Gene Cloning and Expression Analysis of G Protein αq Subunit from Helicoverpa assulta (Guenée)
Gene Cloning and Expression Analysis of G Protein αq Subunit from Helicoverpa assulta (Guenée)作者机构：College of Plant Protection Henan Agricultural University Zhengzhou 450002 P.R.China
出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)
年 卷 期：2008年第7卷第2期
页 面：187-192页
学科分类：09[农学] 0903[农学-农业资源与环境]
基 金：2007-CX-014 Excellent Youth Foundation of Heilongjiang Province of China: 074100510013
主 题：Helicoverpa assulta G protein α subunit gene cloning prokaryotic expression expression pattern
摘 要：The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta （Guen6e） by reverse transcription polymerase chain reaction （RT-PCR） and named as HassGαq. Sequencing analysis showed that the fulllength of HassGαq open reading frame （ORF） is 1 062 bp, 353 amino acid residues are encoded. The predicted molecular weights （MW） and isoelectric point （PI） are 41.5 kD and 5.15, respectively. HassGαq gene was then constructed into expression vector pGEX-4T-2 for over expression in prokaryotic cells. The SDS-PAGE and Western blot analysis showed that induced by Isopropyl-β-D-Thiogalactoside （IPTG）, the GST-HassGαq fusion protein is expressed in Escherichia coil BL21, and its MW was found to be about 66 kD nearly equal to the predicted. In addition, RT-PCR analysis showed that the expressions of HassGαq are not tissue specific.
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