Determination of carbon-fixing potential of Bathyarchaeota in marine sediment by DNA stable isotope probing analysis

作     者：Wenyue LIANG Tiantian YU Liang DONG Zhongjun JIA Fengping WANG 

作者机构：State Key Laboratory of Microbial MetabolismSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China School of OceanographyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Soil and Sustainable AgricultureInstitute of Soil ScienceChinese Academy of SciencesNanjing 210008China 

出 版 物：《Science China Earth Sciences》 (中国科学（地球科学英文版）)

年 卷 期：2023年第66卷第4期

页      面：910-917页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0708[理学-地球物理学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the State Key R&D Project of China(Grant No.2016YFA0601102) the National Natural Science Foundation of China(Grant Nos.91751205,41525011&41867057) 

主　　题：Bathyarchaeota Reductive acetyl-CoA pathway Carbon fixation DNA-SIP 

摘      要：Bathyarchaeota is believed to play a crucial role in the global carbon cycle due to its vast biomass,broad distribution,and diverse ***,its physiological and metabolic features are hard to determine without pure *** metagenomic analyses have shown that Bathyarchaeota has a complete inorganic carbon fixation(Wood-Ljungdahl,WL)pathway,no direct functional confirmation has been *** explore the inorganic carbon fixation ability of Bathyarchaeota,we used lignin and sodium bicarbonate-^(13)C(NaH^(13)CO_(3))in the long-term incubation of marine sediment *** found that Bathyarchaeota grew continuously in the cultivation system with lignin,and its abundance increased up to 15.3 times after10 months,increasing its fraction of all archaea from 30%to 80%.We monitored theδ^(13)C of total organic carbon to identify microbial carbon fixation in the cultivation systems,finding that it increased in the first month while NaH^(13)CO_(3)was present but only increased continuously afterward when lignin was also ***,ultracentrifugation was performed on DNA extracted from samples at different cultivation stages to separate DNA of different buoyant densities,and bathyarchaeotal and bacterial 16S ribosomal RNA(r RNA)gene abundance were quantified using *** to bacteria,bathyarchaeotal 16S rRNA tended to be concentrated in heavy layers after 4 months of incubation with lignin and NaH^(13)CO_(3),indicating that Bathyarchaeota DNA contained^(13)C through proliferation based on lignin utilization and NaH^(13)CO_(3)assimilation,proving the carbon fixation capacity of Bathyarchaeota.