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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng ei ei Khine Khin Mar Thi ei ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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improved YOLOv8s-Based Night Vehicle Detection

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印刷与数字媒体技术研究 2024年第4期 76-85页

作者： WAN Xin-ei si Zhan-jun College of Artificial intelligence Tianjin University of Science and TechnologyTianjin 300457China

With the gradual development of automatic driving technology,people’s attention is no longer limited to daily automatic driving target *** response to the problem that it is difficult to achieve fast and accurate detection of visual targets in complex scenes of automatic driving at night,a detection algorithm based on improved YOLOv8s was ***,By adding Triplet Attention module into the lower sampling layer of the original model,the model can effectively retain and enhance feature information related to target detection on the lower-resolution feature *** enhancement improved the robustness of the target detection network and reduced instances of missed ***,the Soft-NMS algorithm was introduced to address the challenges of dealing with dense targets,overlapping objects,and complex *** algorithm effectively reduced false and missed positives,thereby improved overall detection performance when faced with highly overlapping detection ***,the experimental results on the MpDioU loss function dataset showed that compared with the original model,the improved method,in which mAp and accuracy are increased by 2.9%and 2.8%respectively,can achieve better detection accuracy and speed in night vehicle *** can effectively improve the problem of target detection in night scenes.

关键词： Vehicle detection Yolov8 Attention mechanism

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How Can practitioners Support Citizen Volunteers in Disaster Risk Reduction? insight from “Good and Ready” in Aotearoa New Zealand

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international Journal of Disaster Risk Science 2024年第03期15卷 374-387页

作者： Lo?c Le Dé Steve Ronoh ei Mon Thinn Kyu Brigitte Rive School of public Health and interdisciplinary Studies Auckland University of Technology pacific Health Research Centre Auckland University of Technology New Zealand Red Cross

Global and national policy frameworks emphasize the importance of people's participation and volunteers' role in disaster risk reduction. While research has extensively focused on volunteers in disaster response and recovery, less attention has been paid on how organizations involved in disaster risk management can support volunteers in leading and coordinating communitybased disaster risk reduction. in 2019, the New Zealand Red Cross piloted the Good and Ready initiative in Auckland,Aotearoa New Zealand, with the objective to empower local people in resilience building with a focus on volunteers and community participation. This research examined the positive and negative outcomes of Good and Ready and investigated volunteers' experiences in the disaster resilience initiative. it involved the codesign of a questionnaire-based survey using participatory methods with Good and Ready volunteers, the dissemination of the survey to gather volunteers' viewpoints, and a focus group discussion with participatory activities with Red Cross volunteers. The findings highlight that a key challenge lies in finding a balance between a program that provides flexibility to address contextual issues and fosters communities' ownership, versus a prescriptive and standardized approach that leaves little room for creativity and self-initiative. it pinpoints that supporting volunteers with technical training is critical but that soft skills training such as coordinating, communicating,or facilitating activities at the local level are needed. it concludes that the sustainability of Good and Ready requires understanding and meeting volunteers' motivations and expectations and that enhancing partnerships with local emergency management agencies would strengthen the program.

关键词： Community-based approach Disaster risk reduction New Zealand participation Volunteers

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Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

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BiOCeLL 2024年第4期48卷 613-621页

作者： YAO FANG Fei XiA FeiFei TiAN L ei QU FANG YANG JUAN FANG ZHeNHONG HU HAiCHAO LiU Department of Respiratory and Critical Care Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and engineering Southwest Jiaotong UniversityChengdu611756China

Objective:in the realm of Class i pathogens,Burkholderia pseudomallei(Bp)stands out for its propensity to induce severe *** the intricate interactions between Bp and host cells is imperative for comprehending the dynamics of Bp infection and discerning biomarkers indicative of the host cell response ***:mRNA extraction from Bp-infected mouse macrophages constituted the initial step of our *** gene expression arrays,the extracted RNA underwent conversion into digital *** percentile shift method facilitated data processing,with the identiﬁcation of genes manifesting signiﬁcant differences accomplished through the application of the ***,a comprehensive analysis involving Gene Ontology enrichment and Kyoto encyclopedia of Genes and Genomes pathway was conducted on the differentially expressed genes(DeGs).Leveraging the eSTiMATe algorithm,gene signatures were utilized to compute risk scores for gene expression *** vector machine analysis and gene enrichment scores were instrumental in establishing correlations between biomarkers and macrophages,followed by an evaluation of the predictive power of the identiﬁed ***:The functional and pathway associations of the DeGs predominantly centered around G protein-coupled receptors.A noteworthy positive correlation emerged between the blue module,consisting of 416 genes,and the ***4,identiﬁed through support vector machine analysis among intersecting genes,indicated a robust interaction with macrophages,suggesting its potential as a robust ***4 exhibited commendable predictive efﬁcacy,with Bp infection inducing its expression in both macrophages and mouse lung *** blotting in macrophages conﬁrmed a signiﬁcant upregulation of FZD4 expression from 0.5 to 24 h *** mouse lung tissue,FZD4 manifested higher expression in the cytoplasm of pulmonary epithelial cells in Bp-infected lungs than in the

关键词： Burkholderia pseudomallei Microarray assay Machine learning Bioinformatics FZD4

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Safety and efficacy of tramadol hydrochloride on treatment of premature ejaculation

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Asian Journal of Andrology 2013年第1期15卷 138-142页

作者： Bayoumy i eassa Mohamed A ei-Shazly Department of DermatoLogy Venereology and Andrology AI-Azhar University Cairo 11884 Egypt Department of Urology Menoufiya University Cairo 32511 Egypt

premature ejaculation （pe） is the most common sexual disorder. it affects 20%-30% of adult men; the aetiology of this condition has not yet been elucidated. The aim of this study is to evaluate the efficacy, safety, tolerability, undesirable effects and improved satisfaction with sexual intercourse with tramadol hydrochloride at different dosages for the treatment of pe. A total of 300 patients who presented with lifelong （primary） pe were included in this study. The study was performed for 28 weeks, in which placebo （starch tablet） was given for 4 weeks, and active ingredient （tramadol hydrochloride） was administered at different therapeutic dosages for 24 weeks. patients were divided into three equal groups, each consisting of 100 patients. The first group （A） was given tramadol hydrochloride capsule 25 mg. The second group （B） was given tramadol hydrochloride capsule 50 mg. The third group （C） was given tramadol hydrochloride capsule 100 mg. All of the 300 participants included completed the study voluntarily. The age of the patients varied from 25 to 50 years. After the treatment period, the recorded data were collected for each group and analysed. The results showed a highly significant increase in the mean intravaginal ejaculatory latency time （ieLT） in all groups compared to baseline data （p〈0.0001）. We concluded that using tramadol hydrochloride at different doses on demand for the treatment of pe is effective, safe and tolerable, with minimal undesirable effects, and approval for this indication should be souRht.

关键词： early ejaculation erectile dysfunction intravaginal ejaculation latencytime premature climax premature ejaculation pe rapid ejaculation tramadol

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Understanding of the Relationship between the properties of Cu(in,Ga)Se2 Solar Cells and the Structure of Ag Network electrodes

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eNeRGY & eNViRONMeNTAL MATeRiALS 2024年第6期7卷 246-253页

作者： Yoo, Hyesun Quy, Hoang Van Lee, inpyo Jo, Seung Taek Hong, Tae ei Kim, Junho Yoo, Dae-Hwang Shin, Jinwook Commerell, Walter Kim, Dae-Hwan Roh, Jong Wook Kyungpook Natl Univ Reg Leading Res Ctr RLRC Smart Energy Syst Daegu 37224 Gyeongsangbug D South Korea Kyungpook Natl Univ Dept Hydrogen & Renewable Energy Daegu 47922 South Korea Kyungpook Natl Univ Dept Nano & Adv Mat Sci & Engn Daegu 37224 Gyeongsangbug D South Korea Daegu Gyeongbuk inst Sci & Technol DGiST Div Energy Technol Daegu 42988 South Korea incheon Natl Univ Dept Phys Nano Photoelect Device Lab Incheon 22012 South Korea TH Ulm THU Prittwitzstr 10 D-89081 Ulm Germany

The relation between the structure of the silver network electrodes and the properties of Cu(in,Ga)Se2 (CiGS) solar cells is systemically investigated. The Ag network electrode is deposited onto an Al:ZnO (AZO) thin film, employing a self-forming cracked template. precise control over the cracked template's structure is achieved through careful adjustment of temperature and humidity. The Ag network electrodes with different coverage areas and network densities are systemically applied to the CiGS solar cells. it is revealed that predominant fill factor (FF) is influenced by the figure of merit of transparent conducting electrodes, rather than sheet resistance, particularly when the coverage area falls within the range of 1.3-5%. Furthermore, a higher network density corresponds to an enhanced FF when the coverage areas of the Ag networks are similar. When utilizing a thinner AZO film, CiGS solar cells with a surface area of 1.0609 cm2 exhibit a notable performance improvement, with efficiency increasing from 10.48% to 11.63%. This enhancement is primarily attributed to the increase in FF from 45% to 65%. These findings underscore the considerable potential for reducing the thickness of the transparent conductive oxide (TCO) in CiGS modules with implications for practical applications in photovoltaic technology.

关键词： CiGS large-area solar cell metal mesh metallic network transparent electrode

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry traces the geographical source of Biomphalaria pfeifferi and Bulinus forskali,involved in schistosomiasis transmission

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infectious Diseases of poverty 2024年第1期13卷 98-99页

作者： papa Mouhamadou Gaye ei Hadj ibrahima Ndiaye Souleymane Doucoure Doudou Sow Mapenda Gaye Ndiaw Goumballa Carole Cassagne Coralie L'Ollivier Oleg Medianikov Cheikh Sokhna Stephane Ranque Aix-Marseille University IRD AP-HM SSA VITROME Marseille France ViTROMe International IRD-UCAD Campus Dakar Senegal Hospital-University institut (iHU) Mediterranée infection Marseille France Departement of Animal Biology Faculty of Sciences and Techniques UCAD Dakar Senegal Department of parasitology-Mycology UFR Sciences de la Santé Université Gaston Berger Saint-Louis Senegal

Background Freshwater snails of the genera Bulinus spp.,Biomphalaria spp.,and Oncomelania *** the main intermediate hosts of human and animal *** of these snails has long been based on mor-phological and/or genomic criteria,which have their *** limitations include a lack of precision for the morphological tool and cost and time for the DNA-based ***,Matrix-Assisted Laser Desorp-tion/lonization Time-Of-Flight(MALDi-TOF)mass spectrometry,a new tool used which is routinely in clinical microbi-ology,has emerged in the field of malacology for the identification of freshwater *** study aimed to evaluate the ability of MALDi-TOF MS to identify Biomphalaria pfeifferi and Bulinus forskali snail populations according to their *** This study was conducted on 101 *** and 81 *** snails collected in three distinct geo-graphical areas of Senegal(the North-east,South-east and central part of the country),and supplemented with wild and laboratory *** which had previously been morphologically described were identified by MALDl-TOF MS[identification log score values(LSV)≥1.7],after an initial blind test using the pre-existing *** DNA-based identification,new reference spectra of ***(n=10)and ***(n=5)from the geographical areas were added to the MALDi-TOF spectral *** final blind test against this updated database was per-formed to assess identification at the geographic source *** MALDi-TOF MS correctly identified 92.1%of 101 *** snails and 98.8%of 81 *** *** the final blind test,88%of 166 specimens were correctly identified according to both their species and sampling site,with LSVs ranging from 1.74 to *** geographical source was adequately identified in 90.1%of 91 *** and 85.3%of 75 *** *** Our findings demonstrate that MALDi-TOF MS can identify and differe

关键词： Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Snail intermediate host Biomphalaria pfeifferi Bulinus forskali Schistosomiasis Senegal

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Therapeutic effect of Dimethyl Dimethoxy Biphenyl Dicarboxylate on Collagen-induced Arthritis in Rats

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Chinese Journal of integrative Medicine 2015年第11期21卷 846-854页

作者： Roba M Talaat Amira S Abo-ei-Atta Sabah M Farou Karima i ei-Dosoky Molecular Biology Department Genetic Engineering and Biotechnology Research InstituteMenofia UniversitySadat Branch pathology Department Faculty of MedicineTanta University

Objective： To study the effect of oral administration of dimethyl dimethoxy biphenyl dicarboxylate （DDB） on adjusting angiogeneic/inflammatory mediators and ameliorating the pathology of bones in rats with collagen-induced arthritis （CiA）. Methods： Wistar rat model of CiA was set up using bovine collagen type H. fifty rats were divided into five groups randomly： normal, CiA model, DDB treatment, methotrexate （MTX） treatment, and combined DDB＋MTX treatment. Ankle joints of rats were imaged with digital X-ray machine to show the destruction of joints. Fore and hind paw and knee joints were removed above the ankle joint then processed for haematoxylin and eosin staining. plasma levels of vascular endothelial growth factor （VeGF）, platelet derived growth factor, interleukin-8 （iL-8）, iL-4, tumor necrosis factor α （TNF-α）, and cyclooxygenase-2 （COX-2） were quantified by enzyme-linked immunosorbent assay. Nitric oxide levels were detected by Griess reagent. Results： Compared with the CiA model group, a remarkable reduction in various angiogenic （VeGF and iL-8） and inflammatory mediators （TNF-α, iL-4 and COX-2） after treatment with DDB either alone or combined with MTX （p〈0.05 or p〈0.01）. Histopathological and X-ray findings were confirmatory to the observed DDB anti-arthritic effect. The DDB-treated group showed amelioration in signs of arthritis which appeared essentially similar to normal. Conclusion： Our data shed light on the therapeutic efficacy of DDB in experimental rheumatoid arthritis （RA） compared with a choice drug （MTX） and it may be offered as a second-line drug in the treatment of RA.

关键词： rheumatoid arthritis collagen-induced arthritis dimethyl dimethoxy biphenyl dicarboxylate angiogenesis inflammation

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对比与融合巴塞罗那***光学精品店

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室内设计与装修 2024年第5期 82-85页

作者： ei Departamento ei Departamento 西班牙

el Departamento设计工作室以其独到的创意,为全渠道眼镜零售品牌***在巴塞罗那Born区的门店注入了新的生机。该空间以精致的单色内饰引人注目,通过巧妙的布局和色彩运用,营造出动态且富有吸引力的实体零售环境。这不仅仅是一个购物空间... 详细信息

el Departamento设计工作室以其独到的创意,为全渠道眼镜零售品牌***在巴塞罗那Born区的门店注入了新的生机。该空间以精致的单色内饰引人注目,通过巧妙的布局和色彩运用,营造出动态且富有吸引力的实体零售环境。这不仅仅是一个购物空间,更是一个让顾客能够亲身体验品牌精神和设计理念的场所。同时,该店铺以其独特的设计风格,成功地从众多参赛作品中脱颖而出。

关键词：设计工作室巴塞罗那购物空间品牌精神亲身体验全渠道色彩运用设计理念

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peak-to-average power ratio reduction using selective mapping with unequal power distribution

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Journal of Central South University 2012年第7期19卷 1902-1908页

作者： e.S.Hassan XU Zhu S.e.e1-Khamy M.i.Dessouky S.A.ei-Dolill F.e.Abd ei-Samie Department of electronics and electrical Communications Faculty of Electronic EngineeringMenoufia UniversityMenouf 32952Egypt Department of electrical engineering and electronics The University of LiverpoolLiverpool L693G JUK Department of electrical engineering Faculty of EngineeringAlexandria UniversityAlexandria 21544Egypt

A new approach for peak-to-average power ratio (pApR) reduction in orthogonal frequency division multiplexing (OFDM) systems was *** approach is based on assigning powers to the different subcarriers of OFDM using an unequal power distribution *** addition,a reduced complexity selective mapping (RC-SLM) scheme was *** proposed scheme is based on partitioning the frequency domain symbol sequence into several sub-blocks,and then each sub-block is multiplied by different phase sequences whose length is shorter than that used in the conventional SLM ***,a kind of low complexity conversions is used to replace the iFFT *** performance of the proposed RC-SLM scheme along with the new approach was studied with computer *** obtained results show that the proposed RC-SLM scheme is able to achieve the lowest computational complexity when compared with other low complexity schemes proposed in the literature while at the same time improves the pApR reduction performance by about 0.3 dB.

关键词： orthogonal frequency division multiplexing (OFDM) peak-to-average power ratio (pApR) computational complexity selective mapping scheme

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