Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations

作     者：Huan Yu Zi-Qi Li Yi-Yi Ou-Yang Guo-Hua Huang Huan Yu;Zi-Qi Li;Yi-Yi Ou-Yang;Guo-Hua Huang

作者机构：Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect PestsHunan Agricultural UniversityChangshaChina College of Plant ProtectionHunan Agricultural UniversityChangshaChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第6期

页      面：1158-1172页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：the National Natural Science Foundation of China(31700141,31872027) Changsha Science and Technology Plan Project(kq 1901033) Double first-class construction project of Hunan Agricultural University 

主　　题：apoptosis apoptotic inducing insect caspase SeCasp Spodoptera exigua 

摘      要：Apoptosis plays critical roles in multiple biological processes in multicellular *** are known as important participators and regulators of ***,four novel caspase genes of Spodoptera exigua were cloned and characterized which were designated as SeCasp-1,SeCasp-6,ScCasp-7 and *** of the putative encoded protein sequences of these SeCasps indicated that SeCasp-1 and ScCasp-7 were possible homologs of executor caspases;SeCasp-8 was a possible homolog of initiator caspases;and SeCasp-6 was a unique caspase of *** that shares low similarity with all the identified insect *** on baculovirus expression system analyses,SeCasp-1 exhibited similar caspase activity to human caspase-1,-3,-4,-6,-8 and-9;SeCasp-6 presented similar caspase activity to human caspase-2,-3,-4,-6,-8 and-9;SeCasp-7 exhibited similar caspase activity to human caspase-2,-3 and-6;and SeCasp-8 presented similar caspase activity only to human *** with diiTcrent chemicals revealed that SeCasp-1 showed extreme upregulation after 24 h in the treated fat body cell line(IOZCAS-Spex-II)of *** expression analysis revealed that SeCasp-1 was highly transcribed in the larval stages,while SeCasp-6,SeCasp-7,SeCasp-H were *** in vivo detection of the relative expression levels of SeCasps in *** larvae inoculated with different pathogens suggested that SeCasp-J was sensitive to Bacillus thuringiensis infection and that SeCasp-6 was sensitive to baculovirus ***-7 and SeCasp-H showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection.