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Chlorogenic acid complex(CgA7),standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells

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Food Science and Human Wellness 2017年第3期6卷 147-153页

作者： K.gouthamchandra H.V.Sudeep b.J.Venkatesh K.Shyam Prasad Department of biomedicinal Research R&D Centre for ExcellenceVidya Herbs Pvt.Ltd#14AJigani I PhaseBangalore 560105KarnatakaIndia

Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial *** acids(CgAs)are main ingredient of coffee beans which has been extensively used in nutraceuticals and ***,various therapeutic effects of chlorogenic acids have been ***,there are limited studies to investigate its anticancer *** the present study,we have used chlorogenic acid complex(CgA7)a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different *** our results we found CgA7 treatment induces cell death in a dose and time dependent manner in different cancer cell ***,CgA7 induced apoptosis was characterized by dna fragmentation,PARP-1 cleavage,caspase-9 activation,and down regulation of bcl-2,an anti-apoptotic protein and up regulation of pro-apoptotic protein *** findings indicated that CgA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.

关键词： Apoptosis Chlorogenic acid complex PARP-1 green coffee beans dna fragmentation

Diabetes--Role of epigenetics,genetics,and physiological factors

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中南大学学报（医学版） 2009年第9期34卷 837-845页

作者： Sreerama Krupanidhi Saikiran K. Sedimbi ganesan Vaishnav Sreerama Sai Madhukar Carani b. Sanjeevi Department of biosciences Sri Sathya Sai University Prasanthi Nilayam 515134 India Department of Molecular Immunogenetics Karolinska Institute Stockholm 17176 Sweden Department of bioinformatics Sathyabama University Chennai 600019 India

Cells of organ systems are endowed with a relatively similar genome while epigenome niches keep varying chronologically and defined explicitly in the respective *** genome of an individual is always influenced by parental,embryonic,tissue-specific,and environmental epigenomes and the same must have been the possible reason for invariable inquiries relating to familial,environmental and life style patterns in the preliminary investigations of diabetic *** methylation of lysine residues of histones and cytosines of Cpg islands of promoter dna impede the transcription of genes and homocysteine is the metabolic key player of methyl *** and COX7A1 are the 2 examples in the present review to elucidate the epigenetic influence on the onset of *** are additional promising cellular components influencing both at transcriptional and translational levels and promoting either in favour or against(i.e.,feed back) TFs,signaling factors and proteins through their pliotropic effects and thus are reported to regulate cellular ***-124a and miR-9 are primarily endemic to nervous tissue and they are now being exploited in islets for their function in executing exocytosis of insulin,which of course is one of the fundamental canons of ***-375 persuades beta cells for glucose-induced insulin gene *** current approach to evaluate the constellation of genes and their products involved in diabetes in huge number of samples through gWA studies may unravel intricacies involved in the management of diabetes and its associated consequences.

关键词：糖尿病临床治疗疗效遗传学

blastocystis in ulcerative colitis patients:genetic diversity and analysis of laboratory findings

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Asian Pacific Journal of Tropical Medicine 2016年第9期9卷 893-896页

作者： Adil Coskun Erdogan Malatyali Hatice Ertabaklar Mustafa b Yasar Ali O Karaoglu Sema Ertug Department of gastroenterology Faculty of MedicineAdnan Menderes University Department of Parasitology Faculty of MedicineAdnan Menderes University

Objective:To determine blastocystis frequency and subtypes(ST) in ulcerative colitis(UC) patients and analyse some laboratory findings between blastocystis positive and negative ***:Faecal samples from 150 UC patients in Adnan Menderes University,Training and Research Hospital were examined by direct microscopy and cultivated in Jones *** positive cultures were subjected to dna isolation and subtypes were identified by sequencing of barcode region.A retrospective analysis was conducted on C reactive protein(CRP),leukocyte counts(WbC),neutrophil counts,and sedimentation ***:The overall positive rate of blastocystis was 8%(12 patients) and the most abundant subtype was ST3(eight isolates,66.7%),followed by ST1,ST2 and *** findings between blastocystis infected and non-infected UC patients were not significantly *** was 3.8% among the patients in active stage,while it was 11.8% among the patients in remission ***:The present study confirms previous findings that have indicated the predominance of blastocystis ST3 in humans and contributes additional evidence that suggests the low colonization of blastocystis infection in ulcerative colitis patients during active stage.

关键词： blastocystis Ulcerative colitis Subtype Laboratory findings

配合物[Ni(IDb)_2]_2·(SO_4)_2·3CH_3CH_2OH·CH_3OH·5H_2O的合成、晶体结构及其荧光光谱的研究

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化学学报 2008年第14期66卷 1651-1657页

作者：陈彦国何治柯张斓陈琼陆江林邓真丽陈灵王卫东湖北师范学院生化分析技术湖北省重点实验室武汉大学化学与分子科学学院武汉430072 湖北师范学院化学与环境工程学院黄石435002

标题配合物是由三齿配体N,N-二(2-苯并咪唑甲基)亚胺(IDb)与NiSO4?6H2O在甲醇-乙醇溶液中反应得到的紫色晶体.X射线衍射测定了其单晶结构.结构分析表明,镍(II)分别与两个IDb配体中的苯并咪唑的四个氮和胺基的两个氮配位形成畸变的八面... 详细信息

标题配合物是由三齿配体N,N-二(2-苯并咪唑甲基)亚胺(IDb)与NiSO4?6H2O在甲醇-乙醇溶液中反应得到的紫色晶体.X射线衍射测定了其单晶结构.结构分析表明,镍(II)分别与两个IDb配体中的苯并咪唑的四个氮和胺基的两个氮配位形成畸变的八面体构型.研究了纳米金和小牛胸腺dna对配合物荧光的影响,探讨了配合物的荧光猝灭与恢复的可能机理.

关键词：镍配合物晶体结构荧光光谱纳米金 dna

Melatonin prevents oxidative stress,inflammatory activity,and dna damage in cirrhotic rats

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World Journal of gastroenterology 2022年第3期28卷 348-364页

作者： Josieli R Colares Renata M Hartmann Elizângela g Schemitt Sandielly R b Fonseca Marilda S brasil Jaqueline N Picada Alexandre S Dias Aline F bueno Cláudio A Marroni Norma P Marroni Medical Sciences Program Universidade Federal do Rio Grande do SulPorto Alegre 90050-170Brazil Marilda S BrasilNorma biological Sciences Program Universidade Federal do Rio Grande do SulPorto Alegre 90050-170Brazil Cellular and Molecular biology Program Lutheran University of Brazil(ULBRA)Canoas 92425-900Brazil Pneumological Sciences Program Universidade Federal do Rio Grande do SulPorto Alegre 90050-170Brazil Posgraduate Program in Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90050-170Brazil

bACKgROUND Cirrhosis is an important health problem characterized by a significant change in liver *** animals,this can be reproduced by an experimental model of bile duct ligation(bDL).Melatonin(MLT)is a physiological hormone synthesized from serotonin that has been studied for its beneficial properties,including its antioxidant *** To evaluate MLT’s effects on oxidative stress,the inflammatory process,and dna damage in an experimental model of secondary biliary *** Male Wistar rats were divided into 4 groups:Control(CO),CO+MLT,bDL,and bDL+*** was administered(20 mg/kg)daily beginning on day 15 after biliary *** day 29 the animals were *** samples,liver tissue,and bone marrow were collected for further *** bDL caused changes in biochemical and histological parameters and markers of inflammatory *** acid(0.46±0.01)reactive substance levels,superoxide dismutase activity(2.30±0.07)and nitric oxide levels(2.48±0.36)were significantly lower(Pgroups that received *** damage was also lower(Pgroups(171.6±32.9)than the bDL-only group(295.5±34.8).Tissue damage and the expression of nuclear factor kappa b,interleukin-1β,Nrf2,NQO1 and Hsp70 were significantly lower in animals treated with MLT(PbDL-induced secondary biliary cirrhosis,MLT effectively restored the evaluated parameters.

关键词： Antioxidants Secondary biliary cirrhosis Oxidative stress Melatonin bile duct ligation

global methylation status of sperm dna in carriers of chromosome structural aberrations

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Asian Journal of Andrology 2017年第1期19卷 117-124页

作者： Marta Olszewska Miroslawa Z barciszewska Monika Fraczek Nataliya Huleyuk Vyacheslav b chernykh Danuta Zastavna Jan barciszewski Maciej Kurpisz Department of Reproductive biology and Stem Cells Institute of Human Genetics Polish Academy of Sciences Strzeszynska 32 60-479 Poznan Poland Department of RNA biology Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14 61-704 Poznan Poland Institute of Hereditary Pathology Ukrainian Academy of Medical Sciences Lysenko Street 31a 79000 Lviv Ukraine Research Centre for Medical genetics Russian Academy of Medical Sciences Moskvorechie Street 1115478 Moscow Russian Federation.

Male infertility might be clearly associated with aberrant dna methylation patterns in human spermatozoa. An association between oxidative stress and the global methylation status of the sperm genome has also been suggested. The aim of the present study was to determine whether the global sperm dna methylation status was affected in the spermatozoa of carriers of chromosome structural aberrations. The relationships between the 5-methylcytosine （msC） levels in spermatozoa and chromatin integrity status were evaluated. The study patients comprised male carriers of chromosome structural aberrations with reproductive failure （n = 24）, and the controls comprised normozoospermic sperm volunteers （n = 23）. The global msC level was measured using thin-layer chromatography （TLC） and immunofluorescence （IF） techniques. The sperm chromatin integrity was assessed using aniline blue （Ab） staining and TUNEL assay. The mean msC levels were similar between the investigated chromosome structural aberrations carriers （P） and controls （K）. However, sperm chromatin integrity tests revealed significantly higher values in chromosomal rearrangement carriers than in controls （P 〈 0.05）. Although the potential relationship between sperm chromatin integrity status and sperm dna fragmentation and the msC level juxtaposed in both analyzed groups （P vs K） was represented in a clearly opposite manner, the low chromatin integrity might be associated with the high hypomethylation status of the sperm dna observed in carriers of chromosome structural aberrations.

关键词： 5-methylcytosine chromatin integrity chromosomal rearrangement chromosome translocation global dna methylation male infertility

Programmed cell death features in apple suspension cells under low oxygen culture

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2004年第2期5卷 9-15页

作者：徐昌杰,陈昆松,FERgUSON Ian b Laboratory of Fruit Molecular Physiology and biotechnology / The State Agriculture Ministry Laboratory of Horticultural Plant growth Development & biotechnology Zhejiang University Hangzhou 310029 China Laboratory of Fruit Molecular Physiology and biotechnology / The State Agriculture Ministry Laboratory of Horticultural Plant growth Development & biotechnology Zhejiang University Hangzhou 310029 China The Horticulture and Food Research Institute of New Zealand Private bag 92 169 Auckland New Zealand

Suspension-cultured apple fruit cells (Malus pumila Mill. cv. braeburn) were exposed to a low oxygen atmos-phere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Pro-toplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). dna breakdown and phosphatidyl-serine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.

关键词： Apple Programmed cell death Low oxygen

Improved resolution of mixed STR profiles using a fully automated differential cell lysis/dna extraction method

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Forensic Sciences Research 2020年第2期5卷 106-112页

作者： Matthew C.goldstein Jordan O.Cox Lori b.Seman Tracey Dawson Cruz Department of Forensic Science Virginia Commonwealth UniversityRichmondVirginiaUSA

Sexual assault evidence often contains sperm cells,which are typically separated from nonsperm cells using manual differential lysis *** goal of this study was to evaluate the automated QIAgEN QIAcube for this purpose and to compare it to manual QIAgEN and manual organic differential methods using dna yields and STR profile data for *** yields were determined by qPCR,followed by multiplex STR amplification,CE analysis,and mixture *** automated method was capable of effective cell separation,producing dna yields sufficient for STR ***,sperm fraction human:male dna ratios from the QIAcube samples were consistently closer to the desired 1:1 and STR profiles were less likely to result in mixtures,with 6–8fewer female alleles detected(median 1.5 alleles).Ultimately,using the QIAcube for automated differential processing of semen-containing mixtures reduces the need for downstream mixture interpretation and improves STR profile quality with substantially less hands-on time.

关键词： Forensic sciences forensic genetics sexual assault evidence QIAcube differential lysis sperm lysis automated dna extraction mixture analysis

Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

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Journal of Integrative Plant biology 2009年第11期51卷 982-992页

作者： Shaohong Qu Jong-Seong Jeont Pieter b.F. Ouwerkerk Maria bellizzi Jan Leach Pamela Ronald guo-Liang Wang Department of Plant Pathology Ohio State University Columbus OH 43210 USA Institute of Virology and biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Department of Plant Pathology University of California Davis CA 95616 USA graduate School of biotechnology and Plant Metabolism Research Center Yongin 446-701 Korea Institute of biology Leiden University Clusius Laboratory RA Leiden 2300 The Netherlands bioagdcultural Sciences and Pest Management Colorado State University Fort Collins CO 80523 USA Crop gene Engineering Key Laboratory of Hunan Province and Pre-State Key Laboratory of Crop germplasm Renovation and Resource Utilization Hunan Agricultural University Changsha 410128 China

Transposons are effective mutagens alternative to T-dna for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches：（i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/gal4 dna-binding domain （gVg） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,

关键词： Ac-Ds transposable element glucocorticoid binding domain/VP16 acidic activation domain/gal4 dna-binding domain-inducible expression Cre-lox site-specific recombination rice.