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HT-SuperSAGE of the gut tissue of a Vip3Aa-resistant heliothis virescens (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance

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Insect Science 2019年第3期26卷 479-498页

作者： Camilo Ayra-Pardo Maria E. Ochagavia Ben Raymond Asim Gulzar Lianet Rodriguez-Cabrera Claudia Rodriguez de la Noval Ivis Moran Bertot Ryohei Terauchi Kentaro Yoshida Hideo Matsumura Pilar Tellez Rodriguez Daily Hernandez Hemandez Orlando Borras-Hidalgo Denis J. Wright Plant Division Centre for Genetic Engineering and Biotechnology (CIGB) Havana Cuba Department of Life Sciences Faculty of Natural Sciences Imperial College London Berkshire UK Genetics and Genomics Research Group Iwate Biotechnology Research Center Kitakami Japan Current addresses: University of Exeter Penryn campus Penryn Cornwall TR10 9FE UK Department of Entomology University of Arid Agriculture Rawalpindi Muree Road Rawalpindi 46300 Pakistan Laboratory of Plant Genetics Kobe University 1-1 Rokkodai-cho Nada ward Kobe 657-8501 Japan

Multitoxin Bt-crops expressing insecticidal toxins with different modes of action, for example, Cry and Vip, are expected to improve resistance management in target pests. While Cry1A resistance has been relatively well characterized in some insect species, this is not the case for Vip3A, for which no mechanism of resistance has yet been identified. Here we applied HT-SuperSAGE to analyze the transcriptome of the gut tissue of tobacco budworm heliothis virescens (F.) laboratory-selected for Vip3Aa resistance. From a total of 1 324 252 sequence reads, 5 895 126-bp tags were obtained representing 17 751 nonsingleton unique transcripts (UniTags) from genetically similar Vip3Aa-resistant (Vip- Sel) and susceptible control (Vip-Unsel) strains. Differential expression was significant (≥2.5 fold or ≤0.4;P < 0.05) for 1989 sequences (11.2% of total UniTags), where 420 represented overexpressed (OE) and 1569 underexpressed (UE) genes in Vip-Sel. BLASTN searches mapped 419 UniTags to H. virescens sequence contigs, of which, 416 (106 OE and 310 UE) were unambiguously annotated to proteins in NCBI nonredundant protein databases. Gene Ontology distributed 345 of annotated UniTags in 14 functional categories with metabolism (including serine-type hydrolases) and translation/ribosome biogenesis being the most prevalent. A UniTag homologous to a particular member of the REsponse to PAThogen (REPAT) family was found among most overexpressed, while UniTags related to the putative Vip3Aa-binding ribosomal protein S2 (RpS2) were underexpressed. qRT-PCR of a subset of UniTags validated the HT-SuperSAGE data. This study is the first providing lepidopteran gut transcriptome associated with Vip3Aa resistance and a foundation for future attempts to elucidate the resistance mechanism.

关键词： Bt resistance heliothis virescens HT-SuperSAGE transcriptomics Vip3Aa

Evaluating host plant resistance in cotton (Gossypium hirsutumL.) with varying gland densities to tobacco budworm (heliothis virescensF.) and bollworm (Helicoverpa zeaBoddie) in the field and laboratory

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Agricultural Sciences 2012年第1期3卷 14-23页

作者： Jodi A. Scheffler Gabriela B. Romano Carlos A. Blanco Crop Genetics Research Unit United States Department of Agriculture—Agricultural Research Service (USDA-ARS) Stoneville USA Southern Insect Management Research Unit USDA-ARS Stoneville USA

Cotton (Gossypium hirsutum L.) produces a number of toxic terpenoid aldehyde (TA) compounds contained in epidermal glands that help protect the plant from pests and diseases. In the seed, one of these toxic compounds, gossypol, limits the use of the seed to ruminants such as dairy cows. There are breeding techniques and germplasm available to decrease gossypol in the seed, but the breeding process also needs to include methods to evaluate the plant’s ability to resist insect pests. Three approaches were used to assess resistance of cotton to herbivory from bollworm (Helicoverpa zea Boddie) and tobacco budworm (heliothis virescens F.) including field counts, controlled field antibiosis assays and laboratory feeding tests of young field grown leaves. Results indicated that both field and laboratory evaluation could provide an assessment of the cotton host’s resistance. Measurements of terpenoid aldehydes (TAs) in the seed and the leaves, confirmed that the levels and types of TAs in the seed were not always good estimators of leaf TAs and that other TAs such as hemigossypolone and heliocides contribute to host plant resistance.

关键词： Cotton Gossypol Helicoverpa heliothis Terpenoid Aldehydes Host Plant Resistance

EFFECT OF BRAIN ON SEX PHEROMONE PRODUCTION OF THE COTTON BOLLWORM heliothis armigera H(?)BNER(LEPIDOPTERA: NOCTUIDAE)

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Chinese Science Bulletin 1988年第17期33卷 1474-1476页

作者：朱湘雄许少甫戴小杰杜家伟 Shanghai Institute of Entomology Academia Sinica

In the early 1970s, Riddiford and Williams found that removal of pupal corpora cardiaca prevented calling behaviour in the silkmoth Antheraea polyphemus and Hyalophora cecropia. However, the reinvestigation by Sasaki ... 详细信息

关键词： heliothis armigera sex pheromone brain factor

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拟除虫菊酯的应用研究 Ⅳ.拟除虫菊脂一杀虫脒合剂棉虫防治应用领域的研究—(2)合剂对棉铃虫(heliothis armigera Hübner)的田间增效试验初报

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中国农业大学学报 1984年第3期 285-291页

作者：傅凯廉孙庆玲曹本钧高洪杨国栋河北农业大学植保系北京农业大学土化系河北省香河县农林局

从害虫综合防治体系对药剂使用的基本原则要求,以及从更好地提高拟除虫菊酯类药剂的经济使用效益和节约能源等角度考虑,我们认为这类高效、低残毒的广谱系药剂应用中需要解决的重要问题是较大幅度降低施药剂量和减少施药次数。为解决这... 详细信息

从害虫综合防治体系对药剂使用的基本原则要求,以及从更好地提高拟除虫菊酯类药剂的经济使用效益和节约能源等角度考虑,我们认为这类高效、低残毒的广谱系药剂应用中需要解决的重要问题是较大幅度降低施药剂量和减少施药次数。为解决这些问题,我们从二代棉铃

关键词：棉铃虫类合棉铃实夜蛾棉花害虫 bner heliothis armigera 合剂田间

Resistance Analysis of the Binary Insect-resistant Transgenic Soybean to heliothis viriplaca

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Agricultural Science & Technology 2008年第3期9卷 67-69页

作者：杨向东郭东全包绍君赵桂兰康岭生钱雪燕尹爱萍邢国杰吉林省农业科学院生物技术研究中心吉林长春130124

[Objective] The aim of the research was to analyze the resistance of binary insect-resistant transgenic soybean to heliothis viriplaca.[Method]In this experiment, resistance analysis of the stabilized binary insect-resistant transgenic soybean to heliothis viriplaca was conducted in lab and in field conditions.[Result] The results indicated that the leaves of insect-resistant transgenic soybeans T5-150 and T5-195 showed lighter damage than those of non-transgenic soybeans. Meanwhile, the heliothis viriplaca larvae fed on leaves of these two transgenic soybeans were characterized by less leaf consumption, shortening survival day, slower development and less pupation.[Conclusion]It was concluded that insect-resistance of transgenic soybean to heliothis viriplaca was increased dramatically and the research provided a reference for selecting binary insect-resistant transgenic soybean to heliothis viriplaca.

关键词： heliothis viriplaca Transgenic soybean Insect resistance

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The role of herbivore- and plant-related experiences in intraspecific host preference of a relatively specialized parasitoid

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Insect Science 2019年第2期26卷 341-350页

作者： Tolulope Morawo Henry Fadamiro Department of Entomology & Plant Pathology Auburn University Auburn Alabama USA

Parasitoids use odor cues from infested plants and herbivore hosts to locate their hosts. Specialist parasitoids of generalist herbivores are predicted to rely more on herbivorederived cues than plant-derived cues. Microplitis croceipes (Cresson)(Hymenoptera: Braconidae) is a relatively specialized larval endoparasitoid of heliothis virescens (F.)(Lepidoptera: Noctuidae), which is a generalist herbivore on several crops including cotton and soybean. Using M. croceipes/H. virescens as a model system, we tested the following predictions about specialist parasitoids of generalist herbivores:(i) naive parasitoids will show innate responses to herbivore-emitted kairomones, regardless of host plant identity and (ii) herbivore-related experience will have a greater influence on intraspecific oviposition preference than plant-related experience. Inexperienced (naive) female M. croceipes did not discriminate between cotton-fed and soybean-fed H. virescens in oviposition choice tests, supporting our first prediction. Oviposition experience alone with either host group influenced subsequent oviposition preference while experience with infested plants alone did not elicit preference in M. croceipes, supporting our second prediction. Furthermore, associative learning of oviposition with host-damaged plants facilitated host location. I terestingly, naive parasitoids attacked more soybeathan cotton-fed host larvae in two-choice tests when a background of host-infested cotton odor was supplied, and vice versa. This suggests that plant volatiles may have created an olfactory contrast effect. We discussed ecological significance of the results and concluded that both plant- and herbivore-related experiences play important role in parasitoid host foraging.

关键词： heliothis virescens Microplitis croceipes olfactory contrast oviposition choice plant volatiles