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Journal of Materials Science and Chemical Engineering 2017年第1期5卷 42-47页

作者： hiroki nakaie Tetsuji Arai Chiaya Yamamoto Keisuke Arimoto Junji Yamanaka Kiyokazu Nakagawa Toshiyuki Takamatsu Interdisciplinary Graduate School of Medicine and Engineering University of Yamanashi Kofu Japan SST Inc. Yachiyo Japan

We have developed a microwave plasma heating technique to rapidly heat the transition metal. W/SiO2 layers were deposited on Ge/Si heterostructures. By heating the W, dislocations in Ge layers originated from lattice mismatch between Ge and Si crystals were reduced drastically. We have fabricated p- MOSFETs on Ge/Si substrates and realized higher mobility of about 380 cm2/ Vs than that of Si p-MOSFET.

关键词： Microwave Plasma Heating High Hole Mobility Ge on Si

Formation of Poly-Si Films on Glass Substrates by Using Microwave Plasma Heating and Fabrication of TFT’s on the Films

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Journal of Materials Science and Chemical Engineering 2018年第1期6卷 19-24页

作者： hiroki nakaie Tetsuji Arai Keisuke Arimoto Junji Yamanaka Kiyokazu Nakagawa Kazuki Kamimura Toshiyuki Takamatsu Interdisciplinary Graduate School of Medicine and Engineering University of Yamanashi Kofu Japan SST Inc. Yachiyo Japan

We have developed an apparatus for producing high-density hydrogen plasma. The atomic hydrogen density was 3.0 × 1021 m?3 at a pressure of 30 Pa, a microwave power of 1000 W, and a hydrogen gas flow rate of 5 sccm. We confirmed that the temperatures of tungsten films increased to above 1000?C within 5 s when they were exposed to hydrogen plasma formed using the apparatus. We applied this phenomenon to the selective heat treatment of tungsten films deposited on amorphous silicon films on glass substrates and formed polycrystalline silicon films. To utilize this method, we can perform the crystalline process only on device regions. TFTs were fabricated on the polycrystalline silicon films and the electron mobilities of 60 cm2/Vs were obtained.

关键词： Microwave Plasma Heating Poly-Si Thin Film Transistor

STEM MoiréObservation of Lattice-Relaxed Germanium Grown on Silicon

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Journal of Materials Science and Chemical Engineering 2017年第1期5卷 102-108页

作者： Junji Yamanaka Chiaya Yamamoto hiroki nakaie Tetsuji Arai Keisuke Arimoto Kosuke O. Hara Kiyokazu Nakagawa Center for Crystal Science and Technology University of Yamanashi Kofu Japan Center for Creative Technology University of Yamanashi Kofu Japan Center for Instrumental Analysis University of Yamanashi Kofu Japan

We deposited Ge films on Si substrates by molecular beam epitaxy (MBE) method. The specimens were annealed at around 750 C using microwave- plasma heating technique which we had reported before. After these pro- cesses, we carried out special scanning transmission electron microscopic (STEM) observation. The moiré between the crystal lattices and the scanning lines controlled by STEM was utilized to show lattice-spacing distribution. The results exhibited that we were succeeded in forming lattice-relaxed Ge thin films. It was also recognized that this STEM moiré technique is very useful to observe lattice-spacing distribution for large area with high resolution.

关键词： STEM Moiré Lattice Strain Ge on Si Plasma Heating

Selective Heating of Transition Metal Usings Hydrogen Plasma and Its Application to Formation of Nickel Silicide Electrodes for Silicon Ultralarge-Scale Integration Devices

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Journal of Materials Science and Chemical Engineering 2016年第1期4卷 29-33页

作者： Tetsuji Arai hiroki nakaie Kazuki Kamimura Hiroyuki Nakamura Satoshi Ariizumi Satoki Ashizawa Keisuke Arimoto Junji Yamanaka Tetsuya Sato Kiyokazu Nakagawa Toshiyuki Takamatsu Interdisciplinary Graduate School of Medicine and Engineering University of Yamanashi Kofu Japan SST Inc. Yachiyo Japan

We developed an apparatus for producing high-density hydrogen plasma. The atomic hydrogen density was 3.1 × 1021 m?3 at a pressure of 30 Pa, a microwave power of 1000 W, and a hydrogen gas flow rate of 10 sccm. We confirmed that the temperatures of transition-metal films increased to above 800。C within 5 s when they were exposed to hydrogen plasma formed using the apparatus. We applied this phenomenon to the selective heat treatment of nickel films deposited on silicon wafers and formed nickel silicide electrodes. We found that this heat phenomenon automatically stopped after the nickel slicidation reaction finished. To utilize this method, we can perform the nickel silicidation process without heating the other areas such as channel regions and improve the reliability of silicon ultralarge-scale integration devices.

关键词： Selective Heating Nickel Silicide Electrode Hydrogen Plasma Microwave Plasma

Angiotensin-(1-7)Changes Apoptosis-Related Genes Expression in Human Breast Cancer Cell Line T47D

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Journal of Cancer Therapy 2014年第14期5卷 1412-1422页

作者： Cheryl Alecrim Santos Gabriela Soares da Silva Brito Silvana Aparecida Alves Correa de Noronha Samuel Marcos Ribeiro de Noronha Suma Imura Shimuta Clovis Ryiuchi nakaie Ismael Dale Cotrim Guerreiro da Silva Department of Gynecology Federal University of Sao PauloSao PauloBrazil Department of Surgery Federal University of Sao PauloSao PauloBrazil Department of Biophysics Federal University of Sao PauloSao PauloBrazil

Angiotensin-(1-7) [Ang-(1-7)] is a heptapeptide of the renin-angiotensin system with vasodilator and anti-proliferative properties. In the present study, we aim to investigate whether Ang-(1-7) induces apoptosis in breast cancer cells and whether the altered expression of apoptosis-related genes is involved in this process. Human breast cell line T47D was treated with angiotensin-(1-7) and angiotensin II (Ang II). Cell proliferation and apoptosis were quantified using hemocytometer and flow cytometry, respectively. The expression of 84 apoptosis-related genes was evaluated through qPCR array. Ang-(1-7), as opposed to Ang II, decreased proliferation and increased apoptosis in T47D cells. Moreover, many pro-apoptotic genes were up-regulated, such as BAK1, BAX, BCL2L1, BID and BIK. In addition, some anti-apoptotic genes as AKT1 and XIAP were down-regulated by heptapeptide. Although a deeper study should be performed, our results support the hypothesis that Ang-(1-7) could change the expression of several genes related to apoptosis, interfering directly in the molecular pathways associated with the survival of breast cancer cells.

关键词： Angiotensin-(1-7) Breast Cancer Cells Apoptosis qPCR Array

Effects on Cell Viability and on Apoptosis in Tumoral(MCF-7)and in Normal(MCF10A)Epithelial Breast Cells after Human Chorionic Gonadotropin and Derivated-Angiotensin Peptides Treatments

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Journal of Cancer Therapy 2013年第7期4卷 65-69页

作者： Silvana Aparecida Alves Correa de Noronha Werica Bernardo Alexandre Jesus Barros Clovis Ryuichi nakaie Suma Imura Shimuta Ismael Dale Cotrim Guerreiro da Silva Samuel Marcos Ribeiro de Noronha Laboratorio de Cirurgia Translacional Departamento de CirurgiaUniversidade Federal De Sao Paulo/Escola Paulista De MedicinaSao PauloBrazil Laboratorio de Ginecologia Molecular Universidade Federal de Sao PauloSao PauloBrazil Departamento de Biofísica Universidade Federal de Sao PauloSao PauloBrazil

Angiotensin-(1 - 7) [Ang-(1 - 7)] is an endogenous heptapeptide hormone of the renin-angiotensin system that has antiproliferative properties. The aim of this work was to evaluate the anti-proliferative and pro-apoptotic properties of Ang-(1 - 7) and of Ang-(1 - 7)-substituents 9-fluorenylmethyloxycarbonyl (Fmoc) e Ang II-derivatives containing the TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid) in normal (MCF10A) and in tumoral (MCF7) epithelial mammary cell lines. Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combination or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. We also observed cell viability decrease in MCF10A after Ang-(1 - 7), Ang-(1 - 7) Fmoc and hCG + AngII Toac treatments. These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. Regarding the cell cycle analysis, we did not observed any changes in cell cycle phases. In summary, cell viability was decreased and apoptosis (initial, mid and late) was increased after hCG and/or Ang-(1 - 7) peptides treatments. These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. Our results support the idea of investigating more closely the putative use of these compounds as novel therapeutic agents for breast cancer.

关键词： Angiotensin II Angiotensin 1-7 Angiotensin II Type 1 Receptor(AT1R) Breast Cancer Apoptosis Human Chorionic Gonadotropin

Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and <i>AGTR</i>2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression

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Journal of Cancer Therapy 2013年第9期4卷 1403-1410页

作者： Maria Del Carmen Garcia Molina Wolgien Ismael Dale Cotrim Guerreiro da Silva Afonso Celso Pinto Nazário Clovis Riyuchi nakaie Silvana Aparecida Alves Corrêa de Noronha Samuel Marcos Ribeiro de Noronha Gil Facina Biophisics Department Escola Paulista de Medicina da Universidade Federal de Sao Paulo (EPM-UNIFESP) Sao Paulo Brazil Gynecology Department Escola Paulista de Medicina/Universidade Federal de Sao Paulo (EPM-UNIFESP) Sao Paulo Brazil Surgery Department Escola Paulista de Medicina/Universidade Federal de Sao Paulo (EPM-UNIFESP) Sao Paulo Brazil

Background: Breast cancer is the most common type of cancer among women. Diagnosed and treated timely, patients may have good prognostics. In Brazil, in 2012, the estimate of new cases was 52,680 and the number of registered deaths in 2012 was 12,852. The Renin-Angiotensin System (RAS) is known for its role in arterial hypertension and in other cardiovascular diseases. Angiotensin-Converting Enzyme 2 (ACE2) is the key to Ang-(1-7) formation, and counterbalances the ACE1/AngII/AGTR1 axis actions. RAS components have complex interactions with different tissues and their actions are not restricted to the cardiovascular system. Recently, the RAS has been associated with different types of cancers and in particular with gynecological cancers. Objectives: Our aim is to investigate possible associations between allelic distribution of two genetic polymorphisms in the AGTR2 receptor with ACEs 1 and 2 plasma levels among women with breast cancer. Patients and Methods: Patients with breast cancer were genotyped for two polymorphisms of the AGTR2 (T1247G and A5235G). Genotyping assays (TaqMan) were performed with genomic DNA extracted from blood cells. ACEs plasma level measurements were conducted in women from the breast-cancer group (N = 53). ACEs were measured in the plasma of these patients using ELISA kits. Results: SNPs genotype distribution is correlated with ACEs plasma levels. ACEs plasma levels are also correlated with clinical variables and ACE2 high levels are associated with better prognostics. Conclusions: Changes in circulating levels of ECA1/AngII ECA2/ Ang-(1-7) determine the magnitude of the inflammatory response that an individual can trigger and the variation in ACE 1 and 2 plasma level measurements in the blood of breast cancer patients suggests an association with the process of mammary carcinogenesis. Thus, the RAS may be associated with the process of mammary carcinogenesis by both genotypic variations of RAS components and by circulating levels of ACEs

关键词： Angiotensin-Converting Enzyme 1 Angiotensin-Converting Enzyme 2 Angiotensin II Type 2 Receptor Breast Neoplasm ACEs Plasma Level Genetic Polymorphisms

Angiotensin-(1-7)and Human Chorionic Gonadotropin(hCG)Modulate the Nuclear Transcription Factors or Nuclear Receptors Genes in the Tumorigenic Undifferentiated Breast Cancer Cell Line SKBR3

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Journal of Cancer Therapy 2013年第7期4卷 70-74页

作者： Isidoro Binda Neto Samuel Marcos Ribeiro de Noronha Silvana Aparecida Alves Correa de Noronha Maria Del Carmen Garcia Molina Wolgien Alexandre Jesus Barros Clovis Ryiuchi nakaie Suma Imura Shimuta Gil Facina Ismael Dale Cotrim Guerreiro da Silva Laboratorio de Ginecologia Molecular Universidade Federal de Sao PauloSao PauloBrazil Universidade Federal do Estado do Rio de Janeiro Rio de JaneiroBrazil Laboratorio de Cirurgia Translacional Departamento de CirurgiaUNIFESP/EPMSao PauloBrazil Departamento de Biofísica Universidade Federal de Sao PauloSao PauloBrazil

Breast cancer is the most common cancer among women. Angiotensin-(1 - 7) [Ang-(1 - 7)] has been correlated with cancer antiproliferative and apoptotic effects, similar properties of the human Chorionic Gonadotrofin (hCG). The aims of this work are to evaluate the role of Ang-(1 - 7) and of hCG in modulating the expression of Nuclear Receptors and Coregulators related genes in the tumorigenic breast cell line SK-BR3. Three experimental groups were created: control, hCG and hCG + Ang-(1 - 7). Cells were treated for 11 days and then had their RNA extracted. Samples were loaded into PCR Array plates containing 84 genes relate to Nuclear Receptors and Coregulators pathways. Gene expression data were used to construct canonical pathways (MetacoreTM). hCG and hCG + Ang-(1 - 7) treatments markedly modulate the expression of Nuclear Receptors and Coregulators related genes. hCG differentially expressed 17% of the genes, being 29% upregulated and 71% downregulated. Meanwhile, hCG + Ang-(1 - 7) changed the expression of 30% of the genes on the plate, among these genes 56% were upregulated and 44% downregulated. Among these differentially expressed genes, we highlight Esr1, Nr2f2, and Nr2f1, Esr1, Hdac5, and Nr4A1 (>4 fold). Finally MetaCore analysis based on Gene Ontology (GO) generated six networks for hCG and ten networks for the combined treatment. All generated networks are related to regulation of apoptosis or to Programmed Cell Death processes. In summary, our results herein demonstrate that the modulation of sexual hormones and of other nuclear factor genes expression might underlie the tumorigenic protection effect and the induction of cell differentiation caused by the hormones hCG and Ang-(1 - 7), especially in Cancer Stem Cells.

关键词： Breast Stem Cancer Cells SK-BR3 hCG Angiotensin-(1-7)