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Algicidal activities of secondary metabolites of marine macroalgal-derived endophytic fungi

Journal of Oceanology and Limnology 2019年第1期37卷 112-121页

作者： MIAO fengping ZUO Jincheng LIU Xianghong JI Naiyun Yantai Institute of Coastal Zone Research Chinese Academy of Sciences College of Life Science Ludong University

Red tide occurrs frequently and causes signi?cant damage to the environment and human health. As a result, development of new effcient and environment friendly red-tide microalgae inhibitors has gained increasing attention in recent times. Algicolous endophytic fungi with unique habitats are promising sources for active agents owing to their abundant secondary metabolites and distinguished activities. In this study, the algicidal activities of 49 marine macroalgal-derived endophytic fungi against phytoplankton Alexandrium tamarense, Prorocentrum donghaiense, Heterosigma akashiwo, and Chattonella marina were examined using 96-well microplate. Four fungal strains, including Aspergillus wentii(pt-1), A. ustus(cf-42),and A. versicolor(dl-29, pt-20), exhibited potent algicidal activities. A total of 32 pure compounds isolated from these fungi were noted to possess dif ferent degrees of algicidal activities. Of those, 11 compounds comprising ?ve anthraquinones, two terpenoids, and four steroids showed high 24-h inhibition rates for the four red tide algae, with 24 h EC_(50) values ranging from 0.01 to 14.29 μg/mL. Among them, compound1(1,5-dihydroxy-3-methoxy-7-methylanthraquinone) presented the strongest activity against H. akashiwo,and could decrease its chlorophyll a(Chl a) and superoxide dismutase contents and increase the soluble protein, malondialdehyde, and peroxidase contents. These results suggested that the identi?ed anti-algal compound might inhibit the growth of red tide algae by weakening photosynthesis(reducing Chl a content),destroying cell membrane, and damaging the antioxidant system.

关键词： algicidal activity endophytic fungus secondary metabolite marine alga

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Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents

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Acta Oceanologica Sinica 2013年第6期32卷 75-81页

作者： LIU Lupeng WANG fengping XU Jun XIAO Xiang College of Marine Life Sciences Ocean University of China State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University School of System Biology Shanghai Jiao Tong University

Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrother- mal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the s- trains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30 MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyro- coccus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demon- strates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment.

关键词： hydrothermal vents Thermococcales Guaymas Basin

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Determination of carbon-fixing potential of Bathyarchaeota in marine sediment by DNA stable isotope probing analysis

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Science China Earth Sciences 2023年第4期66卷 910-917页

作者： Wenyue LIANG Tiantian YU Liang DONG Zhongjun JIA fengping WANG State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China School of Oceanography Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China

Bathyarchaeota is believed to play a crucial role in the global carbon cycle due to its vast biomass,broad distribution,and diverse ***,its physiological and metabolic features are hard to determine without pure *** metagenomic analyses have shown that Bathyarchaeota has a complete inorganic carbon fixation(Wood-Ljungdahl,WL)pathway,no direct functional confirmation has been *** explore the inorganic carbon fixation ability of Bathyarchaeota,we used lignin and sodium bicarbonate-^(13)C(NaH^(13)CO_(3))in the long-term incubation of marine sediment *** found that Bathyarchaeota grew continuously in the cultivation system with lignin,and its abundance increased up to 15.3 times after10 months,increasing its fraction of all archaea from 30%to 80%.We monitored theδ^(13)C of total organic carbon to identify microbial carbon fixation in the cultivation systems,finding that it increased in the first month while NaH^(13)CO_(3)was present but only increased continuously afterward when lignin was also ***,ultracentrifugation was performed on DNA extracted from samples at different cultivation stages to separate DNA of different buoyant densities,and bathyarchaeotal and bacterial 16S ribosomal RNA(r RNA)gene abundance were quantified using *** to bacteria,bathyarchaeotal 16S rRNA tended to be concentrated in heavy layers after 4 months of incubation with lignin and NaH^(13)CO_(3),indicating that Bathyarchaeota DNA contained^(13)C through proliferation based on lignin utilization and NaH^(13)CO_(3)assimilation,proving the carbon fixation capacity of Bathyarchaeota.

关键词： Bathyarchaeota Reductive acetyl-CoA pathway Carbon fixation DNA-SIP

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rhGM-CSF和纳米银联合外用对深Ⅱ度烫伤创面病原菌的影响

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中国医药导报 2014年第12期11卷 12-16页

作者：杨景哲王芳耿琪瑛陈凤平冯欣姝温海玲承德医学院附属医院烧伤整形科河北承德067000

目的研究重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和纳米银联合外用,对深Ⅱ度烫伤创面愈合过程病原菌分布及其耐药性分析。方法用Wistar大鼠建立深Ⅱ度烫伤模型,分为A、B、C、D四组,A组(30例):凡士林纱布覆盖,B组(30例):纳米银覆... 详细信息

目的研究重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和纳米银联合外用,对深Ⅱ度烫伤创面愈合过程病原菌分布及其耐药性分析。方法用Wistar大鼠建立深Ⅱ度烫伤模型,分为A、B、C、D四组,A组(30例):凡士林纱布覆盖,B组(30例):纳米银覆盖,C组(30例):rhGM-CSF涂抹创面,D组(30例):rhGM-CSF+纳米银覆盖,伤后第1、4、7、10、14、21天,创面分泌物细菌培养并计数,细菌分离鉴定,测定细菌耐药谱。结果 A组在第4、7、10、14、21天均培养出细菌[(3.22±1.04)×104/cm2、(4.57±1.15)×104/cm2、(8.13±1.25)×104/cm2、(16.36±3.22)×104/cm2、(14.52±3.10)×104/cm2];B、C两组在第10、14、21天培养出细菌[B组:(5.67±0.77)×104/cm2、(8.14±1.21)×104/cm2、(6.86±0.96)×104/cm2;C组:(4.38±1.44)×104/cm2、(6.80±0.85)×104/cm2、(4.94±0.40)×104/cm2];D组在第14、21天培养出细菌[(4.10±0.47)×104/cm2、(2.46±0.76)×104/cm2]。第10、14、21天,A、B、C、D组细菌计数比较,差异均有统计学意义(均PC组>B组>A组。结论 rhGM-CSF和纳米银联合外用,减少深Ⅱ度烫伤创面细菌生长,提高病原菌对抗菌药物的敏感性。

关键词：重组人粒细胞-巨噬细胞集落刺激因子纳米银烧伤创面病原菌耐药性

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Responses of marine-derived Trichoderma fungi to seawater and their potential antagonistic behaviour

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Journal of Oceanology and Limnology 2019年第2期37卷 525-534页

作者： SONG Yinping MIAO fengping LIU Xianghong JI Naiyun Yantai Institute of Coastal Zone Research Chinese Academy of Sciences Yantai 264003 China University of Chinese Academy of Sciences Beijing 100049 China

To explore the metabolic responses of marine-derived Trichoderma fungi to environmental stresses, the survivability, metabolism, and antagonism of ten marine isolates have been examined. Their survival in both freshwater and seawater indicates them to be facultative marine fungi, but they are more adaptable to marine environment. Most of them feature strain-specific and positive metabolic responses to seawater, which also usually result in the higher proportions of heteroatom-bearing and unsaturated units in mycelial constituents. Seawater factors can promote many strains to produce bioactive metabolites, including plant pathogen- and marine phytoplankton-inhibitory and marine animal-toxic ones, but the eff ects of NaCl are often weak or negative. The inhibition of marine phytoplankton corresponds to the intracellular accumulation of heteroatom-bearing and unsaturated units under seawater condition, and the varied toxicities to marine animals further signify the divergences of lipophilic exudates under diff erent conditions. The results may contribute to further understanding and mining the structural diversity and biological activity of secondary metabolites from marine-derived Trichoderma fungi.

关键词： Trichoderma fungus metabolic response seawater antagonistic potential

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Core Metabolic Features and Hot Origin of Bathyarchaeota

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Engineering 2019年第3期5卷 498-504页

作者： Xiaoyuan Feng Yinzhao Wang Rahul Zubin fengping Wang State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Shanghai 200240 China

The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain largely unknown. In this article, a comparative metabolic analysis of 15 newly reconstructed and 36 published metagenomic assembled genomes (MAGs) spanning 10 subgroups was performed, revealing the core metabolic features of Bathyarchaeota—namely, protein, lipid, and benzoate degradation;glycolysis;and the Wood–Ljungdahl (WL) pathway, indicating an acetyl-CoA-centralized metabolism within this phylum. Furthermore, a partial tricarboxylic acid (TCA) cycle, acetogenesis, and sulfur-related metabolic pathways were found in specific subgroups, suggesting versatile metabolic capabilities and ecological functions of different subgroups. Intriguingly, most of the MAGs from the Bathy-21 and -22 subgroups, which are placed at the phylogenetic root of all bathyarchaeotal lineages and likely represent the ancient Bathyarchaeota types, were found in hydrothermal environments and encoded reverse gyrase, suggesting a hyperthermophilic feature. This work reveals the core metabolic features of Bathyarchaeota, and indicates a hot origin of this archaeal phylum.

关键词： Bathyarchaeota Metagenomics Comparative genomics Hyperthermophilic adaptation

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Three nitrogen-containing metabolites from an algicolous isolate of Trichoderma asperellum

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Marine Life Science & Technology 2020年第2期2卷 155-160页

作者： Yinping Song fengping Miao Xiuli Yin Naiyun Ji Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China

As one of the most productive species,Trichoderma asperellum,especially isolated from marine algae,has contributed a number of new terpenes and nitrogen-bearing compounds,encouraging our continuous efforts to further explore ***,three new nitrogen-containing secondary metabolites,including cyclo(L-5-MeO-Pro-L-5-MeO-Pro)(1),5'-acetoxy-deoxycyclonerin B(2),and 5'-acetoxy-deoxycyclonerin D(3)were isolated from the marine alga-derived endophytic fungus Trichoderma asperellum *** structures and relative configurations were fully elucidated through spectroscopic techniques,mainly including 1D/2D NMR and *** absolute configurations of 1 and 2 were ascertained on the basis of X-ray diffraction(Cu Kα radiation)and ECD data,*** isolation of these new nitrogen-bearing compounds adds to the structural diversity of marine algicolous Trichoderma spp.,and they display potent inhibition against some marine phytoplankton species.

关键词： Trichoderma Diketopiperazine Sesquiterpene Cyclonerane

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Discovering the roles of subsurface microorganisms: Progress and future of deep biosphere investigation

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Chinese Science Bulletin 2013年第4期58卷 456-467页

作者： WANG fengping LU ShuLin ORCUTT Beth N XIE Wei chen Ying XIAO Xiang EDWARDS Katrina J State Key Laboratory of Microbial Metabolism and State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Key Laboratory of Systems Biomedicine Ministry of Education Shanghai Jiao Tong University Center for Geomicrobiology Aarhus University Bigelow Laboratory for Ocean Sciences 60 Bigelow Drive School of Ocean and Earth Sciences Tongji University The University of Southern California Departments of Biological & Earth Sciences

The discovery of the marine "deep biosphere"-microorganisms living deep below the seafloor-is one of the most significant and exciting discoveries since the ocean drilling program began more than 40 years ago. Study of the deep biosphere has become a research frontier and a hot spot both for geological and biological sciences. Here, we introduce the history of the discovery of the deep biosphere, and then we describe the types of environments for life below the seafloor, the energy sources for the living creatures, the diversity of organisms within the deep biosphere, and the new tools and technologies used in this research field. We will highlight several recently completed Integrated Ocean Drilling Program Expeditions, which targeted the subseafloor deep biosphere within the crust and sediments. Finally, future research directions and challenges of deep biosphere investigation towards uncovering the roles of subsurface microorganisms will be briefly addressed.

关键词：深部生物圈地下微生物综合大洋钻探计划深海底生物科学多样性沉积物海洋

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Super-resolution of Solar Magnetograms Using Deep Learning

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Research in Astronomy and Astrophysics 2022年第8期22卷 218-229页

作者： fengping Dou Long Xu Zhixiang Ren Dong Zhao Xinze Zhang State Key Laboratory of Space Weather National Space Science CenterChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Peng cheng National Laboratory Shenzhen 518000China State Key Laboratory of Virtual Reality Technology and Systems School of Computer Science and EngineeringBeihang UniversityBeijing 100191China

Currently,data-driven models of solar activity forecast are investigated extensively by using machine *** model training,it is highly demanded to establish a large database which may contain observations coming from different instruments with different spatio-temporal *** this paper,we employ deep learning models for super-resolution(SR)of magnetogram of Michelson Doppler Imager(MDI)in order to achieve the same spatial resolution of Helioseismic and Magnetic Imager(HMI).First,a generative adversarial network(GAN)is designed to transfer characteristics of MDI onto downscaled HMI,getting low-resolution HMI magnetogram in the same domain as ***,with the paired low-resolution and high-resolution HMI magnetograms,another GAN is trained in a supervised learning way,which consists of two streams,one is for generating high-fidelity image content,the other is explicitly optimized for generating elaborate image ***,these two streams work together to guarantee both high-fidelity and photorealistic super-resolved *** results demonstrate that the proposed method can generate super-resolved magnetograms with perceptual-pleasant visual ***,the best PSNR,LPIPS,RMSE,comparable SSIM and CC are obtained by the proposed *** source code and data set can be accessed via https://***/filterbank/SPSR.

关键词： techniques:image processing Sun:magnetic fields Sun:atmosphere

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超前视听分散干预在PPH术中的应用研究

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河北医学 2022年第5期28卷 843-847页

作者：刘晓丽陈凤平高英杰贾国璞司景元刘家红富煜赵红莲承德医学院附属医院河北承德067000 河北省滦平县医院河北滦平067000 河北省承德市食品药品检验检测中心河北承德067000

目的:研究超前视听分散干预痔上黏膜环切术(PPH)中血管迷走神经反射(VVR)的临床作用。方法:将本院2017年9月至2019年9月收集到的100例进行PPH的患者采取简单随机分组方式分为两组:试验组50例,对照组50例。试验组采用超前视听分散术干预... 详细信息

目的:研究超前视听分散干预痔上黏膜环切术(PPH)中血管迷走神经反射(VVR)的临床作用。方法:将本院2017年9月至2019年9月收集到的100例进行PPH的患者采取简单随机分组方式分为两组:试验组50例,对照组50例。试验组采用超前视听分散术干预,各组术中均给予阿托品和视听分散预防术中血管迷走神经反射发生。对比试验组和对照组预防术中发生血管迷走神经反射的数据。结果:试验组2%(1/50)发生VVR,低于对照组16%(8/50),比较结果显示差异有统计学意义(P0.05)。PPH击发时对照组MAP、HR水平较进术前明显下降(P0.05)。结论:超前视听分散技术能减少PPH患者围手术期的VVR。

关键词：超前视听分散骶麻痔上黏膜环切术血管迷走神经反射

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