Preparation and Characterization of Poly Lactic Acid/Graphene Oxide/Nerve Growth Factor Scaffold with Electrical Stimulation for Peripheral Nerve Regeneration in vitro

作     者：XU Haixing LI Rui LI Yiping HE Qundi YAN Xiumei SHU Tao YANG Haixia LÜ Yifei LI Zheng XU Runtian XIONG Chengjie XU Peihu 徐海星;LI Rui;LI Yiping;HE Qundi;YAN Xiumei;SHU Tao;YANG Haixia;LÜ Yifei;LI Zheng;XU Runtian;熊承杰;许沛虎

作者机构：Department of Pharmaceutical EngineeringSchool of ChemistryChemical Engineering and Life ScienceWuhan University of TechnologyWuhan 430070China Wuhan Mafangshan Middle SchoolWuhan 430070China Orthopaedic DepartmentGeneral Hospital of Central Theater Command of PLAWuhan 430070China 

出 版 物：《Journal of Wuhan University of Technology(Materials Science)》 (武汉理工大学学报（材料科学英文版）)

年 卷 期：2020年第35卷第6期

页      面：1149-1161页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Funded by the National Natural Science Foundation of China(No.51572206) the Wuhan Huanghe Excellence Plan Natural Science Foundation of Hubei(2018CFB487) the National Innovation and Entrepreneurship Training Program for College Students(Nos.202010497028,202010497030,and 202010497062) 

主　　题：poly lactic acid graphene oxide nerve growth factor electrical stimulation peripheral nerve regeneration 

摘      要：A novel conductive drug-loading system was prepared by using an improved emulsion electrostatic spinning method which contained polylactic acid (PLA),graphene oxide (GO),and nerve growth factor (NGF) coated with bovine serum albumin (BSA) ***,the structure,mechanical properties,morphology and electrical conductivity of PLA/GO electro spun fiber membranes with different GO ratios were ***/GO scaffolds can exhibit superior porosity,hydrophilic and biomechanical properties when the GO incorporation rate is 0.5%.The addition of GO in the PLA/GO electro spun fiber membranes can also create appropriate pH environment for the repair of injured nerve when the GO incorporation rate is above 0.5%.Secondly,PLA/GO/BSA/Genipin/NGF particles (with a ratio of BSA/NGF=3:1) prepared by modified emulsion electro spinning method will release more NGF than PLA/GO/NGF *** addition,PLA/0.5%GO/NGF scaffold can maintain its structure stability for at least 8 weeks observed by scanning electron microscope (SEM).Moreover,the degradation of PLA/0.5%GO/NGF scaffold is consistent with its weight ***,in vitro assay confirmes that PLA/GO composite scaffold exhibits low cytotoxicity to RSC96 *** results have demonstrated that PLA/0.5%GO/NGF sustained-release drug sustained-release system with appropriate electrical stimulation (ES) can promote PC12 cell proliferation,and it can maintain its differentiation capability for at least 3 *** conclusion,PLA/0.5%GO/NGF sustained-release drug sustained-release system can maintain its biological activity for at least 3 weeks and promote cell proliferation with appropriate ES.