Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

作     者：GAO Jun GAO GuoLan ZHANG YanYu WANG Fen 

作者机构：Department of Gynecology and Obstetrics the First Affiliated Hospital of Nanchang University Nanchang 330006 China Department of Gynecology Aviation General Hospital Beijing 100012 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2011年第54卷第2期

页      面：112-120页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071006[理学-神经生物学] 

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 30860301 and 30660192) 

主　　题：proteomic psychological stress ovarian cancer 2D electrophoresis UPLC-ESI-MS/MS 

摘      要：This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and ***-OV-3 cells were injected subcutaneously into nude *** stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days,while the control group was *** mice were sacrificed on day 36 after SK-OV-3 cell injection,and tumors were *** tissues were processed for 2D gel electrophoresis,mass spectrometry(nanoUPLC-ESI-MS/MS) and Western *** expression of 20 proteins was found to be significantly altered between the stress and control groups,of which 14 were up-regulated,five were down-regulated,and one protein was found only in the stress *** proteins were identified by UPLC-ESI-MS/MS,and Western blotting results were consistent with those of proteomic *** present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.