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Pinellia Total Alkaloids Modulate the GABAergic System in Hippocampal Formation on Pilocarpine-Induced Epileptic Rats

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Chinese Journal of Integrative Medicine 2020年第2期26卷 138-145页

作者： deng Chu-xin WU Zhi-bing CHEN Yi YU Zheng-miao The First Clinical Medical School Guangzhou University of Chinese MedicineGuangzhou(510406)China Cerebropathy Center The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou(510406)China

Objective:To investigate the neuromodulatory effect of pinellia total alkaloids(PTA)on the gamma-aminobutyric acidergic(GABAergic)system in epileptic rats,and preliminarily evaluate the anti-epileptic effect of ***:Ninety-one male Sprague-Dawley rats were randomized to a control group(n=17)or an epileptic group(n=74)using computer-generated random *** epilepticus(SE)was induced with pilocarpine in the epileptic *** rats that survived SE were randomly divided into 4 groups,namely an epilepsy group(n=13),a topiramate(TPM,60 mg/kg)group(n=12),a high-dose PTA(800 mg/kg)group(n=12),and a low-dose PTA(400 mg/kg)group(n=10).Treatments were given intragastrically once daily for 14 *** control group and epilepsy group received normal *** recurrent seizures(SRSs)were monitored 8-h daily for 7 days after ***,the hippocampal formation tissues were *** level was measured using enzyme-linked immunosorbent *** and mRNA expression levels of glutamate decarboxylase 65(GAD65),GABA transporter-1(GAT-1),GABA transaminase(GABA-T),and GABAA receptor(GABAAR)α4,α5,γ2 andδsubunits were measured using Western-blotting analysis and quantitative polymerase chain ***:PTA lowered the incidence and frequency of SRS(both doses *** TPM group,P>0.05).Compared with the epilepsy group,PTA increased the levels of GABA(both doses P<0.01)and GAD65(mRNA,800 mg/kg,P<0.01),and suppressed the levels of GAT-1(mRNA,800 mg/kg,P<0.01;400 mg/kg,P<0.05),GABA-T(mRNA,both doses P<0.01),and GABAARδsubunit(protein,800 mg/kg,P<0.05)andγ2 subunit(protein,both doses P<0.01).PTA upregulated the low-expressed mRNA levels of GABAARα5 subunit(400 mg/kg,P<0.01),δsubunit(800 mg/kg,P<0.05),andγ2 subunit(400 mg/kg,P<0.05).Conclusions:PTA regulated the GABAergic system through modulating GABA levels and the expression levels of GAD65,GAT-1,GABA-T,and GABAARα4,α5,γ2 andδ*** may exert antiepileptic effects on the pilocarpine-

关键词： Pineilia total alkaloids Pineilia ternata gamma-aminobutyric acidergic agents seizure epilepsy

Rock physical characteristics of deep dolomite under complex geological conditions:A case study of 4th Member of Sinian dengying Formation in the Sichuan Basin,China

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Petroleum Science 2024年第4期21卷 2370-2382页

作者： Chuang Li Shu-xin Pan Hong-Bin Wang Ji-xin deng Jian-Guo Zhao Zhi Li Yu Zhang Northwest Branch of Research Institute of Petroleum Exploration&Development Lanzhou730020GansuChina State Key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing102249China Department of Geophysics and Exploration College of GeophysicsChengdu University of TechnologyChengdu610059SichuanChina

The deep-ultra deep carbonate reservoir in China,commonly subjected to modification of multi-stage diagenesis,has extremely high *** rock physics analysis cannot accurately identify the elastic responses of ***,the rock physics properties of the dolomite from the 4th Member of the Sinian dengying Formation are experimentally measured,and the change law of rock physics characteristics is investigated within the framework of the diagenetic processes by the analysis of the elastic and petrologic characteristics,pore structure,and sedimentary *** results show that the differentiated diagenesis results in different pore structure characteristics and microtexture characteristics of the *** microbial dolomite of the algal mound-grain beach facies is subjected to the contemporaneous microbial dolomitization and seepage-reflux dolomitization,penecontemporaneous selective dissolution,burial dolomitization,and hydrothermal *** resultant crystalline dolomite is found with one main type of the dolomite crystal contact boundaries,and the dissolution pore is extensive *** siliceous,muddy,and limy dolomite of the interbeach sea environment mainly experiences the weak capillary concentration dolomitization,intensive mechanical compaction-induced densification,and burial *** crystalline dolomite is observed with four types of contact boundaries,namely the dolomite contact,clay contact,quartz contact,and calcite contact boundaries,and porosity mostly attributed to residual primary inter-granular or crystalline *** samples with the same crystal boundary condition have consistent correlations between the compressional-and shear-wave velocities,and between the compressional-wave velocity and the velocity ***,the variation of the acoustic velocity with effective pressure and the intensity of pore-scale fluid-related dispersion are controlled by the differentiation of pore st

关键词： Deep-ultra deep carbonate reservoirs Rock physics properties Dolomite Seismic elastic properties Micro-crack Pore structure types

Gold nanoflowers for 3D volumetric molecular imaging of tumors by photoacoustic tomography

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Nano Research 2015年第7期8卷 2152-2161页

作者： Yuanyuan Jiang Zijian deng Dan Yang xin deng Qi Li Yinlin Sha Changhui Li Dongsheng Xu Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable Species College of Chemistry and Molecular Engineering Peking University Beijing 100871 China Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China Single-molecule and Nanobiology Laboratory Department of Biophysics School of Basic Medical Sciences and Biomed-X Center Peking University Beijing 100191 China These authors contributed equally to this work.

By binding molecular probes that target tumor cells, gold nanoparticles （AuNPs） with superior characteristics have shown great potential in tumor molecular imaging studies. The non-invasive, high-resolution, and three-dimensional imaging of the targeted AuNPs within the tumor is desirable for both diagnosis and therapy. In this study, gold nanoflowers （AuNFs） are presented as a novel contrast agent for photoacoustic tomography （PAT）. By binding to folic acid, the molecular probe, the tail-vein injected AuNFs concentrated within the tumor site in mice; this was clearly visualized by three-dimensional （3D） PAT imaging. In addition, toxicity assay proved that AuNFs were harmless to living cells and animals. Our results demonstrate that AuNFs have great potential in tumor molecular imaging.

关键词： nanoparticles,gold nanoflowers,contrast agents,photoacoustic tomography tumor imaging,molecular imaging

Induction and Genetic Identification of Embryogenic Calli from Hybrids of Shatian Pummelo

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Agricultural Sciences in China 2006年第8期5卷 591-595页

作者： SONG Jian-kun deng Xiu-xin National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China

Shatian pummelo （Citrus grandis L. Osbeck cv. Shatian） is an elite variety in China, and the regeneration of the embryogenic callus is difficult. Diploid Shatian pummelo was used as the female and crossed with the allotetraploid somatic hybrid NS （Nova Tangelo ＋ Succari Sweet orange）, [ （ C reticulata Blanco x C. paradisi Macf.） cv. Nova ＋ C sinensis L. Osbeck cv. Succari]. About 90 days after pollination, the embryos obtained from crosses were cultured on the solid media of MT ＋ ME （malt extraction, 500 mg L^-1） and MT ＋ GA3 （1 mg L^-1）. The embryogenic callus was initiated from the embryoids and plantlets＇ hypocotyls and could be subcultured. Flow cytometry and SSR analysis verified that the callus was from the triploid hybrids. The callus had embryogenesis capacity and produced a large number of embryoids on MT ＋Lactose （50 g L^-1） medium after being subcultured for two years. It is comparatively easier to obtain the callus from the hybrid embryo than from Shatian pummelo itself. The callus is valuable for the conservation and utilization of Shatian pummelo.

关键词： Shatian pummelo embryogenic callus flow cytometry SSR

Integrated network analysis of transcriptomic and protein-protein interaction data in taurine-treated hepatic stellate cells

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World Journal of Gastroenterology 2019年第9期25卷 1067-1079页

作者： xing-Qiu Liang Jian Liang Xiao-Fang Zhao xin-Yuan Wang xin deng Department of Science and Technology Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine College of Medical Guangxi University School of Basic Sciences Guangxi University of Chinese Medicine

BACKGROUND Studies show that the antifibrotic mechanism of taurine may involve its inhibition of the activation and proliferation of hepatic stellate cells(HSCs). Since the molecular mechanism of taurine-mediated antifibrotic activity has not been fully unveiled and is little studied, it is imperative to use "omics" methods to systematically investigate the molecular mechanism by which taurine inhibits liver *** To establish a network including transcriptomic and protein-protein interaction data to elucidate the molecular mechanism of taurine-induced HSC *** We used microarrays, bioinformatics, protein-protein interaction(PPI) network,and sub-modules to investigate taurine-induced changes in gene expression in human HSCs(LX-2). Subsequently, all of the differentially expressed genes(DEGs) were subjected to gene ontology function and Kyoto encyclopedia of genes and genomes pathway enrichment analysis. Furthermore, the interactions of DEGs were explored in a human PPI network, and sub-modules of the DEGs interaction network were analyzed using Cytoscape *** A total of 635 DEGs were identified in taurine-treated HSCs when compared with the controls. Of these, 304 genes were statistically significantly up-regulated, and 331 down-regulated. Most of these DEGs were mainly located on the membrane and extracellular region, and are involved in the biological processes of signal transduction, cell proliferation, positive regulation of extracellular regulated protein kinases 1(ERK1) and ERK2 cascade, extrinsic apoptotic signaling pathway and so on. Fifteen significantly enriched pathways with DEGs were identified, including mitogen-activated protein kinase(MAPK) signaling pathway, peroxisome proliferators-activated receptor signaling pathway,estrogen signaling pathway, Th1 and Th2 cell differentiation, cyclic adenosine monophosphate signaling pathway and so on. By integrating the transcriptomics and human PPI data, nine critical genes, inclu

关键词： Taurine Hepatic stellate cells Differentially expressed genes Liver fibrogenesis Transcriptomic Protein-protein interaction network

Depressive symptoms and their associated factors in heart failure patients

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Frontiers of Nursing 2021年第4期8卷 389-397页

作者： deng-xin He Ming-Hao Pan School of Nursing Hubei University of Chinese MedicineWuhanHubei 430065China School of Medicine Xinyang Normal UniversityXinyangHenan 464000China

Objectives:Depressive symptoms are common in heart failure(HF)patients and they may exacerbate the progression of ***,identifying associations with depressive symptoms is essential to develop effective interventions to alleviate patients’depressive ***,this study aimed to explore the factors related to HF patients’depressive ***:Potential hospitalized patients were recruited from a university-affiliated hospital by convenience *** study included 321 HF patients who had completed information about depressive symptoms,functional capacity,social support,dispositional optimism,self-care confidence,and health *** statistics were used to characterize the *** group t-tests and one-way analysis of variance were used to assess the difference in depressive symptoms score in demographic and clinical characteristics,while Pearson’s correlation was used to assess the associations among continuous ***:The scores for functional capacity,social support,dispositional optimism,self-care confidence,and health literacy were negatively associated with the score for depressive *** interaction effect between the functional capacity and the dispositional optimism on depressive symptoms was ***:Interventions targeted improving the above-mentioned factors may be beneficial to reduce depressive symptoms in HF patients.

关键词： depressive symptoms dispositional optimism functional capacity heart failure social support

On the strong convergence properties for weighted sums of negatively orthant dependent random variables

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Applied Mathematics(A Journal of Chinese Universities) 2018年第1期33卷 35-47页

作者： deng xin TANG Xu-fei WANG Shi-jie WANG Xue-jun School of Mathematical Sciences Anhui University

In the paper, the strong convergence properties for two different weighted sums of negatively orthant dependent(NOD) random variables are investigated. Let {X, n ≥ 1}be a sequence of NOD random variables. The results obtained in the paper generalize the corresponding ones for i.i.d. random variables and identically distributed NA random variables to the case of NOD random variables, which are stochastically dominated by a random variable X. As a byproduct, the Marcinkiewicz-Zygmund type strong law of large numbers for NOD random variables is also obtained.

关键词： strong convergence negatively orthant dependent random variables stochastic domination

Positive Associations between Bilateral High-Frequency Hearing Loss and Hypertension Risk in Short-Term Occupational Noise Exposure

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Biomedical and Environmental Sciences 2021年第12期34卷 992-997页

作者： JIN Nan ZHONG Hui Yun YUAN Fang deng Hua xin Department of Occupational Health and Radiation Health Chongqing Center for Disease Control and PreventionChongqing 400042China Xiejiawan Street Community Health Service Center Chongqing 400042China

Noise is one of the most common occupational hazards worldwide[1], and millions of workers are exposed to harmful levels of noise in the workplace[2]. Noise-induced hearing loss(NIHL) is a leading cause of occupational and recreational injury and disease[3]. There is evidence that occupational noise exposure is also associated with other health effects, such as hypertension and cardiovascular diseases, digestive disorders, behavioral disorders.

关键词： occupational cardiovascular diseases

Synaptic aging disrupts synaptic morphology and function in cerebellar Purkinje cells

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Neural Regeneration Research 2018年第6期13卷 1019-1025页

作者： Wen-Juan Fan Ming-Chao Yan Lai Wang Yi-Zheng Sun Jin-Bo deng Jie-xin deng Institute of Neurobiology School of Life Science Henan University

Synapses are key structures in neural networks,and are involved in learning and memory in the central nervous *** synaptogenesis and synaptic aging is important in understanding neural development and neural degeneration in diseases such as Alzheimer disease and Parkinson’s *** previous study found that synaptogenesis and synaptic maturation were harmonized with brain development and ***,synaptic damage and loss in the aging cerebellum are not well *** study was designed to investigate the occurrence of synaptic aging in the cerebellum by observing the ultrastructural changes of dendritic spines and synapses in cerebellar Purkinje cells of aging ***,Di I diolistic assays,and transmission electron microscopy were used to visualize the morphological characteristics of synaptic buttons,dendritic spines and synapses of Purkinje cells in mice at various *** synaptic aging in the cerebellum,dendritic spines and synaptic buttons were lost,and the synaptic ultrastructure was altered,including a reduction in the number of synaptic vesicles and mitochondria in presynaptic termini and smaller thin specialized zones in pre-and post-synaptic *** findings confirm that synaptic morphology and function is disrupted in aging synapses,which may be an important pathological cause of neurodegenerative diseases.

关键词： nerve regeneration aging cerebellum degenerative disease dendritic spine nerve regeneration mice neurodegenerative diseases Purkinje cells synapse synaptogenesis synaptic ultrastructure neural regeneration