检索结果-南通市图书馆

A new oxidative pathway of nitric oxide production from oximes in plants

molecular Plant 2024年第1期17卷 178-198页

作者： Pedro Lopez-Gomez j.vier Buezo marina Urra Alfonso Cornej. Raquel Esteban j.rge fernandez de los Reyes Estibaliz Urarte estefania Rodriguez-Dobreva Alej.ndro Chamizo-Ampudia Alej.ndro Eguaras Sebastian Wolf Daniel marino Victor martinez-merino j.se F.moran Institute for multidisciplinary Research in Applied Biology(ImAB) Department of SciencesPublic University of Navarre(UPNA)Avda.de Pamplona 12331192 MutilvaSpain Institute for Advanced materials and mathematics(InAmAT2) Department of SciencesPublic University of Navarre(UPNA)Campus de Arosadia31006 PamplonaSpain Department of Plant Biology and Ecology University of the Basque Country(UPV/EHU)Sarriena s/nApdo.64448080 BilbaoSpain Center for Plant molecular Biology(ZmBP) University of TubingenGeschwister-Scholl-Platz72074 TubingenGermany Institute of natural Resources and Agrobiology of Salamanca(IRnASA) Department of Plant-Microorganism InteractionsSpanish National Research Council(CSIC)Cordel de Merinas40-5237008SalamancaSpain Department of Biochemistry Area of Molecular BiologyVeterinary FacultyUniversity of LeonCampus de Vegazanas/n.24071 LeonSpain

nitric oxide(nO)is an essential reactive oxygen species and a signal molecule in *** several studies have proposed the occurrence of oxidative nO production,only reductive routes for nO production,such as the nitrate(nO_(3)^(-))-upper-reductase pathway,have been evidenced to date in land ***,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and nO3−,evidencing the existence of a metabolic pathway for oxidative production of *** hypothesized that oximes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in nO *** detected the production of nO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and *** stimulated the reaction,while superoxide dismutase inhibited ***,mouse nO synthase can also use IAOx to produce nO at a lower rate than *** provided a full mechanism for POD-dependent nO production from IAOx consistent with the experimental data and supported by density functional theory *** showed that the addition of IAOx to extracts from medicago truncatula increased the in vitro production of nO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for nO donors,and a more than 10-fold increase in IAOx dehydratase ***,we found that in vivo supplementation of IAOx increased nO production in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced *** data show that the release of nO by IAOx,as well as its auxinic effect,explain the superroot ***,our study reveals that plants produce nO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus introducing a long-awaited oxidative pathway to nO production in plants.T

关键词： ammonium oxidation auxin nitric oxide oxime peroxidase root phenotype

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Commitment of human mesenchymal stromal cells to skeletal lineages is independent of their morphogenetic capacity

引用

World j.urnal of Stem Cells 2023年第7期15卷 701-712页

作者： j.ssica Cristina marín-Llera Damián García-García estefania Garay-Pacheco Victor Adrian Cortes-morales j.an j.se montesinos-montesinos j.sus Chimal-monroy medicina Genómica y Toxicología Ambiental Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico Laboratorio de Células Troncales mesenquimales Unidad de Investigación Médica en Enfermedades OncológicasHospital de Oncología Centro Medico Nacional Siglo XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Departamento de medicina Genómica y Toxicología Ambiental Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico

BACKGROUnD mesenchymal stromal cells(mSCs)are multipotent cell populations obtained from fetal and adult *** share some characteristics with limb bud mesodermal cells such as differentiation potential into osteogenic,chondrogenic,and tenogenic lineages and an embryonic mesodermal *** mSCs differentiate into skeletal-related lineages in vitro,they have not been shown to selforganize into complex skeletal structures or connective tissues,as in the *** this work,we demonstrate that the expression of molecular markers to commit mSCs to skeletal lineages is not sufficient to generate skeletal elements in *** To evaluate the potential of mSCs to differentiate into skeletal lineages and generate complex skeletal structures using the recombinant limb(RL)*** We used the experimental system of RLs from dissociated-reaggregated human placenta(PL)and umbilical cord blood(UCB)*** being harvested and reaggregated in a pellet,cultured cells were introduced into an ectodermal cover obtained from an early chicken limb ***,this filled ectoderm was grafted into the back of a donor chick *** these conditions,the cells received and responded to the ectoderm’s embryonic signals in a spatiotemporal manner to differentiate and pattern into skeletal *** response to differentiation and morphogenetic signals was evaluated by quantitative poly-merase chain reaction,histology,immunofluorescence,scanning electron microscopy,and in situ *** We found that human PL-mSCs and UCB-mSCs constituting the RLs expressed chondrogenic,osteogenic,and tenogenic molecular markers while differentially committing into limb lineages but could not generate complex structures in ***-RL from PL or UCB were committed early to chondrogenic ***,the UCB-RL osteogenic commitment was favored,although preferentially to a tenogenic cell *** findings suggest that the commitment of mSCs to differentiate int

关键词： Human mesenchymal stromal cells Recombinant limbs mesenchymal stromal cell morphogene-sis mesenchymal stromal cell in vivo differentiation Skeletal tissues

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of mitochondrial function and endoplasmic reticulum stress by nitric oxide in pluripotent stem cells

引用

World j.urnal of Stem Cells 2017年第2期9卷 26-36页

作者： estefania Caballano-Infantes j.sé Terron-Bautista Amparo Beltrán-Povea Gladys m Cahuana Bernat Soria Haj.i nabil Francisco j.Bedoya j.an R Tej.do Andalusian Center for molecular Biology and Regenerative medicine-University Pablo de Olavide 41092 SevilleSpain Biomedical Research network on Diabetes and Related metabolic Diseases(CIBERDEm) Instituto de Salud CarlosⅢ28029 MadridSpain Andalusian Center for molecular Biology and Regenerative medicine(CABImER) 41092 SevilleSpain Andalusian Center for molecular Biology and Regenerative medicine Fundación Progreso y Salud41092 SevilleSpain Centre for Pharmacology and Therapeutics Division of Experimental MedicineImperial College LondonHammersmith Hospital CampusLondon W12 0NNUnited Kingdom

mitochondrial dysfunction and endoplasmic reticulum stress(ERS) are global processes that are interrelated and regulated by several stress factors. nitric oxide(nO) is a multifunctional biomolecule with many varieties of physiological and pathological functions, such as the regulation of cytochrome c inhibition and activation of the immune response, ERS and DnA damage; these actions are dose-dependent. It has been reported that in embryonic stem cells, nO has a dual role, controlling differentiation, survival and pluripotency, but the molecular mechanisms by which it modulates these functions are not yet known. Low levels of nO maintain pluripotency and induce mitochondrial biogenesis. It is well established that nO disrupts the mitochondrial respiratory chain and causes changes in mitochondrial Ca^(2+) flux that induce ERS. Thus, at high concentrations, nO becomes a potential differentiation agent due to the relationship between ERS and the unfolded protein response in many differentiated cell lines. nevertheless, many studies have demonstratedthe need for physiological levels of nO for a proper ERS response. In this review, we stress the importance of the relationships between nO levels, ERS and mitochondrial dysfunction that control stem cell fate as a new approach to possible cell therapy strategies.

关键词： Endoplasmic reticulum stress mitochondrial function nitric oxide Pluripotency Cell differentiation mitochondrial biogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

neurological manifestations with j.gular vein thrombosis linked to an inflammatory profile may be a sequela of long COVID

引用

neuroprotection 2023年第2期1卷 170-175页

作者： Elizabeth mendoza-Portillo estefania Aleman-navarro G.Aleph Prieto Yvonne Rosenstein j.se j.Lozano-nuevo Araceli Perez-Lopez Instituto nacional de neurología y neurocirugía manuel Velasco Suarez Ciudad de MéxicoMéxico Instituto de Biotecnología Universidad Nacional Autónoma de México(UNAM)CuernavacaMorelosMéxico Instituto de neurobiología UNAM-JuriquillaQuerétaroMéxico Institute for memory Impairments and neurological Disorders University of California-IrvineIrvineCaliforniaUSA Hospital General de Ticomán Secretaría de Salud de la Ciudad de MéxicoCiudad de MéxicoMéxico Unidad de Investigación en Biomedicina Facultad de Estudios Superiores IztacalaUNAMTlalneplantlaEstado de MéxicoMéxico

more than 670 million cases of coronavirus disease 2019(COVID-19)have been recorded worldwide in the 3 years since the start of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)*** 45%of survivors of COVID-19 develop a syndrome known as long-term COVID,in which symptoms persist even months after the acute *** 76%of patients with long COVID experience neurological ***,patients who have survived COVID-19 have an increased risk of cerebral venous *** case report describes a 41-year-old woman who developed neurological manifestations associated with j.gular vein thrombosis 24 h after administration of the Oxford–AstraZeneca(ChAdOx1 nCoV-19)vaccine(AstraZeneca-Serum Institute of India).She had been infected with SARS-CoV-2 three months before *** initially suspected to be a case of vaccine-induced immune thrombotic thrombocytopenia(VITT)in view of her recent vaccination,the patient did not have any hallmarks of VITT,such as thrombocytopenia,an increased D-dimer level,or antibodies against platelet ***,the neurological manifestations were associated with a high concentration of inflammatory cytokines,including interleukin(IL)-6,IL-17A,and IL-21,and elevated neutrophil levels in cerebrospinal fluid,suggesting that inflammatory immune components had a role in the development of thrombotic events and pointing to an alternative *** this case,the laboratory results indicated that the neurological manifestations associated with j.gular vein thrombosis were not associated with ***,we propose that the thrombosis of the left j.gular vein was a sequela of SARS-CoV-2 infection.

关键词： cerebral venous thrombosis long COVID vaccine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of nitric oxide in the maintenance of pluripotency and regulation of the hypoxia response in stem cells

引用

World j.urnal of Stem Cells 2015年第3期7卷 605-617页

作者： Amparo Beltran-Povea estefania Caballano-Infantes Carmen Salguero-Aranda Franz martín Bernat Soria Francisco j.Bedoya j.an R Tej.do Gladys m Cahuana Andalusian Center for molecular Biology and Regenerative medicine-University Pablo de Olavide CIBERDEM RED-TERCEL 41092 Seville Spain Andalusian Center for molecular Biology and Regenerative medicine (CABImER) -Fundación Progreso y Salud CIBERDEM RED-TERCEL 41092 Seville Spain

Stem cell pluripotency and differentiation are global processes regulated by several pathways that have been studied intensively over recent years. nitric oxide(nO) is an important molecule that affects gene expression at the level of transcription and translation and regulates cell survival and proliferation in diverse cell types. In embryonic stem cells nO has a dual role, controlling differentiation and survival, but the molecular mechanisms by which it modulates these functions are not completely defined. nO is a physiological regulator of cell respiration through the inhibition of cytochrome c oxidase. many researchers have been examining the role that nO plays in other aspects of metabolism such as the cellular bioenergetics state, the hypoxia response and the relationship of these areas to stem cell stemness.

关键词： Pluripotency Differentiation nitric oxide metabolism Hypoxia Stem cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A solution scan of societal options to reduce transmission and spread of respiratory viruses:SARS-CoV-2 as a case study

引用

j.urnal of Biosafety and Biosecurity 2021年第2期3卷 84-90页

作者： William j.Sutherland nigel G.Taylor David C.Aldridge Philip martin Catherine Rhodes gorm Shackelford Simon Beard Haydn Belfield Andrew j.Bladon Cameron Brick Alec P.Christie Andrew P.Dobson Harriet Downey Amelia S.C.Hood Fangyuan Hua Alice C.Hughes Rebecca m.j.rvis Douglas macFarlane William H.morgan Anne-Christinemupepele Stefan j.marciniak Cassidy nelson SeánÓhÉigeartaigh Clarissa Rios Roj.s Katherine A.Sainsbury Rebecca K.Smith Lalitha S.Sundaram Ann Thornton j.hn Watkins Thomas B.White Kate Willott Silviu O.Petrovan BioRISC(Biosecurity Research Initiative at St Catharine’s) St Catharine’s CollegeCambridge CB21RLUK Department of Zoology University of CambridgeThe David Attenborough BuildingPembroke StreetCambridge CB23QZUK Tour du Valat Research Institute for the Conservation of Mediterranean Wetlands13200 ArlesFrance Centre for the Study of Existential Risk University of CambridgeCB21SBUK Department of Social Psychology University of AmsterdamNieuwe Achtergracht 129 B1018 WT AmsterdamNetherlands Department of Ecology and Evolutionary Biology 117 Eno HallPrinceton UniversityNJ 08544USA Institute of Ecology College of Urban and Environmental SciencesPeking UniversityBeijing 100871PR China Centre for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesXishuangbannaYunnan 666303PR China Te Kura Putaiao-School of Science Auckland University of TechnologyAucklandNew Zealand School of Psychological Sciences University of Western AustraliaPerth35 Stirling HwyCrawley WA 6009Australia nature Conservation and Landscape Ecology Biometry and Environmental System AnalysisUniversity of FreiburgGermany Cambridge Institute for medical Research University of CambridgeCambridge Biomedical CampusThe Keith Peters BuildingHills RoadCambridge CB20XYUK Future of Humanity Institute University of OxfordOxford OX11PTUK Faculty of Kinesiology Sportand RecreationUniversity of Alberta2-130V Van Vliet 21 Complex(University Hall)EdmontonAlberta T6G 2H9Canada Division of Population medicine School of MedicineCardiff UniversityCardiff CF144YSUK

Societal biosecurity–measures built into everyday society to minimize risks from pests and diseases–is an important aspect of managing epidemics and *** aimed to identify societal options for reducing the transmission and spread of respiratory *** used SARS-CoV-2(severe acute respiratory syndrome coronavirus 2)as a case study to meet the immediate need to manage the COVID-19 pandemic and eventually transition to more normal societal conditions,and to catalog options for managing similar pandemics in the *** used a‘solution scanning’*** read the literature;consulted psychology,public health,medical,and solution scanning experts;crowd-sourced options using social media;and collated comments on a ***,we present a list of 519 possible measures to reduce SARS-CoV-2 transmission and *** provide a long list of options for policymakers and businesses to consider when designing biosecurity plans to combat SARS-CoV-2 and similar pathogens in the *** also developed an online application to help with this *** encourage testing of actions,documentation of outcomes,revisions to the current list,and the addition of further options.

关键词： Biosecurity Coronavirus COVID-19 Disease control new normal Public health

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sex chromosomes behavior and G-banding treatment of male meiosis in nuptial gift-giving spiders of the family Trechaleidae

引用

Integrative Zoology 2011年第1期6卷 56-62页

作者： maria j.ALBO Alicia POSTIGLIOnI Laboratory of Ethology Ecology and EvolutionClemente Estable Biological Research InstituteMontevideoUruguay Department of Genetics and Animal Breeding Faculty of VeterinaryUniversity of the RepublicMontevideoUruguay

Preliminary genetic studies in Trechaleidae spider family show high variation in sex chromosomes and high heterocigocity,suggesting high chromatin *** trechaleids Paratrechalea ornata,Trechalea bucculenta and Trechaleoides biocellata are present in *** offering nuptial gifts during courtship have been reported in *** and *** but not in *** gifts are an inherited trait probably highly affected by environmental factors,which play an important role in gene *** hypothesize that this trait could be associated with tissue-specific genes existing in *** investigate the male meiosis in these 3 species,their sex chromosome system and the effects of G-banding on their chromosomes,and elucidate genetic differences among *** stages of the 3 species were submitted to Giemsa-staining and G-banding *** observed a haploid number of n=11 in *** and n=13 in both *** and *** from the 3 species presented an X_(1)X_(2)0 sex chromosome system,which is suggested as ancestral in *** *** and ***,both sex chromosomes were together and aligned in parallel until the segregation during anaphase *** contrast to these species,sex chromosomes of *** usually remained distant from each other until diakinesis when they were observed associated in parallel *** G-bands were similar in *** and ***,and they both differed from those in *** special behavior of sex chromosomes in *** as well as the different G-banding pattern of this species suggests the existence of novel modifications in this species.

关键词： G-banding male meiosis neotropical spiders sex chromosomes Trechaleidae

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：