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LC-MS/MS method for the quantitation of serum tocilizumab in rheumatoid arthritis patients using rapid tryptic digestion without IgG purification

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Journal of Pharmaceutical Analysis 2022年第6期12卷 852-859页

作者： Takashi Mochizuki Kaito shibata Takafumi Naito Kumiko Shimoyama Noriyoshi Ogawa Masato Maekawa Junichi Kawakami Department of Hospital Pharmacy Hamamatsu University School of MedicineHamamatsu431-3192Japan Department of Pharmacy Shinshu University HospitalMatsumotoNagano390-8621Japan Third Division Department of Internal MedicineHamamatsu University School of MedicineHamamatsu431-3192Japan Department of Laboratory Medicine Hamamatsu University School of MedicineHamamatsu431-3192Japan

The quantitation of serum tocilizumab using liquid chromatography tandem-mass spectrometry(LC-MS/MS)method has not been widely applied in clinical settings because of its time-consuming and costly sample *** present study aimed to develop a validated LC-MS/MS method for detecting serum tocilizumab by utilizing immobilized trypsin without an immunoglobulin G purification step and evaluate its applicability in the treatment of rheumatoid arthritis(RA)patients administered intravenously or subcutaneously with *** tocilizumab-derived signature peptide was deciphered using a nano-LC system coupled to a hybrid quadrupole-orbitrap mass *** serum tocilizumab was rapidly digested by immobilized trypsin for 30 *** chromatographic peak of the signature peptide and that of the internal standard were separated from the serum digests for a total run time of 15 *** calibration curve of serum tocilizumab concentration was linear with a range of 2-200 μg/*** intra-and inter-day accuracy and relative standard deviation(RSD)were 90.7%-109.4%and<10%,*** serum tocilizumab concentrations in the RA patients receiving intravenous and subcutaneous injections were 5.8-28.9 and 2.4-63.5 μg/mL,*** serum tocilizumab concentrations using the current method positively correlated with those using the enzyme-linked immunosorbent assay,although a systematic error was observed between these *** conclusion,a validated LC-MS/MS method with minimal sample pretreatments for monitoring serum tocilizumab concentrations in RA patients was developed.

关键词： Tocilizumab LC-MS/MS Immobilized trypsin IgG purification Proteomics Rheumatoid arthritis

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Development and Future Trends of Traditional Kampo Medicine in Japan

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Chinese Medicine and Culture 2022年第1期5卷 58-64页

作者： Risa shibata Gui-Xiang Chu Xun Lin Jin-Rong Fu Gynaecology Department of TCM Longhua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200032China International Office Shanghai University of Traditional Chinese MedicineShanghai 201203China

Traditional Kampo medicine is based on traditional Chinese medicine,which spread to Japan via the Korean Peninsula in the 5th *** practice of Kampo developed gradually under the influence of local Japanese culture and prospered until the Meiji *** appeared in schools successively,such as the School of Later Developments,the School of Classic Methods,the School of Textual Research,and the Integrated ***,the practice of Kampo gradually declined after the Meiji ***,through the continuous efforts of knowledgeable Japanese Kampo practitioners,the practice of Kampo has entered a new era as an indispensable aspect of contemporary Japanese medicine.

关键词： Medical history History Japan Kampo Traditional Chinese medicine

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Micelle-crosslinked hydrogels with stretchable,self-healing,and selectively adhesive properties:Random copolymers work as dynamic yet self-sorting domains

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聚集体(英文) 2023年第3期4卷 71-83页

作者： Hiroaki Asai Motoki shibata Mikihito Takenaka Shinichi Takata Kosuke Hiroi Makoto Ouchi Takaya Terashima Department of Polymer Chemistry Graduate School of EngineeringKyoto UniversityKatsuraKyotoJapan Office of Society-Academia Collaboration for Innovation Kyoto UniversityYoshida-honmachiKyotoJapan Institute for Chemical Research Kyoto UniversityUjiKyotoJapan RIKEN SPring-8 Center Sayo-choSayo-gunHyogoJapan J-PARC Center Japan Atomic Energy AgencyTokaiIbarakiJapan

The design of crosslinking domains is a vital factor to create functional hydrogels with controlled physical,mechanical,and adhesive *** paper demonstrates versatile synthetic systems of micelle-crosslinked hydrogels with highly stretchable,self-healing,and selectively adhesive *** this,methacrylate-bearing random copolymer micelles are designed as physical and covalent crosslink domains via the self-assembly of amphiphilic random copolymers carrying hydrophilic poly(ethylene glycol)(PEG),hydrophobic butyl or dodecyl groups,and methacrylate-terminal PEG in *** size,aggregation number,and pendant methacrylate number of the micelles are controlled by the composition and degree of *** are efficiently obtained from the free radical polymerization of hydrophilic monomers such as PEG acrylate and acrylamide in the presence of the micelle crosslinkers in *** to the dynamic yet selective chain exchange properties of the micelle domains,the hydrogels are highly stretchable up to over 1000%and show self-healing and selectively adhesive *** self-healing of hydrogels is promoted upon heating due to the fast chain exchange of the micelle domains,whereas hydrogels consisting of micelles with different alkyl groups are never adhesive because of their self-sorting properties.

关键词： adhesion hydrogel random copolymer micelle self-assembly self-healing self-sorting

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Leaf-meridian bio-inspired nanofibrous electronics with uniform distributed microgrid and 3D multi-level structure for wearable applications

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npj Flexible Electronics 2022年第1期6卷 372-381页

作者： Mingxu Wang Li Dong Jiajia Wu Jian Shi Qiang Gao Chunhong Zhu hideaki Morikawa Graduate School of Medicine Science and TechnologyShinshu University3-15-1 TokidaUedaNaganoJapan Institute for Fiber Engineering(IFES) Interdisciplinary Cluster for Cutting Edge Research(ICCER)Shinshu University3-15-1 TokidaUedaNaganoJapan Faculty of Systems Science and Technology Akita Prefectural University84-4 Aza EbinokuchiTsuchiyaYurihonjoAkitaJapan School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhouChina Faculty of Textile Science and Technology Shinshu University3-15-1 TokidaUedaNaganoJapan

The interface between the active electronic and its osculatory target dominates the sensing response of high-sensitivity ***,the interface properties are difficult to be adjusted and preserved owing to the limited strategies for surface *** this work,inspired by nature frond leaf,a spatial multi-level nanofibrous membrane with grid-like microstructure of uniform distribution was fabricated,in which carboxylated carbon nanotubes(CCNTs)/poly(3,4-ethylenedioxythiophene)(PEDOT)was modified onto the surface of grid-like polyurethane(PU)nanofiber via the combination of metal mesh template,in situ polymerization and ultrasonic *** membrane enables a pressure sensor with high sensitivities(5.13 kPa−1),fast response/recovery time(80 ms and 120 ms),and ultralow detection limit of 1 *** addition,as a scalable and integrable platform,we also demonstrate its multifunctional applications for electro-thermal conversion and energy *** these results indicate the proposed nanofibrous membrane may potentially be applied to next-generation wearable devices.

关键词： treatment. template polyurethane

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Recent progress of laboratory astrophysics with intense lasers

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High Power Laser Science and Engineering 2021年第4期9卷 7-40页

作者： hideaki Takabe Yasuhiro Kuramitsu School of Engineering Osaka UniversitySuitaOsaka 565-0871Japan Institute of Laser Engineering Osaka UniversitySuitaOsaka 565-0871Japan Institute of Radiation Physics Helmholtz Zentrum Dresden Rossendorf01328 DresdenGermany

Thanks to a rapid progress of high-power lasers since the birth of laser by *** in 1960,intense lasers have been developed mainly for studying the scientific feasibility of laser *** confinement fusion with an intense laser has attracted attention as a new future energy source after two oil crises in the 1970s and *** the beginning,the most challenging physics is known to be the hydrodynamic instability to realize the spherical implosion to achieve more than 1000 times the solid *** studies have been performed theoretically and experimentally on the hydrodynamic instability and resultant turbulent mixing of compressible *** such activities in the laboratory,the explosion of supernova SN1987A was observed in the sky on 23 February *** X-ray satellites have revealed that the hydrodynamic instability is a key issue to understand the physics of supernova *** collaboration between laser plasma researchers and astrophysicists,the laboratory astrophysics with intense lasers was proposed and promoted around the end of the *** original subject was mainly related to hydrodynamic ***,after two decades of laboratory astrophysics research,we can now find a diversity of research *** has been demonstrated theoretically and experimentally that a variety of nonlinear physics of collisionless plasmas can be studied in laser ablation plasmas in the last *** the present paper,we shed light on the recent 10 topics studied intensively in laboratory experiments.A brief review is given by citing recent ***,modeling cosmic-ray acceleration with lasers is reviewed in a following session as a special topic to be the future main topic in laboratory astrophysics research.

关键词： collisionless shock compressible hydrodynamics cosmic rays equation of state high-energy-density plasmas intense laser magnetic turbulence opacity experiment particle accelerations relativistic plasmas turbulent mixing wakefield acceleration Weibel instability

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Implications of Transient Receptor Potential Cation Channels in Migraine Pathophysiology

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Neuroscience Bulletin 2021年第1期37卷 103-116页

作者： Mamoru shibata Chunhua Tang Department of Neurology Keio University School of MedicineTokyo 160-8582Japan Department of Neurology Tokyo Dental College Ichikawa General HospitalChiba 272-8513Japan Department of Neurology and Center for Clinical Neuroscience Daping HospitalThird Military Medical UniversityChongqing 400042China

Migraine is a common and debilitating headache disorder. Although its pathogenesis remains elusive,abnormal trigeminal and central nervous system activity is likely to play an important role. Transient receptor potential(TRP) channels, which transduce noxious stimuli into pain signals, are expressed in trigeminal ganglion neurons and brain regions closely associated with the pathophysiology of migraine. In the trigeminal ganglion,TRP channels co-localize with calcitonin gene-related peptide, a neuropeptide crucially implicated in migraine pathophysiology. Many preclinical and clinical data support the roles of TRP channels in migraine. In particular,activation of TRP cation channel V1 has been shown to regulate calcitonin gene-related peptide release from trigeminal nerves. Intriguingly, several effective antimigraine therapies, including botulinum neurotoxin type A, affect the functions of TRP cation channels. Here, we discuss currently available data regarding the roles of major TRP cation channels in the pathophysiology of migraine and the therapeutic applicability thereof.

关键词： Migraine TRPV1 TRPM8 TRPA1 TRPV4 Calcitonin gene-related peptide Trigeminal ganglion Neurogenic inflammation

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Investigating drivers of active nitrification in organic horizons of tropical forest soils

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Soil Ecology Letters 2023年第3期5卷 89-99页

作者： Shinichi Watanabe Makoto shibata Yoshiko Kosugi Lion Marryanna Keitaro Fukushima Arief Hartono Shinya Funakawa Graduate School of Global Environmental Studies Kyoto UniversityKitashirakawa Oiwake-choSakyo-kuKyoto606-8502Japan Graduate School of Agriculture Kyoto UniversityKitashirakawa Oiwake-choSakyo-kuKyoto606-8502Japan Forest Research Institute Malaysia 52109 KepongSelangorMalaysia Center for Ecological Research Kyoto University509-3Hirano 2-chomeOtsu-shiShiga520-2113Japan Faculty of Agriculture Bogor Agricultural UniversityJalan MerantiKampus IPB Darmaga 16680BogorIndonesia

High nitrate leaching has been observed from the O horizons of some tropical forests;however,the drivers of high nitrate production(active nitrification)in these O horizons have not yet been *** study investigated the drivers of active nitrification in the O horizon of tropical forest soils by focusing on two of the most widely recognized controlling factors of nitrification,total N,and *** collected mineral and O horizons from eight tropical forests in Cameroon,Indonesia,and Malaysia and measured gross nitrification *** O horizons showed significantly higher gross nitrification rates than mineral horizons,indicating that these O horizons have a high potential for *** nitrification rates in the O horizons were positively correlated with both total N and pH,and the chemical properties(e.g.,total content of N,P,and base cations)were *** correlations suggested that the underlying driver of nitrification in the O horizon was nutrient richness in the *** also indicated a threshold of gross nitrification rates around pH values of 5.5–*** elucidate that active nitrification and subsequent high nitrate leaching from the O horizon could be driven by nutrient-rich litter,possibly derived from soil fertility and tree species.

关键词： nitrogen dynamics gross nitrification rate organic horizon forest floor acidic soil leguminous trees

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Erratum:Cooperative distributed state estimation:resilient topologies against smart spoofers

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Control Theory and Technology 2023年第4期21卷 613-614页

作者： Mostafa Safi Seyed Mehran Dibaji hideaki Ishii Amirkabir University of Technology HafezTehran424Iran Department of Mechanical Engineering Massachusetts Institute of TechnologyCambridgeMAUSA Department of Computer Science Tokyo Institute of TechnologyYokohamaJapan

In the original publication of this article[1],the second author Seyed Mehran Dibaji and the third author hideaki Ishii were mentioned in the‘Acknowledgments'*** publication,authors agreed the contribution should hav... 详细信息

关键词： smart mentioned ledgment

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PROFESSOR BRITTON CHANCE AND THE EARLY TIMES HE LIVED

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Journal of Innovative Optical Health Sciences 2011年第3期4卷 215-219页

作者： hideaki KOIZUMI Hitachi Ltd.

*** Professor Britton Chance passed away on November 16,*** on July 24,1913,he was 97 years old when he died.I was honored to have had the opportunity to meet Professor Chance and have friendly conversation at the ear... 详细信息

关键词： Chance passed conversation

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Chrono-exercise: Time-of-day-dependent physiological responses to exercise

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运动医学与健康科学(英文) 2023年第1期5卷 50-58页

作者： Hyeon-Ki Kim Zsolt Radak Masaki Takahashi Takayuki Inami Shigenobu shibata Research Center for Molecular Exercise Science University of Physical EducationBudapestHungary Institute of Physical Education Keio UniversityYokohamaJapan Chrono-nutrition Research Center Waseda UniversityTokyoJapan Institute for Liberal Arts Tokyo Institute of TechnologyTokyoJapan Faculty of Science and Engineering Waseda UniversityJapan

Exercise is an effective strategy to prevent and improve obesity and related metabolic *** increases the metabolic demand in the *** many of the metabolic health benefits of exercise depend on skeletal muscle adaptations,exercise exerts many of its metabolic effects through the liver,adipose tissue,and ***,exercise is the physiological state in which inter-organ signaling is most *** contrast,circadian rhythms in mammals are associated with the regulation of several physiological and biological functions,including body temperature,sleep-wake cycle,physical activity,hormone secretion,and metabolism,which are controlled by clock *** and lipid tolerance reportedly exhibit diurnal variations,being lower in the evening than in the ***,the effects of exercise on substrate metabolism at different times of the day may *** this review,the importance of exercise timing considerations will be outlined,incorporating a chrono-exercise perspective.

关键词： Chrono-exercise Exercise timing Inter-organ communication Circadian rhythm Energy metabolism

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