检索结果-南通市图书馆

Construction and function of recombinant AcMNPV with double copies of v-cath gene

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2002年第1期47卷 52-57页

作者： deli liu Huazhong Xiao Yipeng Qi Lunguang Yao Wuhan Univ Inst Virol Wuhan 430072 Peoples R China

Two recombinant baculoviruses, dciAcMNPV and dcdAcMNPV in which another copy of the v-cath gene controlled by ie1 promoter and polh promoter was inserted, were respectively constructed by the Bac-to-Bac system. The expression of the v-cath gene of the recombinant baculovi-ruses in Sf9 cells at different phases was investigated by SDS-PAGE and Western blot. The results showed that only re-combinant virus dciAcMNPV containing late gene v-cath driven by early gene promoter could express V-CATH pro-tein, cathepsin encoded by virus genome, 12 h post-infection and dcdAcMNPV containing late gene v-cath driven by late and very late gene promoters could express more V-CATH protein. Negative control ncAcMNPV, a mutant deleted v-cath gene, could not express V-CATH protein at all. The Spodopera exigua larvae were infected with viruses respec-tively and the results showed that the toxicity was as follows: dcdAcMNPV>dciAcMNPV>wtAcMNPV>ncAcMNPV. The toxicity of recombinant viruses and the characters of dead larvae

关键词： v-cath gene Western blot Bac-to-Bac system recombi-nant baculovirus.

Extraction of Surface Roughness from the Central δ-peak of Light Scattering Using a Multi-wavelength Ar~+ Laser

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Lasers 2000年第3期9卷 22-27页

作者： liu deli QI Dongping CHENG Chuanfu (Department of Physics, Shandong Normal University, Jinan 250014, China) 山东师范大学山东济南

In this paper, a new light scattering method for measuring t he roughness of random surfaces is proposed. The method is based on the relation between the specularly reflected intensity I s, i.e., the central δ-peak intensity and wave vector component k z. The linear fit of the logarithm graph of the normalized central δ-peak intensity I sr versus k-2 z, whose variation is induced by changing illuminating wavelength, gives the square of the surface roughness w. The roughnesses of 6 silicon backside samples were measured.

关键词： random surface roughness light scattering multi-wavelength

The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2005年第4期48卷 307-310页

作者： WANG Yan CONG Xiaoqian liu deli ZHANG Wenjie CUI Lei liu Wei CAO Yilin Department of Plastic and Reconstructive Surgery Shanghai 9th People's HospitalShanghai Second Medical UniversityShanghai Key Laboratory of Tissue EngineeringShanghai 200011China

We transiently transfected pTet-on and pTRE2hyg-luciferase into the mouse em-bryonic stem cells(ESCs)using lipofectamine,and analyzed its inductive effect by adding serial concentrations of doxycycline(DOX).The results showed that in the transfected group,the luciferase activity of the cells was gradually increased along with the increasing concentration of *** in the non-transfected group,the luciferase activity was not detectable even with DOX *** indicated that the ESCs transfected with Tet-on system could response to DOX very well,and the regulation of target gene expression is dose dependent.

关键词： gene expression embryonic stem cell.

Blue-White Colony Selection of Virus-Infected Isogenic Recipients Based on a Chrysovirus Isolated from Penicillium italicum

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第6期34卷 688-700页

作者： Tingfu Zhang Na Li Yongze Yuan Qianwen Cao Yanfen Chen Binglan Tan Guoqi Li deli liu Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079China Yunnan Higher Education Institutions College of Life Science and TechnologyHonghe UniversityMengzi 661199China

Mycoviruses have been found to infect more than 12 species of Penicillium, but have not been isolated from Penicillium italicum(P. italicum). In this study, we isolated and characterized a new double-stranded RNA(ds RNA) virus, designated Penicillium italicum chrysovirus 1(Pi CV1), from the citrus pathogen P. italicum HSPi-YN1. Viral genome sequencing and molecular characterization indicated that Pi CV1 was highly homologous to the previously described Penicillium chrysogenum virus. We further constructed the mutant HSPi-YN1 Dpks P defective in the polyketide synthase gene(pks P), which is involved in pigment biosynthesis, and these mutants formed albino(white) colonies. Then we applied hyphal anastomosis method to horizontally transmit Pi CV1 from the white virus-donors(i.e., HSPi-YN1 mutants) to wild-type recipients(i.e., *** strains HSPi-CQ54, HSPi-HB4, and HSPi-HN1), and the desirable Pi CV1-infected isogenic recipients, a certain part of blue wild-type strains, can be eventually selected and confirmed by viral genomic ds RNA profile analysis. This bluewhite colony screening would be an easier method to select virus-infected P. italicum recipients, according to distinguishable color phenotypes between blue virus-recipients and white virus-donors. In summary, the current work newly isolated and characterized Pi CV1, verified its horizontal transmission among dually cultured P. italicum isolates, and based on these, established an effective and simplified approach to screen Pi CV1-infected isogenic recipients.

关键词： Penicillium italicum chrysovirus 1(PiCV1) pksP knockout White-blue colony screening Isogenic recipients Horizontal transmission

Population diversity and phylogeny of halophiles in the Qinghai Lake and characterization and phylogenetic analysis of ectoine biosynthesis genes from Halomonas ***1

在线全文

学校读者我要写书评

暂无评论

Population diversity and phylogeny of halophiles in the Qing...

泡沫电热合金陶瓷涂层的制备及其高温氧化行为

生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： Derui Zhu Jian liu Rui Han deli liu College of Life Science Central China Normal University Medical College of Qinghai University Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life ScienceCentral China Normal University

Qinghai Lake is the largest inland saline lake in China,but the halophile population in wa-ter remains *** isolated 35 bacterial strains from different water samples in Qinghai Lake by using a RM *** majority of halophiles in this lake were the mod-erate halophiles accounting for about 62.85%,followed by the slight halophile(～22.85%),while the nonhalophile and halotolerant occupied 14.28%.We investigated and analyzed the population diversity and evolutionary relationship based on the 16S rDNA sequences.A total of 35 bacterial strains were sequenced,and homology analysis showed that the majority were affiliated

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

材料研究学报 2017年第8期31卷 603-611页

作者：胡晓娜段德莉刘俊李曙中国科学院金属研究所沈阳110016 上海空间推进研究所上海201112

利用真空浸渍和高温熔烧方法在泡沫Ni Cr20电热合金表面制备了陶瓷涂层,研究了其在1000℃的恒温氧化行为和热震行为,通过拉伸实验评价了涂层对氧向合金中扩散的阻挡效果。试验结果表明,在构成泡沫结构的金属棱内外表面得到均匀致密的陶... 详细信息

利用真空浸渍和高温熔烧方法在泡沫Ni Cr20电热合金表面制备了陶瓷涂层,研究了其在1000℃的恒温氧化行为和热震行为,通过拉伸实验评价了涂层对氧向合金中扩散的阻挡效果。试验结果表明,在构成泡沫结构的金属棱内外表面得到均匀致密的陶瓷涂层,涂层薄且与基体结合良好,能显著提高泡沫Ni Cr20电热合金在1000℃的恒温氧化性能和抗热震性能,表观拉伸强度保持在2 MPa。

关键词：金属材料泡沫电热合金陶瓷涂层真空浸渍熔烧恒温氧化性能热震性能拉伸性能

在线全文

学校读者我要写书评

暂无评论

动态白光数字散斑计量术

中国激光 2000年第7期27卷 649-654页

作者：亓东平程传福滕树云刘德丽山东师范大学物理系济南250014

提出了动态白光数字散斑计量术 ,并研制了相应的实验测量系统。在系统启动脉冲的触发下 ,动态白光数字散斑计量系统中物体动态加载、脉冲白光光源照明、数字散斑图像采集等过程依次完成。该系统可通过延时调节 ,对可重复动态过程的位移... 详细信息

提出了动态白光数字散斑计量术 ,并研制了相应的实验测量系统。在系统启动脉冲的触发下 ,动态白光数字散斑计量系统中物体动态加载、脉冲白光光源照明、数字散斑图像采集等过程依次完成。该系统可通过延时调节 ,对可重复动态过程的位移演化进行全过程测量。

关键词：白光数字散斑计量术激光

基于最小理论支撑体积的模型三维打印的打印方向分析

在线全文

学校读者我要写书评

暂无评论

中国机械工程 2019年第7期30卷 864-871页

作者：姜晓通郭保苏彭庆金刘德利常熟理工学院机械工程学院常熟215500 燕山大学机械工程学院秦皇岛066044 加拿大曼尼托巴大学机械工程学院加拿大曼尼托巴

针对三维打印过程中,打印后模型的支撑体积需减小的问题,研究了模型打印方向与模型支撑体积的对应关系,提出了一种计算最小理论支撑体积所对应的模型打印方向的方法。介绍了离散网格模型理论支撑体积的理论背景,给出了理论支撑体积的计... 详细信息

针对三维打印过程中,打印后模型的支撑体积需减小的问题,研究了模型打印方向与模型支撑体积的对应关系,提出了一种计算最小理论支撑体积所对应的模型打印方向的方法。介绍了离散网格模型理论支撑体积的理论背景,给出了理论支撑体积的计算方法;分析了理论支撑体积函数的可导性,针对该函数的不可导性,利用无导数优化算法计算了最小理论支撑体积所对应的打印方向。实验表明,所提出的方法能够准确地计算理论支撑体积所对应的打印方向。

关键词：三维打印打印方向理论支撑体积无导数优化算法

BFV-Blockchainvoting:支持BFV全同态加密的区块链电子投票系统

在线全文

学校读者我要写书评

暂无评论

通信学报 2022年第9期43卷 100-111页

作者：杨亚涛刘德莉刘培鹤曾萍肖嵩西安电子科技大学通信工程学院陕西西安710071 北京电子科技学院电子与通信工程系北京100070

当前的电子投票系统大多依赖于中心服务器和可信第三方,这种系统架构增加了投票的安全隐患,甚至使投票可能失败。为了解决这一问题,将区块链技术应用于电子投票系统,使区块链代替可信第三方,提出了一种支持BFV全同态加密的区块链电子投... 详细信息

当前的电子投票系统大多依赖于中心服务器和可信第三方,这种系统架构增加了投票的安全隐患,甚至使投票可能失败。为了解决这一问题,将区块链技术应用于电子投票系统,使区块链代替可信第三方,提出了一种支持BFV全同态加密的区块链电子投票系统BFV-Blockchainvoting。首先,用一个公开透明的公告板记录选票信息,同时设计了智能合约来实现验证、自计票功能;其次,为进一步提高投票过程的安全可靠性,使用SM2签名算法对投票者的注册信息进行签名处理,再选择能够互相监督的双方共同监管选票,并使用BFV同态加密算法来隐藏计票数据。经过测试与分析,所提系统单张选票的计票时间平均为1.69ms。所提方案可以为投票过程中的不可操纵性、匿名性、可验证性、不可重用性、不可胁迫性和抗量子攻击等安全属性提供保障,适用于多种投票场合,并且可以满足大型投票场景下的高效率需求。

关键词：电子投票区块链全同态加密 BFV同态加密智能合约

同方期刊数据库