Migration to gliomas of bone mesenchymal stem cells transfected with cytosine deaminase gene following transplantation in vivo

作     者：Guangchun Ji Fei Song Qi Xing Jian Liu Kedong Song Daqing Zhang Zihan Wang 

作者机构：The Second Affiliated Clinical College of Dalian Medical University Dalian 116023 Liaoning Province China The First Affiliated Clinical College of Dalian Medical University Dalian 116011 Liaoning Province China Institute of Chemical Engineering Dalian University of Technology/Dalian Stem Cell and Tissue Engineering Research and Development Center Dalian 116024 Liaoning Province China Dalian Central Hospital Dalian 116033 Liaoning Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第19期

页      面：1462-1466页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the Natural Science Foundation of Liaoning Province  China  No. 20092165 

主　　题：cytosine deaminase gone bone mesenchymal stem cells glioma transduction migration 

摘      要：This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gone （BMSCs-CD/eGFP） after transplantation in vivo through three pathways. In addition, we examined the tropism of these cells to glioma. Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotactic inoculation method. When tumors were 7 days old, rats were inoculated with lx106 BMSCs-CD/eGFP cells via the tumor-bearing internal carotid artery, the contralateral hemisphere and the tumor-bearing glioma. Fluorescence microscopy revealed that BMSCs-CD/eGFP exhibited a strong capacity for migration to tumors. BMSCs-CD/eGFP transplanted via the tumor-bearing intemal carotid artery were observed to distribute in glioma tissues. BMSCs-CD/eGFP inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues. BMSCs-CD/eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.