Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer:a review

作     者：Fengxia YIN Hui LIU Shorgan BOU Guangpeng LI 

作者机构：The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnologythe College of Life SciencesInner Mongolia UniversityHohhot 010070China 

出 版 物：《Frontiers of Agricultural Science and Engineering》 (农业科学与工程前沿（英文版）)

年 卷 期：2014年第1卷第2期

页      面：104-113页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 070202[理学-粒子物理与原子核物理] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：This work was supported by the National Basic Research Program of China(2012CB22306) the National Natural Science Foundation of China(31372289). 

主　　题：nuclear reprogramming somatic cell transcription factors transcriptomics 

摘      要：Oocytes are unique cells with the inherent capability to reprogram nuclei.The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer.The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming.The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin.The oocyte first obtains its reprogramming capability in the early fetal follicle,and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes.The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming.Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells.The maternal pronucleus exerts a predominant,transcriptiondependent effect on embryo cytofragmentation,with a lesser effect imposed by the ooplasm and the paternal pronucleus.With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.