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Organophosphate pesticides an emerging environmental contaminant: Pollution, toxicity, bioremediation progress, and remaining challenges

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Journal of Environmental Sciences 2023年第5期127卷 234-250页

作者： himanshu mali Chandni Shah B.H.Raghunandan Anil S.Prajapati Darshan H.Patel Ujjval Trivedi R.B.Subramanian P.G.Department of Biosciences UGC-Centre of Advanced StudiesSatellite CampusSardar Patel UniversitySardar Patel MaidanBakrol-Vadtal RoadBakrol 388315GujaratIndia Charotar Institute of Paramedical Sciences Charotar University of Science and Technology(CHARUSAT)Changa 388421GujaratIndia

Organophosphates(OPs)are an integral part of modern agriculture;however,due to overexploitation,OPs pesticides residues are leaching and accumulating in the soil,and groundwater contaminated terrestrial and aquatic food *** exposure to OPs could produce toxicity in insects,plants,animals,and *** are known for covalent inhibition of acetylcholinesterase enzyme in pests and terrestrial/aquatic organisms,leading to nervous,respiratory,reproductive,and hepatic *** pesticides also disrupt the growth-promoting machinery in plants by inhibiting key enzymes,permeability,and trans-cuticular diffusion,which is crucial for plant *** use of OPs,directly/indirectly affecting human/environmental health,raise a thoughtful global *** a safe,reliable,economical,and eco-friendly methods for removing OPs pesticides from the environment is thus *** techniques coupled with microbes or microbial-biocatalysts are emerging as promising antidotes for OPs ***,we comprehensively review the current scenario of OPs pollution,their toxicity(at a molecular level),and the recent advancements in biotechnology(modified biocatalytic systems)for detection,decontamination,and bioremediation of OP-pesticides in polluted ***,the review focuses on onsite applications of OPs degrading enzymes(immobilizations/biosensors/others),and it also highlights remaining challenges with future approaches.

关键词： Organophosphorus compounds Toxicity Bioremediation Microorganisms Enzymes Biosensors

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EXISTENCE,MULTIPLICITY AND STABILITY RESULTS FOR POSITIVE SOLUTIONS OF NONLINEAR p-LAPLACIAN EQUATIONS

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Chinese Annals of Mathematics,Series B 2004年第2期25卷 275-286页

作者： mali SUNING DepartmentofMathematicalSciences TsinghuaUniversityBeijing100084China.

This paper studies the existence of positive solutions of the Dirichlet problem for the nonlinear equation involving p-Laplacian operator: -△pu = λf(u) on a bounded smooth domain Ω in Rn. The authors extend part of the Crandall-Rabinowitz bifurcation theory to this problem. Typical examples are checked in detail and multiplicity of the solutions are illustrated. Then the stability for the associated parabolic equation is considered and a Fujita-type result is presented.

关键词： p-Laplacian operator Bifurcation Multiplicity Positive solutions

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Thermal Stability of Supercapacitor for Hybrid Energy Storage System in Lightweight Electric Vehicles:Simulation and Experiments

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Journal of Modern Power Systems and Clean Energy 2022年第1期10卷 170-178页

作者： Vima mali Brijesh Tripathi Department of Electrical Engineering/Solar Energy Pandit Deendayal Petroleum UniversityGandhinagarIndia Department of Science Pandit Deendayal Petroleum UniversityGandhinagarIndia

Recent research findings indicate that the non-monotonic consumption of energy from lithium-ion(Li-ion)batteries results in a higher heat generation in electrical energy storage *** peak demands,a higher heat generation due to high discharging current increases the temperature from 80℃ to 120℃,thereby resulting in thermal *** address peak demands,an additional electrical energy storage component,namely supercapacitor(SC),is being investigated by various research *** paper provides insights into the capability of SCs in lightweight electric vehicles(EVs)to address peak demands using the worldwide harmonized light-duty driving test cycle(WLTC)driving profile in MATLAB/Simulink at different ambient *** results indicate that temperature imposes a more prominent effect on Li-ion batteries compared with SCs under peak demand *** effect of the discharging rate limit on the Li-ion battery current is *** result shows that SCs can accommodate the peak demands for a low discharging current limit on the battery,thereby reducing heat *** impedance spectroscopy and cyclic voltammetry are performed on SCs to analyze their thermal performance at different temperatures ranging from 0℃ to 75℃ under different bias values of-0.6,0,0.6,and 1 V,*** results indicate a higher specific capacitance of the SC at an optimum operation temperature of 25℃ for the studied *** study shows that the hybrid combination of the Li-ion battery and SC for a lightweight EV can address peak demands by reducing thermal stress on the Li-ion battery and increasing the driving range.

关键词： Electric vehicle lithium-ion battery supercapacitor thermal stability

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Event-Triggered Sampled-Data Consensus of Nonlinear Multi-Agent Systems with Control Input Losses

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Journal of Systems Science & Complexity 2018年第6期31卷 1469-1497页

作者： XING mali DENG Feiqi Department of Automation Science and Engineering South China University of Technology

This paper investigates the leader-following consensus problem for multi-agent systems with event-triggered mechanism and control packet *** on the local synchronization error, event-triggered mechanisms are proposed in order to reduce the number of controller *** control packet may lose due to unreliability of communication *** the assumption that once the packet loss happens the controller will be set to zero,sufficient consensus criteria for multi-agent system with event-triggered mechanism and control packet losses is *** is also shown that the interplay among the allowable packet loss rate,event-triggered mechanism and sampling *** illustrative example is given to demonstrate the effectiveness of the theoretical results.

关键词： Consensus control packet losses event-triggered mechanism multi-agent systems

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Pancreatitis in pregnancy: etiology, diagnosis, treatment, and outcomes

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Hepatobiliary & Pancreatic Diseases International 2016年第4期15卷 434-438页

作者： Padmavathi mali Department of Internal Medicine Marshfield Clinic

BACKGROUND：Acute pancreatitis in pregnancy is a rare and dangerous *** study aimed to examine the etiology,treatment,and outcomes of pancreatitis in ***：A total of 25 pregnant patients diagnosed with pancreatitis during the period of 1994 and 2014 was analyzed ***：The pregnant patients were diagnosed with pancreatitis during a period of 21 ***（60%）of the patients were diagnosed with pancreatitis in the third *** mean age of the patients at presentation was 25.7 years,with a mean gestational age of 24.4 *** pain occurred in most patients and vomiting in one patient was associated hyperemesis *** common cause of the disease was gallstone-related（56%）,followed by alcohol-related（16%）,post-ERCP（4%）,hereditary（4%）and undetermined conditions（20%）.The level of triglycerides was minimally high in three *** and wire-guided sphincterotomy were performed in 6（43%）of 14 patients with gallstone-related pancreatitis and elevated liver enzymes with no ***（84%）of the patients underwent a full-term,vaginal *** was no difference in either maternal or fetal outcomes after ***：Acute pancreatitis is rare in pregnancy,occurring most commonly in the third trimester,and gallstones are the most common *** laparoscopic cholecystectomy is not feasible and a common bile duct stone is highly suspected on imaging,endoscopic sphincterotomy or stenting may help to prevent recurrence and postpone cholecystectomy until after delivery.

关键词： pancreatitis alcoholic pancreatitis pregnancy complications diagnostic imaging

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Photocatalytic activity of metal nanoparticle-decorated titanium dioxide for simultaneous H_(2) production and biodiesel wastewater remediation

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Chinese Journal of Chemical Engineering 2021年第8期34卷 86-100页

作者： Patsakol Prayoonpunratn Trin Jedsukontorn mali Hunsom Fuels Research Center Department of Chemical TechnologyFaculty of ScienceChulalongkorn UniversityBangkok 10330Thailand Current address:Department of Chemical Engineering Faculty of EngineeringMahidol UniversityNakorn Pathom 73170Thailand Associate Fellow of Royal Society of Thailand(AFRST) Bangkok 10300Thailand

A set of metal nanoparticle-decorated titanium dioxide(Mx/TiO_(2);where x is the percent by mass,%)photocatalysts was prepared via the sol-immobilization in order to enhance the simultaneous hydrogen(H_(2))production and pollutant reduction from real biodiesel *** of the metal nanoparticle(NP)type(M=Ni,Au,Pt or Pd)and,for Pd,the amount(1%–4%)decorated on the surface of thermal treated commercial TiO_(2)(T_(400))was *** obtained results demonstrated that both the type and amount of decorated metal NPs did not significantly affect the pollutant reduction,measured in terms of the reduction of chemical oxygen demand(COD),biological oxygen demand(BOD)and oil&grease levels,but they affected the H_(2) production rate from both deionized water and biodiesel wastewater,which can be ranked in the order of Pt_(1)/T_(400)>Pd_(1)/T_(400)>Au_(1)/T_(400)>Ni_(1)/T_(400).This was attributed to the high difference in work function between Pt and the parent ***,the difference between Pt1/T400 and Pd1/T400 was not great and so from an economic consideration,Pd/TiO_(2) was selected as appropriate for further *** the four different Pdx/TiO_(2) photocatalysts,the Pd3/TiO_(2) demonstrated the highest activity and gave a high rate of H_(2) production(up to 135 mmol·h−1)with a COD,BOD and oil&grease reduction of 30.3%,73.7%and 58.0%,respectively.

关键词： Hydrogen production Wastewater Remediation Photochemistry Metal nanoparticle-decorated titanium dioxide

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Bioconcentration of polycyclic aromatic hydrocarbons in roots of three mangrove species in Jiulong River Estuary

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Journal of Environmental Sciences 2005年第2期17卷 285-289页

作者： LUZhi-qiang ZHENGWen-jiao mali SchoolofLifeSciences ResearchCentreforWetlandandEcologicalEngineeringXiamenUniversityXiamen361005China／／EnvironmentalScienceResearchCenterXiamenUniversityXiamen361005China SchoolofLifeSciences ResearchCentreforWetlandandEcologicalEngineeringXiamenUniversityXiamen361005China EnvironmentalScienceResearchCenter XiamenUniversityXiamen361005China

The polycyclic aromatic hydrocarbons(PAHs) concentrations were determined in the root of three mangrove species(Kandelia candel, Avicennia marina and Bruguiera gymnorrhiza) and their growing environment(sediment) in mangrove wetlands of Jiulong River Estuary, Fujian, China. The total PAHs(16 parent PAHs) in mangrove sediments ranged from 193.44 to 270.53 ng/g dw, with a mean value of 231.76±31.78 ng/g dw. Compared with other mangrove and coastal marine sediments, the PAHs concentrations of all the sampling areas in this study were at relatively lower level. The total PAHs(13 parent PAHs) values varied from 30.83 to 62.73 ng/g dw in mangrove roots. Benzo[a]pyrene(five-ring), fluoranthene(four-ring) and pyrene(four-ring) dominated in mangrove sediments. Based on ratios of phenathrene/anthracene, fluoranthene/pyrene and fluoranthene/pyrene + fluoranthene, the main possible sources of surface sediment PAHs were identified as grass, wood or coal combustion for mangrove wetlands of Jiulong River Estuary. Naphthalene(two-ring) and phenathrene(three-ring) were the most abundant compounds in mangrove roots. Sediment-to-vegetation bioconcentration factors(BCF SV s) were calculated and their relationships with PAHs' physico-chemical properties were investigated. The average BCF SV s of PAHs for three mangrove species roots were almost all under the level of 1 except for naphthalene. Good linear relationship between BCF SV values for mangrove roots and PAHs water solubility, octanol-water partitioning coefficients was derived in present study. The solubility and the octanol-water partition coefficient were proved to be good predictors for the accumulation of PAHs in mangrove roots, respectively.

关键词： mangrove PAHs Jiulong River Estuary bioconcentration factors

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Expression of Human Vascular Endothelial Growth Factor (VEGF_(165)) in Pichia pastoris and Its Biological Activity

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Biomedical and Environmental Sciences 2001年第4期14卷 302-311页

作者： mali ZHANGZHI－QING 等 InstituteofMolecularImmunology theFirstMilitaryMedicalUniversityGuangzhou510515China StateKeyLaboratoryofMolecularVirologyandGeneticEnginering Beijing100052China

Objective To express human vascular endothelial growth factor (hVEGF-{165}) cDNA in %Pichia pastroris, %purify the expressed product and detect the biological activity of it. Methods By inserting hVEGF-{165} cDNA coding 165 amino acid residues into %Pichia pastoris% expression vector pPIC9K containing AOX1 promoter and the sequences of α secreting signal peptides, a recombinant expression plasmid pPIC9K/hVEGF-{165} was constructed and transformed to yeast host strain KM71, then multiple_copy insert transformants were screened out and cultured in flasks, and hVEGF-{165} was expressed under the induction of 1% methanol. Results SDS_PAGE showed that after being induced with 1% methanol for 4d, the expressed product existed in supernatant in the form of soluble molecule and contained 60% of total protein expressed. Western blot showed good antigenicity and specificity of expressed product. After being purified by Heparin_Sepharose CL6B affinity chromatography, the purity of expressed product reached above 90%. Biological assays proved that the expressed product could stimulate the proliferation of HUVEC. Conclusion hVEGF-{165} was successfully expressed. The study opened up a wide prospect for the application of VEGF-{165} in the prevention and treatment of ischemic heart disease and other tissue ischemic diseases such as secondary arterial occlusion in limbs.

关键词： Base Sequence Blotting Western Chromatography Affinity DNA Primers Electrophoresis Polyacrylamide Gel Endothelial Growth Factors Humans Lymphokines Pichia Plasmids Polymerase Chain Reaction Recombinant Proteins Research Support Non-U.S. Gov't Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors

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Expression of Human Vascular Endothelial Growth Factor Receptor Flt-1 Extracellular Domain 1-3 Loop cDNA in Pichia pastoris, Purification of the Expressed Product and Detection of Its Biological Activity

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Biomedical and Environmental Sciences 2001年第4期14卷 292-301页

作者： mali ZHANGZHI－QING 等 InstituteofMolecularImmunology ThefirstMilitaryMedicalUniversityGuangzhou510515China StateKeyLaboratoryofMolecularVirologyandGeneticEngineering Beijing100052China

Objective To express human vascular endothelial growth factor receptor Flt-1 extracellular domain 1-3 loop cDNA in Pichia. pastroris, and to purify the expressed product and detect its biological activity. Methods By inserting human Flt-1 (1-3 loop) cDNA coding 316 amino acid residues into Pichia pastoris expression vector pPIC9K containing AOX1 promoter and the sequences of α secreting signal peptides, a recombinant expression plasmid pPIC9K/Flt-1 (1-3) was constructed and transformed to yeast host strain GS115, then His + Mut s phenotype transformant was screened out and cultured in flasks, and Flt-1 (1-3) was expressed under the induction of 1% methanol. Results SDS-PAGE showed that after being induced with 1% methanol for 4d, the expressed product existed in supernatant in the form of soluble molecule and contained 60% of total protein expressed. Western blot showed good antigenicity and specificity of expressed product. After being purified by CM-Sepharose FF and Sephacryl S-100 chromatography, the purity of the expressed product reached above 90%. Biological assay proved that the expressed product could bind to hVEGF 165 and inhibit the proliferation of HUVEC stimulated by hVEGF 165. Conclusion Human vascular endothelial growth factor receptor Flt-1 extracellular domain 1-3 loop was successfully expressed. The study lays a foundation for further application of the expressed product in the treatment of vasoformation related diseases, such as tumor and diabetic retinopathy.

关键词： Base Sequence Blotting Western Cells Cultured Cloning Molecular DNA Primers DNA Complementary Electrophoresis Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Phenotype Pichia Plasmids Polymerase Chain Reaction Proto-Oncogene Proteins Receptor Protein-Tyrosine Kinases Recombinant Proteins Research Support Non-U.S. Gov't Vascular Endothelial Growth Factor Receptor-1

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Micro-properties of a type 316 Steel Annealed in Air and Vacuum

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材料热处理学报 2004年第5期25卷 267-270页

作者： LIUGui-min mali-li KURZYDLOWSKIKrzysztofJan AcademyofArmoredForceEngineering BeijingChina WarsawUniversityofTechnology FacultyofMaterialsScienceandEngineeringWarsawPoland

Micro-properties of the oxide scale and near-surface zone of a type 316 steel annealed in air and vacuum have been systematically investigated. Microstructures of the specimens have been examined using optical microscopy and scanning and transmission electron microscopy (TEM). Chemical compositions for the oxide scale and near surface zone were also analysed. The results obtained show that the oxide scale developed in vacuum exhibits a distinct structure from that developed in air. Comparing to the vacuum-developed scale, the air-developed scale possesses a more complicated structure and relatively poor adhesion to the matrix. TEM observations reveal a difference in the dislocation density in the vicinity of the free surface, near-surface zone and the core of the specimens examined.

关键词： 316型钢退火真空热处理微机械性能

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