Commitment of human mesenchymal stromal cells to skeletal lineages is independent of their morphogenetic capacity

作     者：Jessica Cristina Marín-Llera Damián García-García Estefania Garay-Pacheco Victor Adrian Cortes-Morales Juan Jose Montesinos-Montesinos Jesus Chimal-Monroy 

作者机构：Medicina Genómica y Toxicología AmbientalInstituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico Laboratorio de Células Troncales MesenquimalesUnidad de Investigación Médica en Enfermedades OncológicasHospital de Oncología Centro Medico Nacional Siglo XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Departamento de Medicina Genómica y Toxicología AmbientalInstituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2023年第15卷第7期

页      面：701-712页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：Supported by the Dirección General de Asuntos del Personal Académico(DGAPA)-Universidad Nacional Autónoma de México,No.IN211117 Consejo Nacional de Ciencia y Tecnología(CONACyT),No.1887 CONACyT-Fronteras de la Ciencia awarded to Chimal-Monroy J García-García RD and Garay-Pacheco E received an undergraduate scholarship Marin-Llera JC a postdoctoral fellowship from the Consejo Nacional de Ciencia y Tecnología,No.CONACyT-Fronteras de la Ciencia-1887 

主　　题：Human mesenchymal stromal cells Recombinant limbs Mesenchymal stromal cell morphogene-sis Mesenchymal stromal cell in vivo differentiation Skeletal tissues 

摘      要：BACKGROUND Mesenchymal stromal cells(MSCs)are multipotent cell populations obtained from fetal and adult *** share some characteristics with limb bud mesodermal cells such as differentiation potential into osteogenic,chondrogenic,and tenogenic lineages and an embryonic mesodermal *** MSCs differentiate into skeletal-related lineages in vitro,they have not been shown to selforganize into complex skeletal structures or connective tissues,as in the *** this work,we demonstrate that the expression of molecular markers to commit MSCs to skeletal lineages is not sufficient to generate skeletal elements in *** To evaluate the potential of MSCs to differentiate into skeletal lineages and generate complex skeletal structures using the recombinant limb(RL)*** We used the experimental system of RLs from dissociated-reaggregated human placenta(PL)and umbilical cord blood(UCB)*** being harvested and reaggregated in a pellet,cultured cells were introduced into an ectodermal cover obtained from an early chicken limb ***,this filled ectoderm was grafted into the back of a donor chick *** these conditions,the cells received and responded to the ectoderm’s embryonic signals in a spatiotemporal manner to differentiate and pattern into skeletal *** response to differentiation and morphogenetic signals was evaluated by quantitative poly-merase chain reaction,histology,immunofluorescence,scanning electron microscopy,and in situ *** We found that human PL-MSCs and UCB-MSCs constituting the RLs expressed chondrogenic,osteogenic,and tenogenic molecular markers while differentially committing into limb lineages but could not generate complex structures in ***-RL from PL or UCB were committed early to chondrogenic ***,the UCB-RL osteogenic commitment was favored,although preferentially to a tenogenic cell *** findings suggest that the commitment of MSCs to differentiate int