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Quality Standard of Tibetan Medicine Nardostachys jatamansi Herba Based on"An Integrated Plant but Multi-purpose"

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Medicinal Plant 2024年第3期15卷 16-22页

作者： hairong zhong Yuebu HAILAI Shaoshan ZHANG Wenbing LI Yuan LIU College of Pharmacy Sichuan College of Traditional Chinese MedicineMianyang 621000China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory Chengdu 610225China Key Laboratory of Protection and Utilization of Ethnic Medicinal Resources on Qinghai-Tibet Plateau State Ethnic Affairs CommissionChengdu 610225China Southwest Minzu University Chengdu 610225China

[Objectives]To establish the quality standard of Nardostachys jatamansi Herba.[Methods]The characters and microscopical identification of *** Herba were carried *** contents of moisture,total ash,acid-insoluble ash and extract were determined according to the relevant methods of the Chinese Pharmacopoeia(2020 edition).Using chlorogenic acid and 3,5-O-dicaffeoylquinic acid as quality control indexes,TLC and HPLC methods were established for qualitative and quantitative determination,and HPLC fingerprints were established.[Results]The characteristics of character identification,microscopic identification and thin layer identification were *** moisture content ranged from 2.7%to 7.8%,with an average value of 5.4%.The total ash content ranged from 6.7%to 16.2%,with an average of 11.0%.The acid-insoluble ash content ranged from 0.7%to 8.5%,with an average of 3.6%.Extractives content ranged from 20.9%to 34.4%,with an average of 29.7%.Chlorogenic acid content was between 0.45%and 1.30%,with an average value of 0.77%.The content of 3,5-O-dicaffeoylquinic acid ranged from 0.18%to 0.58%,with an average of 0.31%.The similarity of each batch was between 0.930 and 0.994,indicating that the quality of medicinal materials from different producing areas was stable.[Conclusions]The quality standard of *** Herba was established,which could provide quality control basis for rational,comprehensive and efficient utilization of *** *** and clinical use.

关键词： Nardostachys jatamansi Herba Chlorogenic acid 3 5-O-dicaffeoylquinic acid Fingerprint Quality standard Resource utilization

Inhibitiont of Obazema on Proliferation of Gastric Cancer BGC-823 Cells and Its Mechanism

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Medicinal Plant 2019年第6期10卷 42-46,51页

作者： Ying LI Risha WEIZE hairong zhong Jixiu SHEN Yisong LI Yuan LIU College of Pharmacy Southwest Minzu UniversityChengdu 610041China Ethnic Medicine Institute Southwest Minzu UniversityChengdu 610041China

[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentrations of drug-containing serum(0.316%,0.158%and 0.079%)for 0,48,72 and 96 h,***,the proliferation of the cells was detected with CCK-8 method,and the expression of related proteins,B lymphocytoma-2(Bcl-2),phosphorylated protein kinase B(p-Akt),protein kinase B(Akt)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH),was detected using Western blotting.[Results]The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.)*** ***(CH)and *** Lévl.(S)(P<0.01),in dose-dependent and time-dependent *** inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration;the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01);different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells;the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05,P<0.01),in a certain dose-dependent manner;at the same dose,the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05,P<0.01);administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05,P<0.01).[Conclusions]Obazema has the capacity to inhibit the proliferation of BGC-823 *** mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2,and GAPDH may be the target gene of its anti-tumor *** inhibiting effect of CH on BGC-823 cells was more significantly than that of S.

关键词： Yi Medicine Obazema BGC-823 cell Boenninghausenia albiflora(Hook.)Reichb.ex Meisn.var.albiflora Boenninghausenia sessilicarpa Lévl

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Medicinal Plant 2019年第2期10卷 73-78,84页

作者： Nannan LONG Risha WEIZE hairong zhong Xiaoxia CAI Yuan LIU Ying LI School of Pharmacy Southwest Minzu University Sintanovo Biotech(Chengdu) Co. Ltd.

[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different solvents(petroleum ether, chloroform, ethyl acetate and methanol) were used to extract the chemical components of different polar regions of B. sessilicarpa and B. albiflora. UV-visible spectrophotometry(second derivative method) was used to collect the fingerprints of different solvent extracts, and common and variant peak peak ratios were analyzed for the absorption peak data. [Results] The common peak ratio and variant peak ratio data of ground parts of B. albiflora and B. sessilicarpa was S2∶S5(46.2(54.2, 62.5)), compared with the data of other groups, the common peak ratio was the largest, thus the components of ground parts of B. albiflora and B. sessilicarpa were closest and had the largest similarities; the common peak ratio and variant peak ratio data of the components extracted by chloroform from B. albiflora and B. sessilicarpa was Y2∶Y6(54.2(38.5, 46.2)), compared with the data of other groups, the variant peak ratio was the smallest, thus, the chemical components near the chloroform polarity of two kinds of medicinal materials had the largest similarities and smallest differences. [Conclusions] This method is simple and easy to operate, and the ultraviolet fingerprint data of four different polar organic solvent extracts are used for comprehensive analysis, and the results have high specificity and high accuracy. Besides, there are certain similarities and also differences between the chemical components of B. sessilicarpa and B. albiflora. It is expected to provide a new evaluation method for the variety quality of B. sessilicarpa and B. albiflora.

关键词： Boenninghausenia albiflora (Hook.) Meiss Boenninghausenia sessilicarpa Levl. Ultraviolet fingerprint Common peak ratio Variant peak ratio

Identification of Original Plants, Traits, Microstructure and Powder of Leontopodium franchetii Beauv.

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Medicinal Plant 2019年第1期10卷 57-61页

作者： Xiaoxia CAI Xiaoyan ZHAO Xuexue LI hairong zhong Yunzhang XU Yuan LIU College of Pharmacy Southwest Minzu University State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of Sciences Ethnic Medicine Institute Southwest Minzu University

[Objectives] This study aimed to provide experimental data for the identification and application of Leontopodium franchetii Beauv. [Methods] The original plants, traits, microstructure and powder of L. franchetii Beauv. were identified. [Results] The identification characteristics of L. franchetii Beauv. in the original plants, traits and microstructure were obvious. [Conclusions] This study can provide reference for the identification of original plants and herbs and the development of quality standards of L. franchetii Beauv. and provide a basis for further research and development.

关键词： Leontopodium franchetii Beauv. Identification Original plants Traits

Comprehensive Evaluation on Impacts of Diseases on Quality of Paris polyphylla var. chinensis Based on Ultraviolet and Infrared Spectra

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Medicinal Plant 2019年第3期10卷 63-65,68页

作者： Yongxia KE Jixiu SHEN hairong zhong Risha WEIZE Yunzhang XU Yuan LIU College of Pharmacy Southwest Minzu UniversityChengdu610041China State Key Laboratory of Systematic and Evolutionary Botany Institute of Botanythe Chinese Academy of SciencesBeijing100093China Ethnic Medicine Institute Southwest Minzu UniversityChengdu610041China

[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis.

关键词： Paris polyphylla var. chinensis Ultraviolet Infrared Total saponins Quality

Determination of Inorganic Elements in Roots, Stems and Leaves of Arctium lappa L. by ICP-OES

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Medicinal Plant 2019年第1期10卷 34-36页

作者： hairong zhong Chunlin LIU Jixiu SHEN Risha WEIZE Jingxia WANG Yuan LIU College of Pharmacy Southwest Minzu University College of Chemistry and Chemical Engineering Shaanxi University of Science and Technology Sichuan Technology & Business College Ethnic Medicine Institute Southwest Minzu University

[Objectives] To determine the content of 20 inorganic elements in 18 samples of roots, stems and leaves from of Arctium lappa L. produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma emission spectrometry(ICP-OES) were used. [Results] 20 kinds of inorganic elements in the samples of A. lappa L. roots contained rich elements essential for human beings such as K, Ca, Na, P and trace elements Cu, Fe, Zn; heavy metals Pb, As, Cu, Cd in A. lappa L. samples did not exceed the limit, and Hg was not detected in all 12 samples; heavy metals in A. lappa L. roots harvested in 12 months did not exceed the limit. [Conclusions] Compared with the roots of A. lappa L., the inorganic elements in the stems and leaves of A. lappa L. were relatively less. The samples collected from Shuimo Town in Wenchuan County of Aba Prefecture with an altitude of 920 m showed little difference from samples collected from Qiongxi Town in Hongyuan County of Aba Prefecture with an altitude of 3 505 m in 20 kinds of inorganic elements.

关键词： Arctium lappa L. Inorganic elements Inductively coupled plasma optical Heavy metals

大规模联邦仿真中实体迁移及其时间同步研究(一)——实体迁移协议与实现

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计算机研究与发展 2005年第4期42卷 711-715页

作者：刘晓建钟海荣金士尧国防科学技术大学计算机学院并行与分布处理国家重点实验室长沙410073

实体迁移技术有利于改善大规模分布式仿真系统的性能.首先分析了新一代仿真体系结构HLARTI对实体迁移支持上的不足和现有HLA兼容型仿真系统中实体迁移实现方法的缺陷;然后提出了在HLARTI下进行实体迁移的协议,设计了实体最新状态传输机... 详细信息

实体迁移技术有利于改善大规模分布式仿真系统的性能.首先分析了新一代仿真体系结构HLARTI对实体迁移支持上的不足和现有HLA兼容型仿真系统中实体迁移实现方法的缺陷;然后提出了在HLARTI下进行实体迁移的协议,设计了实体最新状态传输机制、迁移启动机制、迁移期间的消息转发、处理机制等;给出了协议的执行过程和支持实体迁移的仿真应用程序的实现框架;最后测试了协议的正确性.与相关研究相比,迁移协议解决了迁移过程中时间同步问题,不会丢失消息,且能保证TSO消息按时戳序处理.

关键词：实体迁移协议时间同步分布式仿真高层体系结构