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Efficacy of chitosan and sodium alginate scaffolds for repair of spinal cord injury in rats

Neural Regeneration Research 2018年第3期13卷 502-509页

作者： Zi-ang Yao feng-jia Chen Hong-li Cui Tong Lin Na Guo Hai-ge Wu School of Life Science and Technology Dalian University

Spinal cord injury results in the loss of motor and sensory pathways and spontaneous regeneration of adult mammalian spinal cord neurons is limited. Chitosan and sodium alginate have good biocompatibility, biodegradability, and are suitable to assist the recovery of damaged tissues, such as skin, bone and nerve. Chitosan scaffolds, sodium alginate scaffolds and chitosan-sodium alginate scaffolds were separately transplanted into rats with spinal cord hemisection. Basso-Beattie-Bresnahan locomotor rating scale scores and electrophysiological results showed that chitosan scaffolds promoted recovery of locomotor capacity and nerve transduction of the experimental *** days after surgery, chitosan scaffolds retained the original shape of the spinal cord. Compared with sodium alginate scaffolds- and chitosan-sodium alginate scaffolds-transplanted rats, more neurofilament-H-immunoreactive cells （regenerating nerve fibers） and less glial fibrillary acidic protein-immunoreactive cells （astrocytic scar tissue） were observed at the injury site of experimental rats in chitosan scaffold-transplanted rats. Due to the fast degradation rate of sodium alginate, sodium alginate scaffolds and composite material scaffolds did not have a supporting and bridging effect on the damaged tissue. Above all, compared with sodium alginate and composite material scaffolds, chitosan had better biocompatibility, could promote the regeneration of nerve fibers and prevent the formation of scar tissue,and as such, is more suitable to help the repair of spinal cord injury.

关键词： nerve regeneration spinal cord injury chitosan sodium alginate functional recovery scaffold neurofilament-H glial fibrillary acidic protein scar tissue locomotor capacity neural regeneration

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A Two-Layer Real-Time Rate Control Algorithm Based on Lagrange Optimum Algorithm

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Wuhan University Journal of Natural Sciences 2009年第1期14卷 35-38页

作者： HU feng LIU jia CHEN Shuzhen School of Electronic Information Wuhan University Wuhan 430079 Hubei China

Because of the iterative process in the rate control algorithm of the H.264 draft, it is not suitable for real-time transmission. A two-layer real-time rate control algorithm based on the Lagrange optimum algorithm is proposed. This algorithm consists of two layers, namely, the group of pictures （GOP） layer and the frame layer. The simulation results show that the proposed algorithm is competitive in terms of peak signal-to-noise ratio （PSNR）, real bitrates and the Q-value among frames over the original algo- rithm.

关键词： H.264 Lagrange optimum peak signal to noise ratio(PSNR) quantize rate-distortion

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Novel Bis-β-diketone-type Ligand and Its Copper and Zinc Complexes for Two-photon Biological Imaging

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Chemical Research in Chinese Universities 2012年第4期28卷 647-651页

作者： ZHOU Shuang-sheng XUE Xuan WEI Dong jiaNG Bo WANG jia-feng LU Cheng-hua Anhui Engineering Research Center of Modernized Chinese Medicine Department of PharmacyAnhui University of Traditional Chinese MedicineHefei 230031P.R.China

A curcumin derivative ligand,1,7-bis（3-methoxyl-4-oxyethylacetate）phenyl-1,6-heptadiene-3,5-diketone（diethyl acetatecurcumin,abbreviated as HL）,and its Cu（II） and Zn（II） complexes have been synthesized and characterized by elemental analyses,infrared（IR）,1H NMR and molar *** experimental results show that the resulting complexes bear strong two-photon excited fluorescence（TPEF） in N,N-dimethyformamide solvent,which has been proven to be potentially useful for two-photon microscopy imaging in living *** addition,cytotoxicity tests show that the low-micromolar concentrations of metal-ligand complex（ML2） did not cause significant reduction in cell viability over a period of,at least,24 h and should be safe for further biological studies.

关键词： Diethyl acetatecurcumin Photophysical property Cell imaging

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Synthesis and assembly of full-length cyanophage A-4L genome

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Synthetic and Systems Biotechnology 2023年第1期8卷 121-128页

作者： Ting Zhang Bonan Xu jia feng Pingbo Ge Guorui Li jiabao Zhang jianting Zhou jianlan jiang School of Chemical Engineering and Technology Tianjin UniversityTianjin300072China Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin300072China Frontier Science Center for Synthetic Biology(Ministry of Education) Tianjin UniversityTianjin300072China

Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial ***,no synthetic cyanophage genome has been constructed and where its obstacles are ***,we survey a stretch of 16 kb length sequence of cyanophage A-4L that is unclonable in Escherichia *** test 12 predicted promoters of cyanophage A-4L which were verified all active in ***,we screen for eight ORFs that hindered the assembly of intermediate DNA fragments in *** and describe that seven ORFs in the 16 kb sequence could not be separately cloned in *** of unclonable ORFs in high-copy-number plasmid were successfully cloned using low-copy-number vector,suggesting that these ORFs were *** propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ***,we de novo synthesized and assembled the full-length genome of cyanophage *** work deepens the understanding of synthetic cyanophages studies.

关键词： Genome assembly Cyanophage A-4L Toxic ORFs screening

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Assembling structurally customizable synthetic carriers of si RNA through thermodynamically self-regulated process

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Asian Journal of Pharmaceutical Sciences 2020年第3期15卷 356-364页

作者： Guang Bai Tong Xue Xiaotao Dong Uday Kumar Chinta jia feng Tuo Jin Fei Wu School of Pharmacy Shanghai Jiao Tong UniversityShanghai 200240China

This study demonstrates that our previously reported polywraplex, a synthetic siRNA carrier consisting of a uni-molecular polyplex core of customizable size and a self-assembled triblock copolymer envelop, may be constructed using dendrimers as the crosslinking junctions. Replacing the branched low molecular weight PEI with polyamidoamine(PAMAM) dendrimer in the zeta potential regulated polymerization resulted in the similar network structured cationic polymer with electron microscopically visible crosslinking junctions. This visibility may offer a convenient way to characterize the molecular structure of the rationally designed networked siRNA-packing cationic polymer without altering its chemical properties and biologic functions. A series of physical-chemical characterizations and biological assays, comprising size, zeta potential, pre-phagocytic siRNA leaking and degradation, and silencing of functional genes, confirmed that the advanced properties of polywraplexes remained with the dendrimer junctions. Although sixth generation PAMAM dendrimer was used as the crosslinking junctions in the size-customizable polymerization for electron microscopic observation, lower generation dendrimer should also work in case more practical and structurally defined cationic polymer is needed.

关键词： Networked cationic polymer Thermodynamically self-regulated processes siRNA delivery Unimolecular polyplex Zeta potential regulated polymerization

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Culture and identification of neonatal rat brain-derived neural stem cells

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World Journal of Stem Cells 2023年第6期15卷 607-615页

作者： Qing-Zhong Zhou Xiao-Lan feng Xu-feng jia Nurul Huda Binti Mohd Nor Mohd Hezery Bin Harun Da-Xiong feng Wan Aliaa Wan Sulaiman Department of Orthopedics The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Neurology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia Department of Radiology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Orthopedics The Peoples’Hospital of Jianyang CityJianyang 641400Sichuan ProvinceChina Department of Human Anatomi Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang Selangor43400Malaysia Department of Orthopedics Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia

BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)*** to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively *** To establish a simplified and efficient method for culture and identification of neonatal rat brain-derived *** First,curved tip operating scissors were used to dissect brain tissues from new born rats(2 to 3 d)and the brain tissues were cut into approximately 1 mm^(3)*** the single cell suspension through a nylon mesh(200-mesh)and culture the sections in *** was conducted with TrypLTM Express combined with mechanical tapping and pipetting ***,identify the 5th generation of passaged NSCs as well as the revived NSCs from *** incorporation method was used to detect self-renew and proliferation capabilities of *** NSCs specific antibodies(anti-nestin,NF200,NSE and GFAP antibodies)were used to identify NSCs specific surface markers and muti-differentiation capabilities by immunofluorescence *** Brain derived cells from newborn rats(2 to 3 d)proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable *** BrdU was incorporated into the 5th generation of passaged cells,positive BrdU cells and nestin cells were observed by immunofluorescence *** induction of dissociation using 5%fetal bovine serum,positive NF200,NSE and GFAP cells were observed by immunofluorescence *** This is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification.

关键词： Neonatal rats Brain-derived neural stem cells Culture Identification

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Navigating the complex landscape of crawling-type gastric adenocarcinomas:Insights and implications for clinical practice

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World Journal of Gastrointestinal Oncology 2024年第11期16卷 4309-4314页

作者： Hai-Bo Yu Ke-feng jia Xing-Fen Wang Bao-Yu Li Qi Xin Laboratory The Second Hospital of Tianjin Medical UniversityTianjin 300211China Department of Radiology Tianjin Third Central HospitalTianjin 300170China Department of Pathology The Second Hospital of Tianjin Medical UniversityTianjin 300211China Department of Gastrointestinal Surgery The Second Hospital of Tianjin Medical UniversityTianjin 300211China Department of Pathology The Third Central Hospital of TianjinTianjin 300170China

In this editorial,we comment on an article by Xu et *** article describes a case of crawling-type gastric adenocarcinoma(CRA)distinguished by its rare occurrence and diagnostic *** reviewed the detailed case-report findings showcasing clinical,pathological,and molecular characteristics of CRA that shed light on its elusive nature and challenges for early detection and *** case underscored the significance of advanced diagnostic tools such as endoscopic submucosal *** was placed on the molecular peculiarities of CRA,including the higher mutation rates of genes such as TP53 and RHOA and the notable absence of HER2 amplification,differentiating it from more conventional forms of gastric *** this editorial,we advocate for a multidisciplinary approach to effectively manage this rare subtype and highlight the necessity for precision in both diagnostic and therapeutic ***,a heightened awareness urging the adoption of advanced diagnostic techniques and collaborative approaches is necessary among clinicians and *** aim to contribute to the ongoing discourse in gastrointestinal oncology,emphasizing the importance of recognizing and addressing the complexities associated with rare cancer subtypes such as CRA.

关键词： Crawling-type gastric adenocarcinoma Diagnosis Pathology Endoscopy Treatment

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Regional public culture related laws and the development of public library undertaking in China

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Journal of Library Science in China 2014年 213-230页

作者： jia feng Institute of Literature Shanghai Academy of Social Science

The content of regional public culture related laws have gradually deepened and expanded since the first public cultural related law was *** content covers the construction of facilities,service supply,funding and talent team construction,management system,social forces to participate in the development and so on,and there are many breakthroughs in the *** order to gradually carry out the legal and systematic development of public libraries,some breakthroughs in the future should be concentrated on:making policy of the interaction between urban and rural areas for the public library’s central-branch system,establishing demand investigation and survey policies and increasing the intensity of resource distribution,paying attention to the continuous training of all kinds of library practitioners and practicing the policy of card mount guard,setting up special funds and the corresponding system for public library development,improving the performance evaluation system to make all the indicators lawfully enforceable.

关键词： Public library Public culture Regional laws

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Clinical Characteristics of Bloodstream Infections in Pediatric Acute Leukemia： A Single-center Experience with 231 Patients

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Chinese Medical Journal 2017年第17期130卷 2076-2081页

作者： jia-feng Yao Nan Li Jin jiang Hematology Oncology Center Beijing Key Laboratory of Pediatric Hematology OncologyNational Key Discipline of PediatricsMinistry of EducationBeijing Children's HospitalCapital Medical UniversityBeijing 100045China

Background：Acute leukemia is the most common pediatric hematological *** infections （BSIs） are severe complications in these patients during *** study aimed to explore the clinical presentation and etiology of BSI,as well as the common sites of infection,and to provide a basis for the rational regarding antibiotic ***：We performed a retrospective chart review of all pediatric patients who had acute leukemia accompanied by a BSI in our hospital from December 2011 to September *** patients were selected based on clinical presentation and had to have at least one positive blood culture for *** basic clinical characteristics,blood culture results,and antimicrobial susceptibilities were ***：All 231 patients had a fever;of them,12 patients continued to have a ***-five patients had nonremitting （NR） leukemia,and 206 patients achieved complete remission （CR）.Differences in the duration of fever between the NR and CR groups were significant （9.6 ＆#177; 7.9 vs.5.1 ＆#177; 3.8 days,P =0.016）.One hundred and eighty patients had *** in fever duration between the agranulocytosis and nonagranulocytosis groups were significant （6.2 ＆#177; 5.1 vs.4.1 ＆#177; 2.6 days,P =0.001）.The other sites of infection in these 231 patients were the lung,mouth,digestive tract,and *** culture comprised 2635 *** were 619 samples,which were *** the 619 positive blood culture samples,59.9% had Gram-negative bacteria,39.3% had Gram-positive bacteria,and 0.8% had *** primary pathogens were Pseudomonas aeruginosa,Enterobactercloacae,Escherichia coli,and Klebsiella *** these 231 patients,217 patients were *** effective treatment ratio was 94%.Conclusions：Gram-negative bacteria were the main pathogenic bacteria in patients with acute leukemia in our *** primary illness,agranulocytosis,and drug-resistant pathogenic bacteria were all risk factors for poo

关键词： Acute Leukemia Agranulocytosis Bloodstream Infection Etiology Pediatric

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Analysis of Transition Mechanism of Cubic Boron Nitride Single Crystals under High Pressure-High Temperature with Valence Electron Structure Calculation

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Chinese Physics Letters 2019年第1期36卷 10-13页

作者： Mei-Zhe Lv Bin Xu Li-Chao Cai feng jia Xing-Dong Yuan School of Materials Science and Engineering Shandong University School of Materials Science and Engineering Shandong Jianzhu University

The possibilities of hexagonal boron nitride(hBN) and lithium boron nitride(Li_3BN_2) transition into cubic boron nitride(cBN) under synthetic pressure 5.0 GPa and synthetic temperature 1700 K are analyzed with the use of the empirical electron theory of solids and molecules. The relative differences in electron density are calculated for dozens of bi-phase interfaces hBN/cBN, Li_3BN_2/cBN. These relative differences of hBN/cBN are in good agreement with the first order of approximation(<10%), while those of Li_3BN_2/cBN are much greater than 10%.This analysis suggests that Li_3BN_2 is impossible to be intermediate phase but is a catalyst and cBN should be directly transformed by hBN.

关键词： Transition Mechanism Cubic Boron Valence Electron Structure Calculation

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