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Migration to gliomas of bone mesenchymal stem cells transfected with cytosine deaminase gene following transplantation in vivo

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Neural Regeneration Research 2011年第19期6卷 1462-1466页

作者： guangchun Ji Fei song Qi Xing Jian Liu Kedong song Daqing Zhang Zihan Wang The Second Affiliated Clinical College of Dalian Medical University Dalian 116023 Liaoning Province China The First Affiliated Clinical College of Dalian Medical University Dalian 116011 Liaoning Province China Institute of Chemical Engineering Dalian University of Technology/Dalian Stem Cell and Tissue Engineering Research and Development Center Dalian 116024 Liaoning Province China Dalian Central Hospital Dalian 116033 Liaoning Province China

This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gone （BMSCs-CD/eGFP） after transplantation in vivo through three pathways. In addition, we examined the tropism of these cells to glioma. Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotactic inoculation method. When tumors were 7 days old, rats were inoculated with lx106 BMSCs-CD/eGFP cells via the tumor-bearing internal carotid artery, the contralateral hemisphere and the tumor-bearing glioma. Fluorescence microscopy revealed that BMSCs-CD/eGFP exhibited a strong capacity for migration to tumors. BMSCs-CD/eGFP transplanted via the tumor-bearing intemal carotid artery were observed to distribute in glioma tissues. BMSCs-CD/eGFP inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues. BMSCs-CD/eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.

关键词： cytosine deaminase gone bone mesenchymal stem cells glioma transduction migration

Genomics in Neurological Disorders

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Genomics, Proteomics & Bioinformatics 2014年第4期12卷 156-163页

作者： guangchun Han Jiya Sun Jiajia Wang Zhouxian Bai Fuhai song Hongxing Lei CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences University of Chinese Academy of Sciences Center of Alzheimer’s Disease Beijing Institute for Brain Disorders

Neurological disorders comprise a variety of complex diseases in the central nervous system, which can be roughly classified as neurodegenerative diseases and psychiatric disorders. The basic and translational research of neurological disorders has been hindered by the difficulty in accessing the pathological center （i.e., the brain） in live patients. The rapid advancement of sequencing and array technologies has made it possible to investigate the disease mechanism and biomarkers from a systems perspective. In this review, recent progresses in the discovery of novel risk genes, treatment targets and peripheral biomarkers employing genomic technologies will be dis- cussed. Our major focus will be on two of the most heavily investigated neurological disorders, namely Alzheimer＇s disease and autism spectrum disorder.

关键词： Brain transcriptome Genome-wide associationstudy Whole exome sequencing Epigenome profiling Biomarker

Towards Personalized Intervention for Alzheimer's Disease

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Genomics, Proteomics & Bioinformatics 2016年第5期14卷 289-297页

作者： Xing Peng Peiqi Xing Xiuhui Li Ying Qian Fuhai song Zhouxian Bai guangchun Han Hongxing Lei CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of Sciences Cunji Medical School University of Chinese Academy of Sciences Center of Alzheimer’s Disease Beijing Institute for Brain Disorders

Alzheimer＇s disease （AD） remains to be a grand challenge for the international community despite over a century of exploration. A key factor likely accounting for such a situation is the vast heterogeneity in the disease etiology, which involves very complex and divergent pathways. Therefore, intervention strategies shall be tailored for subgroups of AD patients. Both demographic and in-depth information is needed for patient stratification. The demographic information includes primarily APOE genotype, age, gender, education, environmental exposure, life style, and medical history, whereas in-depth information stems from genome sequencing, brain imaging, peripheral biomarkers, and even functional assays on neurons derived from patient-specific induced pluripotent cells （iPSCs）. Comprehensive information collection, better understanding of the disease mech- anisms, and diversified strategies of drug development would help with more effective intervention in the foreseeable future.

关键词： Alzheimer＇s disease Demographic information Genome Peripheral biomarkers iPSC technology

Web Resources for Stem Cell Research

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Genomics, Proteomics & Bioinformatics 2015年第1期13卷 40-45页

作者： Ting Wei Xing Peng Lili Ye Jiajia Wang Fuhai song Zhouxian Bai guangchun Han Fengmin Ji Hongxing Lei College of Life Sciences and Bioengineering Beijing Jiaotong University CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences University of Chinese Academy of Sciences Center of Alzheimer's Disease Beijing Institute for Brain Disorders

In this short review, we have presented a brief overview on major web resources relevant to stem cell research. To facilitate more efficient use of these resources, we have provided a preliminary rating based on our o... 详细信息

关键词： Reprogramming Direct conversion Physical interaction Regulatory interaction Network

Inhibitory effects of cytosine deaminase gene-transfected bone marrow mesenchymal stem cells on glioma cell proliferation

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Neural Regeneration Research 2011年第16期6卷 1238-1242页

作者： Fei song Qi Xing Kedong song Jian Liu guangchun Ji Yufang Ma Tianqing Liu Minghai Wei Xuehu Ma Dalian R&D Center for Stem Cells and Tissue Engineering Dalian University of Technology Dalian 116024 Liaoning Province China Department of Neurosurgery Second Hospital Affiliated to Dalian Medical University Dalian 116027 Liaoning Province China Department of Anesthesiology First Hospital Affiliated to Dalian Medical University Dalian 116011 Liaoning Province China Department of Biochemistry and Molecular Biology Dalian Medical University Dalian 116044 Liaoning Province China

Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase （CD） gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma. Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days. MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.

关键词： magnetic resonance imaging glioma gene therapy cytosine deaminase gene bone marrow mesenchymal stem cells lentivirus

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中国共产党伟大建党精神的形成

河北旅游职业学院学报 2022年第3期27卷 12-16页

作者：宋广春中共承德市委党校党史党建教研室河北承德067000

习总书记在2021年7.1讲话中首次阐述了建党精神。伟大建党精神的形成有着特定的历史背景和实践基础。伟大实践塑造伟大精神。伟大建党精神产生于伟大的建党实践。建党实践不是一时、一地、一人之举,而是历经多年、在中华大地上由广大爱... 详细信息

习总书记在2021年7.1讲话中首次阐述了建党精神。伟大建党精神的形成有着特定的历史背景和实践基础。伟大实践塑造伟大精神。伟大建党精神产生于伟大的建党实践。建党实践不是一时、一地、一人之举,而是历经多年、在中华大地上由广大爱国志士发起的一次伟大壮举。在充满斗争、艰险的过程中孕育出了伟大的建党精神。

关键词：伟大建党精神形成历史背景实践活动

附加超声对6061-T6铝合金/Q235钢搅拌摩擦焊搭接工艺优化

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焊接学报 2022年第5期43卷 69-75,I0007,I0008页

作者：刘涛高嵩肖光春吴程浩石磊孙志平齐鲁工业大学(山东省科学院) 济南250353 山东大学材料液固结构演变与加工教育部重点实验室济南250061

采用超声振动强化搅拌摩擦焊接工艺实现了6 061-T6铝合金和Q235钢异种金属的有效连接,考察了超声能量对焊缝成形、接头组织、力学性能以及焊接载荷的影响.结果表明,施加超声能量可以显著改善焊缝表面成形,增加铝/钢界面区和焊核区的宽度... 详细信息

采用超声振动强化搅拌摩擦焊接工艺实现了6 061-T6铝合金和Q235钢异种金属的有效连接,考察了超声能量对焊缝成形、接头组织、力学性能以及焊接载荷的影响.结果表明,施加超声能量可以显著改善焊缝表面成形,增加铝/钢界面区和焊核区的宽度.超声振动细化了焊核区和热力影响区的晶粒组织,改变了搭接接头的断裂机制和断裂位置,提高了接头的力学性能.对焊接过程中焊接载荷的变化进行分析,发现施加超声能量后搅拌头扭矩、焊接轴向力和主轴输出功率均有所下降.

关键词：搅拌摩擦焊超声能量铝/钢组织与性能焊接载荷

STRUCTURE OF LIQUID Al-Si ALLOYS

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Acta Metallurgica Sinica(English Letters) 1994年第1期7卷 49-53页

作者： GUI Manchang, JIA Jun, song guangchun, LI Peiyong, LI QingchunHarbin Institute of Technology, Harbin, China

In the light of DSC, electronic microprobe and X-ray diffration analyses of the melt-spinning ribbons and the structures of liquid hypoeutectic Al-7%Si, euteclic Al-12.7%Si and hypereutectic A1-18%Si alloys have been investigated. The results show that the Al-Si clusters in liquid hypoeutectic Al-Si alloy, the Si-Si clusters in liquid eutectic and hypereutectic Al-Si alloys exist at low temperatures, however, the size of clusters decreases gradurally with increasing temperature. This process in which inhomogeneous liquid turns into homogeneous one happens in a range of temperatures.

关键词： Al-Si alloy liquid alloy cluster

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短碳纤维增强铝基复合材料

化工学报 2005年第6期56卷 1130-1133页

作者：高嵩姚广春东北大学材料与冶金学院

通过化学镀再电镀的方法,在碳纤维表面镀上Cu镀层,制备C/Cu复合丝,并在硼酸的保护下,利用非真空条件下的液态机械搅拌法制备短碳纤维增强铝基复合材料,研究了碳纤维在复合材料中的分散程度,铜镀层存在状态及C/Al复合材料的拉伸性能.实... 详细信息

通过化学镀再电镀的方法,在碳纤维表面镀上Cu镀层,制备C/Cu复合丝,并在硼酸的保护下,利用非真空条件下的液态机械搅拌法制备短碳纤维增强铝基复合材料,研究了碳纤维在复合材料中的分散程度,铜镀层存在状态及C/Al复合材料的拉伸性能.实验结果表明:在硼酸存在下,大大降低了铜的氧化程度,碳纤维分散均匀且没有损伤,少量硼酸的加入,对复合材料的力学性能没有影响,该复合材料的抗拉强度随碳纤维含量的增加而增加,其抗拉强度较基体材料提高50%以上,但塑性指标却明显下降.

关键词：碳纤维化学镀电镀复合材料抗氧化机械性能