检索结果-南通市图书馆

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer:a review

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第2期1卷 104-113页

作者： Fengxia YIN Hui liU Shorgan BOU guangpeng li The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology the College of Life SciencesInner Mongolia UniversityHohhot 010070China

Oocytes are unique cells with the inherent capability to reprogram *** reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear *** nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear *** chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm *** oocyte first obtains its reprogramming capability in the early fetal follicle,and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle *** cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular *** transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem *** maternal pronucleus exerts a predominant,transcriptiondependent effect on embryo cytofragmentation,with a lesser effect imposed by the ooplasm and the paternal *** deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.

关键词： nuclear reprogramming somatic cell transcription factors transcriptomics

Matrix attachment regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgenic cells and transgenic mice

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2016年第1期3卷 87-96页

作者： Xiaoming HU Jing GUO Chunling BAI Zhuying WEI li GAO Tingmao HU Shorgan BOU guangpeng li Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology/Key Laboratory of Herbivore Reproductive Biotechnology and Breeding of Ministry of Agriculture Inner Mongolia University

In the present study, follistatin(FST) gene expression vectors with either a bicistronic gene transfer cassette alone, or a bicistron gene cassette carrying a matrix attachment region(MAR) were constructed and transfected to bovine fetal fibroblasts. Evaluations of both the integration and expression of exogenous FST indicated that the p MAR-CAG-FST-IRES-Ac GFP1-poly A-MAR(pMAR-FST) vector had higher capacity to form monoclonal transgenic cells than the vector without MAR,though transient transfection and integration efficiency were similar with either construct. Remarkably, protein expression in transgenic cells with the p MAR-FST vector was significantly higher than that from the bicistronic vector. Exogenous FST was expressed in all of the p MARFST transgenic mice at F_0, F_1 and F_2. Total muscle growth in F_0 mice was significantly greater than in wild-type mice,with larger muscles in fore and hind limbs of transgenic mice. p MAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner spaces between sarcolemma, which suggests a correlation between transgene expression-associated muscle development and the trend of muscle growth. In conclusion, a p MAR-FST vector, which excluded the resistant genes and frame structure, enhances and stabilizes FST gene expressions in both transfected cells and transgenic mice.

关键词： safety of transgenic bicistron gene transfer body transgenic mice muscle development

In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第1期1卷 21-29页

作者： Guanghua SU Lei CHENG Yu GAO Kun liU Zhuying WEI Chunling BAI Fengxia YIN li GAO guangpeng li Shorgan BOU The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of AgricultureInner Mongolia UniversityHohhot 010021China

The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered *** a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was *** antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine *** cloned embryos were then cultured in vitro or in the oviducts of intermediate *** than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst ***,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst *** contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine *** treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and *** the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,*** is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be *** mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

关键词： interspecific nuclear transfer bovine ovine caprine oviduct apoptosis

Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第20期45卷 1883-1885页

作者： DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan SUN Qingyuan li guangpeng WANG Minkang liU Yilong KANG li HAN Zhiming SONG Xiangfen li Jingsong CHEN Yuchun Laboratory of Cellular and Molecular Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China Fuzhou Panda Institute Center Fuzhou 350001China 不详

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.

关键词： giant panda (Ailuropoda melanoleuca) interspecies cloning microsatellite DNA nucleus.

The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,China

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第3期1卷 191-200页

作者： Xinxin li Huijuan WANG Guanghua SU Zhuying WEI Chunling BAI Wuni-MENGHE Yanhui HOU Changqing YU Shorgan BOU guangpeng li The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding of Ministry of AgricultureInner Mongolia UniversityHohhot 010070China The Inner Mongolia Rangeland Ecology Institute Alashan 750306China

Since the birth of the first cloned sheep,somatic cell nuclear transfer technology has been successfully used to clone a variety of *** livestock have no apparent health risks,and the quality and safety of the cloned animal products are similar to non-cloned *** social behavior and environmental adaptability of postnatal cloned animals,especially when used for grassland farm production purposes,is *** the present study,the cloned Dorper sheep equipped with GPS location devices were free-grazed in a harsh natural environment similar to conditions commonly experienced by Mongolian *** main findings of this research were as follows.(1)Under free-grazing conditions,the cloned sheep showed excellent climatic and ecological *** extreme temperature conditions ranging from–30 to 40°C,the cloned sheep maintained acceptable body condition and behaved as other sheep.(2)The cloned sheep quickly adapted from a herd feeding strategy to the harsh environment and quickly exhibited a grazing regimen as other free-grazing sheep.(3)The cloned sheep exhibited free-grazing patterns and social behavior as other sheep.(4)The cloned sheep in the harsh environment thrived and produced healthy ***,the cloned Dorper sheep exhibited excellent ecological adaptation,which is an important consideration for breeding meat sheep by *** Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland,which attests to their ability to withstand harsh environmental conditions.

关键词： somatic cell nuclear transfer free-grazing synchronization Dorper sheep cloned animal ecology

在线全文

学校读者我要写书评

暂无评论

牛肉质性状及其影响因素

动物营养学报 2019年第11期31卷 4949-4958页

作者：王鑫李光鹏内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室

肉质性状指标包括pH、色泽、嫩度、系水力、肌内脂肪含量和脂肪酸组成等,这些性状受到包括牛的品种、年龄、性别和基因型等遗传因素以及饲养管理和屠宰工艺等环境因素的共同影响。同一品种牛,屠宰月龄较小的母牛或阉牛,其肉品质较好;不... 详细信息

肉质性状指标包括pH、色泽、嫩度、系水力、肌内脂肪含量和脂肪酸组成等,这些性状受到包括牛的品种、年龄、性别和基因型等遗传因素以及饲养管理和屠宰工艺等环境因素的共同影响。同一品种牛,屠宰月龄较小的母牛或阉牛,其肉品质较好;不同品种或群体的牛,会因钙蛋白酶抑制素、钙蛋白酶、二酰甘油酰基转移酶1、脂肪酸结合蛋白4及硬脂酰辅酶A去饱和酶等基因的多态性而产生肉质性状差异。屠宰前的应激反应和屠宰后的排酸方式因影响肉的极限pH而改变肉品质。肌肉生长抑制素基因突变促进产肉性能的同时,可能对肉品质有一定影响。本文就影响牛肉品质的若干因素研究进展作一综述。

关键词：牛肉色泽嫩度肌内脂肪脂肪酸合成肌肉生长抑制素

The Dynamic Mechanism of Endogenous Viral MuERV-L/MERVL in Mouse Somatic Cell Nuclear Transfer

在线全文

学校读者我要写书评

暂无评论

The Dynamic Mechanism of Endogenous Viral MuERV-L/MERVL in M...

PRMT5 enhances the generation of induced pluripotent stemcells(iPSCs) from dairy goat embryonic fibroblasts(GEF) through down regulation of P53

2017年第十五届中国北方实验动物科技年会

作者： Lei Yang lishuang Song Xuefei liu lige Bai guangpeng li The Key Laboratory of the National Education Ministry for Mammalian Reproductive Biology and Biotechnology Inner Mongolia University

Despite the success in generating animals through somatic cell nuclear transfer（SCNT） in many different species,a common problem associated with SCNT is the low *** zygotic（or embryonic）genome activation（ZGA or EGA）,a large number of endogenous retroviruses（ERVs） are expressed,including the murine endogenous retrovirus-L（MuERVL/MERVL）.Here,we generated a series of MERVLreporter mouse stains,which allows us to detect the ZGA of SCNT *** found the majority of SCNT embryos are ZGA failure,and histone H3 lysine 27 trimethylation（H3 K27 me3） as a barrier preventing SCNT *** of the H3 K27 me3 specific demethylases KDM6 A rather than KDM6 B improves the efficiency of ***,knock-down KDM6 B also facilitates ZGA and consequently improves the SCNT *** also revealed that knock-down KDM6 B increased the rate of SCNTderived Duchenne Muscular Dystrophy（DMD） embryonic stem cells establishment.

关键词： SCNT ESCs MERVL embryo KDM6A KDM6B H3K27me3

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

PRMT5 enhances the generation of induced pluripotent stemcel...

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Zhili Chu Bowen Niu Haijing Zhu Xin He Chunling Bai guangpeng li JinlianHua College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering &Technology Northwest A&F University Key Laboratory for Mammalian Reproductive Biology and Biotechnology Ministry of Education Inner Mongolia University

PRMT5, protein arginine methyltransferase 5 is thought to play a role in epigenetic reprogrammingin mouse germ cells. However, there were little information on the expressionprofile of PRMT5 and their effects on the generation of livestock induced pluripotent stemcells(iPSCs) from somatic cells. Here, we explored the PRMT5 expression profiles in dairygoat and its effects on the derivation of iPSCs from dairy goat embryonic fibroblasts(GEF).The results showed that PRMT5 is a conservative gene and were expressed widely in varioustissues and different ages’ testes. PRMT5 overexpression in combination withOCT3/4, SOX2, KLF4 and cMYC(POSKM) could significantly increased the number of APpositive iPSlike colonies derived-GEF compared with OSKM alone in dairy goat. Moreover,our results demonstrated that PRMT5 overexpression stimulated GEF proliferation, anddown-regulated P53, P21(a target genes of p53) and apoptotic markerCapase3 to enhancesthe somatic cell reprogramming. This study provides an efficient model to study the mechanisms on goat somatic cell reprogramming and differentiation in the future.

关键词： embryonic stem cells(ESCs) Induced pluripotent stem cells(iPSCs) Proteina rginine methyl transferase 5(PRMT5) Pluripotency dairy goat

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国肉牛分子与基因修饰育种研究进展

遗传 2017年第11期39卷 984-1015页

作者：佟彬张立李光鹏内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室呼和浩特010071

随着世界肉牛产业科技的快速发展,我国肉牛产业的整体水平得到明显提高并取得丰硕成果。肉牛育种技术实现了由常规杂交育种向分子标记辅助育种、全基因组选择育种和基因组修饰育种的技术跨越,揭示出大量与生长发育、肉质品质、繁殖与疾... 详细信息

随着世界肉牛产业科技的快速发展,我国肉牛产业的整体水平得到明显提高并取得丰硕成果。肉牛育种技术实现了由常规杂交育种向分子标记辅助育种、全基因组选择育种和基因组修饰育种的技术跨越,揭示出大量与生长发育、肉质品质、繁殖与疾病等相关的候选基因与分子标记,并逐步应用于肉牛育种实践。与生长发育性状相关的基因或分子标记主要集中在生长激素基因、生肌调节因子家族、肌肉生长抑制因子和胰岛素样生长因子等;参与肉质形成的基因主要集中在脂肪酸运输与沉积相关信号通路、钙蛋白酶信号通路、生肌调节因子家族与肌肉生长抑制因子等;繁殖性状相关基因或分子标记主要集中在Gn RH-FSHR-LH、生长分化因子9、催乳素受体和Fox O1等;抗病相关基因主要有MHC基因家族、TOLL样受体4基因等。目前,利用精准基因编辑技术已培育出促生长发育与提高肉品质的肉牛育种新材料。本文总结了近年来我国在肉牛分子与基因组修饰育种领域取得的研究进展,以期为我国肉牛遗传育种技术研究提供参考和借鉴。

关键词：肉牛分子育种基因组修饰育种