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分析单克隆抗体电荷异质性的成像毛细管等电聚焦电泳技术平台方法的建立

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国际生物制品学杂志 2018年第5期41卷 213-219页

作者：杨波邢振荣武明扬程一琳韩莉杨巧娣张银川 guo amy 信达生物制药(苏州)有限公司产品开发部 215123

目的采用成像毛细管等电聚焦电泳技术（imaged capillary isoelectric focusing,iCIEF）建立分析单克隆抗体（单抗）电荷异质性的平台方法,并用不同亚型单抗（IgG1、IgG2、IgG4）确认该平台方法的适用性.方法优化该平台方法的部分参数... 详细信息

目的采用成像毛细管等电聚焦电泳技术（imaged capillary isoelectric focusing,iCIEF）建立分析单克隆抗体（单抗）电荷异质性的平台方法,并用不同亚型单抗（IgG1、IgG2、IgG4）确认该平台方法的适用性.方法优化该平台方法的部分参数,包括两性电解质和阴极稳定剂体积、聚焦时间和尿素浓度.采用3种亚型单抗（IgG1、IgG2、IgG4）对该平台方法的专属性、精密度、线性、准确度和耐用性进行验证.结果对样品（单抗）的处理条件为：3 mol/L尿素-0.5％甲基纤维素溶液70μl、两性电解质（pH3～10）4 μl、阴极稳定剂（500 mmol/L精氨酸）2 μl、等电点6.14和9.99 Marker各2μl,最终完成0.2 mg/ml单抗（样品）的制备.检测参数：预聚焦1 500V、1 min,聚焦3 000V、8 min.该平台方法的专属性良好,制剂缓冲液对检测无干扰.重复检测6份平行样品以及不同分析员于不同时间检测12份样品各成分含量的相对标准偏差均符合规定的要求.单抗（样品）终浓度为0.1～0.3 mg/ml时,主要和酸性成分的线性决定系数（R2）≥0.99,碱性成分的线性R2≥0.98.该平台方法检测样品各成分的准确度为92％～105％.耐用性实验设计结果表明,两性电解质（pH3～10）体积和毛细管批次对该平台方法有显著影响.结论建立的iCIEF平台方法分离度较高,精密度、准确度和耐用性良好,为单抗制品的电荷异质性表征和质量控制提供了更有效的工具.

关键词：电泳,毛细管等电聚焦抗体,单克隆实验室技术和方法电荷异质性

Twisted fiber microfluidics:a cutting-edge approach to 3D spiral devices

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Microsystems & Nanoengineering 2024年第1期10卷 193-202页

作者： Shunsuke Kato Daniel W.Carlson amy Q.Shen Yuanyuan guo Department of Electrical Information and Physics EngineeringSchool of EngineeringTohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Micro/Bio/Nanofluidics Unit Okinawa Institute of Science and TechnologyOnnaKunigami-gun 904-0495 OkinawaJapan Frontier Research Institute for Interdisciplinary Sciences(FRIS) Tohoku UniversityAoba-kuSendai 980-0845 MiyagiJapan Graduate School of Biomedical Engineering Tohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Department of Physiology Graduate School of MedicineTohoku UniversityAoba-kuSendai 980-8575 MiyagiJapan

The development of 3D spiral microfluidics has opened new avenues for leveraging inertial focusing to analyze small fluid volumes,thereby advancing research across chemical,physical,and biological *** traditional straight microchannels rely solely on inertial lift forces,the novel spiral geometry generates Dean drag forces,eliminating the necessity for external fields in fluid ***,fabricating 3D spiral microfluidics remains a labor-intensive and costly endeavor,hindering its widespread ***,conventional lithographic methods primarily yield 2D planar devices,thereby limiting the selection of materials and geometrical *** address these challenges,this work introduces a streamlined fabrication method for 3D spiral microfluidic devices,employing rotational force within a miniaturized thermal drawing process,termed as *** innovation allows for rapid prototyping of twisted fiber-based microfluidics featuring versatility in material selection and heightened geometric *** validate the performance of these devices,we combined computational modeling with microtomographic particle image velocimetry(uTPiM)to comprehensively characterize the 3D flow *** results corroborate the presence of a steady secondary flow,underscoring the effectiveness of our *** 3D spiral microfluidics platform paves the way for exploring intricate microflow dynamics,with promising applications in areas such as drug delivery,diagnostics,and lab-on-a-chip systems.

关键词： thereby straight twisted

Climate change impacts the distribution of Quercus section Cyclobalanopsis(Fagaceae),a keystone lineage in East Asian evergreen broadleaved forests

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Plant Diversity 2023年第5期45卷 552-568页

作者： Lin Lin Xiao-Long Jiang Kai-Qi guo amy Byrne Min Deng School of Ecology and Environmental Sciences Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of BiodiversityYunnan UniversityKunming 650500YunnanChina Laboratory of Ecology and Evolutionary Biology State Key Laboratory for Conservation and Utilization of BioResources in YunnanYunnan UniversityKunming 650500YunnanChina College of Forestry Central South University of Forestry and TechnologyChangsha 410004HunanChina Shanghai Chenshan Botanical Garden Shanghai 201602China The Morton Arboretum LileIL 60532-1293USA

East Asian evergreen broadleaved forests(EBFLs) harbor high species richness,but these ecosystems are severely impacted by global climate change and *** and managing EBLFs requires understanding dominant tree distribution *** this study,we used 29 species in Quercus section Cyclobalanopsis-a keystone lineage in East Asian EBLFs-as proxies to predict EBLF distribution dynamics using species distribution models(SDMs).We examined climatic niche overlap,similarity,and equivalency among seven biogeographical regions’ species using’ecospat’.We also estimated the effectiveness of protected areas in the predicted range to elucidate priority conservation *** results showed that the climatic niches of most geographical groups *** western species under the Indian summer monsoon regime were mainly impacted by temperature factors,whereas precipitation impacted the eastern species under the East Asian summer monsoon *** simulation predicted a northward range expansion of section Cyclobalanopsis between 2081 and 2100,except for the ranges of the three Himalayan species analyzed,which might shrink *** greatest shift of highly suitable areas was predicted for the species in the South Pacific,with a centroid shift of over 300 ***,only 7.56% of suitable habitat is currently inside protected areas,and the percentage is predicted to continue declining in the *** better conserve Asian EBLFs,establishing nature reserves in their northern distribution ranges,and transplanting the populations with predicted decreasing numbers and degraded habitats to their future highly suitable areas,should be high-priority objectives.

关键词： Species distribution model(SDM) East Asian tropics and subtropics Biodiversity conservation Distribution dynamics Global climate change

Refractory high-entropy alloys:A focused review of preparation methods and properties

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Journal of Materials Science & Technology 2023年第11期142卷 196-215页

作者： Wei Xiong amy X.Y.guo Shuai Zhan Chain-Tsuan Liu Shan Cecilia Cao Materials Genome Institute Shanghai UniversityShanghai 200444China Hong Kong Institute of Advanced Study City University of Hong KongKowloonHong KongChina

In recent years,high-entropy alloys(HEAs)have become prominent metallic materials due to their unique design strategies and excellent mechanical *** HEAs-inherent high-entropy,lattice-distortion,sluggish-diffusion,and cocktail effects make HEAs maintain high strength,oxidation resistance,corrosion resistance,wear resistance,and other excellent comprehensive properties,showing stronger competitiveness relative to traditional *** high-entropy alloys(RHEAs)are considered as a new kind of high-temperature materials with great application prospects due to their excellent mechanical properties and have the potential to replace nickel-based superalloy as the next generation of high-temperature *** reviewed the research status and preparation methods of RHEAs in recent years,including the metallurgical smelting,powder metallurgy,magnetron sputtering,and additive manufacturing *** microstructure and phase-transformation process of RHEAs were *** mechan-ical properties and main strengthening and toughening mechanisms of RHEAs,such as solid-solution strengthening,precipitation strengthening,and the transformation-induced plasticity(TRIP),were discussed,and the deformation mechanism of RHEAs was *** properties of RHEAs,including high strength,oxidation resistance,corrosion and wear resistance were *** will meet the huge market demand in the engineering materials field,but there are still many challenges,such as the tradeoff between high strength and high ductility,structural design,and performance optimization of RHEAs with brittle BCC *** believe that this combination of knowledge may shape the future of RHEAs and break through the mutually exclusive conundrum of high strength and high toughness for RHEAs.

关键词： Refractory high-entropy alloy Preparation technology Phase transformation Mechanical properties Strengthening and toughening mechanisms Deformation mechanisms

Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023

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The Crop Journal 2021年第1期9卷 143-153页

作者： Pingping Zhang Chenjin guo Zhao Liu amy Bernardo Hongxiang Maa Hongxiang Ma Peng Jiang Guicheng Song Guihua Bai Jiangsu Academy of Agricultural Sciences Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing 210014JiangsuChina Department of Agronomy Kansas State UniversityManhattanKS 66506USA Hebei Agricultural University Baoding 071001HebeiChina USDA-ARS Hard Winter Wheat Genetics Research UnitManhattanKS 66506USA

Fusarium head blight(FHB)is one of the prevalent fungal diseases of wheat *** new FHB resistance quantitative trait loci(QTL)in adapted wheat cultivars is a critical step for breeding new FHB-resistant *** this study,we developed a population of 236 F5:7 recombinant inbred lines(RILs)using two popular Chinese wheat cultivars,Yangmai 158 and Zhengmai 9023,with moderate FHB resistance to identify the QTL for FHB type II *** population was evaluated for percentage of symptomatic spikelets per spike(PSS)using single floret injection in repeated greenhouse *** PSSs were 33.2%for Yangmai 158 and 30.3%for Zhengmai 9023.A genetic linkage map of 1002 single nucleotide polymorphisms(SNPs)generated by genotyping-by-sequencing(GBS)was constructed for the RIL *** QTL were identified for FHB resistance,and three of them were repeatable in the both *** 9023 contributed the resistance allele at one repeatable QTL,designated as Qfhb.7D,whereas Yangmai 158 contributed the resistance alleles at the other two repeatable QTL,Qfhb.3AL and *** additional QTL,Qfhb.4AS was significant in the mean PSS,and Qfhb.2DL and Qfhb.7AS were significant in only one *** of each allele individually at the three repeatable QTL significantly changed ***.3AL,Qfhb.2DS,and Qfhb.7D explained 8.35%to 9.89%,5.13%to 7.43%,and 6.15%to 9.32%of the phenotypic variations,*** three repeatable QTL contributed by the two parents were additive and stacking the resistance alleles from all the three repeatable QTL showed the highest level of resistance in the current RIL *** SNPs in the QTL regions of Qfhb.3AL,Qfhb.2DS,and Qfhb.7D were converted into KBioscience competitive allele-specific PCR(KASP)*** KASP marker for Qfhb.3AL was validated in a panel of wheat cultivars from *** of these KASP markers could be useful for marker-assisted selection to stack these QTL.

关键词： QTL mapping FHB Yangmai 158 Zhengmai 9023 KASP

Biomedical applications of the powder-based 3D printed titanium alloys:A review

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Journal of Materials Science & Technology 2022年第30期125卷 252-264页

作者： amy X.Y.guo Liangjie Cheng Shuai Zhan Shouyang Zhang Wei Xiong Zihan Wang Gang Wang Shan Cecilia Cao Materials Genome Institute Shanghai UniversityShanghai 200444China

3D printing technology is a new type of precision forming technology and the core technology of the third industrial *** powder-based 3D printing technology of titanium and its alloys have received great attention in biomedical applications since its advantages of custom manufacturing,costsaving,time-saving,and resource-saving *** particular,the personalized customization of 3D printing can meet specific needs and achieve precise control of micro-organization and structural *** purpose of this review is to present the most advanced multi-material 3D printing methods for titanium-based *** first reviewed the bone tissue engineering,the application of titanium alloy as bone substitutes and the development of manufacturing technology,which emphasized the advantages of 3D printing technology over traditional manufacturing *** is more,the optimization design of the hierarchical structure was analyzed to achieve the best mechanical properties,and the biocompatibility and osseointegration ability of the porous titanium alloy after implantation in living bodies was ***,we emphasized the development of digital tools such as artificial intelligence,which provides new ideas for the rational selection of processing *** 3D printing titanium-based alloys will meet the huge market demand in the biomedical field,but there are still many challenges,such as the trade-off between high strength and low modulus,optimization of process parameters and structural *** believe that the combination of mechanical models,machine learning,and metallurgical knowledge may shape the future of metal printing.

关键词： The powder-based 3D printing technology Ti-based alloys Biomedical applications Artificial intelligence

Influence of starch sources and dietary protein levels on intestinal functionality and intestinal mucosal amino acids catabolism in broiler chickens

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Journal of Animal Science and Biotechnology 2019年第3期10卷 658-672页

作者： Dafei Yin Peter H.Selle amy F.Moss Youli Wang Xiaoyu Dong Zhibin Xiao Yuming guo Jianmin Yuan State key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China Poultry Research Foundation The University of SydneyWerombi RoadCamdenNSW 2570Australia

Background: There is growing interest in carbohydrate and protein nutrition to enhance the efficiency of animal ***-crude protein diets depress environmental pollution and feeding cost,but the challenge to their adoption is maintaining digestive function and growth performance of *** present study was conducted to evaluate the influence of different dietary starch sources and protein levels on intestinal functionality and mucosal amino acid ***: Six dietary treatments,based on maize and soybean meal,were offered to 360 AA+male chicks from 6 to 35 d post-hatch as a 3 × 2 factorial *** waxy rice or amylose was added to a conventional maize-soy diet to provide three sources of starch with different digestion rates and relatively high and low dietary protein *** performance,parameters of intestinal functionality and concentrations of free amino acid in the portal circulation were ***: In the grower phase,starch source influenced(P amylose supported significantly higher weight gains than waxy *** increase of ileal ATP concentrations and Na^+/K^+-ATPase activity were found in amylose ***,amylose decreased BrdU positive cell numbers and down-regulated m RNA expression for *** activity in the ileum was higher(P amylose,***: Amino acid catabolism in the gut mucosa is subject to nutritional *** that amino acids can be spared from catabolism in the gut mucosa by supplementation of amylose,it follow

关键词： Amino acids Broiler chickens Enterocytes Glucose Starch

An intersegmental single-cell profile reveals aortic heterogeneity and identifies a novel Malat1+vascular smooth muscle subtype involved in abdominal aortic aneurysm formation

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1817-1830页

作者： Liwen Yu Jie Zhang amy Gao Meng Zhang Zunzhe Wang Fangpu Yu Xiaobin guo guohai Su Yun Zhang Meng Zhang Cheng Zhang The Key Laboratory of Cardiovascular Remodeling and Function Research Chinese Ministry of EducationChinese National Health Commission and Chinese Academy of Medical SciencesThe State and Shandong Province Joint Key Laboratory of Translational Cardiovascular MedicineDepartment of CardiologyQilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Cardiovascular Disease Research Center of Shandong First Medical University Central Hospital Affiliated to Shandong First Medical UniversityJinanChina

The developmental origin,anatomical location,and other factors contribute to aortic heterogeneity in a physiological *** this basis,vascular diseases occur at different ratios based on position specificity,even with the same risk ***,the continuous intersegmental aortic profile has been rarely reported at the single-cell *** reveal aortic heterogeneity,we identified 15 cell subtypes from five continuous aortic segments by marker genes and functional definitions.

关键词： aortic segmental specificity

The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair

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Bone Research 2024年第2期12卷 289-301页

作者： Heeseog Kang amy L.Strong Yuxiao Sun Lei guo Conan Juan Alec C.Bancroft Ji Hae Choi Chase A.Pagani Aysel A.Fernandes Michael Woodard Juhoon Lee Sowmya Ramesh Aaron W.James David Hudson Kevin N.Dalby Lin Xu Robert J.Tower Benjamin Levi Center for Organogenesis Regeneration and TraumaDepartment of SurgeryUniversity of Texas SouthwesternDallasTX 75390USA Section of Plastic Surgery Department of SurgeryUniversity of MichiganAnn ArborMI 48109USA Quantitative Biomedical Research Center Peter O’Donnell Jr.School of Public HealthUniversity of Texas SouthwesternDallasTX 75390USA Department of Orthopedics and Sports Medicine University of WashingtonSeattleWA 98195USA Division of Chemical Biology and Medicinal Chemistry College of PharmacyUniversity of Texas at AustinAustinTX 78712USA Department of Pathology Johns Hopkins UniversityBaltimoreMD 21218USA

While hypoxic signaling has been shown to play a role in many cellular processes,its role in metabolism-linked extracellular matrix(ECM)organization and downstream processes of cell fate after musculoskeletal injury remains to be ***(HO)is a debilitating condition where abnormal bone formation occurs within extra-skeletal *** andhypoxia-inducible factor 1α(HIF-1α)activation have been shown to promote ***,the underlying molecular mechanisms bywhich the HIF-1αpathway in mesenchymal progenitor cells(MPCs)contributes to pathologic bone formation remain to ***,we used a proven mouse injury-induced HO model to investigate the role of HIF-1αon aberrant cell ***-cell RNA sequencing(scRNA-seq)and spatial transcriptomics analyses of the HO site,we found that collagen ECM organizationis the most highly up-regulated biological process in *** mesenchymal cell-specific deletion of Hif1α(Hoxa11-CreER^(T2);Hif1a^(fl/fl))significantly mitigated HO in ***-seq analysis of these Hoxa11-CreER^(T2);Hif1a^(fl/fl)mice identified the PLOD2/LOXpathway for collagen cross-linking as downstream of the HIF-1αregulation of ***,our scRNA-seq data and mechanisticstudies further uncovered that glucose metabolism in MPCs is most highly impacted by HIF-1α*** a translational aspect,a pan-LOX inhibitor significantly decreased HO.A newly screened compound revealed that the inhibition of PLOD2 activity in MPCssignificantly decreased osteogenic differentiation and glycolytic *** suggests that the HIF-1α/PLOD2/LOX axis linked tometabolism regulates HO-forming MPC *** results suggest that the HIF-1α/PLOD2/LOX pathway represents a promisingstrategy to mitigate HO formation.

关键词： metabolism skeletal aberrant