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The Crop Journal 2022年第4期10卷 1006-1013页

作者： Lu Lu Hui Liu Yu Wu guijun yan UWA School of Agriculture and Environment The University of Western AustraliaPerthWA 6009Australia The UWA Institute of Agriculture The University of Western AustraliaPerthWA 6009Australia Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041SichuanChina

Wheat production is seriously influenced by extreme hot weather,which has attracted increasing *** is important to compare wheat responses to heat at seedling and reproductive stages,to explore the potential relationship between the performances at different growing stages and the possibility of early selection to accelerate heat tolerance *** this study,forty wheat genotypes were screened under heat stress at both seedling and adult *** was found that root lengths at seedling stage were severely reduced by heat stress with significant variations among wheat ***-tolerant genotypes at seedling stage showed less root length decrease than susceptible *** genotypes tolerant at seedling stage showed higher yield at adult stage after heat *** performances of wheat genotypes screened under heat stress at seedling and adult stages were ranked by seedling damage index and adult damage index.A significant positive relationship was found between heat tolerance at seedling stage and at adult stage(r=0.6930),suggesting a similar tolerant/susceptible mechanism at different plant growth stages and the possibility of early selection at seedling stage for breeding heat *** tolerant and susceptible genotypes with consistent performances at seedling and adult stages were genetically compared and associated SNP markers and linked candidate genes were identified.

关键词： Heat tolerance Seedling stage Adult stage Linear regression Genetic mechanism

Identification of KASP markers and putative genes for pre-harvest sprouting resistance in common wheat(Triticum aestivum L.)

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The Crop Journal 2023年第2期11卷 549-557页

作者： Guannan Liu Daniel Mullan Aimin Zhang Hui Liu Dongcheng Liu guijun yan UWA School of Agriculture and Environment and The UWA Institute of AgricultureThe University of Western AustraliaPerthWA 6009Australia InterGrain Pty.Ltd. 19 Ambitious Link Bibra LakeWA 6163Australia State Key Laboratory of North China Crop Improvement and Regulation College of AgronomyHebei Agriculture UniversityBaoding 071000HebeiChina

Common wheat(Triticum aestivum L.)is the most important crop in the world and a typical allopolyploid with a large and complex ***-harvest sprouting(PHS)leads to a significant reduction in grain quality *** is a complex trait with related QTL located on different ***,the study of markers and genes related to PHS resistance is limited especially for whitegrained *** pairs of near isogenic lines(NILs)from a white-grained wheat cross of CharaDM5637B*8 targeting a major QTL for PHS resistance(***-3A.1)on wheat chromosme 3AL were genotyped using the 90K SNP Illumina iSelect *** SNPs were identified,with a 75%-100%consistency between genotype and phenotype in the resistant or susceptible *** 10 SNPs were converted to cost-effective kompetitive allele-specific PCR(KASP)*** of 48 wheat cultivars with different phenotypes of PHS identified four KASP markers with 81.3%-85.4%conformity between genotype and *** investigation revealed that the four SNPs(BS00022245_51,Kukri_c49927_151,BS00022884_51 and BS00110550_51)corresponding to the four validated KASP markers are residing in three independent genes(TraesCS3A03G1072800,TraesCS3A03G1072400,TraesCS3A03G1071800)close to each other with a distance of 4.28-4.48 Mb to the targeted *** three annotated genes have potential functions related to PHS *** study revealed that combined use of NILs and the 90K SNP chip is a powerful approach for developing KASP markers and mining functional genes in *** KASP markers for PHS resistance on chromosome 3AL are useful for high-throughput evaluation and marker-assisted selection,and the three identified genes could lead to a better understanding of the genetic pathways controlling PHS.

关键词： Pre-harvest sprouting(PHS) KASP marker Functional genes Chromosome 3AL 90K SNP assay Near-isogenic lines

Pravastatin alleviates lipopolysaccharide-induced placental TLR4 over-activation and promotes uterine arteriole remodeling without impairing rat fetal development

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The Journal of Biomedical Research 2018年第4期32卷 288-297页

作者： Muyi yang Zhenyu Diao Zhiyin Wang guijun yan Guangfeng Zhao Mingming Zheng Anyi Dai Yimin Dai Yali Hu Drum Tower Clinical Medical College Nanjing Medical University Department of Obstetrics and Gynecology Nanjing Drum Tower HospitalNanjing University Medical School

Preeclampsia is associated with over-activation of the innate immune system in the placenta,in which toll-like receptor 4（TLR4） plays an essential *** their potent anti-inflammatory effects,statins have been suggested as potential prevention or treatment of preeclampsia,although evidence remains ***,we investigated whether pravastatin could ameliorate preeclampsia-like phenotypes in a previously established lipopolysaccharide（LPS）-induced rat preeclampsia model,through targeting the TLR4/NF-κB *** results showed that pravastatin reduced the blood pressure [maximum decline on gestational day（GD） 12,（101.33±2.49） mmHg vs.（118.3±1.37） mmHg,P〈0.05] and urine protein level [maximum decline on GD9,（3,726.23± 1,572.86） μg vs.（1,991.03 ±609.37）μg,P〈 0.05],which were elevated following LPS *** also significantly reduced the rate of fetal growth restriction in LPS-treated rats（34.10% vs.8.99%,P〈0.05）.Further pathological analyses suggested a restoration of normal spiral artery remodeling in preeclampsia rats by pravastatin *** effects of pravastatin were associated with decreased TLR4/NF-κB protein levels in the placenta and IL-6/MCP-1 levels in ***,no obvious abnormalities in fetal liver,brain,and kidney were found after administration of *** results provide supportive evidence for use of pravastatin in preventing preeclampsia.

关键词： preeclampsia arteriole remodeling pravastatin toll-like receptor 4 fetal development

Umbilical cord artery-derived perivascular stem cells for treatment of ovarian failure through CD146 signaling

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2694-2697页

作者： Lu Xu yanjun yang Lingling Zhang guijun yan Shiyuan Li Yifan Li Yali Hu Lijun Ding Bruno Péault Haixiang Sun Center for Reproductive Medicine and Obstetrics and Gynecology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Center for Molecular Reproductive Medicine Nanjing UniversityNanjing 210093China Department of Obstetrics and Gynecology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China NHC Key Lab of Reproduction Regulation Shanghai Institute of Planned Parenthood ResearchPharmacy SchoolFudan UniversityShanghai 200032China Clinical Center for Stem Cell Research the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Orthopedic Hospital Research Center and Broad Stem Cell Center David Geffen School of MedicineUniversity of CaliforniaLos AngelesCAUSA State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjing 210008China

Dear Editor,Chemotherapy-induced ovarian failure significantly diminishes ovarian blood flow,ovarian size,and follicular *** plays a vital role in repairing ovarian damage.1 Perivascular stem cells(PSCs),known as mural cells covering the vasculature,are essential for blood vessel formation and postulated as progenitors of mesenchymal stem cells(MSCs).2,3 We previously established umbilical cord artery-derived PSCs(UCA-PSCs)and Wharton’s jelly-derived MSCs(WJ-MSCs)and UCAPSCs display optimal angiogenic capacity in vitro.4 Therefore,we explored the angiogenesis and pro-angiogenesis mechanisms of UCA-PSCs and provided them as an efficient treatment strategy for ovarian failure.

关键词： CD146 treatment artery damage

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油料作物育种的分子工具和技术创新

浙江大学学报（农业与生命科学版） 2023年第4期49卷 445-453页

作者：许玲 Hui LIU guijun yan Wallace COWLING 周伟军路战远浙江理工大学生命科学与医药学院浙江杭州310018 西澳大学农业与环境学院/西澳大学农业研究所西澳大利亚州珀斯6009 浙江大学农业与生物技术学院浙江杭州310058 内蒙古自治区农牧业科学院内蒙古呼和浩特010031

油料作物的主要育种目标是提高产量、品质和抗逆性。目前,就产量而言,全球主要油料作物依次为大豆、油菜、向日葵和花生。本文综述了这些主要油料作物育种的分子工具和技术创新,包括基因组选择、基因组编辑、分子设计育种等新技术,指出... 详细信息

油料作物的主要育种目标是提高产量、品质和抗逆性。目前,就产量而言,全球主要油料作物依次为大豆、油菜、向日葵和花生。本文综述了这些主要油料作物育种的分子工具和技术创新,包括基因组选择、基因组编辑、分子设计育种等新技术,指出了当前在遗传研究和育种实践中存在的主要问题,并对技术进展和未来应用前景进行了展望,旨在为油料作物高产、优质、高效育种提供借鉴。

关键词：油料作物育种分子工具技术创新

Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman＇s syndrome

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Science China(Life Sciences) 2017年第4期60卷 404-416页

作者： Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang guijun yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai yan Zhou Yali Hu Department of Obstetrics and Gynecology The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China Center for Reproductive Medicine Department of Obstetrics and Gynecology The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China Department of Pathology The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China Nanjing Center for Stem Cells and Biomaterials The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China Instituteof Genetics and Developmental Biology Chinese Academy of Sciences Beijung 100190 China Department of Obstetrics Gynecology and Reproductive Sciences Center for Reproductive SciencesEli and Edythe Broad Center of Regeneration Medicine and Stern Cell Research University of California San Francisco San Francisco 94143 USA

Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS.

关键词： endometrial scaffold collagen uterine alterations mononuclear regeneration impaired reconstruct autologous

Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility

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Science China(Life Sciences) 2018年第12期61卷 1554-1565页

作者： Lijun Ding guijun yan Bin Wang Lu Xu yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang yanjun yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun Center for Reproductive Medicine Department of Obstetrics and Gynecologythe Affiliated Drum Tower Hospital of Nanjing University Medieal SchoolNanjing 210008China Clinical Center for Stem Cell Research the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Obstetrics and Gynecology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China State Key Laboratory of Molecular Development Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences Beijing 100190China

Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF.

关键词： premature ovarian failure collagen scaffold UC-MSCs primordial follicle activation granulosa cells FOXO3a FOXO1

The neonatal ketone body is important for primordial follicle pool formation and regulates ovarian ageing in mice

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Life Metabolism 2022年第2期1卷 149-160页

作者： Xin-Ying Wang Xin-Ge Zhang Yong-Juan Sang Dan-yang Chong Xiao-Qiang Sheng Hai-Quan Wang Chao-Fan yang guijun yan Hai-Xiang Sun Chao-Jun Li State Key Laboratory of Reproductive Medicine and China International Joint Research Center on Environment and Human Health Center for Global HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Center for Reproductive Medicine Department of Obstetrics and GynecologyNanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjingJiangsu 210093China Ministry of Education Key Laboratory of Model Animal for Disease Study Model Animal Research Center of Nanjing UniversityNanjingJiangsu 210093China

Adverse nutritional conditions during the perinatal stage are related to early menopause in adulthood;however,the underlying mechanism is still ***,we revealed that colostrum-activated ketone body elevation during the postnatal stage regulated primordial follicle reservoir size and then affected ovarian *** found that the expression of the ketogenesis rate-limiting enzyme 3-hydroxy-3-methylglutaryl-CoA synthase 2(Hmgcs2)was largely enhanced during primordial follicle pool formation after birth and might be activated in the ovaries by *** oxygen species(ROS)elevation in the ovaries leads to follicle apoptosis to deplete damaged follicles,while Hmgcs2 deficiency enhances follicle apoptosis and thus decreases the size of the primordial follicle pool and leads to premature ovarian ageing(POA),which might be related to the activation of cellular endogenous antioxidant *** these defects could be rescued by ketone body administration,which suppressed ROS-activated follicle *** results suggest that the internal metabolic homeostasis of newborn mice is critical for the primordial reservoir and that any intrauterine and perinatal undernutrition could result in POA.

关键词： ketone body primordial follicle pool Hmgcs2 ROS POA

子宫内膜上皮细胞类器官在生殖领域的研究进展

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中国临床药理学与治疗学 2024年第5期29卷 576-582页

作者：曹志文颜桂军南京大学医学院附属鼓楼医院生殖医学和妇产医学中心江苏南京210000

近年来,子宫内膜上皮细胞类器官研究在生殖领域取得了显著进展。传统的二维细胞培养模型和动物实验难以准确还原子宫内膜的三维结构和生理功能,从而限制了对子宫内膜上皮细胞正常生理机制和相关疾病机制的深入研究。新兴的类器官技术为... 详细信息

近年来,子宫内膜上皮细胞类器官研究在生殖领域取得了显著进展。传统的二维细胞培养模型和动物实验难以准确还原子宫内膜的三维结构和生理功能,从而限制了对子宫内膜上皮细胞正常生理机制和相关疾病机制的深入研究。新兴的类器官技术为此提供了新途径,子宫内膜上皮细胞类器官通过干细胞或前体细胞在三维培养基中自行组织形成,成功地高度还原了在体子宫内膜腺体的特征。这种类器官模型不仅能够模拟子宫内膜上皮细胞在不同周期阶段的生理变化,还能够模拟囊胚与子宫内膜之间的复杂互动过程。此外,子宫内膜上皮细胞类器官系统为生殖领域的基础研究和临床研究提供了重要工具,包括生殖相关基础研究、疾病机制探索、药物筛选及靶向治疗研究等。这些研究可为深入了解子宫内膜的生物学特性、疾病机制以及相关疾病的治疗策略提供全新的思路和方法。

关键词：子宫内膜上皮细胞类器官药物筛选

A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom

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A novel fibrinolytic enzyme from Cordyceps militaris, a Chin...