homeostatic model assessment(HOMA)and

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Toward Analytical Homogenized Relaxation Modulus for Fibrous Composite Material with Reduced Order Homogenization Method

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Computers, Materials & Continua 2025年第1期82卷 193-222页

作者： Huilin jia Shanqiao Huang Zifeng Yuan HEDPS Center for Applied Physics and Technology(CAPT)Department of Mechanics and Engineering SciencePeking UniversityBeijing100871China State Key Laboratory for Turbulence and Complex Systems Peking UniversityBeijing100871China

In this manuscript,we propose an analytical equivalent linear viscoelastic constitutive model for fiber-reinforced composites,bypassing general computational *** method is based on the reduced-order homogenization(ROH)*** ROH method typically involves solving multiple finite element problems under periodic conditions to evaluate elastic strain and eigenstrain influence functions in an‘off-line’stage,which offers substantial cost savings compared to direct computational homogenization *** to the unique structure of the fibrous unit cell,“off-line”stage calculation can be eliminated by influence functions obtained *** the standard solid model to the ROH method enables the creation of a comprehensive analytical homogeneous viscoelastic constitutive *** method treats fibrous composite materials as homogeneous,anisotropic viscoelastic materials,significantly reducing computational time due to its analytical *** approach also enables precise determination of a homogenized anisotropic relaxation modulus and accurate capture of various viscoelastic responses under different loading *** sets of numerical examples,including unit cell tests,three-point beam bending tests,and torsion tests,are given to demonstrate the predictive performance of the homogenized viscoelastic ***,the model is validated against experimental measurements,confirming its accuracy and reliability.

关键词： Homogenized relaxation modulus viscoelastic standard solid model reduced order homogenization fibrous composite material

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人脐静脉内皮细胞分离及冷冻保存的技术

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复旦学报（医学版） 2004年第5期31卷 509-511,514,F004页

作者：蒋红军贾兵陈张根王克强复旦大学附属儿科医院心血管中心外科复旦大学附属中山医院-上海市心血管病研究所上海200023

目的　研究分离、培养、扩增人脐静脉内皮细胞及进行冷冻保存技术 ,探讨建立脐带血管内皮细胞库的可能性。方法　新鲜脐带 4 2条 ,脐静脉内灌注消化酶消化 ,获得内皮细胞 ;以含 2 0 %的胎牛血清M 199液进行培养。采用Ⅷ因子免疫荧光染... 详细信息

目的　研究分离、培养、扩增人脐静脉内皮细胞及进行冷冻保存技术 ,探讨建立脐带血管内皮细胞库的可能性。方法　新鲜脐带 4 2条 ,脐静脉内灌注消化酶消化 ,获得内皮细胞 ;以含 2 0 %的胎牛血清M 199液进行培养。采用Ⅷ因子免疫荧光染色及扫描电镜检查 ,鉴定所获内皮细胞及其纯度 ;内皮细胞加入含 10 %DMSO冷冻保存液 ,置于液氮进行保存 ,复苏后进行锥虫蓝试验 ,四氮唑盐 (MTT)试验以及细胞凋亡的流式细胞仪检测。结果　血管内灌注消化液法可获取高纯度的内皮细胞 ,与未冻存细胞相比 ,复苏的细胞活力保持在 95 %以上。复苏后的内皮细胞的生长曲线与未冻存细胞的生长曲线无差异。冻存内皮细胞的凋亡率较未冻存细胞高 ,但无统计学意义。结论　脐静脉灌注酶消化法可获取足够数量及纯度的内皮细胞。冻存的内皮细胞复苏后仍保持较高的活力及体外增殖能力。

关键词：人脐静脉内皮细胞冻存复苏后灌注冷冻保存免疫荧光染色 Ⅷ因子锥虫生长曲线消化酶

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抗牛传染性鼻气管炎病毒gD蛋白中和活性单克隆抗体的制备及鉴定

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中国兽医科学 2019年第12期49卷 1514-1519页

作者：洪家兵刘文晓王萍李永清江西农业大学动物科学技术学院江西南昌330045 北京市农林科学院畜牧兽医研究所畜禽疫病防控技术北京市重点实验室北京100097

为了制备牛传染性鼻气管炎病毒(IBRV)gD蛋白的单克隆抗体,本研究用纯化的IBRV免疫BALB/c小鼠,然后以纯化的昆虫细胞表达的gD蛋白作为ELISA包被抗原筛选杂交瘤细胞株。结果获得3株能够稳定分泌抗gD蛋白单克隆抗体的杂交瘤细胞株1B4、3E8... 详细信息

为了制备牛传染性鼻气管炎病毒(IBRV)gD蛋白的单克隆抗体,本研究用纯化的IBRV免疫BALB/c小鼠,然后以纯化的昆虫细胞表达的gD蛋白作为ELISA包被抗原筛选杂交瘤细胞株。结果获得3株能够稳定分泌抗gD蛋白单克隆抗体的杂交瘤细胞株1B4、3E8和4F12。采用夹心ELISA鉴定单克隆抗体的Ig亚类,结果显示,1B4、3E8为IgG2b,4F12为IgG1。免疫印迹试验与间接免疫荧光试验表明,3株单克隆抗体均能与IBRV发生特异性反应。微量中和试验结果显示,1B4和3E8单克隆抗体具有中和IBRV的活性,中和效价均为1∶320。本研究成功制备了具备中和作用的抗牛传染性鼻气管炎病毒gD蛋白单克隆抗体,为建立特异性的IBRV诊断方法及致病机理的研究提供了工具。

关键词：牛传染性鼻气管炎病毒 gD蛋白单克隆抗体

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顶空固相微萃取/气相色谱法快速萃取-解吸土壤中7种芳香族化合物

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分析测试学报 2025年第1期44卷 172-179页

作者：李丙阳王钊陈佳邱洪灯新疆师范大学化学化工学院新疆乌鲁木齐830000 中国科学院兰州化学物理研究所甘肃省天然药物重点实验室甘肃兰州730000

利用氧化石墨烯功能化修饰固相微萃取(GO@SPME)针,结合气相色谱(GC)-氢火焰离子化检测器(FID)建立了检测土壤中7种挥发性芳香烃及其衍生物(包括甲苯、氯苯、乙苯、对二甲苯、邻二甲苯、硝基苯和萘)的快速萃取和解吸策略。考察了萃取液... 详细信息

利用氧化石墨烯功能化修饰固相微萃取(GO@SPME)针,结合气相色谱(GC)-氢火焰离子化检测器(FID)建立了检测土壤中7种挥发性芳香烃及其衍生物(包括甲苯、氯苯、乙苯、对二甲苯、邻二甲苯、硝基苯和萘)的快速萃取和解吸策略。考察了萃取液体积、搅拌速率、萃取温度、加盐量、解吸温度、萃取时间和解吸时间对目标物萃取效果的影响,确定在土壤样品中加入内标物质(氘代甲苯),经甲醇提取,顶空固相微萃取快速富集,再结合GC-FID进行检测,基质匹配内标法定量分析。在优化的实验条件下,该方法在15 s内可实现对目标物的快速萃取与解吸(萃取时间12 s,解吸时间3 s)。土壤样品中7种挥发性芳香烃及其衍生物的线性关系良好,相关系数(r^(2))均不小于0.9989,方法检出限(LOD,S/N=3)为1.71~11.60μg/g,定量下限(LOQ,S/N=10)为5.70~38.67μg/g;在3个不同加标水平(60、120、180μg/g)下的平均回收率为87.3%~109%,相对标准偏差(RSD)不大于12%。该方法前处理简单快速,成本较低,数据稳定可靠,适用于土壤样品中芳香族类化合物的快速检测。

关键词：芳香烃及其衍生物氧化石墨烯顶空固相微萃取气相色谱法快速萃取-解吸策略土壤

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Passive activity enhances residual control ability in patients with complete spinal cord injury

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Neural Regeneration Research 2025年第8期20卷 2337-2347页

作者： Yanqing Xiao Mingming Gao Zejia He jia Zheng hongming Bai jia-Sheng Rao Guiyun Song Wei Song Xiaoguang Li Beijing Key Laboratory for Biomaterials and Neural Regeneration Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Department of Rehabilitation Evaluation China Rehabilitation Research CenterBeijingChina Cardiac Center Beijing Children’s HospitalCapital Medical UniversityBeijingChina The State Key Laboratory of Virtual Reality Technology and Systems Beihang UniversityBeijingChina Department of Rehabilitation Engineering China Rehabilitation Science InstituteBeijingChina

Patients with complete spinal cord injury retain the potential for volitional muscle activity in muscles located below the spinal injury ***,because of prolonged inactivity,initial attempts to activate these muscles may not effectively engage any of the remaining neurons in the descending pathway.A previous study unexpectedly found that a brief clinical round of passive activity significantly increased volitional muscle activation,as measured by surface *** this study,we further explored the effect of passive activity on surface electromyographic signals during volitional control tasks among individuals with complete spinal cord *** patients with chronic complete thoracic spinal cord injury were *** electromyography data from eight major leg muscles were acquired and compared before and after the passive activity *** results indicated that the passive activity led to an increased number of activated volitional muscles and an increased frequency of *** the cumulative root mean square of surface electromyography amplitude for volitional control of movement showed a slight increase after passive activity,the difference was not statistically *** findings suggest that brief passive activity may enhance the ability to initiate volitional muscle activity during surface electromyography tasks and underscore the potential of passive activity for improving residual motor control among patients with motor complete spinal cord injury.

关键词： complete spinal cord injury cycle training epidural electrical stimulation motor training passive activity physiological state spinal cord circuit surface electromyography volitional control task

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江西省猪链球菌临床分离株毒力基因检测及药物敏感性分析

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中国兽医杂志 2018年第9期54卷 27-31页

作者：洪家兵陶政黄奕雯王萍张学良江西农业大学动物科学技术学院江西南昌330045 南昌市农村综合产权交易中心江西南昌330038

为了解江西地区猪链球菌耐药性及毒力基因的情况,本试验对2014-2016年江西地区不同规模化猪场疑似猪链球菌病的病例中,分离得到的62株猪链球菌,通过PCR和K-B纸片法对其毒力基因及药物敏感性进行检测。毒力基因检测结果显示,100%的毒株... 详细信息

为了解江西地区猪链球菌耐药性及毒力基因的情况,本试验对2014-2016年江西地区不同规模化猪场疑似猪链球菌病的病例中,分离得到的62株猪链球菌,通过PCR和K-B纸片法对其毒力基因及药物敏感性进行检测。毒力基因检测结果显示,100%的毒株含谷氨酸脱氢酶(gdh)、75. 8%的毒株含溶菌酶释放蛋白(mrp)、58. 1%的毒株含纤连蛋白和纤连蛋白原结合蛋白(fbps)、54. 8%的毒株含毒力相关序列(orf2)、53. 2%的毒株含胞外蛋白因子(epf)、22. 3%的毒株含溶血素(sly)。药物敏感性试验结果显示,对四环素、红霉素、强力霉素耐药严重,分别占96. 78%、80. 64%、80. 64%;对头孢唑啉、头孢噻污、阿莫西林敏感度较高,分别占85. 49%、87. 1%、82. 26%。

关键词：猪链球菌 PCR K-B纸片法毒力基因药敏试验

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Anomalous quality factor evolution of induced transparency in a virtualized coupled-oscillator system

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 148-155页

作者： Yunhan Yang Han jia Tuo Liu Yuzhen Yang Jun Yang Key Laboratory of Noise and Vibration Research Institute of Acoustics Chinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences

The phenomenon of induced transparency, characterized by a high quality(Q) factor, holds significant importance in the fields of high-precision sensing, energy storage and wave manipulation. Conventional transparency systems are often constrained by the limited experimentally accessible parameter space and the lack of independent control over intrinsic parameters, thereby hindering advanced research on high-Q effects. Here we propose a virtualized coupled-oscillator system that enables reconfigurable induced transparency in the reflection spectrum. By virtually incorporating a pair of coupled oscillators into an individual acoustic meta-atom, we achieve the manipulation of induced transparency in a decoupled, wide-ranging, and elaborate manner. Exploiting the diverse evolutions across the intrinsic parameter space, we show experimentally a counter-intuitive increase in the Q-factor of the transparency window, which is attributed to the enhanced dissipation of the bright ***, a constant group delay at the center frequency is observed accompanying this evolution. Our work demonstrates an intriguing platform as well as a novel strategy to engineer high-Q induced transparency phenomenon, and paves the way towards applications in programmable acoustic sensors, filters and slow-wave devices.

关键词： induced transparency acoustic meta-atom high quality-factor scattering programmable intrinsic parameter

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