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Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations

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Insect Science 2020年第6期27卷 1158-1172页

作者： huan Yu Zi-Qi Li Yi-Yi Ou-Yang guo-hua huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Apoptosis plays critical roles in multiple biological processes in multicellular *** are known as important participators and regulators of ***,four novel caspase genes of Spodoptera exigua were cloned and characterized which were designated as SeCasp-1,SeCasp-6,ScCasp-7 and *** of the putative encoded protein sequences of these SeCasps indicated that SeCasp-1 and ScCasp-7 were possible homologs of executor caspases;SeCasp-8 was a possible homolog of initiator caspases;and SeCasp-6 was a unique caspase of *** that shares low similarity with all the identified insect *** on baculovirus expression system analyses,SeCasp-1 exhibited similar caspase activity to human caspase-1,-3,-4,-6,-8 and-9;SeCasp-6 presented similar caspase activity to human caspase-2,-3,-4,-6,-8 and-9;SeCasp-7 exhibited similar caspase activity to human caspase-2,-3 and-6;and SeCasp-8 presented similar caspase activity only to human *** with diiTcrent chemicals revealed that SeCasp-1 showed extreme upregulation after 24 h in the treated fat body cell line(IOZCAS-Spex-II)of *** expression analysis revealed that SeCasp-1 was highly transcribed in the larval stages,while SeCasp-6,SeCasp-7,SeCasp-H were *** in vivo detection of the relative expression levels of SeCasps in *** larvae inoculated with different pathogens suggested that SeCasp-J was sensitive to Bacillus thuringiensis infection and that SeCasp-6 was sensitive to baculovirus ***-7 and SeCasp-H showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection.

关键词： apoptosis apoptotic inducing insect caspase SeCasp Spodoptera exigua

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Floating elbow combining ipsilateral distal multiple segmental forearm fractures: A case report

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World Journal of Clinical Cases 2021年第14期9卷 3372-3378页

作者： guo-hua huang Jiang-An Tang Tie-Yi Yang Yue Liu Department of Orthopedics Shanghai Pudong New Area Gongli HospitalAffiliated to the Second Military Medical UniversityShanghai 200135China

BACKGROUND Floating elbow along with ipsilateral multiple segmental forearm fracture is a rare and high-energy injury,although elbow dislocation or fracture of the ulna and radius may occur *** SUMMARY We report the case of a 37-year-old woman with open(IIIA)fracture of the right distal humerus with multiple shaft fractures of the ipsilateral radius and ulna with a history of falling from a height of almost 20 m from a *** providing advanced trauma life support,damage control surgery was performed to debride the arm wound and temporarily stabilize the right upper limb with external fixators in the emergency operating ***,one-stage internal fixation of multiple fractures was performed with normal values of biochemical indicators and reduction in limb *** patient achieved good outcome at the 7 mo *** One-or two-stage treatment must be performed according to the type of injury;we efficiently used the“damage control principle.”

关键词： Floating forearm Advanced trauma life support Internal fixation Multiple fractures Open fracture Case report

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Genome Analysis of Dasineura jujubifolia Toursvirus 2,A Novel Ascovirus

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Virologica Sinica 2020年第2期35卷 134-142页

作者： Jun Wang Minglu Yang Haibing Xiao guo-hua huang Fei Deng Zhihong Hu State Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Tarim University Alar 843300China Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Institute of VirologyHunan Agricultural UniversityChangsha 410128China

So far,ascoviruses have only been identified from Lepidoptera host insects and their transmission vectors-endoparasitic ***,we reported the first finding of a complete novel ascovirus genome from a Diptera insect,Dasineura ***,sequence fragments with homology to ascoviruses were incidentally identified during metagenomic sequencing of the mitochondria of ***(Cecidomyiidae,Diptera)which is a major pest on Ziziphus *** a full circular viral genome was assembled from the metagenomic data,which has an A+T percentage of 74%and contains 142,600 bp with 141 open reading frames(ORFs).Among the 141 ORFs,37 were conserved in all sequenced ascoviruses(core genes)including proteins predicted to participate in DNA replication,gene transcription,protein modification,virus assembly,lipid metabolism and ***-gene families including those encode for baculovirus repeated open reading frames(BROs),myristylated membrane proteins,RING/U-box E3 ubiquitin ligases,and ATP-binding cassette(ABC)transporters were found in the virus *** analysis showed that the newly identified virus belongs to genus Toursvirus of Ascoviridae,and is therefore named as Dasineura jujubifolia toursvirus 2(DjTV-2a).The virus becomes the second reported species of the genus after Diadromus pulchellus toursvirus 1(DpTV-1a).The genome arrangement of DjTV-2a is quite different from that of DpTV-1a,suggesting these two viruses separated in an early time of *** results suggest that the ascoviruses may infect a much broader range of hosts than our previous knowledge,and shed lights on the evolution of ascoviruses and particularly on that of the toursviruses.

关键词： Dasineura jujubifolia toursvirus 2(DjTV-2a) Ascovirus Toursvirus

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Melanization induced by Heliothis virescens ascovirus 3h promotes viral replication

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Insect Science 2021年第2期28卷 472-484页

作者： Zi-Qi Li Xiao-Hui Song Min Wang Shu Wang guo-hua huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Melanization is an important innate immune defense mechanism of insects,which can kill invading *** pathogens,for their survival and reproduction,inhibit the melanization of the ***,our results suggested that after infection with Heliothis virescens ascovirus 3h(HvAV-3h),the speed of melanization in infected Spodoptera exigiia larval hemolymph was accelerated and that the phenoloxidase(PO)activity of hemolymph in larvae infected with HvAV-3h increased significantly(1.20-fold at 96 hpi,1.52-fold at 120 hpi,1.23-fold at 144 hpi,1.12-fold at 168 hpi).The transcription level of the gene encoding *** prophenoloxidase-1(SePPO-1 gene)was upregulated dramatically in the fat body during the middle stage of *** addition,when melanization was inhibited or promoted,the replication of HvAV-3h was inhibited or promoted,*** conclusion,infection with HvAV-3h can markedly induce melanization in the middle stage of infection,and melanization is helpful for HvAV-3h viral replication.

关键词： Heliothis virescens ascovirus 3h melanization Spodoptera exigua viral replication

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In Vitro Infectious Risk Assessment of Heliothis virescens ascovirus 3j(HvAV-3j) toward Non-target Vertebrate Cells

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Virologica Sinica 2019年第4期34卷 423-433页

作者： huan Yu Yi-Yi Ou-Yang Ni Li Madoka Nakai guo-hua huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangsha 41012& China College of Plant Protection Hunan Agricultural UniversityChangsha 41012& China Tokyo University of Agriculture and Technology SaiwaiFuchuTokyo 183-8509Japan

As specific pathogens of noctuid pests,including Spodoptera exigua,***,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of *** this study,the infectivity of Heliothis virescens ascovirus 3j(HvAV-3j)on insect and mammalian cells was ***-3j infection induced drastic morphological changes in Sf9,HzAMl,SeFB,and HaFB cells,including swelling and detachmen Notably,the latter phenomena did not occur in HvAV-3j-inoculated mammalian cells(HEK293,7402,HePG2,PK15,ST,and TM3).MTT assays indicated that HvAV-3j inhibited the growth of host insect cells from the 6th hpi,but no effects were detected in the HvAV-3j-inoculated mammalian ***,viral DNA replication,gene transcription,and protein expression were investigated,and the results consistently suggested that HvAV-3j viruses were not able to replicate their genomic DNA,transcribe,or express their proteins in the non-target vertebrate *** HvAV-3j genes were only transcribed and expressed in the four insect cell *** results indicated that HvAV-3j was infectious to cells derived from ***,***,***,and *** but not to cells derived from human,pig,and mouse,suggesting that ascoviruses are safe to nontarget vertebrate cells.

关键词： Infectious risk Heliothis virescens ascovirus 3j(HvAV-3j) Mammalian cells Insect cells

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Genome analysis of Heliothis virescens ascovirus 3h isolated from China

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Virologica Sinica 2017年第2期32卷 147-154页

作者： guo-hua huang Dian-Hai Hou Manli Wang Xiao-Wen Cheng Zhihong Hu State Key Laboratory of Virology Microorganisms & Viruses Culture Collection CenterWuhan Institute of VirologyChinese Academy of Sciences Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Institute of VirologyHunan Agricultural University Department of Microbiology 32 Pearson HallMiami University

No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h （HvAV-3h） using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G＋C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames （ORFs） along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus la （SfAV-la）, Trichoplusia ni ascovirus 6a （TnAV-6a）, and Diadromus pulchellus ascovirus 4a （DpAV-4a）. Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except DpAV-4a. In the HvAV-3h genome, 24 baculovirus repeat ORFs （bros） were present, and the typical homologous repeat regions （hrs） were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.

关键词： Heliothis virescens ascovirus 3h (HvAV-3h) genome organization bro genes phylogenetic relationship

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3H-31, A Non-structural Protein of Heliothis virescens ascovirus 3h,Inhibits the Host Larval Cathepsin and Chitinase Activities

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Virologica Sinica 2021年第5期36卷 1036-1051页

作者： huan Yu Yi-Yi Ou-Yang Chang-Jin Yang Ni Li Madoka Nakai guo-hua huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangsha 410128China College of Plant Protection Hunan Agricultural UniversityChangsha 410128China Tokyo University of Agriculture and Technology SaiwaiFuchuTokyo 183-8509Japan

3h-31 of Heliothis virescens ascovirus 3h(Hv AV-3h)is a highly conserved gene of *** an early gene of Hv AV-3h,3h-31 codes for a non-structural protein(3H-31)of Hv *** the study,3h-31 was initially transcribed and expressed at 3 h post-infection(hpi)in the infected Spodoptera exigua fat body cells(Se FB).3h-31 was further inserted into the bacmid of Autographa californica nucleopolyhedrovirus(Ac MNPV)to generate an infectious baculovirus(Ac MNPV-31).In vivo experiments showed that budded virus production and viral DNA replication decreased with the expression of 3H-31,and lucent tubular structures were found around the virogenic stroma in the Ac MNPV-31-infected Se FB *** vivo,both LD50and LD90values of Ac MNPV-31 were significantly higher than those of the wild-type Ac MNPV(Ac MNPV-wt)in third instar *** *** interesting finding was that the liquefaction of the larvae killed by the infection of Ac MNPV-31 was *** and cathepsin activities of Ac MNPV-31-infected larvae were significantly lower than those of Ac MNPV-wt-infected *** possible regulatory function of the chitinase and cathepsin for 3H-31 was further confirmed by RNAi,which showed that larval cathepsin activity was significantly upregulated,but chitinase activity was not significantly changed due to the RNAi of *** on the obtained results,we assumed that the function of 3H-31 was associated with the inhibition of host larval chitinase and cathepsin activities,so as to restrain the hosts in their larval stages.

关键词： Cathepsin Chitinase Heliothis virescens ascovirus 3h(HvAV-3h) Recombinant virus 3h-31

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Satellite monitoring of land-use and land-cover changes in northern Togo protected areas

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Journal of Forestry Research 2014年第2期25卷 385-392页

作者： Fousseni Folega Chun-yu Zhang Xiu-hai Zhao Kperkouma Wala Komlan Batawila hua-guo huang Marra Dourma Koffi Akpagana The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University Université de Lomé Laboratoire de botanique et écologie végétaleFaculté des sciences

Remote-sensing data for protected areas in northern Togo, obtained in three different years （2007, 2000, and 1987）, were used to assess and map changes in land cover and land use for this drought prone zone. The normalized difference vegetation index （NDVI） was applied to the images to map changes in vegetation. An unsupervised classification, followed by classes recoding, filtering, identifications, area computing and post-classification process were applied to the composite of the three years of NDVI images. Maximum likelihood classification was applied to the 2007 image （ETM＋2007） using a supervised classification process. Seven vegetation classes were defined from training data sets. The seven classes included the following biomes： riparian forest, dry forest, flooded vegetation, wooded savanna, fallows, parkland, and water. For these classes, the overall accuracy and the overall kappa statistic for the classi- fied map were 72.5% and 0.67, respectively. Data analyses indicated a great change in land resources; especially between 1987 and 2000 proba- bly due to the impact of democratization process social, economic, and political disorder from 1990. Wide-scale loss of vegetation occurred during this period. However, areas of vegetation clearing and regrowth were more visible between 2000 and 2007. The main source of confusion in the contingency matrix was due to heterogeneity within certain classes. It could also be due to spectral homogeneity among the classes. This research provides a baseline for future ecological landscape research and for the next management program in the area.

关键词： land change NDVI land cover protected areas Northern Togo

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Novel strategies for the biocontrol of noctuid pests(Lepidoptera)based on improving ascovirus infectivity using Bacillus thuringiensis

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Insect Science 2021年第5期28卷 1452-1467页

作者： huan Yu Chang-Jin Yang Ni Li Ying Zhao Zhuang-Mei Chen Si-Jia Yi Zi-Qi Li Michael J.Adang guo-hua huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaHunan410128China College of Plant Protection Hunan Agricultural UniversityChangshaHunan410128China Department of Entomology University of GeorgiaAthensGA30602USA

ldentifying novel biocontrol agents and developing new strategies are urgent goals in insect pest *** are potential competent insect viruses that may be developed into bioinsecticides,but this aim is impeded by their poor oral *** improve the per os infectivity of ascovirus,Bacillus thuringiensis kurstaki(Btk)was employed as a helper to damage the midgut of lepidopteran larvae(Helicoverpa armigera,Mythimna separata,Spodoptera frugiperda,and ***)in formulations with Heliothis virescens ascovirus isolates(HvAV-3h and HvAV-3j).Btk and ascovirus mixtures(Btk/HvAV-3h and B1k/HvAV-3j)were fed to insect larvae(3rd instar).With the exception of *** larvae,which exhibited low mortality after ingesting Btk,the larvae of the other tested species showed three types of response to feeding on the formulas:type I,the tested larvae(***)were killed by Btk infection so quickly that insufficient time and resources remained for ascoviral invasion;type II,both Btk and the ascovirus were depleted by their competition,such that neither was successfully released or colonized the tissue;type II,Btk was eliminated by the ascovirus,and the ascovirus achieved systemic infection in the tested *** feeding of Btk/ascovirus formulas led to a great reduction in larval diet consumption and resulted in a significant decrease in the emergence rate of ***,***,and *** larvae,which suggested that the formulas exerted marked oral control effects on both the contemporary individuals and the next generation of these tested pest species.

关键词： ascovirus Bacillus thuringiensis formulation Heliothis virescens ascovirus 3h(HvAV-3h) Heliothis virescens ascovirus 3j(HvAV-3j) per os infectivity

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu guo Lanyue Zhang Gening Du Hao You Junfeng huang Mudong Tu hua Li Yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： NiFe-MIL-53 Rh Electrochemical reconstruction Catalytic mechanism Oxygen evolution reaction

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