The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline

作     者：WANG Yan CONG Xiaoqian LIU Deli ZHANG Wenjie CUI Lei LIU Wei CAO Yilin 

作者机构：Department of Plastic and Reconstructive SurgeryShanghai 9th People's HospitalShanghai Second Medical UniversityShanghai Key Laboratory of Tissue EngineeringShanghai 200011China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2005年第48卷第4期

页      面：307-310页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：This work was supported by the Major State Basic Research Development Program of China(Grant No.G1999054304) Shanghai Science and Technology Development Foundation(Grant No.03DJ14021) 

主　　题：gene expression embryonic stem cell. 

摘      要：We transiently transfected pTet-on and pTRE2hyg-luciferase into the mouse em-bryonic stem cells(ESCs)using lipofectamine,and analyzed its inductive effect by adding serial concentrations of doxycycline(DOX).The results showed that in the transfected group,the luciferase activity of the cells was gradually increased along with the increasing concentration of *** in the non-transfected group,the luciferase activity was not detectable even with DOX *** indicated that the ESCs transfected with Tet-on system could response to DOX very well,and the regulation of target gene expression is dose dependent.