检索结果-南通市图书馆

There is the second virus that causes tobacco leaf curl disease (not TbLCV-CHI) in the field

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第12期45卷 1131-1137页

作者： GONG Qianhong LIU Yule hong yiguo WANG Huanyu CHEN Gang CAI Jianhe Microbiology Institute Chinese Academy of SciencesBeijing 100080China Institute of Plant Protection Agricultural Academy of the Guangxi Zhuang Autonomous RegionNanning 530007China

Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in the Guangxi Zhuang Autonomous Region, China. This virus DNA-A contains 2 734 nt. Large intergenic region (LIR) contains 269 nt, the virus sense strand contains 2 open reading frames (ORFs): AV1 (115 aa) and AV2 (coat protein gene, CP, 256 aa), and the complementary sense strand contains 4 ORFs: AC1 (replicase gene, 361 aa), AC2 (transactivator, 134 aa), ACS (134 aa) and AC4 (97 aa). The virus belongs to one kind of subgroup III gemini- viruses from old world, and could be the Chinese tomato yellow leaf curl virus (TYLCV-CHI).

关键词： tobacco leaf curl disease geminivirus DNA sequence Chinese tomato yellow leaf curl virus (TYLCV-CHI) Chinese tobacco leaf curl virus (TbLCV-CHI).

The miR157-SPL-CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第5期64卷 1059-1075页

作者： Huihui Zhu Jiayi Wang Dan Jiang yiguo hong Jiming Xu Shaojian Zheng Jianli Yang Weiwei Chen State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China

Iron(Fe)homeostasis is critical for plant growth,development,and stress *** levels are tightly controlled by intricate regulatory networks in which transcription factors(TFs)play a central role.A series of basic helix-loop-helix(b HLH)TFs have been shown to contribute to Fe homeostasis,but the regulatory layers beyond b HLH TFs remain largely ***,we demonstrate that the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE(SPL)TF Sl SPL-CNR negatively regulates Fe-deficiency responses in tomato(Solanum lycopersicum)*** deficiency rapidly repressed the expression of Sl SPL-CNR,and Fe deficiency responses were intensified in two clustered regularly interspaced palindromic repeats(CRISPR)/CRISPRassociated protein 9-generated Sl SPL-CNR knockout lines compared to the *** transcriptome analysis identified 47 Fe deficiencyresponsive genes the expression of which is negatively regulated by Sl SPL-CNR,one of which,Slb HLH101,helps regulate Fe uptake *** SPLCNR localizes the nucleus and interacts with the GTAC and BOX 4(ATTAAT)motifs in the Slb HLH101 promoter to repress its *** of Sl SPL-CNR expression in response to Fe deficiency was well correlated with the expression of the micro RNA Slymi *** R157-overexpressing tomato lines displayed enhanced Fe deficiency responses,as did Sl SPL-CNR loss-of-function *** propose that the Slymi R157-Sl SPL-CNR module represents a novel pathway that acts upstream of Slb HLH101 to regulate Fe homeostasis in tomato roots.

关键词： Fe deficiency miR157 SPL-CNR tomato transcriptional repressor

CG hypermethylation of the bHLH39 promoter regulates its expression and Fe deficiency responses in tomato roots

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2023年第7期10卷 125-137页

作者： Huihui Zhu Guanghao Han Jiayi Wang Jiming Xu yiguo hong Li Huang Shaojian Zheng Jianli Yang Weiwei Chen State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China Laboratory of Cell&Molecular Biology Institute of Vegetable ScienceZhejiang UniversityHangzhou 310058China

Iron(Fe)is an essential micronutrient for all organisms,including plants,whose limited bioavailability restricts plant growth,yield,and nutritional *** the transcriptional regulation of plant responses to Fe deficiency have been extensively studied,the contribution of epigenetic modulations,such as DNA methylation,remains poorly ***,we report that treatment with a DNA methylase inhibitor repressed Fe deficiency-induced responses in tomato(Solanum lycopersicum)roots,suggesting the importance of DNA methylation in regulating Fe deficiency *** changes in the DNA methylome in tomato roots responding to short-term(12 hours)and long-term(72 hours)Fe deficiency identified many differentially methylated regions(DMRs)and DMR-associated *** DMRs occurred at CHH sites under short-term Fe deficiency,whereas they were predominant at CG sites following long-term Fe ***,no correlation was detected between the changes in DNA methylation levels and the changes in transcript levels of the affected genes under either short-term or long-term ***,one exception was CG hypermethylation at the bHLH39 promoter,which was positively correlated with its transcriptional *** agreement,we detected lower CG methylation at the bHLH39 promoter and lower bHLH39 expression in MET1-RNA interference lines compared with wild-type ***-induced gene silencing of bHLH39 and luciferase reporter assays revealed that bHLH39 is positively involved in the modulation of Fe ***,we propose that dynamic epigenetic DNA methylation in the CG context at the bHLH39 promoter is involved in its transcriptional regulation,thus contributing to the Fe deficiency response of tomato.

关键词： roots homeostasis treatment

Transcriptional and post‑transcriptional regulation of RNAi‑related gene expression during plant‑virus interactions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stress Biology 2022年第1期2卷 287-298页

作者： Qian Gong Yunjing Wang Zhenhui Jin yiguo hong Yule Liu MOE Key Laboratory of Bioinformatics and Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life Sciences Beijing 100084China Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China School of Science and the Environment University of WorcesterWorcester WR26AJUK School of Life Sciences University of WarwickCoventry CV47ALUK

As sessile organisms,plants encounter diverse invasions from pathogens including *** survive and thrive,plants have evolved multilayered defense mechanisms to combat virus ***,also known as RNA silencing,is an across-kingdom innate immunity and gene regulatory *** framework and crucial roles of RNAi in antiviral defense have been ***,it is largely unknown that how RNAi is transcriptionally regulated to initiate,maintain and enhance cellular silencing under normal or stress ***,insights into the transcriptional and post-transcriptional regulation of RNAi-related genes in different physiological processes have been *** this review,we integrate these new findings to provide updated views on how plants modulate RNAi machinery at the(post-)transcriptional level to respond to virus infection.

关键词： Transcriptional regulation RNAi Gene expression Virus Plant immunity

Virus-Induced Flowering In Dicot And Monocot Crops

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virus-Induced Flowering In Dicot And Monocot Crops

Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis

第三届全国植物开花·衰老与采后生物学大会

作者： yiguo hong Research Centre for Plant RNA Signaling College of Life and Environmental S ciencesHangzhou Normal University

Virus-induced flowering（VIF） assays exploit RNA or DNA viruses to express flowering time genes such as Flowering Locus T（FT） to induce flowering in plants1,2. Such plant virus-based techniques have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicot crops and woody fruit-trees. However, the insight into VIF, its potential as a powerful tool for dissecting florigenic proteins as well as its establishment in monocot crops remained to be elucidated. During my presentation, I will briefly explain different forms of virus-based technology for plant functional genomics, then move onto describing the mechanism and further applications of VIF in the short-day Nicotiana tabacum cultivar Maryland Mammoth, a dicot plant3. I will also present our latest work onextending this technology for the first time to proso millet, a monocot grass and wheat, a cereal crop4. These will lead to conclude that VIF represents a simple and efficient system to identify individual amino acids that are essential for FT-mediated floral induction and to test the ability of mono-and dicotyledonous FT genes and FT fusion proteins to induce flowering. VIF is also applicable to many economically important monocotyledonous food and biofuel crops.

关键词： Virus-Induced Dicot Monocot Crops

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2018年第5期60卷 376-381页

作者： Zhiming Yu Qiyuan Chen Weiwei Chen Xian Zhang Fengling Mei Pengcheng Zhang Mei Zhao Xiaohong Wang Nongnong Shi Stephen Jackson yiguo hong Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 310036China Warwick-Hangzhou Joint RNA Signaling Laboratory School of Life SciencesUniversity of WarwickCoventry CV4 7ALUK Worcester-Hangzhou Joint Molecular Plant Health Laboratory Institute of Science and the EnvironmentUniversity of WorcesterW R2 6AJUK

We report that a solo single-guide RNA （sgRNA） seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）/CRISPR -associated 9 （CRISRP/Casg） to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPLlo triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less-homologous sequences for multiple gene-editing in plants and other organisms.

关键词： RPL RNA

Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第2期61卷 244-252页

作者： Weiwei Chen Zhiming Yu Junhua Kong Hui Wang Yichen Li Mei Zhao Xiaohong Wang Qianqian Zheng Nongnong Shi Pengcheng Zhang Silin Zhong Paul Hunter Mahmut Tor yiguo hong Research Centre for Plant RNA Signaling College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310036 China State Key Laboratory of Agrobiotechnology School of Life Sciences The Chinese University of Hong Kong Hong Kong China Warwick-Hangzhou Joint RNA Signaling Laboratory School of Life Sciences University of Warwick Warwick CV4 7AL UK Worcester-Hangzhou Joint Molecular Plant Health Laboratory Institute of Science and the Environment University of Worcester WR2 6A J UK

Whole-genome bisulfite sequencing(WGBS)allows single-base resolution and genome-wide profiling of DNA methylation in plants and *** technology provides a powerful tool to identify genes that are potentially controlled by dynamic changes of DNA methylation and ***,naturally occurring epimutants are rare and genes under epigenetic regulation as well as their biological relevances are often difficult to *** tomato,fruit development and ripening are a complex process that involves epigenetic *** have taken the advantage of the tomato epimutant Colourless non-ripening(Cnr)and performed comparative mining of the WGBS datasets for the Cnr and Sl CMT3-silenced Cnr *** compared DNA methylation profiles for the promoter sequences of approximately 5,000 bp immediately upstream of the coding region of a list of20 *** methylated regions were found for some of these ***-induced gene silencing(VIGS)of differentially methylated gene Sl DET1 or Sl PDS resulted in unusual brown pigmentation in Cnr *** results suggest that comparative WGBS coupled with VIGS can be used to identify genes that may contribute to the colourless unripe phenotype of fruit in the Cnr epimutant.

关键词： tomato Cnr SlCMT3 SlDET1 SlPDS DNA methylation WGBS VIGS

在线全文

学校读者我要写书评

暂无评论

海洋氮循环中细菌的厌氧氨氧化

微生物学报 2009年第3期49卷 281-286页

作者：洪义国李猛顾继东中国科学院南海海洋研究所中国科学院热带海洋环境动力学重点实验室广东广州510301 香港大学生物科学学院中国香港

细菌厌氧氨氧化过程是在一类特殊细菌的厌氧氨氧化体内完成的以氨作为电子供体硝酸盐作为电子受体的一种新型脱氮反应。厌氧氨氧化菌的发现,改变人们对传统氮的生物地球化学循环的认识:反硝化细菌并不是大气中氮气产生的唯一生物类群。... 详细信息

细菌厌氧氨氧化过程是在一类特殊细菌的厌氧氨氧化体内完成的以氨作为电子供体硝酸盐作为电子受体的一种新型脱氮反应。厌氧氨氧化菌的发现,改变人们对传统氮的生物地球化学循环的认识:反硝化细菌并不是大气中氮气产生的唯一生物类群。而且越来越多的证据表明,细菌厌氧氨氧化与全球的氮物质循环密切相关,估计海洋细菌的厌氧氨氧化过程占到全球海洋氮气产生的一半左右。由于氮与碳的循环密切相关,因此可以推测,细菌的厌氧氨氧化会影响大气中的二氧化碳浓度,从而对全球气候变化产生重要影响。另外,由于细菌厌氧氨氧化菌实现了氨氮的短程转化,缩短了氮素的转化过程,因此为开发更节约能源、更符合可持续发展要求的废水脱氮新技术提供了生物学基础。

关键词：厌氧氨氧化(Anammox)细菌海洋氮循环厌氧氨氧化体

在线全文

学校读者我要写书评

暂无评论

深海冷泉古菌厌氧甲烷氧化研究进展

热带海洋学报 2021年第3期40卷 76-82页

作者：洪义国吴佳鹏广州大学大湾区环境研究院珠三角水质安全与保护教育部重点实验室广东广州510007

在富含甲烷水合物的海相冷泉沉积物中,古菌厌氧甲烷氧化作用(anaerobic oxidation of methane, AOM)越来越受到人们的重视。目前普遍认为, AOM是由嗜甲烷古菌和硫酸盐还原菌共同调节的生物地球化学过程。16S rRNA基因分析表明,包括AEME-... 详细信息

在富含甲烷水合物的海相冷泉沉积物中,古菌厌氧甲烷氧化作用(anaerobic oxidation of methane, AOM)越来越受到人们的重视。目前普遍认为, AOM是由嗜甲烷古菌和硫酸盐还原菌共同调节的生物地球化学过程。16S rRNA基因分析表明,包括AEME-1、AEME-2和AEME-3在内的多种甲烷古菌参与了AOM的过程,它们广泛分布于全球大洋海底缺氧带。AOM过程与全球环境变化密切相关,从深海底部冷泉区向上渗漏的甲烷气体,绝大部分在穿透缺氧带沉积层过程中被甲烷氧化古菌所消耗,有效减少了具有强烈温室效应的甲烷气体向大气的释放。对AOM生物地球化学过程的研究,在认识冷泉系统碳酸盐的形成机理、控制强温室气体甲烷从海底的渗漏和开发可燃冰新能源等方面具有重要意义。

关键词：厌氧甲烷氧化古菌海底冷泉甲烷渗漏