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Frontiers of Agricultural Science and Engineering 2021年第2期8卷 236-246页

作者： Tonglu WEI dalong guo Jihong LIU Key Laboratory of Horticultural Plant Biology(MOE) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China College of Forestry Henan University of Science and TechnologyLuoyang 471023China.

Late embryogenesis abundant(LEA)genes encode highly hydrophilic proteins that are essential in abiotic stress ***,most LEA genes in higher plants have not yet been *** study identified an LEA family gene(PtrLEA7)from Poncirus trifoliata and studied its function in drought *** full-length coding sequence of PtrLEA7 was 420 bp encoding a protein of 139 amino *** analysis shows that PtrLEA7 protein belongs to the LEA_4 *** profiling by qPCR found that PtrLEA7 was strongly induced by dehydration,cold and ABA treatments,and slightly induced by salt *** localization reveals that PtrLEA7 protein was located in both cytoplasm and *** investigate its function,transgenic plants of both tobacco and Poncirus trifoliata overexpressing PtrLEA7 were *** tolerance assays show that overexpression lines had enhanced dehydration and drought tolerance compared with wild type plants,indicating that PtrLEA7 positively regulates drought *** addition,transgenic plants had much higher expression levels of three antioxidant enzyme genes(CAT,SOD and POD)and significantly increased catalase enzyme activity,accompanied by reduced reactive oxygen species accumulation in comparison with wild type ***,this study demonstrates that PtrLEA7 can confer enhanced drought tolerance partially via enhancing antioxidant capacity.

关键词： abiotic stress antioxidant drought late embryogenesis abundant Poncirus trifoliata

Integrated analysis of transcriptomic and proteomic data from tree peony(***)seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

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Horticulture Research 2019年第1期6卷 344-362页

作者： Xiaojing Wang Haiying Liang dalong guo Lili guo Xiangguang Duan Qishi Jia Xiaogai Hou College of Agriculture/College of Tree Peony Henan University of Science and TechnologyLuoyang 471023China Department of Genetics and Biochemistry Clemson UniversityClemsonSC 29634-0318USA College of Forestry Henan University of Science and TechnologyLuoyang 471023China

Tree peony(Paeonia section Moutan DC.)seeds are an excellent source of beneficial natural compounds that promote health,and they contain high levels of alpha-linolenic acid(ALA).In recent years,tree peony has been emerging as an oil ***,combined analysis of the transcriptome and proteome of tree peony(***)seeds at 25,32,39,53,67,81,88,95,and 109 days after pollination(DAP)was conducted to better understand the transcriptional and translational regulation of seed development and oil biosynthesis.A total of 38,482 unigenes and 2841 proteins were identified.A total of 26,912 differentially expressed genes(DEGs)and 592 differentially expressed proteins(DEPs)were clustered into three groups corresponding to the rapid growth,seed inclusion enrichment and conversion,and late dehydration and mature stages of seed *** lipid metabolism pathways were identified at both the transcriptome and proteome *** enrichment analysis revealed that a period of rapid fatty acid biosynthesis occurred at 53–88 ***,211 genes and 35 proteins associated with the fatty acid metabolism pathway,63 genes and 11 proteins associated with the biosynthesis of unsaturated fatty acids(UFAs),and 115 genes and 24 proteins associated with ALA metabolism were *** analysis revealed that 16 putative fatty acid desaturase(FAD)-encoding genes clustered into four FAD groups,eight of which exhibited the highest expression at 53 DAP,suggesting that they play an important role in ALA ***-qPCR analysis indicated that the temporal expression patterns of oil biosynthesis genes were largely similar to the RNA-seq *** expression patterns of fatty acid metabolism-and seed development-related proteins determined by MRM were also highly consistent with the results obtained in the proteomic *** analysis indicated significant differences in the number and abundance of DEGs and DEPs but a high level of consistency in

关键词： tree analysis consistency

Genome-wide association study of 23 f lowering phenology traits and 4 f loral agronomic traits in tree peony (Paeonia section Moutan DC.) reveals five genes known to regulate f lowering time

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Horticulture Research 2023年第2期10卷 118-133页

作者： Yuying Li Lili guo Zhanying Wang Dehui Zhao dalong guo John E.Carlson Weilun Yin Xiaogai Hou College of Agronomy/College of Tree Peony Henan University of Science and TechnologyLuoyangHenan471023China Luoyang Academy of Agricultural and Forestry Sciences LuoyangHenan471000China College of Forestry Henan University of Science and TechnologyLuoyangHenan471023China Department of Ecosystem Science and Management Pennsylvania State UniversityUniversity ParkPA 16802USA College of Biological Sciences and Technology Beijing Forestry UniversityBeijing 100083China

Tree peony is a unique traditional f lower in China,with large,fragrant,and colorful f ***,a relatively short and concentrated f lowering period limits the applications and production of tree peony.A genome-wide association study(GWAS)was conducted to accelerate molecular breeding for the improvement of f lowering phenology traits and ornamental phenotypes in tree peony.A diverse panel of 451 tree peony accessions was phenotyped for 23 f lowering phenology traits and 4 f loral agronomic traits over 3 *** by sequencing(GBS)was used to obtain a large number of genome-wide single-nucleotide polymorphisms(SNPs)(107050)for the panel genotypes,and 1047 candidate genes were identified by association ***-two related genes were observed during at least 2 years for f lowering,and seven SNPs repeatedly identified for multiple f lowering phenology traits over multiple years were highly significantly associated with five genes known to regulate f lowering *** validated the temporal expression profiles of these candidate genes and highlighted their possible roles in the regulation of f lower bud differentiation and f lowering time in tree *** study shows that GWAS based on GBS can be used to identify the genetic determinants of complex traits in tree *** results expand our understanding of f lowering time control in perennial woody *** of markers closely related to these f lowering phenology traits can be used in tree peony breeding programs for important agronomic traits.

关键词： breeding traits tree

Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

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Journal of Integrative Agriculture 2024年第3期23卷 863-875页

作者： Hainan Liu Maosong Pei Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi dalong guo College of Horticulture and Plant Protection Henan University of Science and TechnologyLuoyang 471000China Henan Engineering Technology Research Center of Quality Regulation and Control of Horticultural Plants Luoyang 471000China The New Zealand Institute for Plant&Food Research Limited(PFR) Auckland 1010New Zealand

The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated *** study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

关键词： grape postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)

Identification,characterization,and verification of miR399 target gene in grape

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Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai dalong guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

Creation of a watermelon haploid inducer line via ClDMP3-mediated single fertilization of the central cell

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Horticulture Research 2023年第6期10卷 312-321页

作者： Xiner Chen Yuxiu Li Gongli Ai Jinfan Chen dalong guo Zhonghou Zhu Xuejie Zhu Shujuan Tian Jiafa Wang Man Liu Li Yuan State Key Laboratory of Crop Stress Biology for Arid Areas College of HorticultureNorthwest A&F UniversityYangling712100ShanxiChina College of Horticulture and Plant Protection Henan University of Science and Technology 471000LuoyangChina Luoyang Nongfa Agricultural Technology Co. LTD471100LuoyangChina Shenzhen Research Institute Northwest A&F UniversityShenzhen518000GuangdongChina

The use of doubled haploids is one of the most efficient breeding methods in modern *** of pollen grains has been shown to induce haploids in cucurbit crops,possibly because it causes preferential fertilization of the central cell over the egg *** of the DMP gene is known to induce single fertilization of the central cell,which can lead to the formation of *** the present study,a detailed method of creating a watermelon haploid inducer line via ClDMP3 mutation is *** cldmp3 mutant induced haploids in multiple watermelon genotypes at rates of up to 1.12%.These haploids were confirmed via fluorescent markers,flow cytometry,molecular markers,and *** haploid inducer created by this method has the potential to greatly advance watermelon breeding in the future.

关键词： breeding crops fir

Atomic structure and collision dynamics with highly charged ions

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Chinese Physics B 2022年第9期31卷 13-40页

作者： Xinwen Ma Shaofeng Zhang Weiqiang Wen Zhongkui Huang Zhimin Hu dalong guo Junwen Gao Bennaceur Najjari Shenyue Xu Shuncheng Yan Ke Yao Ruitian Zhang Yong Gao Xiaolong Zhu Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Atomic and Molecular Physics and Functional Materials of Gansu Province College of Physics and Electronic EngineeringNorthwest Normal UniversityLanzhou 730070China Key Laboratory of Radiation Physics and Technology of the Ministry of Education Sichuan UniversityChengdu 610064China Department of Physics Hangzhou Normal UniversityHangzhou 311121China Shanghai EBIT Laboratory Key Laboratory of Nuclear Physics and Ion-Beam Application(MOE)Fudan UniversityShanghai 200433China

The research progresses on the investigations of atomic structure and collision dynamics with highly charged ions based on the heavy ion storage rings and electron ion beam traps in recent 20 years are *** structure part covers test of quantum electrodynamics and electron correlation in strong Coulomb field studied through dielectronic recombi-nation spectroscopy and VUV/x-ray *** collision dynamics part includes charge exchange dynamics in ion-atom collisions mainly in Bohr velocity region,ion-induced fragmentation mechanisms of molecules,hydrogen-bound and van de Waals bound clusters,interference,and phase information observed in ion-atom/molecule *** this achievements,two aspects of theoretical studies related to low energy and relativistic energy collisions are *** applications of data relevant to key atomic processes like dielectronic recombination and charge exchanges involving highly charged ions are *** the end of this review,some future prospects of research related to highly charged ions are proposed.

关键词： highly charged ion atomic structure collision dynamics quantum electrodynamics fragmentation mechanisms relativistic effects electron correlation

Geometric structure of N_(2)O^(q+)(q=5,6)studied by Ne^(8+)ion-induced Coulomb explosion imaging

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Chinese Physics B 2021年第11期30卷 309-317页

作者： Xi Zhao Xu Shan Xiaolong Zhu Lei Chen Zhenjie Shen Wentian Feng dalong guo Dongmei Zhao Ruitian Zhang Yong Gao Zhongkui Huang Shaofeng Zhang Xinwen Ma Xiangjun Chen Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China

We report the study on the complete three-body Coulomb explosion(CE)of N_(2)O^(q+)(q=5,6)induced by 56-keV/u Ne8+ion collision with N2O gaseous *** CE channels for N_(2)O^(5+)and seven for N_(2)O^(6+)are identified by measuring three ionic fragments and the charge-changed projectile in quadruple *** the kinetic energy release(KER)and momentum correlation angle(MCA)distributions of three ionic fragments for each of the CE channels are also *** computation is presented to reconstruct the geometric structure of N_(2)O^(q+0prior to dissociation based on the measured KER and *** N–N and N–O bond lengths and the N–N–O bond angles of N_(2)O^(q+)for each of the channels are determined.

关键词： Coulomb explosion kinetic energy release molecular geometry ion collision nitrous oxide(N_(2)O)

油用凤丹牡丹不同种植时间根际细菌群落多样性变化

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林业科学 2017年第11期53卷 131-141页

作者：郭丽丽尹伟伦郭大龙侯小改河南科技大学农学院洛阳471023 北京林业大学生物科学与技术学院北京100083

【目的】土壤微生物在林业生态系统中具有重要的功能,解析油用凤丹牡丹长期人工种植后根际土壤中细菌群落结构多样性变化情况,为揭示油用凤丹牡丹长期连作后根际病害抑制性土壤形成的机制奠定基础。【方法】试验采集种植2、4、5、10和3... 详细信息

【目的】土壤微生物在林业生态系统中具有重要的功能,解析油用凤丹牡丹长期人工种植后根际土壤中细菌群落结构多样性变化情况,为揭示油用凤丹牡丹长期连作后根际病害抑制性土壤形成的机制奠定基础。【方法】试验采集种植2、4、5、10和32年生凤丹牡丹的根际土壤,应用Illumina Mi Seq高通量测序技术,分析土壤细菌16S rRNA基因V3+V4区域的丰富度和多样性指数,研究连作对凤丹牡丹根际细菌群落结构及多样性的影响。【结果】源于不同种植时间(年限)的15个根际土壤样本共获得2 366个涵盖24门、79纲、113目、117科、103属的OTUs。凤丹牡丹根际土壤中心优势菌群为:变形菌门(34%)、酸杆菌门(14%)、浮霉菌门(14%)和放线菌门(10%)等。纲层次上的优势菌群为:变形菌门的δ-变形菌纲(26%)、α-变形杆菌纲(25%)、β-变形菌纲(15%)和γ-变形菌纲(15%);酸杆菌门的酸杆菌纲(44%)和Solibacteres(14%);浮霉菌门的浮霉菌纲(27%)和Planctomycetia(60%);放线菌门的放线菌纲(25%)、酸微菌纲(18%)、嗜热油菌纲(17%)、MB-A2-108(15%)、红杆菌纲(10%)。【结论】不同种植年限凤丹牡丹土壤中细菌优势菌群组成结构变化较小,但菌群多样性呈下降趋势;不同种植年限的土壤具有特异性、高丰度和低丰度细菌种属。随种植年限延长,酸杆菌门等细菌群落逐年积累,绿菌门和纤维杆菌门等特异性菌群出现,放线菌门、变形菌门、浮霉菌门等菌群丰度逐年降低,疣微菌门等菌群消失的现象可能是造成多年连续单一种植凤丹牡丹土壤细菌选择性抑制生长以及土壤病害发生、土壤退化的重要原因之一。凤丹牡丹根际微生物对维持根际土壤微环境具有重要的生态学意义。

关键词：凤丹牡丹种植年限根际土壤 Illumina高通量测序细菌多样性

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双牙棒消隙C轴箱的结构设计

制造技术与机床 2017年第8期 64-65页

作者：崔大龙郭婧一重集团绍兴重型机床有限公司黑龙江齐齐哈尔161005 黑龙江省完达山乳液股份有限公司黑龙江齐齐哈尔161000

在重型立式车铣加工中心的铣削与钻削加工中,对圆周进给和精确的分度定位要求越来越高,为满足上述要求,开发研制了双牙棒消隙的C轴箱,采用液压缸推动两个旋向相反的斜齿轮,使之产生轴向位移,进而消除传动链中间隙。

关键词： C轴箱双牙棒消隙液压缸轴向位移