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Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

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Science China(Life Sciences) 2011年第2期54卷 112-120页

作者： gao Jun gao guolan ZHANG YanYu WANG Fen Department of Gynecology and Obstetrics the First Affiliated Hospital of Nanchang University Nanchang 330006 China Department of Gynecology Aviation General Hospital Beijing 100012 China

This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and ***-OV-3 cells were injected subcutaneously into nude *** stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days,while the control group was *** mice were sacrificed on day 36 after SK-OV-3 cell injection,and tumors were *** tissues were processed for 2D gel electrophoresis,mass spectrometry(nanoUPLC-ESI-MS/MS) and Western *** expression of 20 proteins was found to be significantly altered between the stress and control groups,of which 14 were up-regulated,five were down-regulated,and one protein was found only in the stress *** proteins were identified by UPLC-ESI-MS/MS,and Western blotting results were consistent with those of proteomic *** present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.

关键词： proteomic psychological stress ovarian cancer 2D electrophoresis UPLC-ESI-MS/MS

Epigenetic reprogramming of embryos derived from sperm frozen at-20℃

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Science China(Life Sciences) 2012年第4期55卷 349-357页

作者： CHAO ShiBin LI JianChun JIN XuanJin TANG HaiXun WANG GongXian gao guolan Center of Reproductive Medicine the First Affiliated Hospital of Nanchang University Nanchang 330006 China Department of Obstetrics and Gynecology Aviation General Hospital of China Beijing 100101 China

Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection （ICSI） is much needed. It has been shown previously that spermatozoa frozen at -20~C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at -20~C without cryoprotectants. The results showed that alt- hough both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramrning of embryos derived from fresh sperm. The results re- ported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.

关键词： spermatozoa freezing ICSI epigenetic histone modification DNA methylation

低氧诱导因子-1α RNAi表达质粒对卵巢癌细胞影响的实验研究

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中国肿瘤临床 2011年第9期38卷 485-487页

作者：聂春莲高国兰重庆市妇幼保健院妇科重庆市400013 南昌大学第一附属医院妇产科

目的探讨人HIF-1α短发夹(sh)RNA表达质粒对卵巢癌SKOV3细胞HIF-1α基因的表达及对紫杉醇敏感性的影响。方法构建HIF-1α(sh)RNA表达质粒,脂质体法转染,RT-PCR和Western blot检测癌细胞HIF-1α mRNA和蛋白表达水平,MTT法检测癌细胞对紫... 详细信息

目的探讨人HIF-1α短发夹(sh)RNA表达质粒对卵巢癌SKOV3细胞HIF-1α基因的表达及对紫杉醇敏感性的影响。方法构建HIF-1α(sh)RNA表达质粒,脂质体法转染,RT-PCR和Western blot检测癌细胞HIF-1α mRNA和蛋白表达水平,MTT法检测癌细胞对紫杉醇的敏感性。结果转染质粒48 h后,H质粒转染组SKOV3细胞紫杉醇作用24 h的IC_(50)值明显降低(P<0.05),同时P-糖蛋白表达水平显著降低,差异有统计学意义(F=7.24,P=0.01)。结论:针对HIF-1α的RNAi表达质粒可下调低氧培养的卵巢癌)胞SKOV3 HIF-1α基因mRNA和蛋白及P-gp的表达,从而提高癌细胞对紫杉醇的敏感性。

关键词：低氧RNA干扰低氧诱导因子-1α 卵巢肿瘤 P-糖蛋白

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外阴上皮内瘤变的病理及临床处理

中国肿瘤临床 2009年第8期36卷 234-237,239页

作者：聂春莲高国兰南昌大学第一附属医院妇产科南昌市330006

外阴上皮内瘤变(VIN)是外阴癌的癌前病变,正确诊治VIN具有重要意义。由于1986年国际外阴疾病研究协会(ISSVD)对VIN的分类存在诸多问题,2004年ISSVD采用了新的分类系统,将VIN分为2类病变:普通型和分化型疣样型VIN主要病理特征有基底层区... 详细信息

外阴上皮内瘤变(VIN)是外阴癌的癌前病变,正确诊治VIN具有重要意义。由于1986年国际外阴疾病研究协会(ISSVD)对VIN的分类存在诸多问题,2004年ISSVD采用了新的分类系统,将VIN分为2类病变:普通型和分化型疣样型VIN主要病理特征有基底层区或基底层旁区的非典型鳞状细胞胞浆极少;基底细胞样型VIN主要病理特征有表皮由外形较一致的未分化的不典型基底样细胞构成;分化型VIN主要病理特征有在分化完全的外阴上皮细胞背景中出现不典型细胞,基底层及基底层旁具有过早角化的鳞状细胞、角化不全细胞,表皮突中可出现角化珠样改变。VIN的诊断必须根据活检病理检查才能明确 VIN可选用的治疗方法包括手术治疗、物理治疗、局部药物治疗等,以手术为主,选用治疗方法时需采用个体化的治疗措施。

关键词：外阴上皮内瘤样病变病理治疗

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老年女性子宫颈癌筛查中国专家共识

现代肿瘤医学 2024年第13期32卷 2313-2317页

作者：李克敏李乔高国兰尹如铁程静新蔡红兵中国老年学和老年医学学会妇科分会四川大学华西第二医院四川成都610016 同济大学东方医院上海200120 武汉大学中南医院湖北武汉430071 不详

中国老年女性因各种因素的影响,子宫颈癌筛查的比率低,导致原本可防可治的子宫颈癌成为威胁其生命健康的主要危险因素之一。为规范中国老年女性子宫颈癌筛查,指导临床实践,中国老年学和老年医学学会妇科分会组织专家依据循证医学证据,... 详细信息

中国老年女性因各种因素的影响,子宫颈癌筛查的比率低,导致原本可防可治的子宫颈癌成为威胁其生命健康的主要危险因素之一。为规范中国老年女性子宫颈癌筛查,指导临床实践,中国老年学和老年医学学会妇科分会组织专家依据循证医学证据,结合临床诊疗经验,形成以下中国专家共识,用以逐渐规范我国老年子宫颈癌防控。

关键词：老年女性子宫颈癌筛查中国专家共识

卵巢癌治疗新靶标:Hedgehog信号通道

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肿瘤防治研究 2014年第8期41卷 948-952页

作者：陈琦高国兰南昌大学第二附属医院妇产科南昌330006 北京航空总医院妇产科

上皮性卵巢癌是卵巢恶性疾病中最常见的类型。在美国,卵巢癌是最致命的妇科恶性疾病。到目前为止,即使采取彻底的卵巢肿瘤细胞减灭术配合有效的术后化疗,临床预后仍然不尽人意。近来,我们逐渐认识到,在许多情况下,调控不同组织发育过程... 详细信息

上皮性卵巢癌是卵巢恶性疾病中最常见的类型。在美国,卵巢癌是最致命的妇科恶性疾病。到目前为止,即使采取彻底的卵巢肿瘤细胞减灭术配合有效的术后化疗,临床预后仍然不尽人意。近来,我们逐渐认识到,在许多情况下,调控不同组织发育过程的细胞信号通道及信号分子是相类似的。当调控机制紊乱时,这些信号通道及信号分子将促使各种肿瘤形成及癌症发生。在这里,我们将讨论Hedgehog信号通道与卵巢正常生物学及癌症发生的相关性。通过一些体内外实验,我们已经逐步探索出Hedgehog信号通道促使卵巢形成及生长的分子机制。基于近年的研究成果,我们推断Hedgehog信号的抑制可以干预卵巢癌球样结构的形成。该信号通道的信号分子可能将成为一系列重要的药物治疗靶标,成为晚期卵巢癌重要的联合治疗方案的战略选择。

关键词： Hedgehog信号通道卵巢癌靶向治疗

Proteomic Analysis of Human Epithelial Ovarian Cancer Xenografts in Immunodeficient Mice Exposed to Chronic Psychological Stress

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Proteomic Analysis of Human Epithelial Ovarian Cancer Xenogr...

nm23及NDRG1蛋白在卵巢癌肿瘤抑郁模型中的表达

第十三届中国科协年会第18分会场-癌症流行病趋势和防控策略研讨会

作者： Jun gao, guolan gao*, Lijuan XIONG, Yanyu ZHANG, Fen WANG (Department of Gynecology, Aviation General Hospital, Beijing 100012, China)

To compare alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice. Aslo, to explore the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression. Methods: SK-OV-3 cells were injected subcutaneously into nude mice. The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days, while the control group was unrestrained. All mice were sacrificed on Day 36 after SK-OV-3 cell injection, and tumors were excised. Tumor tissues were processed for 2D gel electrophoresis, mass spectrometry (nanoUPLC-ESI-MS/MS) and western blotting. Results: The expression of twenty proteins was found to be significantly altered between the stress and control groups, of which fourteen were up-regulated, five were down-regulated, and one protein was found only in the stress group. All proteins were identified by UPLC-ESI-MS/MS, and western blotting results were consistent with those of proteomic methods. Conclusions: The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.

关键词： proteomic psychological stress ovarian cancer 2-D electrophoresis UPLC -ESI-MS/MS

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中国肿瘤临床 2010年第18期37卷 1039-1041,1049页

作者：高军高国兰张艳玉王芬南昌大学第一附属医院妇产科南昌市330006 中国航空工业集团中心医院妇科

目的:探讨不良心理应激影响肿瘤发生发展的分子学机制。方法:采用蛋白组学方法筛选、鉴定荷卵巢癌裸鼠不良心理应激模型移植瘤中的差异表达蛋白,并用Western Blot验证;用免疫组化SP法检测25例应激组和20例对照组移植瘤中的nm23及NDRG1... 详细信息

目的:探讨不良心理应激影响肿瘤发生发展的分子学机制。方法:采用蛋白组学方法筛选、鉴定荷卵巢癌裸鼠不良心理应激模型移植瘤中的差异表达蛋白,并用Western Blot验证;用免疫组化SP法检测25例应激组和20例对照组移植瘤中的nm23及NDRG1蛋白的表达特点。结果:两组在p17.1、Mr=21ku和p15.5、Mr=43ku处有两个显著差异点,前一个在应激组表达上调而后一个表达下调,通过质谱分别鉴定为nm23及NDRG1蛋白;Western Blot验证结果同蛋白组学的一致;免疫组化显示nm23蛋白在应激组和对照组中阳性表达率分别为100%和70%,NDRG1蛋白为56%和85%,差异均有统计学意义(P<0.05)。结论:不良心理应激可能通过激活癌基因和失活抑癌基因来影响肿瘤的发生发展,nm23及NDRG1可作为基因研究和基因治疗的靶点。

关键词：不良心理应激卵巢癌 nm23蛋白 NDRG1蛋白免疫组织化学

HPV16 E6 E7 siRNA作用于人宫颈癌细胞株的实验研究

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中国肿瘤临床 2008年第21期35卷 1236-1240页

作者：高国兰韩洁聂春莲南昌大学第一附属医院妇产科南昌市330006 江西省肿瘤医院妇瘤科

HPV1摘6E要6、E7目原的癌:使基用因的HP特V异16性E6抑、E制7作siR用N,A为处探理讨表使达用HsPiRVN1A6阳治性疗宫的颈人癌宫的颈可鳞行癌性细提胞供株实Ca验Sk基i和础S。iH方a,法观:察分其别对设计并合成靶向于HPV16E6、E7的siRNA各3条,... 详细信息

HPV1摘6E要6、E7目原的癌:使基用因的HP特V异16性E6抑、E制7作siR用N,A为处探理讨表使达用HsPiRVN1A6阳治性疗宫的颈人癌宫的颈可鳞行癌性细提胞供株实Ca验Sk基i和础S。iH方a,法观:察分其别对设计并合成靶向于HPV16E6、E7的siRNA各3条,经脂质体包裹后转染两株细胞,分别采用RT-PCR和WesternBlot方s果iR:法N同A,通对组过照的对组靶转相基染比因前,表各后达E各水6、时平E7间则s无段iRN明两A显基均变因可化m引R。起N其A靶中及基蛋E因6白-表1的s达i表R水N达A平情和的况E显7的-著2检s性i测R下N,A验降对(证P靶R<0基N.0A因5i)作抑;而用制空的作脂特用质异较体性强组及。及时E6阴效-1性性si对。RN照结A和E7-2siRNA转染两株细胞后24h、48h、72h及96h均可观察到靶基因表达的显著性降低(P<0.05),而对相应的非靶基因无明显抑制作用。结论:不同序列的E6、E7siRNA,对靶基因的干扰效率不同;E6、E7siRNA分别作用于宫颈鳞癌细胞株,均引起了靶基因的特异、高效性的抑制,而对非靶基因的表达无明显变化,且抑制作用至少维持到转染后关9键6h词。为s进iR一NA步研E6究采E用7RHAPNV干16扰技宫术颈进癌行宫颈癌的生物治疗奠定了基础。

关键词： siRNA E6 E7 HPV16 宫颈癌