Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry traces the geographical source of Biomphalaria pfeifferi and Bulinus forskali,involved in schistosomiasis transmission

作     者：Papa Mouhamadou Gaye EI Hadj Ibrahima Ndiaye Souleymane Doucoure Doudou Sow Mapenda Gaye Ndiaw Goumballa Carole Cassagne Coralie L'Ollivier Oleg Medianikov Cheikh Sokhna Stephane Ranque 

作者机构：Aix-Marseille University IRD AP-HM SSA VITROME Marseille France VITROME International IRD-UCAD Campus Dakar Senegal Hospital-University Institut (IHU) Mediterranée Infection Marseille France Departement of Animal Biology Faculty of Sciences and Techniques UCAD Dakar Senegal Department of Parasitology-Mycology UFR Sciences de la Santé Université Gaston Berger Saint-Louis Senegal 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2024年第13卷第1期

页      面：98-99页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：Département de Soutien et Formation Fondation Méditerranée Infection Institut de Recherche pour le Développement, IRD, (DA08022018) Institut de Recherche pour le Développement, IRD 

主　　题：Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Snail Intermediate host Biomphalaria pfeifferi Bulinus forskali Schistosomiasis Senegal 

摘      要：Background Freshwater snails of the genera Bulinus spp.,Biomphalaria spp.,and Oncomelania *** the main intermediate hosts of human and animal *** of these snails has long been based on mor-phological and/or genomic criteria,which have their *** limitations include a lack of precision for the morphological tool and cost and time for the DNA-based ***,Matrix-Assisted Laser Desorp-tion/lonization Time-Of-Flight(MALDI-TOF)mass spectrometry,a new tool used which is routinely in clinical microbi-ology,has emerged in the field of malacology for the identification of freshwater *** study aimed to evaluate the ability of MALDI-TOF MS to identify Biomphalaria pfeifferi and Bulinus forskali snail populations according to their *** This study was conducted on 101 *** and 81 *** snails collected in three distinct geo-graphical areas of Senegal(the North-East,South-East and central part of the country),and supplemented with wild and laboratory *** which had previously been morphologically described were identified by MALDl-TOF MS[identification log score values(LSV)≥1.7],after an initial blind test using the pre-existing *** DNA-based identification,new reference spectra of ***(n=10)and ***(n=5)from the geographical areas were added to the MALDI-TOF spectral *** final blind test against this updated database was per-formed to assess identification at the geographic source *** MALDI-TOF MS correctly identified 92.1%of 101 *** snails and 98.8%of 81 *** *** the final blind test,88%of 166 specimens were correctly identified according to both their species and sampling site,with LSVs ranging from 1.74 to *** geographical source was adequately identified in 90.1%of 91 *** and 85.3%of 75 *** *** Our findings demonstrate that MALDI-TOF MS can identify and differe