SARS-CoV-2 variant B.1.1.7 caused HLA-A2+CD8+T cell epitope mutations for impaired cellular immune response
作者单位：Department of Microbiology and Immunology 暨南大学附属华侨医院
会议名称：《第十四届全国免疫学学术大会》
会议日期：2021年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：SARS-CoV-2 CD8+T cell epitope B.1.1.7 variations Antigen presentation deficiency immune escape
摘 要：The rapid spreading of the newly emerged SARS-CoV-2 variant,B.1.1.7,highlighted the requirements to better understand adaptive immune responses to this *** CD8T cell responses play an important role in disease resolution and modulation in COVID-19 patients,it is essential to address whether these newly emerged mutations would result in altered immune *** we evaluated the immune properties of the HLA-A2 restricted CD8T cell epitopes containing mutations from B.1.1.7,and furthermore performed a comprehensive analysis of the SARS-CoV-2 specific CD8+T cell responses from COVID-19 convalescent patients and SARS-CoV-2 vaccinees recognizing the ancestral Wuhan strain compared to ***,most of the predicted CD8T cell epitopes showed proper binding with HLA-A2,while epitopes from B.1.1.7 had lower binding capability than those from the ancestral *** addition,these peptides could effectively induced the activation and cytotoxicity of CD8T *** results further showed that at least two site mutations in B.1.1.7 resulted in a decrease in CD8T cell activation and a possible immune evasion,namely Al 708 D mutation in ORF1 ab and I2230 T mutation in ORF1 *** current analysis provides information that contributes to the understanding of SARS-CoV-2-specific CD8T cell responses elicited by infection of mutated strains or vaccination.