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Bonding Efficacy of a Self-adhesive Resin Cement to Enamel and Dentin

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Journal of Wuhan University of Technology(Materials Science) 2014年第6期29卷 1307-1312页

作者：王林虎 xu haixing LI Songyang 施斌 LI Rong YE Mingfu YANG Jing Department of Implantology Hospital of Stomatology Wuhan University Department of Pharmaceutical Engineering School of Chemistry Chemical Engineering and Life Sciences Wuhan University of Technology Department of Biology Hongkong Baptist University

With the objective to evaluate the bonding efficacy of a new self-adhesive resin cement（RelyX Unicem, 3M ESPE） to enamel and dentin using a shear bonding strengths test with or without acid etching pretreatment, flat buccal dentin surface and mesial/distal enamel surface were made using a high-speed diamond bur. Copper rings were luted using Rely X Unicem（RU; 3M ESPE）, Panavia F（PF; Kuraray） or Vitique（VI; DMG）. For RU, the shear bonding strengths using GL（Gluma Etch, Heraeus） acid etching pretreatment were also tested. The teeth were placed into copper rings（inner diameter： 16 mm, height： 4mm） and embedded in methylmethacrylate resin. The specimens were stored for 24 h in distilled water at 37 ℃ prior to shear bonding strengths testing. In addition, bond failures were examined by optical microscope and categorized as 4 models such as different adhesive, cohesive, or mixed. Shear bonding strengths were calculated by dividing the maximum debonding force over the cross sectional area of each specimen. The Kruskal-Wallis test was used to determine pairwise statistical differences（P 〈 0.05） in SBS between the experimental groups. For dentin bonding strength, statistical analysis showed that there was no significant difference among RU（12.84 MPa）, PF（14.93 MPa） and VI（11.03 MP）; and the bonding strengths of them were higher than RU with acid etching pretreatment（9.12 MP）. When bonded to enamel, PF（17.99 MP） and VI（17.58 MP） scored significantly higher than RU *** use of self-adhesive cement Rely X Unicem can obtain the bonding strengths to dentin similar to traditional resin cements. Phosphoric acid etching can improve the bonding strengths of the selfadhesive resin cement to enamel, but was negative for dentin.

关键词： RelyX Unicem dentin enamel shear bond strength

Synthesis and Evaluation of Novel Chitosan Derivatives for Gene Delivery

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Journal of Wuhan University of Technology(Materials Science) 2015年第4期30卷 852-858页

作者：付芳 LIU Xiong 吕波 QU Guoyi ZHENG Hua HUANG Zhijun xu Peihu xu haixing YIN Yihua LIU Xia ZHANG xueqiong School of Chemistry Chemical Engineering and Life Sciences Wuhan University of Technology Luo Tian Wan Mizhai Hospital

A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293T and HeLa cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors.

关键词： gene vector transfection efficiency chitosan non-viral vector

Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres

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Journal of Wuhan University of Technology(Materials Science) 2013年第6期28卷 1231-1235页

作者： xu haixing ZHANG Yu NIU Xiaoqian WANG Ling CHEN Hui ZHANG Xi TANG Qiuhan HUANG Zhijun LIU Hui xu Peihu Department of Pharmaceutical Engineering School of Chemical EngineeringWuhan University of Technology Engineering Technology Center of Hubei traditional Chinese medicine preparation

Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this approach was evaluated in terms of drug loading content （DLC）, encapsulation efficiency （EE） and delivery properties in vitro, determined by high performance liquid chromatograph （HPLC）. The microspheres showed good DLC values of 11.8%, as well as good EE values of 79.4%. The in vitro drug release study was carried out in phosphate buffer solution （PBS） simulated body fluid, at 37 ~C with pH=7.4. The release profiles showed an initial fast release rate, which decreased as time progressed and about 84 % had been released after 48 h. The experimental results indicated that the prepared magnetic microspheres may be useful for potential applications of MER for magnetically targeted chemotherapy.

关键词： mercaptopurine magnetic microspheres drug loading content encapsulation efficiency

PDLLA/β-TCP/HA/CHS/NGF Sustained-release Conduits for Peripheral Nerve Regeneration

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Journal of Wuhan University of Technology(Materials Science) 2021年第4期36卷 600-606页

作者： YAN Xiumei WANG Jing HE Qundi xu haixing TAO Junyan KORAL Kelly LI Kebi xu Jingyi WEN Jing HUANG Zhijun xu Peihu Department of Pharmaceutical Engineering School of ChemistryChemical Engineering and Life ScienceWuhan University of TechnologyWuhan 430070China China Resources Sanjiu Medical&Pharmaceutical Co. Ltd.Shenzhen 518029China Wuhan Mafangshan Middle School Wuhan 430070China Department of Pathology University of Pittsburgh.PittsburghPAUSA

Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to *** with NGCs made of single material,composite NGCs have a greater development *** previous research has confirmed that poly(D,L-lactic acid)/β-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/β-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties,which can slowly release NGF and support cell adhesion and *** this study,PDLLA/β-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs in *** improvements in nerve regeneration were observed after using the PDLLA/β-TCP/HA/CHS/NGF NGCs based on gross post-operation observation,triceps wet weight analysis and nerve histological *** vivo studies illustrate that the PDLLA/β-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration,and the effect is similar to that of autograft.

关键词： sustained-release composite nerve conduits peripheral nerve regeneration

Preparation and Characterization of Poly Lactic Acid/Graphene Oxide/Nerve Growth Factor Scaffold with Electrical Stimulation for Peripheral Nerve Regeneration in vitro

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Journal of Wuhan University of Technology(Materials Science) 2020年第6期35卷 1149-1161页

作者： xu haixing LI Rui LI Yiping HE Qundi YAN Xiumei SHU Tao YANG Haixia LÜ Yifei LI Zheng xu Runtian XIONG Chengjie xu Peihu Department of Pharmaceutical Engineering School of ChemistryChemical Engineering and Life ScienceWuhan University of TechnologyWuhan 430070China Wuhan Mafangshan Middle School Wuhan 430070China Orthopaedic Department General Hospital of Central Theater Command of PLAWuhan 430070China

A novel conductive drug-loading system was prepared by using an improved emulsion electrostatic spinning method which contained polylactic acid (PLA),graphene oxide (GO),and nerve growth factor (NGF) coated with bovine serum albumin (BSA) ***,the structure,mechanical properties,morphology and electrical conductivity of PLA/GO electro spun fiber membranes with different GO ratios were ***/GO scaffolds can exhibit superior porosity,hydrophilic and biomechanical properties when the GO incorporation rate is 0.5%.The addition of GO in the PLA/GO electro spun fiber membranes can also create appropriate pH environment for the repair of injured nerve when the GO incorporation rate is above 0.5%.Secondly,PLA/GO/BSA/Genipin/NGF particles (with a ratio of BSA/NGF=3:1) prepared by modified emulsion electro spinning method will release more NGF than PLA/GO/NGF *** addition,PLA/0.5%GO/NGF scaffold can maintain its structure stability for at least 8 weeks observed by scanning electron microscope (SEM).Moreover,the degradation of PLA/0.5%GO/NGF scaffold is consistent with its weight ***,in vitro assay confirmes that PLA/GO composite scaffold exhibits low cytotoxicity to RSC96 *** results have demonstrated that PLA/0.5%GO/NGF sustained-release drug sustained-release system with appropriate electrical stimulation (ES) can promote PC12 cell proliferation,and it can maintain its differentiation capability for at least 3 *** conclusion,PLA/0.5%GO/NGF sustained-release drug sustained-release system can maintain its biological activity for at least 3 weeks and promote cell proliferation with appropriate ES.

关键词： poly lactic acid graphene oxide nerve growth factor electrical stimulation peripheral nerve regeneration