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Ng Ago: an exciting new tool for genome editing

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Science Bulletin 2016年第14期61卷 1074-1075页

作者： Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences

Site-directed DNA endonucleases are powerful tools for genome editing.When introduced into cells,these systems can bind to a target DNA sequence in the genome and create a DNA double-strand break(DSB),the repair

关键词：编辑工具基因组 DNA双链断裂 DNA序列免疫系统核酸内切酶兴奋 RNA

Gene editing in T cell therapy

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Journal of Genetics and Genomics 2017年第9期44卷 415-422页

作者： Yongping Zhang Wei Mu Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Department of Hematology Aerospace Center Hospital Aerospace Clinical Medical College Peking University University of Chinese Academy of Sciences

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise. Gene editing holds tremendous potential for further improvements of T cell therapy. Here we review the applications of gene editing in various T cell therapies, focusing on antiviral strategies and cancer immunotherapies, and discuss the challenges and future prospects.

关键词： Gene editing CRISPR-Cas9 T cells

Recent advances in CRISPR research

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Protein & Cell 2020年第11期11卷 786-791页

作者： Baohui Chen Yuyu Niu Haoyi Wang Kejian wang Hui Yang Wei Li Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310058China Yunnan Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Research Center for Brain Science and Brain-Inspired IntelligenceShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China

The clustered regularly interspaced short palindromic repeats(CRISPR)technology has revolutionized life sciences and developed rapidly.Here,we highlight the recent advances in development and application of CRISPR technologies,including the discovery of novel CRISPR systems,CRISPR base editing and imaging,and the applications of CRISPR in plant breeding,animal breeding,disease modeling and biotherapy.

关键词： CRISPR breeding advances

Extended pluripotent stem cells facilitate mouse model generation

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Protein & Cell 2019年第1期10卷 5-7页

作者： Guanghai Xiang Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Mouse embryonic stem(mES)cells,established in 1981(Evans and Kaufman,1981;Martin,1981),were derived from the inner cell mass(ICM)of blastocysts and can be expanded in vitro for many passages,maintaining normal karyotype and differentiation potential.Upon introduction into blastocysts,mES cells can differentiate into all three germ layers,contributing to all the somatic lineages and germline.

关键词： stem Martin cells

Temperature effect on CRISPR-Cas9 mediated genome editing

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Journal of Genetics and Genomics 2017年第4期44卷 199-205页

作者： Guanghai Xiang Xingying Zhang Chenrui An Chen Cheng Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 700049 China University of Science and Technology of China Hefei 230026 China

Zinc-finger nuclease（ZFN）, transcription activator-like effector nuclease（TALEN）, and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9（CRISPR-Cas9） are the most commonly used genome editing tools. Previous studies demonstrated that hypothermia treatment increased the mutation rates induced by ZFNs and TALENs in mammalian cells. Here, we characterize the effect of different culture temperatures on CRISPR-Cas9 mediated genome editing and find that the genome editing efficiency of CRISPR-Cas9 is significantly hampered by hypothermia treatment, unlike ZFN and TALEN. In addition, hyperthermia culture condition enhances genome editing by CRISPR-Cas9 in some cell lines, due to the higher enzyme activity and sg RNA expression level at higher temperature. Our study has implications on CRISPR-Cas9 applications in a broad spectrum of species, many of which do not live at 37 C.

关键词： CRISPR-Cas9 Hyperthermia Genome editing Mammalian cells

Glucocorticoid receptor modulates myeloid-derived suppressor cell function via mitochondrial metabolism in immune thrombocytopenia

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Cellular & Molecular Immunology 2022年第7期19卷 764-776页

作者： Yu Hou Jie Xie Shuwen wang Daqi Li Lingjun wang Haoyi Wang Xiaofei Ni Shaoqiu Leng Guosheng Li Ming Hou Jun Peng Department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Shandong Provincial Key Laboratory of Immunohematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Jinan Central Hospital Cheeloo College of MedicineShandong UniversityJinanChina Leading Research Group of Scientific Innovation Department of Science and Technology of Shandong ProvinceQilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Advanced Medical Research Institute Shandong UniversityJinanChina

Myeloid-derived suppressor cells(MDSCs)are a heterogeneous population of immature cells and natural inhibitors of adaptive immunity.Intracellular metabolic changes in MDSCs exert a direct immunological influence on their suppressive activity.Our previous study demonstrated that high-dose dexamethasone(HD-DXM)corrected the functional impairment of MDSCs in immune thrombocytopenia(ITP);however,the MDSC population was not restored in nonresponders,and the mechanism remained unclear.In this study,altered mitochondrial physiology and reduced mitochondrial gene transcription were detected in MDSCs from HD-DXM nonresponders,accompanied by decreased levels of carnitine palmitoyltransferase-1(CPT-1),a rate-limiting enzyme in fatty acid oxidation(FAO).Blockade of FAO with a CPT-1 inhibitor abolished the immunosuppressive function of MDSCs in HD-DXM responders.We also report that MDSCs from ITP patients had lower expression of the glucocorticoid receptor(GR),which can translocate into mitochondria to regulate the transcription of mitochondrial DNA(mtDNA)as well as the level of oxidative phosphorylation.It was confirmed that the expression of CPT-1 and mtDNA-encoded genes was downregulated in GR-siRNA-treated murine MDSCs.Finally,by establishing murine models of active and passive ITP via adoptive transfer of DXM-modulated MDSCs,we confirmed that GR-silenced MDSCs failed to alleviate thrombocytopenia in mice with ITP.In conclusion,our study indicated that impaired aerobic metabolism in MDSCs participates in the pathogenesis of glucocorticoid resistance in ITP and that intact control of MDSC metabolism by GR contributes to the homeostatic regulation of immunosuppressive cell function.

关键词： immune thrombocytopenia myeloid-derived suppressor cell HD-DXM glucocorticoid receptor

Three-dimensional architectures constructed using two-dimensional nanosheets

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Science China Chemistry 2015年第12期58卷 1792-1799页

作者： haoyi Li Xun wang Department of Chemistry Tsinghua University

Two-dimensional(2D) nanomaterials such as transition metal dichalcogenides(TMDs) and graphene have attracted extensive interest as emergent materials, owing to their excellent properties that favor their future use in electronic devices, catalysis, optics, and biological- or energy-relevant areas. However, 2D nanosheets tend to easily restack and condense, which weakens their performance in many of these applications. Assembling these 2D nanosheets as building blocks for three-dimensional(3D) architectures not only maintains the intrinsic performances of the 2D nanostructures but also synergistically makes use of the advantages of the 3D microstructures to improve the overall material properties. In this critical review, we will highlight recent developments of sundry 2D nanosheet-assembled 3D architectures, including their design, synthesis, and potential applications. Their controllable syntheses, novel structures, and potential applications will be systematically explained, analyzed, and summarized. In the end, we will offer some perspective on the challenges facing future advancement of this field.

关键词： 3D architectures 2D nanosheets restack assembly

Multivalent metal-sulfur batteries for green and cost-effective energy storage:Current status and challenges

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Journal of Energy Chemistry 2022年第1期31卷 144-165,I0005页

作者： Yue Yang haoyi Yang Xinran wang Ying Bai Chuan Wu Beijing Key Laboratory of Environmental Science and Engineering School of Materials Science&EngineeringBeijing Institute of TechnologyBeijing 100081China Collaborative Innovation Center of Electric Vehicles in Beijing Beijing 100081China

Multivalent metal-sulfur(M-S,where M=Mg,Al,Ca,Zn,Fe,etc.)batteries offer unique opportunities to achieve high specific capacity,elemental abundancy and cost-effectiveness beyond lithium-ion batteries(LIBs).However,the slow diffusion of multivalent-metal ions and the shuttle of soluble polysulfide result in impoverished reversible capacity and limited cycle performance of M-S(Mg-S,Al-S,Ca-S,Zn-S,Fe-S,etc.)batteries.It is a necessity to optimize the electrochemical performance,while deepening the understanding of the unique electrochemical reaction mechanism,such as the intrinsic multi-electron reaction process,polysulfides dissoluti on and the in stability of metal an odes.To solve these problems,we have summarized the state-of-the-art progress of current M-S batteries,and sorted out the existing challen ges for different multivalent M-S batteries according to sulfur cathode,electrolytes,metallic an ode and current collectors/separators,respectively.In this literature,we have surveyed and exemplified the strategies developed for better M-S batteries to strengthen the application of green,cost-effective and high energy density M-S batteries.

关键词： Multivale nt metal-sulfur batteries Cost-effectiveness Green energy storage Shuttle effect Electrolyte

5'capped and 3'polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system

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Protein & Cell 2019年第3期10卷 223-228页

作者： Wei Mu Yongping Zhang Xutong Xue Lei Liu Xiaofei Wei Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Hematology Aerospace Center HospitalAerospace Clinical Medical CollegePeking UniversityBeijing 100049China Hebei University Baoding 071002China Beijing Cord Blood Bank Beijing 100044China

Dear Editor,The clustered regularly interspaced short palindromic repeats(CRISPR)-associated(Cas)system is an adaptive immune system in a variety of bacteria and archaea(Terns and Terns,2011).The most commonly used Streptococcus pyogenes type II CRISPR-Cas9 system consists of Cas9 nuclease and two short RNAs,crRNA and tracrRNA,which can be linked together forming one chimeric single guide RNA(sgRNA)(Jinek et al.,2012).

关键词： Editor CRISPR Cas