In Vitro Infectious Risk Assessment of Heliothis virescens ascovirus 3j(HvAV-3j) toward Non-target Vertebrate Cells

作     者：Huan Yu Yi-Yi Ou-Yang Ni Li Madoka Nakai Guo-Hua Huang 

作者机构：Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect PestsHunan Agricultural UniversityChangsha 41012& China College of Plant ProtectionHunan Agricultural UniversityChangsha 41012& China Tokyo University of Agriculture and TechnologySaiwaiFuchuTokyo 183-8509Japan 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2019年第34卷第4期

页      面：423-433页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China (31700141, 31872027) the grant of Chinese Post-doctoral special funding (2018T110832) Program to supporting research activities of female researchers in Ministry of education, culture, sports, science and technology-Japan 

主　　题：Infectious risk Heliothis virescens ascovirus 3j(HvAV-3j) Mammalian cells Insect cells 

摘      要：As specific pathogens of noctuid pests,including Spodoptera exigua,***,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of *** this study,the infectivity of Heliothis virescens ascovirus 3j(HvAV-3j)on insect and mammalian cells was ***-3j infection induced drastic morphological changes in Sf9,HzAMl,SeFB,and HaFB cells,including swelling and detachmen Notably,the latter phenomena did not occur in HvAV-3j-inoculated mammalian cells(HEK293,7402,HePG2,PK15,ST,and TM3).MTT assays indicated that HvAV-3j inhibited the growth of host insect cells from the 6th hpi,but no effects were detected in the HvAV-3j-inoculated mammalian ***,viral DNA replication,gene transcription,and protein expression were investigated,and the results consistently suggested that HvAV-3j viruses were not able to replicate their genomic DNA,transcribe,or express their proteins in the non-target vertebrate *** HvAV-3j genes were only transcribed and expressed in the four insect cell *** results indicated that HvAV-3j was infectious to cells derived from ***,***,***,and *** but not to cells derived from human,pig,and mouse,suggesting that ascoviruses are safe to nontarget vertebrate cells.