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Gene Cloning and Expression Analysis of G Protein αq Subunit from Helicoverpa assulta (Guenée)

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Agricultural Sciences in China 2008年第2期7卷 187-192页

作者： QIAO Qi LI Hai-chao YUAN guo-hui guo xian-ru LUO Mei-hao College of Plant Protection Henan Agricultural University Zhengzhou 450002 P.R.China

The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta （Guen6e） by reverse transcription polymerase chain reaction （RT-PCR） and named as HassGαq. Sequencing analysis showed that the fulllength of HassGαq open reading frame （ORF） is 1 062 bp, 353 amino acid residues are encoded. The predicted molecular weights （MW） and isoelectric point （PI） are 41.5 kD and 5.15, respectively. HassGαq gene was then constructed into expression vector pGEX-4T-2 for over expression in prokaryotic cells. The SDS-PAGE and Western blot analysis showed that induced by Isopropyl-β-D-Thiogalactoside （IPTG）, the GST-HassGαq fusion protein is expressed in Escherichia coil BL21, and its MW was found to be about 66 kD nearly equal to the predicted. In addition, RT-PCR analysis showed that the expressions of HassGαq are not tissue specific.

关键词： Helicoverpa assulta G protein α subunit gene cloning prokaryotic expression expression pattern

Identification of summer nectar plants contributing to outbreaks of Mythimna separata(Walker)(Lepidoptera: Noctuidae) in North China

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Journal of Integrative Agriculture 2018年第7期17卷 1516-1526页

作者： guo Pei WANG Gao-ping JIN Li-jie FAN Xing-qi HE Han-lin ZHOU Pei-wen guo xian-ru LI Wei-zheng YUAN guo-hui College of Plant Protection Henan Agricultural University

The presence of adequate nectar sources along the migratory pathway is a prerequisite for moths of oriental armyworm, Mythimna separata, to reach target habitats and lay eggs. Identification of plant species and assessment of their suitability as wild nectar sources along the summer migration pathway of adult M. separata are critical steps in making effective forecasts of second-and third-generation armyworm outbreaks in North China. We explored five aspects of moth-nectar source relationships：（1） Field investigations in 2014 and 2015 showed that the florescence of chaste tree, Vitex negundo var. heterophylla, a primary nectar plant in apiculture, coincided with the summer migration of oriental armyworm moths. The flowers of chaste tree opened day and night and M. separata moths were found actively flying around and resting on tree.（2） Identification of moth-borne pollen by scanning electronic microscopy（SEM） micrographs in 2015 and 2016 revealed that 15.4% of 369 moths trapped by blacklight traps, 17.1% of 557 moths captured by vertical-pointing searchlight traps and 24.4% of 311 moths caught by pheromone traps were found with pollen attached to their proboscis. A total of 228 pollen-carrying individuals out of 1237 armyworm moths were captured by the three types of traps. The three highest percentages of pollen-bearing armyworm moths were carrying pollen of Toona sp.（59.65%）, Ligustrum sp.（27.19%） and Syringa sp.（4.39%）. In total, 14 types of pollen were detected where three were identified to species, 10 to genus and one as an unknown species.（3） Analysis of geographical distribution, flowering periods and relative abundances of candidate nectar plants suggested that Chinese toon, Toona sinensis, and privet, Ligustrum lucidum, were two important nectar plant species.（4） Analysis on relationship between pollen and ovarian development showed that 14, 15 and 10 females carrying pollen of T. sinensis had ovaries at developmental stages Ⅰ, Ⅱ and Ⅲ, respectively. Many

关键词： Mythimna separata pollen ovarian development nectar plant

Population genetic variation and historical dynamics of the natural enemy insect Propylea japonica(Coleoptera: Coccinellidae) in China

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Journal of Integrative Agriculture 2023年第8期22卷 2456-2469页

作者： WANG Meng-qi ZHAGN Hong-rui XI Yu-qiang WANG Gao-ping ZHAO Man ZHANG Li-juan guo xian-ru Department of Entomology College of Plant ProtectionHenan Agricultural UniversityZhengzhou 450002P.R.China College of Plant Protection Yunnan Agricultural UniversityKunming 650201P.R.China Suifenhe Bureau of Agriculture and rural Affairs Suifenhe 157300P.R.China Henan International Laboratory for Green Pest Control/College of Plant Protection Henan Agricultural UniversityZhengzhou 450002P.R.China

Propylea japonica(Coleoptera:Coccinellidae)is a natural enemy insect with a wide range of predation in Chinese mainland and is commonly used in pest ***,its genetic pattern(i.e.,genetic variation,genetic structure,and historical population dynamics)is still unclear,impeding the development of biological control of insect *** genetic research has the potential to optimize strategies at different stages of the biological control *** study used 23 nuclear microsatellite sites and mitochondrial COI genes to investigate the population genetics of Propylea japonica based on 462 specimens collected from 30 sampling sites in *** microsatellite dataset showed a moderate level of genetic diversity,but the mitochondrial genes showed a high level of genetic *** from the Yellow River basin were more genetically diverse than those in the Yangtze River *** japonica has not yet formed a significant genealogical structure in China,but there was a population structure signal to some extent,which may be caused by frequent gene flow between *** species has experienced population expansion after a bottleneck,potentially thanks to the tri-trophic plant–insect–natural enemy *** of population genetics is of importance in using predators to control *** study complements existing knowledge of an important natural predator in agroecosystems through estimating its genetic diversity and population differentiation and speculating about historical dynamics.

关键词： demographic history genetic differentiation genetic diversity population expansion microsatellite mitochondrial Col

Acquired sensorineural hearing loss,oxidative stress,and microRNAs

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Neural Regeneration Research 2025年第9期20卷 2513-2519页

作者： Desmond A.Nunez ru C.guo Division of Otolaryngology–Head&Neck Surgery Department of SurgeryFaculty of MedicineThe University of British ColumbiaVancouverBCCanada Division of Otolaryngology–Head&Neck Surgery Gordon&Leslie Diamond Health Care CentreVancouver General HospitalVancouverBCCanada Faculty of Medicine–The University of British Columbia VancouverBCCanada

Hearing loss is the third leading cause of human ***-related hearing loss,one type of acquired sensorineural hearing loss,is largely responsible for this escalating global health ***-induced,ototoxic,and idiopathic sudden sensorineural are other less common types of acquired hearing *** etiology of these conditions is complex and multi-fa ctorial involving an interplay of genetic and environmental *** stress has recently been proposed as a likely linking cause in most types of acquired sensorineural hearing *** non-coding RNA sequences known as microRNAs(miRNAs)have increasingly been shown to play a role in cellular hypoxia and oxidative stress responses including promoting an apoptotic *** hair cell death is a central histopathological finding in sensorineural hearing *** these cells do not regenerate in humans,it underlies the irreversibility of human age-related hearing *** EMBASE,Ovid MEDLINE,Web of Science Core Collection,and *** databases over the period August 1,2018 to July 31,2023 were searched with"hearing loss,""hypoxamiRs,""hypoxia,""microRNAs,""ischemia,"and"oxidative stress"text words for English language primary study publications or registered clinical *** red clinical trials known to the senior author we re also assessed.A total of 222studies were thus *** excluding duplicates,editorials,retra ctions,secondary research studies,and non-English language articles,39 primary studies and clinical trials underwent full-text *** resulted in 11 animal,in vitro,and/or human subject journal articles and 8 registered clinical trial database entries which form the basis of this narrative *** miR-34a and miR-29b levels increase with age in *** miRNAs were demonstrated in human neuroblastoma and murine cochlear cell lines to target Sirtuin 1/peroxisome proliferato r-activated receptor gamma coactivator-1-alpha(SIRT1/P GC-1α),SIRT

关键词： hearing loss hypoxia microRNAs oxidative stress sensorineural

烟实夜蛾触角普通气味结合蛋白ⅡcDNA的克隆、序列分析及在大肠杆菌中的表达

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昆虫学报 2005年第1期48卷 18-23页

作者：巩中军原国辉郭线茹安世恒河南农业大学植物保护学院郑州450002 浙江大学应用昆虫学研究所杭州310029

利用RT PCR技术扩增了编码烟实夜蛾Helicoverpaassulta雌、雄虫触角普通气味结合蛋白Ⅱ的cDNA片段 ,将其克隆至pGEM TEasy载体 ,获得了普通气味结合蛋白Ⅱ基因成熟蛋白阅读框序列。将该基因重组到表达型质粒pET 30a(+)中 ,并转化入原核... 详细信息

利用RT PCR技术扩增了编码烟实夜蛾Helicoverpaassulta雌、雄虫触角普通气味结合蛋白Ⅱ的cDNA片段 ,将其克隆至pGEM TEasy载体 ,获得了普通气味结合蛋白Ⅱ基因成熟蛋白阅读框序列。将该基因重组到表达型质粒pET 30a(+)中 ,并转化入原核细胞中表达。序列测定结果表明 ,烟实夜蛾触角普通气味结合蛋白基因的成熟蛋白阅读框全长 4 89bp ,编码 16 2个氨基酸残基 ,预测分子量和等电点分别为 18 2kD和 5 35。推导的氨基酸序列与已报道的 10种昆虫普通气味结合蛋白Ⅱ高度同源 (73%～ 98% ) ,并具有气味结合蛋白的典型特征。SDS PAGE和Western印迹分析表明 ,经IPTG诱导 ,普通气味结合蛋白Ⅱ基因能在大肠杆菌BL2 1(DE3)中表达 ,电泳检测到一条约 2 3kD大小的外源蛋白 ,与预测的融合蛋白分子量大小相应。

关键词：烟实夜蛾普通气味结合蛋白触角基因克隆原核表达

不同寄主对中黑盲蝽(Adelphocoris suturalis)生长发育和繁殖的影响

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生态学报 2008年第4期28卷 1514-1520页

作者：郭小奇付晓伟封洪强邱峰郭线茹河南省农业科学院植物保护研究所郑州450002 河南农业大学植物保护学院郑州450002

实验在室内温度为(26±1)℃、相对湿度为75%±5%、自然光照的条件下,以四季豆、大豆、饭豇豆、苜蓿、转基因抗虫棉99B和非转基因棉石远321为食料,用生命表方法系统研究了不同寄主植物对中黑盲蝽实验种群生长发育、存活及繁殖的影响。结... 详细信息

实验在室内温度为(26±1)℃、相对湿度为75%±5%、自然光照的条件下,以四季豆、大豆、饭豇豆、苜蓿、转基因抗虫棉99B和非转基因棉石远321为食料,用生命表方法系统研究了不同寄主植物对中黑盲蝽实验种群生长发育、存活及繁殖的影响。结果表明中黑盲蝽(Adelphocoris suturalis)取食6种不同的寄主植物时,各虫态的发育历期、存活率、成虫寿命、产卵前期、产卵期、产卵量、5龄若虫体重等均有显著差异。用生命表参数综合评价表明,四季豆最适合中黑盲蝽的生长发育及繁殖,其次分别为苜蓿、转基因抗虫棉99B、非转基因棉石远321、饭豇豆、大豆,同时也表明转基因抗虫棉对中黑盲蝽无显著抗性。田间取食选择性试验表明,中黑盲蝽喜食饭豇豆和豇豆,可在棉田周围种植饭豇豆、豇豆作为诱集作物。

关键词：中黑盲蝽(Adelphocoris suturalis) 寄主植物生命表内禀增长率种群参数诱集作物

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烟青虫气味结合蛋白基因的克隆与序列分析

中国农业科学 2005年第9期38卷 1817-1824页

作者：吴少英王桂荣吴孔明郭予元原国辉郭线茹中国农业科学院植物保护研究所北京100094 河南农业大学植物保护学院河南农业大学植物保护学院郑州450002

根据已经报道的棉铃虫普通气味结合蛋白I(GOBP1)和性信息素结合蛋白(PBP)基因的cDNA序列,设计了2对引物,以烟青虫Helicoverpaassulta触角cDNA为模板,分别进行PCR扩增,获得2条特异性条带,约400bp。将以上两条片段分别连接到T-easy载体上... 详细信息

根据已经报道的棉铃虫普通气味结合蛋白I(GOBP1)和性信息素结合蛋白(PBP)基因的cDNA序列,设计了2对引物,以烟青虫Helicoverpaassulta触角cDNA为模板,分别进行PCR扩增,获得2条特异性条带,约400bp。将以上两条片段分别连接到T-easy载体上,获得重组子T-GOBP1-Hass和T-PBP-Hass。序列测定和结构分析表明,Hass-GOBP1开放阅读框全长441bp,编码147个氨基酸残基,预测分子量和等电点分别为17.2kD和4.71。Hass-PBP开放阅读框全长405bp,编码135个氨基酸残基,预测分子量和等电点分别为15.1kD和5.2。Hass-GOBP1和Hass-PBP具有昆虫气味结合蛋白的典型特征,即氨基酸序列中具有6个保守的半胱氨酸残基,呈酸性。这2个基因已在GenBank中登记,序列号分别是AY864774和AY864775。

关键词：烟青虫普通气味结合蛋白性信息素结合蛋白基因克隆序列分析

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烟夜蛾滞育激素基因在粟酒裂殖酵母中的表达

河南农业大学学报 2008年第4期42卷 423-426页

作者：赵特罗梅浩郭线茹原国辉河南农业大学植物保护学院河南郑州450002

利用设计的特异性引物通过RT-PCR的方法获得了烟夜蛾滞育激素(Diapause hormone,DH)基因,构建了真核表达载体pESP-2-DH,并在粟酒裂殖酵母SP-Q01中进行了诱导表达,对表达产物进行了SDS-PAGE电泳,获得了与谷胱甘肽S-转移酶(Glutathione S-... 详细信息

利用设计的特异性引物通过RT-PCR的方法获得了烟夜蛾滞育激素(Diapause hormone,DH)基因,构建了真核表达载体pESP-2-DH,并在粟酒裂殖酵母SP-Q01中进行了诱导表达,对表达产物进行了SDS-PAGE电泳,获得了与谷胱甘肽S-转移酶(Glutathione S-tranferases,GST)融合表达的蛋白,分子量约48 kDa,与预测的融合蛋白分子量大小相当。对表达的融合蛋白进行Western blotting检测,结果表明,与GST抗体产生了很强的交叉反应,表明融合蛋白得到了表达.

关键词：烟夜蛾滞育激素 RT-PCR 真核表达融合蛋白

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麦黑斑潜叶蝇幼虫和蛹在麦田的空间分布特性

华北农学报 2005年第1期20卷 86-88页

作者：郭线茹张建设孙淑君王威河南农业大学植物保护学院河南郑州450002

麦黑斑潜叶蝇近年在麦田有危害加重的趋势,为进一步了解其生态习性,确定正确的田间抽样调查方法, 作者于麦黑斑潜叶蝇为害盛期调查了其幼虫和蛹在8个品种麦田的种群密度,并采用聚集度指标法、Taylor幂法则和 m*-m回归法测定了幼虫和蛹... 详细信息

麦黑斑潜叶蝇近年在麦田有危害加重的趋势,为进一步了解其生态习性,确定正确的田间抽样调查方法, 作者于麦黑斑潜叶蝇为害盛期调查了其幼虫和蛹在8个品种麦田的种群密度,并采用聚集度指标法、Taylor幂法则和 m*-m回归法测定了幼虫和蛹在麦田的空间分布型。结果表明,幼虫和蛹在徐州26号的麦田种群密度最大,济南17 号的麦田种群密度最小;5月初麦黑斑潜叶蝇幼虫和蛹在麦田呈均匀分布。

关键词：麦田麦黑斑潜叶蝇幼虫种群密度济南17号徐州26号空间分布型聚集度指标生态习性均匀分布