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Journal of Molecular Cell Biology 2021年第10期13卷 763-766页

作者： Rikako Funabashi Kei Miyakawa Yutaro Yamaoka Seiko Yoshimura Satoshi Yamane Sundararaj Stanleyraj Jeremiah Kohei Shimizu hiroki ozawa Chiharu Kawakami Shuzo Usuku Nobuko Tanaka Etsuko Yamazaki Hirokazu Kimura Hideki Hasegawa Akihide Ryo Department of Microbiology Yokohama City University Graduate School of MedicineKanagawajapan Life Science Laboratory Technology and Development DivisionKanto Chemical Co.Inc.KanagawaJapan Primary Care Testing Solution Development Department Canon Medical Systems CorporationTochigiJapan Yokohama City Institute of Public Health KanagawaJapan Clinical Laboratory Department Yokohama City University HospitalKanagawaJapan Department of Health Science Gunma Paz University Graduate SchoolGunmajapan Center for Influenza and Respiratory Virus Research National Institute of Infectious DiseasesMusashimurayamaTokyoJapan

Ever since its outbreak,the COVID-19 pandemic caused by SARS-CoV-2 has been spreading rapidly causing a global health *** diagnosis and instituting appropriate intervention to relevant individuals are essential not only to slow down the spread of this pandemic but also to manage our resources *** date,testing for SARS-CoV-2 infection mostly relies on reverse transcription-quantitative polymerase chain reaction(RT-qPCR)on a nasopharyngeal or saliva specimen and remains the gold standard for diagnosis as it exhibits high sensitivity and specificity.

关键词： diagnosis specificity nasopharyngeal

A new sodium iron phosphate as a stable high-rate cathode material for sodium ion batteries

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Nano Research 2018年第12期11卷 6197-6205页

作者： Xiaobo Zhu Takashi Mochiku hiroki Fujii Kaibin Tang Yuxiang Hu Zhenguo Huang Bin Luo Kiyoshi ozawa Lianzhou Wang Nanomatefials Centre School of Chemical Engineering and Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandQLD 4072Australia National lnstitute for Matefials Science 1-2-1SengenTsukuba-cityIbaraki 305-0047Japan Hefei National Laboratory for Physical Science at Microscale and Department of Chemistry University of Science and Technology of ChinaHefei 230026China School of Civil and Environmental Engineering University of Technology SydneyNSW 2007Australia

Low-cost room-temperature sodium-ion batteries (SIBs) are expected to promote the development of stationary energy storage applications. However, due to the large size of Na+, most Na+ host structures resembling their Li+ counterparts show sluggish ion mobility and destructive volume changes during Na ion (de)intercalation, resulting in unsatisfactory rate and cycling performances. Herein, we report a new type of sodium iron phosphate (Na0.71Fe1.07PO4), which exhibits an extremely small volume change (~ 1%) during desodiation. When applied as a cathode material for SIBs, this new phosphate delivers a capacity of 78 mA·h·g−1 even at a high rate of 50 C and maintains its capacity over 5,000 cycles at 20 C. In situ synchrotron characterization disclosed a highly reversible solid-solution mechanism during charging/discharging. The findings are believed to contribute to the development of high-performance batteries based on Earth-abundant elements.

关键词： sodium iron phosphate low volume change cathode material long cycle rate performance sodium ion batteries

Numerical analysis on element creation by nuclear transmutation of fission products

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Nuclear Science and Techniques 2015年第1期26卷 113-120页

作者： Atsunori Terashima Masaki ozawa Department of Nuclear Engineering Graduate School of Science and EngineeringTokyo Institute of Technology Research Laboratory for Nuclear Reactors Tokyo Institute of Technology

A burnup calculation was performed to analyze the Apr`es ORIENT process, which aims to create highlyvaluable elements from fission products separated from spent nuclear fuels. The basic idea is to use nuclear transmutation induced by a neutron capture reaction followed by a β-decay, thus changing the atomic number Z of a target element in fission products by 1 unit. LWR(PWR) and FBR(MONJU) were considered as the transmutation devices. High rates of creation were obtained in some cases of platinum group metals(44Ru by FBR,46 Pd by LWR) and rare earth(64Gd by LWR,66 Dy by FBR). Therefore, systems based on LWR and FBR have their own advantages depending on target elements. Furthermore, it was found that creation rates of even Z(= Z + 1) elements from odd Z ones were higher than the opposite cases. This creation rate of an element was interpreted in terms of "average 1-group neutron capture cross section of the corresponding target element σc Z defined in this work. General trends of the creation rate of an even(odd) Z element from the corresponding odd(even) Z one were found to be proportional to the 0.78th(0.63th) power of σc Z, however with noticeable dispersion. The difference in the powers in the above analysis was explained by the difference in the number of stable isotopes caused by the even-odd effect of Z.

关键词：裂变产物元素数值分析嬗变快中子增殖反应堆轻水反应堆稳定同位素 LWR

Resourceability on nuclear fuel cycle by transmutation approach

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Science China Chemistry 2012年第9期55卷 1746-1751页

作者： ozawa Masaki SAITO Masaki Research Laboratory for Nuclear Reactors Tokyo Institute of Technology2-12-1-N1-1 OokayamaMeguro-kuTokyo 152-8550Japan

A resourceability on nuclear fuel cycle by transmutation of fission products in the spent fuel of nuclear reactors is discussed in this paper to investigate the feasibility of ＂creation and utilization＂ of Apr6s ORIENT from Adv.-ORIENT cycle, in which chemical ＂separation and utilization＂ of nuclear rare metals （platinum group metals, Mo, Tc, rare earth, etc.） has been proposed since FY2006. Apr6s ORIENT research program was newly initiated in FY2011 for nuclear transmutation of fission products into stable or short-lived highly-valuable elements. In the resourceability of rare earth metals from fission products, non-radioactive Nd and Dy can be created from Pr and Tb, respectively, by transmutation. Especially, the Dy creation has relatively high feasibility of about 10-20 %/y in creation rate. A proper moderation of neutrons in blanket of fast reactors may be required to provide a high creation rate of La from Ba. In light platinum group metals, non-radioactive Ru can be created from Tc by transmutation, of which creation rate is about 4-5 %/y in blanket of fast reactors. Pd created from Rh is almost non-radioactively depending on the isotope fraction of 107pd. Rh creation from Ru is not feasible under the neutron irradiation of typical nuclear reactors.

关键词： Apres ORIENT Advanced ORIENT cycle nuclear rare metal transmutation nuclear fuel cycle

Deobfuscation,unpacking,and decoding of obfuscated malicious JavaScript for machine learning models detection performance improvement

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CAAI Transactions on Intelligence Technology 2020年第3期5卷 184-192页

作者： Samuel Ndichu Sangwook Kim Seiichi ozawa Graduate School of Engineering Kobe UniversityKobe CityJapan Center for Mathematical and Data Sciences Kobe UniversityKobe CityJapan

Obfuscation is rampant in both benign and malicious JavaScript(JS)*** generates an obscure and undetectable code that hinders comprehension and ***,accurate detection of JS codes that masquerade as innocuous scripts is *** existing deobfuscation methods assume that a specific tool can recover an original JS code *** a multi-layer obfuscation,general tools realize a formatted JS code,but some sections remain *** the detection of such codes,this study performs Deobfuscation,Unpacking,and Decoding(DUD-preprocessing)by function redefinition using a Virtual Machine(VM),a JS code editor,and a python int_to_str()function to facilitate feature learning by the FastText *** learned feature vectors are passed to a classifier model that judges the maliciousness of a JS *** performance evaluation,the authors use the Hynek Petrak’s dataset for obfuscated malicious JS codes and the SRILAB dataset and the Majestic Million service top 10,000 websites for obfuscated benign JS *** then compare the performance to other models on the detection of DUD-preprocessed obfuscated malicious JS *** experimental results show that the proposed approach enhances feature learning and provides improved accuracy in the detection of obfuscated malicious JS codes.

关键词： JavaScript editor python

Optogenetic Control of Phosphatidylinositol (3,4,5)-Triphosphate Production by Light-Sensitive Cryptochrome Proteins on the Plasma Membrane

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Chinese Journal of Chemistry 2021年第5期39卷 1240-1246页

作者： Lingzhi Yang Takeaki ozawa Haifeng Dong Xueji Zhang School of Pharmaceutical Sciences Health Science CenterShenzhen UniversityShenzhenGuangdong 518060China Deportment of Chemistry School of ScienceThe University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan School of Biomedical Engineering Health Science CenterShenzhen UniversityShenzhenGuangdong 518060China

Phosphatidylinositol(3,4,5)-triphosphate(PIP3),acting as a fundamental second messenger,is emerging as a promising biomarker for disease diagnosis and ***,the real time analysis of phosphoinositide in living cells remains a key challenge owing to the low basal abundance and its fast metabolic ***,we design an optogenetic system that uses light sensitive protein-protein interaction between Arabidopsis cryptochrome 2(CRY2)and CIB1 to spatiotemporally visualize the PIP3 production with sub-second *** this system,a CIBN is anchored on the plasma membrane,whereas a CRY2 fused with a constitutively active PI3-kinase(acPI3K)would be driven from the cytosol to the membrane by the blue-light-activated CRY2-CIB1 interaction upon light *** PIP3 production is visualized via a fused fluorescent protein by the translocation of a Pleckstrin Homology(PH)domain(GRP1)from the cytosol to the plasma membrane with high *** demonstrated the fast dynamics and reversibility of the optogenetic system initiated PIP3 synthesis on the plasma ***,the real-time cell movements were also observed upon localized light *** established optogenetic method provides a novel spatiotemporal strategy for specific PIP3 visualization,which is beneficial to improve the understanding of PIP3 functions.

关键词： Protein-protein interactions Protein engineering Fluorescent probes Optogenetic control Phosphatidylinosito (3,4,5)-triphosphate

Additive Manufacturing of Metal Micro-ring and Tube by Laser-Assisted Electrophoretic Deposition with Laguerre-Gaussian Beam

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Nanomanufacturing and Metrology 2021年第4期4卷 271-277页

作者： Kenta Nakazawa Syunya ozawa Futoshi Iwata Graduate School of Integrated Science and Technology Shizuoka University3-5-1 Johoku.Naka-kuHamamatsu 432-8561.Japan Graduate School of Medical Photonics Shizuoka University3-5-1 JohokuNaka-kuHamamatsu 432-8011Japan Research Institute of Electronics Shizuoka University3-5-1 JohokuNaka-kuHamamatsu 432-8011Japan

We report a method for laser-assisted electrophoretic deposition using a Laguerre-Gaussian beam for maskless patterning of metal rings and *** are structures of utmost importance,particularly in photonic *** nanoparticles,which are gathered in a colloidal solution by a laser trapping technique,are deposited on a substrate via *** deposition pattern is dependent on the focused spot shape during laser *** intensity distribution of the Laguerre-Gaussian beam is *** with different inner diameters can be fabricated by varying the topological charge of the Laguerre-Gaussian *** equivalent inner diameters of the deposited rings with topological charges of 1,3,and 5 were 0.30,0.78,and 1.45μm,*** inner diameter of the deposited ring with a topological charge of 1 was smaller than the wavelength of the laser beam(532 nm).A tube was also fabricated with a topological charge of 3 by vertical displacement of the deposition *** deposition technology developed here using Laguerre-Gaussian beam will contribute to advancements in the fabrication of photonic and nano-fluidic devices.

关键词： Additive manufacturing Laguerre-Gaussian beam Laser trapping Maskless fabrication Electrophoretic deposition

Van Vleck paramagnetism of europium oxyhydroxide

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Journal of Rare Earths 2015年第2期33卷 177-181页

作者： Hiroaki Samata Naoki Wada Tadashi C.ozawa Graduate School of Maritime Sciences Kobe University FukaeminamiHigashinada Kobe Hyogo 658-0022 Japan International Center for Materials Nanoarchitectonics National Institute for Materials ScienceNamiki Tsukuba Ibaraki 305-0044 Japan

Europium oxyhydroxide crystals were synthesized by the flux method. The as-grown crystals were transparent and had a plate-like shape with natural flat surfaces. The powder XRD data were refined by assuming a monoclinic structure of the space group P21/m and lattice parameters of a=0.4346 nm, b=0.3744 nm, c=0.6107 nm, and β=108.62°. The magnetic susceptibility of the EuOOH crystals exhibited typical Van Vleck temperature-independent paramagnetism below 120 K. The calculated susceptibility, based on Van Vleck＇s theory, agreed with the experimental data to some extent, with the coupling constant λ=458±10 K. The experimental results were in close agreement with the results calculated using a modified formula with λ=505±2 K and a constant term C=4.6×10^-4 emu/（mol·Oe）.

关键词： europium oxyhydroxide magnetic measurement Van Vleck paramagnetism rare earths

Efficient high harmonics generation by enhancement cavity driven with a post-compressed FCPA laser at 10 MHz

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High Power Laser Science and Engineering 2018年第2期6卷 45-50页

作者： Zhigang Zhao Akira ozawa Makoto Kuwata-Gonokami Yohei Kobayashi The Institute for Solid State Physics The University of Tokyo Department of Physics Graduate School of Science The University of Tokyo

Efficient high harmonics generation(HHG) was demonstrated at 10 MHz repetition rate with an external femtosecond enhancement cavity, seeded by a ～70 fs post-compressed 10 MHz fiber chirped pulse amplifier(FCPA) laser. Operation lasting over 30 min with 0.1 m W outcoupled power at 149 nm was demonstrated. It was found that shorter pulse was beneficial for alleviating the nonlinear plasma effect and improving the efficiency of HHG. Low finesse cavity can relax the plasma nonlinearity clamped intra-cavity power and improve the cavity-locking stability. The pulse duration is expected to be below 100 fs for both 1040 nm and 149 nm outputs, making it ideal for applications such as time-resolved photoemission spectroscopy.

关键词： enhancement cavity high harmonics generation ultrafast fiber lasers

Systemic RNAi of V-ATPase subunit B causes molting defect and developmental abnormalities in Periplaneta fuliginosa

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Insect Science 2019年第4期26卷 721-731页

作者： Kazuki Sato Keita Miyata Sota ozawa Koichi Hasegawa Laboratory of Nematology Department of Applied Biological Sciences Saga University Honjo 1 Saga Japan The United Graduate School of Agricultural Sciences Kagoshima University Korimoto 1-21-24 Kagoshima Japan Department of Biomedical Sciences College of Life and Health Sciences Chubu University Kasugai Aichi Japan Department of Environmental Biology College of Bioscience & Biotechnology Chubu University Kasugai Aichi Japan

The vacuolar (H+)-ATPases (V-ATPases) are ATP-driven proton pumps with multiple functions in many organisms. In this study, we performed structural and functional analysis of vha55 gene that encodes V-ATPase subunit B in the smokybrown cockroach Periplaneta fuliginosa (Blattodea). We observed a high homology score of the deduced amino acid sequences between 10 species in seven orders. RNAi of the vha55 gene in R fuliginosa caused nymphal/nymphal molting defects with incomplete shedding of old cuticles, growth inhibition, as well as bent and wrinkled cuticles of thoraxes and abdominal segments. Since growth inhibition caused by vha55 RNAi did not interfere in the commencement of cockroach molting, molting timing and body growth might be controlled by independent mechanism. Our study suggested V-ATPases might be a good candidate molecule for evolutionary and developmental studies of insect molting.

关键词： Blattodea cockroach molting Periplaneta fuliginosa RNA interference vacuolar-type H+-ATPase