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Isolation and Characterization of Transcriptionally Active Ty1-copia Retrotransposons in Fragaria × ananassa

Agricultural Sciences in China 2010年第3期9卷 337-345页

作者： MA yue he Ping SUN Hai-yue ZHAO Gui-ling DAI hong-yan ZHANG Zhi-hong College of Horticulture Shenyang Agricultural University Shenyang 110866 P.R.China College of Forestry Shenyang Agricultural University Shenyang 110866 P.R.China

One possible mechanism suggested for somaclonal variation is the activation of transposable elements. The activation of retrotransposons by stresses and external changes is commonly observed in plants. In previous study, we isolated the reverse transcriptase （RT） gene sequences of Ty 1-copia retrotransposons from tissue culture strawberry （Fragaria x ananassa） plant, but not the transcriptionally active sequence. For further understanding the relationship between retrotransposon and somaclonal varation, in this study, we isolated the transcriptionally active RT gene sequences from strawberry plants subjected to different abiotic stresses. These retrotransposons were activated by spraying strawberry leaves with 2 mmol L^-1 salicylic acid （SA）, 50 mmol L^-1 methyl jasmonate （MeJA）, 50 mmol L^-1 abscisic acid （ABA）, 50 mmol L^-1 2,4- dichlorophenoxyacetic acid （2,4-D） or by inducing callus growth in 2 types of MS media： first medium supplemented with 0.5 mg L^-1 6-benzylaminopurine （6-BA）, 0.5 mg L^-1 gibberellic acid （GA3）, 1.0 mg L^-1 thidiazuron （TDZ）, and 0.1 mg L^-1 2,4-D, and the second medium supplemented with 0.5 mg L^-1 6-BA, 0.5 mg L^-1 GA3, 2.0 mg L^-1 TDZ, and 0.02 mg L^-1 indole butyric acid （1BA）. Analysis of gene sequences of 17 RTs revealed that none of them contained stop codons and/or indels disrupting the reading frame. These different stress-origin transcriptionally active RTs were remarkably similar to each other- FATEXP2-8 and FATEYS9-7 showed 100% sequence identity. Analysis of pylogenetic of these transcriptionally active RTs and the RT sequences from genome showed that there were close phylogenetic relationships of most of the transcriptionally active RTs. The results of this study have contributed to the background information necessary for future studies for evaluating the relationship between retrotransposons and somaclonal variation.

关键词： transcription activated callus culture Ty1-copia retrotransposons strawberry stress

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Spatiotemporal characteristics and influencing factors of vegetation water use efficiency on the Tibetan Plateau in 2001–2020

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Journal of Geographical Sciences 2025年第1期35卷 39-64页

作者： he Chenyang WANG Yanjiao YAN Feng LU Qi School of Ecological and Environmental Science East China Normal University Institute of Ecological Conservation and Restoration Chinese Academy of Forestry National Climate Center China Meteorological Administration

Water use efficiency （WUE）,as a pivotal indicator of the coupling degree within the carbon–water cycle of ecosystems,holds considerable importance in assessment of the carbon–water balance within terrestrial ***,in the context of global warming,WUE evolution and its primary drivers on the Tibetan Plateau remain *** study employed the ensemble empirical mode decomposition method and the random forest algorithm to decipher the nonlinear trends and drivers of WUE on the Tibetan Plateau in 2001–*** indicated an annual mean WUE of 0.8088 gC/mm·m2across the plateau,with a spatial gradient reflecting decrease from the southeast toward the *** manifesting monotonous trends of increase or decrease in WUE accounted for 23.64%and 9.69%of the total,***,66.67%of the region exhibited trend reversals,i.e.,39.94%of the area of the Tibetan Plateau showed transition from a trend of increase to a trend of decrease,and 26.73%of the area demonstrated a shift from a trend of decrease to a trend of *** factors accounted for 70.79%of the variability in *** leaf area index and temperature served as the major driving forces of WUE variation.

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Multiple Tin Compounds Modified Carbon Fibers to Construct heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang Guo Di Lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun he Hua Guo Guanglei Wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

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Epigenetics in the modern era of crop improvements

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Science China Life Sciences 2025年

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng Yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng he Hang he Shengbo he Xinjian he Yan he yuehui he Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zhengyi Xu Wenhao Yan hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Daoxiu Zhou Ming Zhou yue Zhou Bo Zhu Jiankang Zhu Qikun Liu College of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences Key Laboratory of Seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced Agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University Key Laboratory of Seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life Sciences Henan University State Key Laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design Breeding China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Sanya Institute of China Agricultural University Hainan Yazhou Bay Seed Laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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Characteristic changes in astrocyte properties during astrocyte-to-neuron conversion induced by NeuroD1/Ascl1/Dlx2

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Neural Regeneration Research 2025年第6期20卷 1801-1815页

作者： Qing he Zhen Wang Yuchen Wang Mengjie Zhu Zhile Liang Kanghong Zhang Yuge Xu Gong Chen GHM Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina

Direct in vivo conversion of astrocytes into functional new neurons induced by neural transcription factors has been recognized as a potential new therapeutic intervention for neural injury and degenerative disorders. However, a few recent studies have claimed that neural transcription factors cannot convert astrocytes into neurons, attributing the converted neurons to pre-existing neurons mis-expressing transgenes. In this study, we overexpressed three distinct neural transcription factors––NeuroD1, Ascl1, and Dlx2––in reactive astrocytes in mouse cortices subjected to stab injury, resulting in a series of significant changes in astrocyte properties. Initially, the three neural transcription factors were exclusively expressed in the nuclei of astrocytes. Over time, however, these astrocytes gradually adopted neuronal morphology, and the neural transcription factors was gradually observed in the nuclei of neuron-like cells instead of astrocytes. Furthermore,we noted that transcription factor-infected astrocytes showed a progressive decrease in the expression of astrocytic markers AQP4(astrocyte endfeet signal), CX43(gap junction signal), and S100β. Importantly, none of these changes could be attributed to transgene leakage into preexisting neurons. Therefore, our findings suggest that neural transcription factors such as NeuroD1, Ascl1, and Dlx2 can effectively convert reactive astrocytes into neurons in the adult mammalian brain.

关键词： aquaporin-4 Ascl1 astrocyte cortex Dlx2 gap junction glia-to-neuron conversion neural regeneration NeuroD1 reprogramming

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Enhanced autophagic clearance of amyloid-βvia histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer's disease in vitro and in vivo

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Neural Regeneration Research 2025年第9期20卷 2633-2644页

作者： Zhimin Long Chuanhua Ge yueyang Zhao Yuanjie Liu Qinghua Zeng Qing Tang Zhifang Dong Guiqiong he Institute of Neuroscience School of Basic Medical SciencesChongqing Medical UniversityChongqingChina Department of Anatomy Chongqing Medical UniversityChongqingChina Department of Physiology Chongqing Medical UniversityChongqingChina Pediatric Research Institute Children’s Hospital of Chongqing Medical UniversityChongqingChina Ministry of Education Key Laboratory of Child Development and Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina National Clinical Research Center for Child health and Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina

Recent studies have suggested that abnormal acidification of lysosomes induces autophagic accumulation of amyloid-βin neurons,which is a key step in senile plaque ***,resto ring normal lysosomal function and rebalancing lysosomal acidification in neurons in the brain may be a new treatment strategy for Alzheimer's *** acetylation/deacetylation plays a central role in lysosomal ***,we show that inhibiting the classic microtubule deacetylase histone deacetylase 6 with an histone deacetylase 6 shRNA or thehistone deacetylase 6 inhibitor valproic acid promoted lysosomal reacidification by modulating V-ATPase assembly in Alzheimer's *** rthermore,we found that treatment with valproic acid markedly enhanced *** clearance of amyloid-βaggregates,and ameliorated cognitive deficits in a mouse model of Alzheimer's *** findings demonstrate a previously unknown neuroprotective mechanism in Alzheimer's disease,in which histone deacetylase 6 inhibition by valproic acid increases V-ATPase assembly and lysosomal acidification.

关键词： Alzheimer's disease amyloid-β APP/PS1 mice autophagy cognitive impairment histone deacetylase 6 lysosomal acidification microtubule acetylation valproic acid V-ATPase

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基于列生成算法的无人机任务分配

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指挥与控制学报 2019年第2期5卷 147-152页

作者：李瑞阳王智学何红悦邓巧雨陆军工程大学指挥控制工程学院

任务分配是无人机完成多样化军事任务的重要保证.为了合理制定任务方案,有效降低飞行成本,根据无人机在军事行动中需要运输的物资数量及目的地位置,建立了追求飞行成本最小并考虑时间约束的无人机任务分配优化模型,应用列生成算法对飞... 详细信息

任务分配是无人机完成多样化军事任务的重要保证.为了合理制定任务方案,有效降低飞行成本,根据无人机在军事行动中需要运输的物资数量及目的地位置,建立了追求飞行成本最小并考虑时间约束的无人机任务分配优化模型,应用列生成算法对飞行方案进行优化选择.设计实验对列生成算法和差分进化算法进行对比分析,结果表明,列生成算法具有更高的求解精度.在大规模任务分配问题中,利用该算法对飞行方案进行优化选择,显著提高军事任务完成效率.

关键词：无人机任务分配路径优化列生成算法

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Mechanism of inflammatory response and therapeutic effects of stem cells in ischemic stroke:current evidence and future perspectives

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Neural Regeneration Research 2025年第1期20卷 67-81页

作者： Yubo Wang Tingli Yuan Tianjie Lyu Ling Zhang Meng Wang Zhiying he Yongjun Wang Zixiao Li Vascular Neurology Department of NeurologyBeijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Shanghai Engineering Research Center of Stem Cells Translational Medicine ShanghaiChina National Center for healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Institute for Regenerative Medicine Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital healthcare for Neurological Diseases BeijingChina

Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger ***,in particular,bears a high burden of stroke *** recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its *** review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and ***,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell ***,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient *** cell therapy may potentially become an important component of ischemic stroke treatment.

关键词： cell therapy immune cell inflammatory ischemic stroke stem cell

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高HCY血症对ACS患者LPA、炎症因子、血小板参数及DAPT治疗的影响

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热带医学杂志 2020年第9期20卷 1206-1209,1235页

作者：李瑞瑞刘恩香李晶晶何洪月沧州市人民医院心内一科河北沧州061000

目的探究高同型半胱氨酸(HCY)血症对急性冠脉综合征(ACS)患者溶血磷脂酸(LPA)水平及对双联抗血小板药物治疗(DAPT)的影响。方法选取沧州市人民医院2012年8月-2017年8月收治的110例ACS患者,根据HCY水平(是否≥15μmol/L)分为高HCY组(n=47... 详细信息

目的探究高同型半胱氨酸(HCY)血症对急性冠脉综合征(ACS)患者溶血磷脂酸(LPA)水平及对双联抗血小板药物治疗(DAPT)的影响。方法选取沧州市人民医院2012年8月-2017年8月收治的110例ACS患者,根据HCY水平(是否≥15μmol/L)分为高HCY组(n=47)和非高HCY组(n=63),所有患者均行DAPT干预,对比两组患者治疗前后临床各指标差异情况,分析HCY与临床其他指标的相关性,并比较两组治疗后12~24个月缺血事件和出血事件的发生率。结果治疗前,两组患者年龄、性别、体质量指数、ACS家族史、是否合并高血压、PRECISEDAPT评分、左心室射血分数(LVEF)、血脂指标[总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]比较差异无统计学意义(P>0.05),两组HCY、LPA、炎性因子[白介素-6(IL-6)、超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)]、血管内皮生长因子(VEGF)、血小板参数[血小板平均体积(MPV)、血小板分布宽度(PDW)、血小板计数(PLT)、血小板聚集率(PAR)]比较差异具有统计学意义(P<0.05)。治疗后,两组PRECISE-DAPT评分、HCY、LPA、LVEF、TC、TG、HDL-C、LDL-C、IL-6、hs-CRP、TNF-α、VEGF、MPV、PDW、PLT及PAR比较差异均有统计学意义(P<0.05)。相关性分析结果显示,HCY与PRECISE-DAPT评分、LPA、TC、TG、LDL-C、IL-6、hs-CRP、TNF-α、MPV、PDW、PAR存在显著正相关性(P<0.05),与HDL-C、VEGF、PLT存在显著负相关性(P<0.05)。高HCY组的缺血事件(心肌梗死、心源性死亡、卒中)(17.0%vs. 4.8%)和出血事件(10.6%vs. 1.6%)发生率明显高于对照组,差异有统计学意义(x2=4.495、4.276,P均<0.05)。结论高HCY血症导致ACS患者机体LPA、炎症水平升高,血小板聚集和血管内皮功能损伤等,从而降低DAPT的治疗效果,增加患者预后不良事件的发生。

关键词：急性冠脉综合征高同型半胱氨酸血症溶血磷脂酸双联抗血小板药物治疗

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Nano high-entropy oxide cathode with enhanced stability for direct borohydride fuel cells

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Journal of Energy Chemistry 2025年第1期100卷 309-316页

作者： Lei Zhang Lingfeng Kuang Lianke Zhang Wen Chu Haiying Qin Jing Zhang Junjing he Hualiang Ni hongzhong Chi New Energy Materials Research Center College of Materials and Environmental Engineering Hangzhou Dianzi University School of Materials Science and Engineering Zhejiang University Key Laboratory of MEMS of Ministry of Education School of Integrated Circuits Southeast University

High-entropy materials have become high-activity electrocatalysis owing to their high-entropy effect and multiple active ***,we synthesize a series of carbon-supported nano high-entropy oxides（heOs/C）,specifically （PtFeCoNiCu）O/C,using a carbothermal shock （CTS） method for application as a cathode catalyst in direct borohydride fuel cells （DBFCs）.The microstructure of the prepared catalysts was characterized by X-ray photoelectron spectroscopy,X-ray absorption fine structure,and transmission electron *** prepared （PtFeCoNiCu）O/C,with particle sizes ranging from 2 to 4 nm,demonstrates 3.94 transferred electrons towards the oxygen reduction reaction in an alkaline environment,resulting in a minimal H2O2yield of 2.6%.Additionally,it exhibits a Tafel slope of 61 mV dec-1,surpassing that of commercial Pt/C (82 mV dec-1).Furthermore,after 40,000 cycles of cyclic voltammetry（CV） testing,the half-wave potential of （PtFeCoNiCu）O/C shows a positive shift of 3 mV,with no notable decline in the limiting current *** （PtFeCoNiCu）O/C is used as a cathode catalyst in DBFCs,the DBFC achieves a maximum power density of 441 mW cm-2at 60°C and sustains a cell voltage of approximately 0.73 V after 52 h at 30°*** findings confirm that heO/C is a promising cathode catalyst for DBFCs.

关键词： Direct borohydride fuel cells (DBFC)

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