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Effect of Guizhi Fuling capsule and its extracts on human breast cancer cells proliferation

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中国药理学与毒理学杂志 2017年第10期31卷 1013-1014页

作者： Zi-ru YU Li LI Jin-hua WANG Zhen-zhong WANG Wei XIAO guan-hua DU Beijing Key Laboratory of Drug Targets Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Jiangsu Kanion Pharmaceutical CoLtd

OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient(API)and its fractions on human breast cancer cells proliferation by high-throughput screening *** The crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule,and 929 standard fractions were obtained by the optimal separation *** B(SRB)method was used to evaluate the effects of the Guizhi Fuling capsule API and929 kinds of fractions on the proliferation of human breast cancer cells MCF-7 and *** The Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells' proliferate at low concentration following 72 h treatment;some samples of 929 fractions(5μg·mL^(-1))was found to have a breast cancer cell growth inhibition rate above 50%,without toxicity on HUVECs *** The API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells,with significant concentration-and time-dependent manner.

关键词： high throughput screening Guizhi Fuling capsule breast cancer MCF-7 cell MDA-MB-231 cell sulforhodamine B

18β-Glycyrrhetinic Acid Improves Cardiac Diastolic Function by Attenuating Intracellular Calcium Overload

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Current Medical Science 2020年第4期40卷 654-661页

作者： Jun HAN guan-hua SU Yu-hui WANG Yong-xin LU Hong-liang ZHAO Xin-xin ShuaI Department of Cardiology Wuhan Fourth HospitalPuai HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430033China Department of Cardiology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Summary:Ranolazine,a late sodium current inhibitor,has been demonstrated to be effective on heart failure.18B-glycyrrhetinic acid(18β-GA)has the similar inhibitory effect on late sodium ***,its effect on diastolic function is still *** study aimed to determine whether 18β-GA can improve the diastolic function and to explore the underlying *** male Sprague Dawley(SD)rats of Langendorff model were randomly divided into the following groups:group A,normal cardiac perfusion group;group B,ischemia-reperfusion group;group C,ischemia-reperfusion with anemoniasulcata toxinⅡ(ATX-Ⅱ);group D,ranolazine group;and group E,18β-GA group with four different ***,a pressure-overloaded rat model induced by trans-aortic constriction(TAC)was *** and hemodynamics were used to evaluate diastolic function at 14th day after *** of free intracellular calcium(Ca27)concentration was indirectly detected by laser scanning confocal microscope to confirm the inhibition of late sodium *** the intervention of ATX-Ⅱon ischemia reperfusion injury group,5 umol/L ranolazine,and 5,10,20,40μmol/L 18β-GA could improve ATX-I-induced cardiac diastolic dysfunction.630 mg/kg glycyrrhizin tablets could improve cardiac diastolic function in the pressure-overloaded rats.18B-GA and ranolazine had similar effects on reducing the free calcium in *** study demonstrates that 18B-GA and glycyrrhizin could improve diastolic dysfunction induced by ischemia-reperfusion injury in Langendorff-perfused rat hearts and pressure-overloaded *** mechanism may be attributed to the inhibition of enhanced late sodium currents.

关键词： glycyrrhetinic acid diastolic function calcium overload

Influence of pump intensity on atomic spin relaxation in a vapor cell

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Chinese Physics B 2019年第11期28卷 339-343页

作者： Chen Yang guan-hua Zuo Zhuang-Zhuang Tian Yu-Chi Zhang Tian-Cai Zhang State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China College of Physics and Electronics Engineering Shanxi UniversityTaiyuan 030006China

Atomic spin relaxation in a vapor cell, which can be characterized by the magnetic resonance linewidth(MRL),is an important parameter that eventually determines the sensitivity of an atomic magnetometer. In this paper, we have extensively studied how the pump intensity affects the spin relaxation. The experiment is performed with a cesium vapor cell, and the influence of the pump intensity on MRL is measured at room temperature at zero-field resonance. A simple model with five atomic levels of a Λ-like configuration is discussed theoretically, which can be used to represent the experimental process approximately, and the experimental results can be explained to some extent. Both the experimental and the theoretical results show a nonlinear broadening of the MRL when the pump intensity is increasing. The work helps to understand the mechanism of pump induced atomic spin relaxation in the atomic magnetometers.

关键词： atomic magnetometer atomic spin relaxation optical pumping

Identification of multi-target anti-cancer agents from TCM formula by in silico prediction and in vitro validation

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Chinese Journal of Natural Medicines 2022年第5期20卷 332-351页

作者： ZHANG Bao-Yue ZHENG Yi-Fu ZHAO Jun KANG De WANG Zhe XU Lv-Jie LIU Ai-Lin DU guan-hua State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China School of Life Sciences Tsinghua UniversityBeijing 100084China

Cancer is a complex disease associated with multiple gene mutations and malignant phenotypes,and multi-target drugs provide a promising therapy idea for the treatment of *** products with abundant chemical structure types and rich pharmacological characteristics could be ideal sources for screening multi-target antineoplastic *** this paper,50 tumor-related targets were collected by searching the Therapeutic Target Database and Thomson Reuters Integrity database,and a multi-target anti-cancer prediction system based on mt-QSAR models was constructed by using naïve Bayesian and recursive partitioning algorithm for the first *** the multi-target anti-cancer prediction system,some dominant fragments that act on multiple tumor-related targets were analyzed,which could be helpful in designing multi-target anti-cancer ***-cancer traditional Chinese medicine(TCM)and its natural products were collected to form a TCM formula-based natural products library,and the potential targets of the natural products in the library were predicted by multi-target anti-cancer prediction *** a result,alkaloids,flavonoids and terpenoids were predicted to act on multiple tumor-related *** predicted targets of some representative compounds were verified according to literature review and most of the selected natural compounds were found to exert certain anti-cancer activity in vitro biological *** conclusion,the multi-target anti-cancer prediction system is very effective and reliable,and it could be further used for elucidating the functional mechanism of anti-cancer TCM formula and screening for multi-target anti-cancer *** anti-cancer natural compounds found in this paper will lay important information for further study.

关键词： Cancer Multi-target mt-QSAR model Nave Bayesian Recursive partitioning TCM formulae

Progress on the^40Ca^+ion optical clock

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Chinese Physics B 2020年第7期29卷 69-76页

作者： Baolin Zhang x Yao huang huaqing Zhang Yanmei Hao Mengyan Zeng hua guan Kelin Gao State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences(CAS)Wuhan 430071China Key Laboratory of Atomic Frequency Standards Wuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Center for Cold Atom Physics Chinese Academy of SciencesWuhan 430071China huazhong University of Science and Technology Wuhan 430074China

Progress of the ^40Ca^+ion optical clock based on the 4^2S1/2-3d ^2D5/2 electric quadrupole transition is *** setting the drive frequency to the“magic”frequencyΩ0,the frequency uncertainty caused by the scalar Stark shift and second-order Doppler shift induced by micromotion is reduced to the 10^-19 *** precisely measuring the differential static scalar polarizability∆α0,the uncertainty due to the blackbody radiation(BBR)shift(coefficient)is reduced to the 10^-19 *** the help of a second-order integrating servo algorithm,the uncertainty due to the servo error is reduced to the 10^-18 *** total fractional uncertainty of the ^40Ca^+ion optical clock is then improved to 2.2×10^-17,whereas this value is mainly restricted by the uncertainty of the BBR shift due to temperature *** state preparation is introduced together with improvements in the pulse sequence,and furthermore,a better signal to noise ratio(SNR)and less dead time are *** clock stability of a single clock is improved to 4.8×10^-15√τ(in seconds).

关键词： ^40Ca^+ion optical clocks “magic”drive frequency frequency uncertainty frequency stability

Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei,China,2013–2016

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Virologica Sinica 2022年第4期37卷 503-512页

作者： Ting Zhang Jing Li Yong-Zhong Jiang Jun-Qiang Xu Xu-hua guan Li-Qiang Wang Jie Chen Yi Liang Hubei Key Laboratory of Cell Homeostasis College of Life SciencesWuhan UniversityWuhan430072China Hubei Provincial Center for Disease Control and Prevention Wuhan430079China

Group A human rotaviruses(RVAs)annually cause the deaths of 215,000 infants and young *** understand the epidemiological characteristics and genetic evolution of RVAs,we performed sentinel surveillance on RVA prevalence in a rotavirus-surveillance network in Hubei,*** 2013 to 2016,a total of 2007 fecal samples from hospital outpatients with acute gastroenteritis were collected from four cities of Hubei *** the 2007 samples,153(7.62%)were identified positive for RVA by real-time *** infection in Hubei mainly occurred in autumn and *** highest detection rate of RVA infection was in 1–2 years old of outpatients(16.97%).No significant difference of RVA positive rate was observed between females and *** performed a phylogenetic analysis of the G/P genotypes based on the partial VP7/VP4 gene sequences of RVAs.G9P[8]was the most predominant strain in all four years but the prevalence of G2P[4]genotype increased rapidly since *** reconstructed the evolutionary time scale of RVAs in Hubei,and found that the evolutionary rates of the G9,G2,P[8],and P[4]genotypes of RVA were 1.069×10^(-3),1.029×10^(-3),1.283×10^(-3)and 1.172×10^(-3)nucleotide substitutions/site/year,***,using a molecular clock model,we showed that most G9,G2,P[8],and P[4]genotype strains dated from the recent ancestor in 2005,2005,1993,and 2013,*** finding of the distribution of RVAs in infants and young children in Hubei Province will contribute to the understanding of the epidemiological characteristics and genetic evolution of RVAs in China.

关键词： Group A human rotavirus(RVA) Prevalence Phylogenetic analysis Evolution Hubei Province

2-DG regain sensitivity of drug resistant colon cancer on 5-FU via blocking LKB1/Nrf2/Stat3 mediated metabolic reprogramming

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中国药理学与毒理学杂志 2019年第10期 887-888页

作者： WANG Lin YU Zi-ru WANG Rui DU guan-hua Beijing Key Laboratory of Drug Targets Research and New Drug Screening Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College guangdong Pharmaceutical University

OBJECTIVE Unusual plasticity on the metabolic reprogramming is linked to therapeutic resistance to5-FU on cancer cells. As a glycolysis inhibitor, 2-DG monotherapy is not ideal on colon cancer therapy in *** this study, we supposed the combination of 2-DG and 5-FU might be a useful therapeutic strategy for resistant colon cancer. METHODS Cel viability was measured using CCK8 assay to determine the synergism of 2-DG on 5-FU in resistant colon cancer in vitro. Mitochondrial stress was measured according to the instruction provided by Seahorse Bioscience. Nude mice bearing 5-FU sensitive or resistant colon cancer were used for in vivo study to determine the synergism of 2-DG to 5-FU on resistant colon cancer. Reactive oxygen species(ROS) in tissue homogenate and the content of triglyceride/acetoacetic acid in serumwere determined by commercial kits. The expression of HIF1α, LKB1, HKII, Nrf2, Stat3, FASN and SCD1 were detected by immunohistochemistry or Western blotting. RESULTS 2-DG efficiently regained the sensitivity of 5-FU in resistant colon cancer both in vitro and in vivo.2-DG significantly increased ROS, lipid drops(LDs) and triglyceride(TG) while decreased acetoacetic acid(Acac)in serum. CONCLUSION Combination of 2-DG and 5-FU is a viable therapeutic approach for the treatment of resistant colon cancer. The synergism of 2-DG was achieved by reversing glucolipid metabolic reprogramming through regulating HIF1/Nrf2/Stat3 mediated signal pathway.

关键词： 5-FU resistant colon cancer 2-DG mitochondria HIF1 Nrf2 Stat3

Xiao-Xu-Ming decoction protects against chronic cerebral ischemia injury in rats and profile of differentially expression proteins analysis in hippocampus

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中国药理学与毒理学杂志 2018年第4期32卷 310-311页

作者： Yue-hua WANG Yin-lin YANG Xiao CHENG Jun ZHANG Wan LI Wei-han LI guan-hua DU Beijing Key Laboratory of Drug Target Identification and New Drug Screening Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China

OBJECTIVE Vascular dementia(VD) refers to a progressive decline in memory and cognitive function caused by chronic cerebral ischemia.2-Vessels occlusion(2-VO) has been widely used as a model of ***-Xu-Ming decoction,a well-known traditional Chinese medicine ***,has been widely used to treat stroke and sequelae of *** present study was to investigate the mechanism of Xiao-Xu-Ming decoction(XXM) against chronic cerebral ischemia injury in *** After XXM treatment,rats were performed a memory testing with Morris water maze and motor ability testing using prehensile test and inclined screen *** plasticity was observed by immunofluorescent staining with MAP2 *** expressed proteins of rat hippocampus were analyzed by Label-free quantitative *** XXM significantly alleviated 2-VOinduced learning and memory deficits,motor ability dysfunction,and neuronal plasticity injury in *** mechanism might be involved in up-regulation of 39 proteins and down-regulation of 13 proteins in the hippocampus of rats after XXM treatment vs 2-VO group *** ontology and pathway analysis showed that the regulated proteins are mainly involved in oxidation reduction process,intracellular signaling cascade process,and protein catabolic process,*** signal pathways are mainly involved in ubiquitin mediated proteolysis and phosphatidylinositol signaling *** Current findings provide new insights into the molecular mechanisms of XXM on chronic cerebral ischemia.

关键词：血管性痴呆慢性脑缺血治疗方法临床分析

Lipoprotein(a) is associated with coronary atheroma progression: analysis from a serial coronary computed tomography angiography study

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Journal of Geriatric Cardiology 2021年第12期18卷 996-1007页

作者： Xi WANG Dong-Kai SHAN guan-hua DOU Yi-Pu DING Jing JING He-Bin CHE Jun-Jie YANG Yun-Dai CHEN Medical School of Chinese PLA BeijingChina Department of Cardiology the Sixth Medical CentreChinese PLA General HospitalBeijingChina Department of Cardiology the Second Medical CentreChinese PLA General HospitalBeijingChina School of Medicine Nankai UniversityTianjinChina Medical Big Data Research Centre Chinese PLA General HospitalBeijingChina

BACKGROUND Lipoprotein(a)[Lp(a)]has been closely related to coronary atherosclerosis and might affect perivascular in-flammation due to its proinflammatory ***,there are limited data about Lp(a)and related perivascular inflam-mation on coronary atheroma ***,this study aimed to investigate the associations between Lp(a)and the peri-vascular fat attenuation index(FAI)with coronary atheroma progression detected by coronary computed tomography angio-graphy(CCTA).METHODS Patients who underwent serial CCTA examinations without a history of revascularization and with available data for Lp(a)within one month before or after baseline and follow-up CCTA imaging scans were considered to be *** quantitative analyses were performed to obtain the total plaque volume(TPV)and the perivascular *** plaque pro-gression(PP)was defined as a≥10%increase in the change of the TPV at the patient level or the presence of new-onset coronary atheroma *** associations between Lp(a)or the perivascular FAI with PP were examined by multivariate logistic *** A total of 116 patients were ultimately enrolled in the present study with a mean CCTA interscan interval of 30.80±13.50 *** the 116 patients(mean age:53.49±10.21 years,males:83.6%),32 patients presented PP during the follow-up ***(a)levels were significantly higher among PP patients than those among non-PP patients at both baseline[15.80(9.09−33.60)mg/dL vs.10.50(4.75−19.71)mg/dL,P=0.029]and follow-up[20.60(10.45−34.55)mg/dL vs.8.77(5.00−18.78)mg/dL,P=0.004].However,there were no differences in the perivascular FAI between PP group and non-PP group at either baseline or *** logistic regression analysis showed that elevated baseline Lp(a)level(OR=1.031,95%CI:1.005−1.058,P=0.019)was an independent risk factor for PP after adjustment for other conventional *** Lp(a)was independently associated with coronary atheroma pr

关键词： Lp(a) coronary elevated