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中华微生物学和免疫学杂志 2003年第5期23卷 359-363页

作者：叶凯李其岚徐洪 kironde fred Alexander S 马辉文武汉大学药学院武汉大学生命科学学院 CRID Projects Department of BiochemistryMedical Schoolof Makerere University

目的　研究恶性疟原虫Pf70DNA片段作为DNA疫苗候选抗原基因的可能性。方法　应用PCR方法从含有恶性疟原虫Pf70基因DNA片段的质粒pGEX Pf70中扩增出包含Pf70DNA片段的长度为 95 7bp的PCR产物。将其插入带有乙肝表面抗原基因的真核表达载... 详细信息

目的　研究恶性疟原虫Pf70DNA片段作为DNA疫苗候选抗原基因的可能性。方法　应用PCR方法从含有恶性疟原虫Pf70基因DNA片段的质粒pGEX Pf70中扩增出包含Pf70DNA片段的长度为 95 7bp的PCR产物。将其插入带有乙肝表面抗原基因的真核表达载体pCMV S中 ,构建出重组质粒pCMV S Pf70。用提纯的重组质粒pCMV S Pf70作为DNA免疫制剂 ,以质粒pCMV SDNA和经过谷胱甘肽亲和层析法纯化的融合蛋白GST Pf70作为对照 ,免疫昆明种小白鼠。每隔 14d加强免疫1次 ,加强免疫 2次后第 7天采小鼠全血 ,用流式细胞仪检测CD8+ T淋巴细胞和CD4 + T淋巴细胞的水平 ,以检测体液免疫和细胞免疫状况。结果　接种了重组质粒pCMV S Pf70小鼠CD8+ T淋巴细胞的绝对量增加 ,其百分比含量增加了 39% ;CD4 + T淋巴细胞的相对含量保持恒定 ,绝对量稍有增加。用ELISA法分别检测经重组质粒pCMV S Pf70免疫的小鼠和经融合蛋白GST Pf70免疫的小鼠血清中抗Pf70蛋白质的抗体滴度 ,发现前者抗体滴度约为 1∶80 0 ,远远低于后者的滴度 (1∶5 0 0 0 )。研究结果证明 ,重组质粒pCMV S Pf70DNA可以诱导小鼠产生很强的细胞免疫和相对于蛋白质免疫而言较弱的体液免疫 ;Pf70蛋白质可以诱导小鼠产生很强的体液免疫。

关键词：疟疾恶性疟原虫 Pf70基因 DNA疫苗

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Hematology and blood serum chemistry reference intervals for children in Iganga district of Uganda

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Health 2013年第8期5卷 1261-1267页

作者： fred kironde Musa Sekikubo Halima Naiwumbwe Fatuma Namusoke William Buwembo Steven Kiwuwa Brenda Oketch Ramadhani Noor Roma Chilengi Edison Mworozi Mark Kaddumukasa African Malaria Network Trust Dar-es Salaam Tanzania Centre for Infectious Disease Research in Zambia (CIDRZ) Lusaka Zambia Makerere University College of Health Sciences Kampala Uganda

In this study, normal ranges for hematology and serum biochemistry in children aged 1 to 5 years inUgandawere determined. By a cross-sectional study, 1168 children from Iganga, a prospective site for clinical trials in Uganda, were screened. From 1168 households, 460 children were selected for enrollment, while 600 (58%) were excluded because of either a history of fever in the previous 24 hours, presence of asexual malaria parasites in the peripheral blood or presence of fever. Accordingly, 460 children (39.4%) of median age = 3 years were enrolled in the baseline study. While the lower limits of hemoglobin, hematocrit levels, mean corpuscular volume and platelet counts for the Ugandan children were found to be less than conventional reference values of Caucasisan children, the white blood cell count reference values were higher than the international intervals. On the other hand, the upper limits of the reference intervals for serum transaminases, bilirubin, creatinine, urea, total protein and albumin in sera of the Ugandan children were higher than the corresponding values for a Caucasian pediatric population. This study showed that, if hematology test results of the Ugandan children were assessed against “imported” international reference values, up to 44.6% of the study participants would have been excluded from clinical trials or would have been reported as adverse events in such trials. The present study was not only the first report of serum biochemistry reference ranges for children aged one to five years in Uganda but also one of very few such studies in Africa.

关键词： Normal Intervals Reference Uganda Children Hematology Serum Biochemistry Iganga

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An Interspecies Conserved Antigen of Plasmodium falciparum Containing Clusters of Asparagine Residues

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Wuhan University Journal of Natural Sciences 1998年第3期3卷 115-122页

作者： Ma Donghui Li Xiaojie Ma Huiwen fred A. S. kironde Wuhan University College of Life Sciences Wuhan China (GRID:grid.49470.3e) (ISNI:***) Shaheed Jeet Singh Marg International Center for Genetic Engineering and Biotechnology New Delhi India (GRID:grid.425195.e) (ISNI:0000 0004 0498 7682)

An interspecies conserved Plasmodium asparagine rich antigen, designated as ARK26, was isolated by immunoscreening *** genomic DNA expression library with mouse convalescent anti *** serum. Partial DNA sequence analysis reveals that ARK26 contains clusters of asparagines and no randomly repeated amino acid sequence motifs are observed. A 65 ×10 3 GST fusion protein is expressed by recombinant plasmid PGEX 5X 1(ARK26) in *** C strain ABLE K. Computer programs predict that two asparagine rich regions are among the possible antigenic epitopes of p37 encoded by ARK26. Interestingly, the sequence of ARK26 displays significant similarity to yeast and several other species' mitochondrial genes, and its possible function is discussed.

关键词： Plasmodium falciparum asparagine rich proteins,interspecies conserved antigens

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