Synthesis and assembly of full-length cyanophage A-4L genome

作     者：Ting Zhang Bonan Xu Jia Feng Pingbo Ge Guorui Li Jiabao Zhang Jianting Zhou Jianlan Jiang 

作者机构：School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Key Laboratory of Systems Bioengineering(Ministry of Education)Tianjin UniversityTianjin300072China Frontier Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin300072China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2023年第8卷第1期

页      面：121-128页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the grants from the National Key Research and Development Program of China (No.2018YFA0903000) the National Natural Science Foundation of China (31901019)and the China Postdoctoral Science foundation (2021M692389) 

主　　题：Genome assembly Cyanophage A-4L Toxic ORFs screening 

摘      要：Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial ***,no synthetic cyanophage genome has been constructed and where its obstacles are ***,we survey a stretch of 16 kb length sequence of cyanophage A-4L that is unclonable in Escherichia *** test 12 predicted promoters of cyanophage A-4L which were verified all active in ***,we screen for eight ORFs that hindered the assembly of intermediate DNA fragments in *** and describe that seven ORFs in the 16 kb sequence could not be separately cloned in *** of unclonable ORFs in high-copy-number plasmid were successfully cloned using low-copy-number vector,suggesting that these ORFs were *** propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ***,we de novo synthesized and assembled the full-length genome of cyanophage *** work deepens the understanding of synthetic cyanophages studies.