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Postbiotics as potential new therapeutic agents for sepsis

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Burns & Trauma 2023年第1期11卷 510-523页

作者： Xiran Lou Jinfang Xue Ruifei Shao Chunyan Mo Fuping Wang guobing chen Medical School Kunming University of Science and Technology727 Jingming South RoadChenggong DistrictKunming 650500China Department of Emergency Medicine The First People’s Hospital of Yunnan Province157 Jinbi RoadXishan DistrictKunming 650034China

Sepsis is the main cause of death in critically ill patients and gut microbiota dysbiosis plays a crucial role in *** the one hand,sepsis leads to the destruction of gut microbiota and induces and aggravates terminal organ *** the other hand,the activation of pathogenic gut flora and the reduction in beneficial microbial products increase the susceptibility of the host to *** probiotics or fecal microbiota transplantation preserve gut barrier function on multiple levels,their efficacy in sepsis with intestinal microbiota disruptions remains *** consist of inactivated microbial cells or cell *** possess antimicrobial,immunomodulatory,antioxidant and antiproliferative ***-targeted therapy strategies,such as postbiotics,may reduce the incidence of sepsis and improve the prognosis of patients with sepsis by regulating gut microbial metabolites,improving intestinal barrier integrity and changing the composition of the gut *** offer a variety of mechanisms and might even be superior to more conventional‘biotics’such as probiotics and *** this review,we present an overview of the concept of postbiotics and summarize what is currently known about postbiotics and their prospective utility in sepsis ***,postbiotics show promise as a viable adjunctive therapy option for sepsis.

关键词： Sepsis Gut microbiota Dysbiosis Postbiotics

Dl-3-n butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance

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Stroke & Vascular Neurology 2022年第3期7卷 222-236页

作者： Kangping Song Xiuli Zeng Xiaomei Xie Rongxuan Zhu Jianye Liang guobing chen Li’an Huang Department of Neurology Jinan University First Affiliated HospitalGuangzhouChina Medical Imaging Center Jinan University First Affiliated HospitalGuangzhouChina Institute of Geriatric Immunology Medical college of Jinan UniversityGuangzhouChina

Background Cerebral venous disorder may have a harmful effect on ischaemic stroke;however,the underlying mechanism remains to be *** Dl-3-n butylphthalide is a multitarget agent for antiischaemic stroke,its neuroprotective role in brain ischaemia accompanied by brain venous disturbance remains *** this study,we induced cerebral venous disturbance by the occlusion of bilateral external jugular veins(EJVs)to explore the potential mechanism of the adverse effects of cerebrovenous disorders in cerebral infarction and explore the protective effect of Dl-3-n butylphthalide on cerebral infarction accompanied through cerebral venous *** Cerebral venous disturbance was induced in Sprague-Dawley rats through the permanent occlusion of bilateral EJVs,and cerebral ischaemic stroke was induced through the permanent occlusion of the right cortical branches of the middle cerebral artery.2,3,5-triphenyltetrazolium chloride staining,MRI,Evans blue extravasation and behavioural test were performed to evaluate infarction volume,cerebral blood flow(CBF),blood-brain barrier(BBB)integrity and neurological *** staining and western blot analysis were performed to detect loss of neuron,endothelial cells,pericytes and tight *** Bilateral EJVs occlusion did not cause cerebral infarction;however,it increased the infarction volume compared with the simple middle cerebral artery occlusion(MCAO)group,accompanied by severe neuron loss,worse neurological function,lower CBF,increased EJVs pressure,exacerbated Evans blue extravasation and brain oedema,as well as attenuated ***-3-n butylphthalide displayed a neuroprotective effect in rats with MCAO accompanied by EJVs occlusion by reducing neuron loss,accelerating CBF restoration,promoting angiogenesis and relieving BBB *** Bilateral EJVs occlusion did not significantly affect normal rats but aggravated brain damage in the case of ischaemic ***

关键词： protective infarction disturbance

Analyzing the phenotypic and functional complexity of lymphocytes using CyTOF （cytometry by time-of-flight）

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Cellular & Molecular Immunology 2012年第4期9卷 322-323页

作者： guobing chen Nan-ping Weng Laboratory of Molecular Biology and Immunology National Institute on Aging National Institutes of Health Baltimore MD USA

The human immune system is equipped with vast numbers of lymphocytes possessing distinct antigen specificities. The enormous repertoire and heterogeneity of lymphocytes ensures their ability to effec- tively fight against external microbial inva- ders, as well as internal aberrant cells. However, the understanding of the specific phenoWpes and functions of lymphocytes is hindered by the lack of tools that can be used to visualize this complexity.

关键词：钙调蛋白结合蛋白质类理解力磷蛋白磷酸酶类淋巴细胞免疫系统人口特征血气分析胰岛素人类

Analysis of the clinical features and risk factors of death in patients with mushroom poisoning

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Emergency and Critical Care Medicine 2023年第1期3卷 18-22页

作者： Yan Yang Ruifei Shao Jinfang Xue Xiran Lou Deyuan Ning guobing chen Medical Faculty Kunming University of Science and TechnologyKunmingYunnanChina Emergency Department The First People’s Hospital of Yunnan ProvinceKunmingYunnanChina

Background:Wild edible mushrooms are rich in nutrition and popular with people;however,few laboratory studies are available about the predictability of outcomes among patients with mushroom ***,this study aimed to analyze the clinical features and death risk factors of patients with mushroom ***:Patients with mushroom poisoning admitted to the hospital from 2015 to 2021 were retrospectively ***:A total of 197 patients with mushroom poisoning were enrolled in this study,of which 100(50.76%)were males,and the mortality was 10.66%(21/197).Patients who died were more likely to have demonstrated a long latency,high alanine aminotransferase,aspartate aminotransferase,direct bilirubin,total bilirubin(TB),activated partial thromboplastin time,prothrombin time,international normalized ratio,creatinine,and blood urea *** logistic regression analysis indicated that TB level greater than or equal to 34.2μmol/L had the greatest lethal risk and could increase the risk of death by 14.588 times(odds ratio:15.588;95%confidence interval:2.088-116.351),which indicated that TB was an independent risk factor of death in patients with acute mushroom ***:Bilirubin concentration was associated with the increased likelihood of *** bilirubin was the independent risk factor of mushroom poisoning.

关键词： Clinical features Mushroom poisoning Prognosis Risk factors

Expression,Purification and Identification of Recombinant Mouse Interleukin 21 Protein in ***

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Cellular & Molecular Immunology 2006年第4期3卷 311-315页

作者： Weiguo Tang guobing chen Qing Gu Jing Pan Wutong Wu School of Life Science and Technology China Pharmaceutical University Nanjing 210009 China Jinling Pharmaceutical Co. Ltd. NanJing 210009 China

Interleukin 21 （IL-21） is a novel type I cytokine that is significantly homologous to IL-2, IL-4 and IL-15. Its receptor complex contains γc chain which is also a component of receptors for IL-2, IL-4, IL-7, IL-9 and IL-15, so there may be overlapping or relevancies in their biological functions. IL-21 is capable of co-stimulating mature T cells, B cells, NK cells, and of stimulating CD16 expression on the surface of NK cells to induce ADCC in innate immune response. It can also strengthen the anti-tumor effect of the cellular immunity, especially v/a enhancing the activities of NK and antigen specific CTL cells. Thus, IL-21 is a potential useful therapeutic molecule for immunotherapy of malignancies, by eliciting innate and adaptive anti-tumor immune responses in tumor-bearing hosts. In order to study the biological functions of IL-21, we constructed a mIL-21 prokaryotic expression plasmid and expressed the recombinant mIL-21 protein in E. coli in present study. The recombinant plasmid pET28a/mIL-21 with a carboxyl terminal His-tag was subcloned from the pcDNA3.1/mIL-21 and expressed in E. coli. The induced protein was detected by SDS-PAGE, and identified by Western-blot assay with anti-mIL-21 antibody. The recombinant protein was purified v/a Ni^＋ affinity chromatography, and renatured with GSH/GSSG system. Our mouse T cell proliferation experiment showed that the recombinant mIL-21 protein could enhance the mouse T cell proliferation either by itself alone or in the presence of Con A.

关键词： interleukin 21 gene expression protein purification

The role of Ulk1 in T cell development,differentiaton and function

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The role of Ulk1 in T cell development,differentiaton and fu...

SARS-CoV-2 variant B.1.1.7 caused HLA-A2+CD8+T cell epitope mutations for impaired cellular immune response

第十四届全国免疫学学术大会

作者： Yudai Xu guobing chen Deparment of Microbiology and Immunology School of MedicineJinan University Jinan University

ULK1 is a serine/threonine protein kinase that mediates autophagy,which plays an important role in immune ***,it still remains unclear whether ULK1-induced autophagy is involved in the regulation of T *** this study we used UIk1 mice to investigate the role of ULK1 in T cells development and *** found that UIk1 mice displayed reduced proportion of peripheral T and B cells but not in Neutrophils and natural killer *** young Ulk1 mice exhibited increased DN3 but decreased DN4 thymocytes,which indicated the severe block in early thymic ***1 deficiency also impaired mice peripheral T *** proportion of CD4and CD8T cells decreased in Ulk1 mice *** bone marrow chimeras experiments exhibited normal T cell proportion in spleen in the group of UIk1 adoptive transferred into WT *** findings reveal an essential role for ULK1 in T cell development,survival,and function.

关键词： autophagy ULK1 T cells development

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SARS-CoV-2 variant B.1.1.7 caused HLA-A2+CD8+T cell epitope ...

用于复杂有机化合物热容预测的一个新的拓扑指数

第十四届全国免疫学学术大会

作者： chanchan xiao lipeng mao zhigang wang guobing chen Department of Microbiology and Immunology 暨南大学附属华侨医院

The rapid spreading of the newly emerged SARS-CoV-2 variant,B.1.1.7,highlighted the requirements to better understand adaptive immune responses to this *** CD8T cell responses play an important role in disease resolution and modulation in COVID-19 patients,it is essential to address whether these newly emerged mutations would result in altered immune *** we evaluated the immune properties of the HLA-A2 restricted CD8T cell epitopes containing mutations from B.1.1.7,and furthermore performed a comprehensive analysis of the SARS-CoV-2 specific CD8+T cell responses from COVID-19 convalescent patients and SARS-CoV-2 vaccinees recognizing the ancestral Wuhan strain compared to ***,most of the predicted CD8T cell epitopes showed proper binding with HLA-A2,while epitopes from B.1.1.7 had lower binding capability than those from the ancestral *** addition,these peptides could effectively induced the activation and cytotoxicity of CD8T *** results further showed that at least two site mutations in B.1.1.7 resulted in a decrease in CD8T cell activation and a possible immune evasion,namely Al 708 D mutation in ORF1 ab and I2230 T mutation in ORF1 *** current analysis provides information that contributes to the understanding of SARS-CoV-2-specific CD8T cell responses elicited by infection of mutated strains or vaccination.

关键词： SARS-CoV-2 CD8+T cell epitope B.1.1.7 variations Antigen presentation deficiency immune escape

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化工学报 1999年第2期50卷 280-286页

作者：任碧野陈国斌许友华南理工大学材料科学研究所广州510641 岳阳大学化工系岳阳414000

引言化合物的理化性质主要由分子的几何结构和电子结构所决定,如何直接从分子结构预测化合物的理化性质已成为近几年来计算机化学的主要研究内容之一.利用拓扑指数与化合物的理化性质建立定量结构-性质/活性关系(QSPR/QSAR)来对化合物... 详细信息

引言化合物的理化性质主要由分子的几何结构和电子结构所决定,如何直接从分子结构预测化合物的理化性质已成为近几年来计算机化学的主要研究内容之一.利用拓扑指数与化合物的理化性质建立定量结构-性质/活性关系(QSPR/QSAR)来对化合物的性质/活性进行预测和评估则是一条十分简便的途径,引起了人们的极大兴趣.目前,拓扑指数法已广泛用于化学、化工、生物化学、环境科学、药学以及毒理学等领域,拓扑指数(即图不变量)就是用某种数学量来表征化合物的分子结构.虽然目前已提出了一百多种拓扑指数,但真正能适应含杂原子、多重键等复杂体系的却廖廖无几.为此,作者最近提出了一个新的拓扑指数Xu,并成功地用于烷烃的沸点等理化性质的预测.本文进一步将Xu指数推广到含多重键、杂原子的化合物体系以期对化合物的热容进行预测.

关键词：图论拓扑指数有机化合物热容预测 QSPR

历史教学中的叙述——以新课程人民版历史教科书的使用为例

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历史教学 2007年第1期 17-19页

作者：陈国兵界首高级中学江苏高邮225611

在课堂教学活动中，学生知识信息的来源主要有两种，一是历史教材，二是教师的叙述。过去，许多教师把教学过程简单化。把自己的叙述与教材完全等同起来，我们称之为“照本宣科”。这样的教学方法使得历史课堂索然无味，严重扼杀了学生... 详细信息

在课堂教学活动中，学生知识信息的来源主要有两种，一是历史教材，二是教师的叙述。过去，许多教师把教学过程简单化。把自己的叙述与教材完全等同起来，我们称之为“照本宣科”。这样的教学方法使得历史课堂索然无味，严重扼杀了学生学习历史的兴趣。其实，课堂教学中，教师的叙述是最为灵动的部分，虽然受到教科书的影响，但与教科书有着严格的区别。由于个人创作的存在，又受知识控制能力和意趣的影响，不同教师的叙述所产生的教育效果迥异。目前，在新课程改革的背景下，教科书体系发生了明显的变化，专题体例和模块教学所带来的知识点分散、残缺、重复等问题给教师的课堂叙述带来了新的问题。因此，怎样优化课堂叙述，把历史知识转化为学生的智慧和情感便成为新课程改革中的重要话题。本文结合人民版教科书的使用实例，谈谈自己的一些课改体会。

关键词：历史教学教材课堂叙述