Creation of a watermelon haploid inducer line via ClDMP3-mediated single fertilization of the central cell

作     者：Xiner Chen Yuxiu Li Gongli Ai Jinfan Chen Dalong Guo Zhonghou Zhu Xuejie Zhu Shujuan Tian Jiafa Wang Man Liu Li Yuan Xiner Chen;Yuxiu Li;Gongli Ai;Jinfan Chen;Dalong Guo;Zhonghou Zhu;Xuejie Zhu;Shujuan Tian;Jiafa Wang;Man Liu;Li Yuan

作者机构：State Key Laboratory of Crop Stress Biology for Arid AreasCollege of HorticultureNorthwest A&F UniversityYangling712100ShanxiChina College of Horticulture and Plant Protection Henan University of Science and Technology471000LuoyangChina Luoyang Nongfa Agricultural Technology Co.LTD471100LuoyangChina Shenzhen Research InstituteNorthwest A&F UniversityShenzhen518000GuangdongChina 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2023年第10卷第6期

页      面：312-321页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Youth Talent Program Earmarked Fund for China Agriculture Research System Key-Area R&D Program of Guangdong Province Key R&D Program of Shaanxi province 陕西省自然科学基金 the Science and Technology Innovation Team of Shaanxi 

主　　题：breeding crops fir 

摘      要：The use of doubled haploids is one of the most efficient breeding methods in modern *** of pollen grains has been shown to induce haploids in cucurbit crops,possibly because it causes preferential fertilization of the central cell over the egg *** of the DMP gene is known to induce single fertilization of the central cell,which can lead to the formation of *** the present study,a detailed method of creating a watermelon haploid inducer line via ClDMP3 mutation is *** cldmp3 mutant induced haploids in multiple watermelon genotypes at rates of up to 1.12%.These haploids were confirmed via fluorescent markers,flow cytometry,molecular markers,and *** haploid inducer created by this method has the potential to greatly advance watermelon breeding in the future.