Programmed cell death features in apple suspension cells under low oxygen culture

作     者：徐昌杰,陈昆松,FERGUSON Ian B 

作者机构：Laboratory of Fruit Molecular Physiology and Biotechnology / The State Agriculture Ministry Laboratory of Horticultural Plant Growth Development & Biotechnology Zhejiang University Hangzhou 310029 China Laboratory of Fruit Molecular Physiology and Biotechnology / The State Agriculture Ministry Laboratory of Horticultural Plant Growth Development & Biotechnology Zhejiang University Hangzhou 310029 China The Horticulture and Food Research Institute of New Zealand Private Bag 92 169 Auckland New Zealand 

出 版 物：《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 (浙江大学学报A辑(应用物理与工程)(英文版))

年 卷 期：2004年第5卷第2期

页      面：9-15页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：Project supported by the National Basic Research Program (973) of China (No. G2000046806) the National Natural Science Foun-dation of China (No. 30170660) and Zhejiang Provincial Natural Science Foundation (No. ZD0004) and was also a part of co-op 

主　　题：Apple Programmed cell death Low oxygen 

摘      要：Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmos-phere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Pro-toplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidyl-serine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.