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Sevoflurane effects on cyclic adenosine monophosphate response element binding protein,phosphorylated cyclic adenosine monophosphate response element binding protein,and Livin expression in the cortex and hippocampus of a vascular cognitive impairment rat

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Neural Regeneration Research 2009年第7期4卷 523-529页

作者： Bin Wu Ling dan Xianlin Zhu Department of Anesthesiology First Affiliated Hospital Chongqing Medical University Chongqing 400016 China Department of Anesthesiology Second Affiliated Hospital Chongqing Medical University Chongqing 400010 China

BACKGROUND： Neuronal necrosis and apoptosis play important roles in the pathophysiology of cerebral ischemia and resulting cognitive impairment. However, inhibition of neuronal necrosis and apoptosis has been shown to attenuate cognitive impairment following cerebral ischemia. OBJECTIVE： To investigate the effects of sevoflurane on cyclic adenosine monophosphate response element binding protein （CREB）, phosphorylated CREB （pCREB）, and Livin expression in the cortex and hippocampus of a rat model of vascular cognitive impairment.DESIGN, TIME AND SETTING： A randomized, controlled experiment was performed in the Chongqing Key Laboratory of Neurology between June 2007 and July 2008.MATERIALS： Sevoflurane was provided by Abbott Laboratory, UK; morris water maze was provided by Chinese Academy of Medical Sciences, China; goat anti-rat CREB, goat anti-rat pCREB and goat anti-rat Livin antibodies were provided by Biosource International, USA. METHODS： A total of 42 female, Wistar rats were randomly assigned to the following groups： sham operation, vascular cognitive impairment, and sevoflurane treatment. The vascular cognitive impairment rat model was established by permanent bilateral occlusion of both common carotid arteries, and 1.0 MAC sevoflurane was immediately administered by inhalation for 2 hours. MAIN OUTCOME MEASURES： CREB, pCREB, and Livin expression was measured in the cortex and hippocampus by Western blot and reverse transcription-polymerase chain reaction. Behavior was evaluated with morris water maze. RESULTS： CREB, pCREB, and Livin expression in the sevoflurane treatment group was significantly greater than the vascular cognitive impairment group （P 〈 0.01）. However, expression of CREB and pCREB was significantly less in the sevoflurane treatment and vascular cognitive impairment groups, compared with the sham operation group （P 〈 0.01）. Livin expression in the sevoflurane treatment and vascular cognitive impairment groups was significantly gre

关键词： vascular cognitive impairment sevoflurane cyclic adenosine monophosphate response element binding protein Livin

Spatiotemporal Mapping of Salt Marshes in the Intertidal Zone of China during 1985-2019

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Journal of Remote Sensing 2022年第1期2022卷 284-298页

作者： Guangwei Chen Runjie Jin Zhanjiang Ye Qi Li Jiali Gu Min Luo Yongming Luo George Christakos James morris Junyu He dan Li Hengwei Wang Li Song Qiuxuan Wang Jiaping Wu Ocean College Zhejiang UniversityZhoushan 316021China Ocean Academy Zhejiang UniversityZhoushan 316021China Institute of Soil Science Chinese Academy of SciencesNanjing 210008China Baruch Institute for Marine&Coastal Sciences University of South CarolinaSC 29208USA

This study mapped the areal extent,identified the species composition,and analyzed the changes of salt marshes in the intertidal zone of China during the period 1985–*** the aid of the cloud platform of the Google Earth Engine,we selected Landsat 5/8 and Sentinel-2 images and used the support vector machine classification method to extract salt marsh information for the years of 1985,1990,1995,2000,2005,2010,2015,and *** major species of salt marshes:Phragmites australis,Suaeda spp.,Spartina alterniflora,Scirpus mariqueter,Tamarix chinensis,Cyperus malaccensis,and Sesuvium portulacastrum were *** results showed that salt marshes are mainly distributed in Liaoning,Shandong,Jiangsu,Shanghai,and Zhejiang,with varying patterns of shrinking,expansion,or wavering in different *** distribution of salt marshes has declined considerably from 151,324 ha in 1985 to 115,397 ha in 2019,a drop of 23.7%.During the same period,the area of native species has dropped 95.4%from 77,741 ha to 3,563 ha for Suaeda *** 45.1%from 60,511 ha to 33,193 ha for ***;on the contrary,the area of exotic species,***,has exhibited a sharp rise from just 99 ha to 67,527 *** the past 35 years,the driving factors causing salt marsh changes are mainly land reclamation,variations in water and sand fluxes,and interspecific competition and succession of salt marsh *** results provide fundamental reference information and could form the scientific basis for formulating policies for the conservation and utilization of salt marsh resources in China.

关键词： driving Zone Mapping

小檗碱对慢性低灌注脑损伤小鼠神经保护作用的机制研究

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现代生物医学进展 2024年第10期24卷 1843-1847页

作者：常亚娟黄树明吴丹张博黑龙江中医药大学黑龙江哈尔滨150040 黑龙江中医药大学附属第二医院黑龙江哈尔滨150001

目的:探究小檗碱对慢性低灌注脑损伤小鼠神经保护作用的机制。方法:采用单侧颈总动脉永久性结扎(Unilateral Common Carotid Artery Occlusion UCCAO)方法建立慢性脑低灌注小鼠模型,随机分为5组(n=10),分别为假手术组、模型组、按小檗... 详细信息

目的:探究小檗碱对慢性低灌注脑损伤小鼠神经保护作用的机制。方法:采用单侧颈总动脉永久性结扎(Unilateral Common Carotid Artery Occlusion UCCAO)方法建立慢性脑低灌注小鼠模型,随机分为5组(n=10),分别为假手术组、模型组、按小檗碱剂量不同分为低剂量组、中剂量组和高剂量组,连续灌胃给药21天。采用走平横木实验和morris水迷宫评定小鼠精细运动能力和学习记忆能力。采用El ISA方法检测小鼠海马和皮质的炎症指标IL-1β、NLRP3和TNF-α。结果:与模型组比较,小檗碱中剂量和小檗碱高剂量组精细运动能力明显提高(P0.05)。与模型组比较,小檗碱中剂量和小檗碱高剂量组认知能力明显提高(P0.05)。与模型组比较,小檗碱中剂量和小檗碱高剂量组小鼠海马区和皮质区TNF-a、IL-1β、NLRP3的表达显著减少,小檗碱低剂量组IL-1β无显著性变化(P>0.05)。结论:小檗碱可提高慢性低灌注脑损伤小鼠的精细运动能力并对其认知功能障碍具有改善作用,同时减少神经炎症反应,具有神经保护作用。

关键词：小檗碱慢性脑低灌注神经炎症神经保护

Neonatal ketamine exposure-induced hippocampal neuroapoptosis in the developing brain impairs adult spatial learning ability

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Neural Regeneration Research 2020年第5期15卷 880-886页

作者： dan Lyu Ning Tang Andrew W. Womack Yong-Jin He Qing Lin Department of Pain Management Tianjin First Center HospitalTianjinChina Department of Psychology College of ScienceUniversity of Texas at ArlingtonArlingtonTXUSA Reproductive Medicine Center the 960^th Hospital of the PLA Joint Logistics Support ForceJinanShandong ProvinceChina

Ketamine exposure can lead to selective neuroapoptosis in the developing brain.p66ShcA,the cellular adapter protein expressed selectively in immature neurons,is a known pro-apoptotic molecule that triggers neuroapoptosis when ***-Dawley rats at postnatal day 7 were subcutaneously injected in the neck with ketamine 20 mg/kg,six times at 2-hour *** 0,1,3,and 6 hours after final injection,western blot assay was used to detect the expression of cleaved caspase-3,p66ShcA,and phosphorylated *** found that the expression of activated p66ShcA and caspase-3 increased after ketamine exposure and peaked at 3 *** same procedure was performed on a different group of *** the age of 4 weeks,spatial learning and memory abilities were tested with the morris water *** to find the hidden platform for these rats was longer than it was for control rats,although the residence time in the target quadrant was *** findings indicate that ketamine exposure resulted in p66ShcA being activated in the course of an apoptotic cascade during the neonatal *** may have contributed to the deficit in spatial learning and memory that persisted into *** experimental protocol was approved by the Institutional Animal Care and Use Committee at the University of Texas at Arlington,USA (approval No.A13.008) on January 22,2013.

关键词： caspase-3 developmental neuroapoptosis hippocampus ketamine morris water maze N-methyl-D-aspartate acid receptors p66ShcA spatial learning

基于PI3K/Akt/GSK-3β信号通路探讨七味白术散对糖尿病脑病大鼠认知功能障碍的影响

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中国实验方剂学杂志 2024年第3期30卷 10-17页

作者：高佳馨王建波薛亚楠孙杰王丹韩堃张云雨尹怡然冯晓帆辽宁中医药大学中西医结合学院中医脏象理论及应用教育部重点实验室沈阳110847

目的:观察七味白术散对糖尿病脑病(DE)大鼠模型的治疗作用,基于磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/糖原合成酶激酶-3β(GSK-3β)通路探究其可能存在的机制。方法:选取SPF级雄性Wistar大鼠48只,随机分为空白组8只、高脂饲料组40只... 详细信息

目的:观察七味白术散对糖尿病脑病(DE)大鼠模型的治疗作用,基于磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/糖原合成酶激酶-3β(GSK-3β)通路探究其可能存在的机制。方法:选取SPF级雄性Wistar大鼠48只,随机分为空白组8只、高脂饲料组40只。高脂饲料组大鼠喂养12周后,连续2 d腹腔注射35 mg·kg^(-1)的1%链脲佐菌素构建DE模型,空白组大鼠注射等量柠檬酸钠缓冲液。造模成功后,按血糖和体质量随机分为模型组、七味白术散低、中、高剂量组(3.15、6.3、12.6 g·kg^(-1))、联合西药组(二甲双胍+罗格列酮,0.21 g·kg^(-1)),每组各6只。给药组灌胃给予相应剂量药物,空白组和模型组灌胃等体积生理盐水,1次/d,连续给药6周。采用morris水迷宫检测DE大鼠空间记忆能力,酶联免疫吸附测定法(ELISA)检测空腹胰岛素(FINS)水平并计算胰岛素抵抗指数(HOMA-IR),苏木素-伊红(HE)染色观察大鼠海马组织病理变化,ELISA检测海马组织氧化应激指标,实时荧光定量聚合酶链式反应(Real-time PCR)检测海马组织PI3K、Akt、核转录因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、和白细胞介素-1β(IL-1β)mRNA表达,蛋白免疫印迹法(Western blot)检测大鼠海马中PI3K、Akt、磷酸化(p)-Akt、GSK-3β、p-GSK-3β蛋白表达情况。结果:与空白组比较,模型组大鼠FINS、HOMA-IR值均显著增高(P<0.01);找到平台原位置的路径显著增长,逃避潜伏期显著延长(P<0.01);海马组织神经细胞形态破坏;大鼠海马活性氧(ROS)、丙二醛(MDA)水平升高和超氧化物歧化酶(SOD)活性降低(P<0.05,P<0.01);PI3K、Akt mRNA表达水平显著降低(P<0.01),NF-κB、TNF-α和IL-1βmRNA表达水平升高(P<0.05,P<0.01);PI3K、p-Akt、p-GSK-3β蛋白表达水平下降(P<0.05,P<0.01),GSK-3β表达显著增加(P<0.01)。与模型组比较,七味白术散中剂量组和联合西药组FINS、HOMA-IR值均显著下降(P<0.01);大鼠找到平台原位置的路径显著缩短,逃避潜伏期缩短(P<0.01);大鼠海马组织结构逐渐恢复,神经细胞形态破坏程度明显改善;大鼠海马组织中ROS、MDA含量降低和SOD水平升高(P<0.01);PI3K、Akt mRNA表达水平显著升高(P<0.01),NF-κB、TNF-α和IL-1βmRNA表达水平降低(P<0.05,P<0.01);PI3K、p-Akt、p-GSK-3β蛋白表达升高,GSK-3β表达显著降低(P<0.01)。结论:七味白术散可以缓解DE大鼠的胰岛素抵抗,其可能是通过激活PI3K/Akt/GSK-3β信号通路来修复DE大鼠海马神经元损伤,改善DE大鼠学习认知能力。

关键词：糖尿病脑病七味白术散补脾益气法胰岛素抵抗磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶-3β(PI3K/Akt/GSK-3β)信号通路认知功能障碍

基于PI3K/AKT/GSK-3β信号通路探讨EA改善APP/PS1双转基因小鼠认知功能障碍的内在机制

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中国药理学通报 2024年第1期40卷 90-98页

作者：仲丽丽路鑫于颖赵秦妍张静刘彤慧倪雪妍车艳玲吴丹刘宏黑龙江中医药大学黑龙江哈尔滨150040 黑龙江中医药大学附属第一医院黑龙江哈尔滨150040 黑龙江中医药大学基础医学院黑龙江哈尔滨150040

目的探讨鞣花酸(ellagicacid,EA)对APP/PS1双转基因小鼠认知功能的影响,并基于磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶-3(PI3K/AKT/GSK-3β)信号通路探讨鞣花酸对双转基因小鼠海马氧化应激水平的调节机制。方法将32只SPF级6月龄APP/... 详细信息

目的探讨鞣花酸(ellagicacid,EA)对APP/PS1双转基因小鼠认知功能的影响,并基于磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶-3(PI3K/AKT/GSK-3β)信号通路探讨鞣花酸对双转基因小鼠海马氧化应激水平的调节机制。方法将32只SPF级6月龄APP/PS1双转基因小鼠随机分为4组,即APP/PS1组、APP/PS1+EA组、APP/PS1+LY294002组、APP/PS1+EA+LY294002组,每组8只,另外选取8只SPF级C57BL/6J野生型小鼠(Wildtype)作为空白对照组,即WT组。APP/PS1+EA组给予50mg·kg^(-1)·d^(-1)灌胃EA;APP/PS1+LY294002组予以1.5mg·kg^(-1)·d^(-1)腹腔注射PI3K抑制剂LY294002;APP/PS1+EA+LY294002组予以50mg·kg^(-1)·d^(-1)灌胃EA,同时按1.5mg·kg^(-1)·d^(-1)腹腔注射LY294002;WT组和APP/PS1组于相同时间点灌胃等体积10%二甲基亚砜(DMSO)。每日给药1次,连续给药60天。morris水迷宫检测小鼠学习和记忆能力,免疫组化、蛋白免疫印迹法检测PI3K、AKT、GSK-3β相关蛋白的表达,透射电镜观察小鼠海马组织超微结构变化。结果与WT组相比,其他四组的逃避潜伏期均增长(P<0.05),穿越平台次数明显减少(P<0.01);APP/PS1组、APP/PS1+LY294002组和APP/PS1+EA+LY294002组中的PI3K、AKT蛋白表达量显著降低(P<0.01),GSK-3β表达量显著升高(P<0.01);APP/PS1+EA组的PI3K表达量降低(P<0.05),AKT表达量显著降低(P<0.01),GSK-3β表达量升高(P<0.05);与WT组相比,APP/PS1组海马神经元细胞数目较少,线粒体结构破坏,大部分线粒体出现肿胀,线粒体的内膜和外模不完整,部分线粒体嵴消失,微管、微丝缠结,排列紊乱,而APP/PS1+EA组神经元细胞数较APP/PS1组增多,线粒体结构较清晰,可见清楚的线粒体嵴,线粒体轻度水肿。微管、微丝排列较整齐有序。结论鞣花酸改善AD模型小鼠的学习和记忆能力、减少海马神经元细胞损伤和凋亡,其作用机制可能是通过调节PI3K、AKT、GSK-3β等相关蛋白降低AD模型小鼠海马氧化应激水平。

关键词： APP/PS1双转基因小鼠阿尔茨海默病鞣花酸磷脂酰肌醇3-激酶蛋白激酶B 糖原合成酶激酶-3

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基于NF-κB/HIF-1α信号通路探讨慢性脑低灌注与神经炎症的相关性研究

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现代生物医学进展 2024年第11期24卷 2038-2042,2119页

作者：常亚娟黄树明湛清扬吴丹张博黑龙江中医药大学黑龙江哈尔滨150040 黑龙江中医药大学附属第二医院黑龙江哈尔滨150001

目的:探究NF-κB/HIF-1α信号通路在慢性脑低灌注引起神经炎症过程中的作用及机制。方法:采用单侧颈总动脉永久性结扎(Unilateral Common Carotid Artery Occlusion,UCCAO)方法建立慢性脑低灌注小鼠模型,随机分为3大组,每组50只,分别为... 详细信息

目的:探究NF-κB/HIF-1α信号通路在慢性脑低灌注引起神经炎症过程中的作用及机制。方法:采用单侧颈总动脉永久性结扎(Unilateral Common Carotid Artery Occlusion,UCCAO)方法建立慢性脑低灌注小鼠模型,随机分为3大组,每组50只,分别为正常对照组,假手术组和UCCAO组,每大组按1 d、3 d、7 d、14 d、21 d分为5小组。采用morris水迷宫评定学习记忆能力。采用RT-PCR法检测小鼠脑组织HIF-1α m RNA和NF-κB p65 m RNA相对表达水平。采用El ISA方法检测小鼠脑组织炎症指标TNF-α、IL-1β。结果:与假手术组比较,UCCAO组学习记忆明显下降(P<0.01)。与假手术组比较,UCCAO组小鼠脑组织HIF-1αm RNA和NF-κB p65 m RNA相对表达水平明显增加(P<0.01),HIF-1α m RNA和NF-κB p65 m RNA相对表达水平均于术后3 d开始明显增加,7 d出现高峰,且21 d后相对表达水平仍然明显增加(P<0.01)。与假手术组比较,UCCAO组小鼠脑组织匀浆液中TNF-α和IL-1β的含量明显增加(P<0.01)。结论:1.慢性持续性脑低灌注可导致小鼠出现进行性认知功能障碍。***后缺血缺氧既可以激活HIF-1α信号通路,又是NF-κB信号转导途径的刺激因子,这两条信号通路存在着诸多交叉,NF-κB活化对HIF-1α调控和炎症反应的形成正反馈。

关键词：慢性脑低灌注 NF-κB HIF-1α 神经炎症

同仁牛黄清心丸对血管性认知障碍大鼠学习记忆能力的影响及机制

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现代中西医结合杂志 2024年第15期33卷 2078-2082,2087页

作者：陈霞张思玉刘丹朱晓光李晋生北京同仁堂股份有限公司科学研究所北京100079

目的探讨同仁牛黄清心丸对血管性认知障碍大鼠学习记忆能力的影响及可能作用机制。方法取雄性Wistar大鼠90只,随机取11只作为假手术组,暴露但不结扎双侧颈总动脉;其余大鼠均采用结扎双侧颈总动脉方法建立血管性认知障碍模型。将造模组... 详细信息

目的探讨同仁牛黄清心丸对血管性认知障碍大鼠学习记忆能力的影响及可能作用机制。方法取雄性Wistar大鼠90只,随机取11只作为假手术组,暴露但不结扎双侧颈总动脉;其余大鼠均采用结扎双侧颈总动脉方法建立血管性认知障碍模型。将造模组大鼠随机分为模型组、尼莫地平组及同仁牛黄清心丸高、中、低剂量组,每组11只。尼莫地平组给予尼莫地平12 mg/kg灌胃,同仁牛黄清心丸高、中、低剂量组分别给予2.4 g/kg、1.2 g/kg、0.6 g/kg同仁牛黄清心丸灌胃,模型组和假手术组给予等体积纯化水灌胃,均1次/d。连续灌胃4周后,采用morris水迷宫实验测试大鼠学习记忆能力(记录测试象限内游泳路径长度和穿越原平台次数);处死大鼠,测定脑脊液中阿尔茨海默相关神经丝蛋白(AD7c-NTP)水平和脑组织中胆碱能指标[乙酰胆碱酯酶(AchE)]、氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)]、炎症指标[肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)]含量。结果与假手术组比较,模型组大鼠测试象限内游泳路径长度明显缩短(P<0.05),穿越原平台次数明显减少(P<0.05);与模型组比较,尼莫地平组和同仁牛黄清心丸高、低剂量组大鼠测试象限内游泳路径长度明显增加(P均<0.05),同仁牛黄清心丸高剂量组大鼠穿越原平台次数明显增多(P<0.05)。与假手术组比较,模型组大鼠脑脊液中AD7c-NTP含量和脑组织中AchE、MDA、IL-6、TNF-α含量均明显升高(P均<0.05),脑组织中SOD含量明显降低(P<0.05);与模型组比较,尼莫地平组和同仁牛黄清心丸高剂量组大鼠脑脊液中AD7c-NTP含量和脑组织中AchE含量、同仁牛黄清心丸低剂量组大鼠脑脊液中AD7c-NTP含量、同仁牛黄清心丸中剂量组大鼠脑组织中AchE含量及尼莫地平组和同仁牛黄清心丸各组大鼠脑组织中MDA、IL-6和TNF-α含量均明显降低(P均<0.05),尼莫地平组和同仁牛黄清心丸各组大鼠脑组织中SOD含量均明显增高(P均<0.05)。结论同仁牛黄清心丸能够提高血管性认知障碍大鼠的学习记忆能力,其可能通过改善胆碱能系统功能、抗氧化应激、减轻炎症反应而发挥脑保护作用。

关键词：同仁牛黄清心丸血管性认知障碍炎症因子氧化应激胆碱能系统

Tetrahydroxy Stilbene Glucoside Ameliorates Cognitive Impairments and Pathology in APP/PS1 Transgenic Mice

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Current Medical Science 2021年第2期41卷 279-286页

作者： dan GAO Chen CHEN Rui HUANG Cui-cui YANG Bei-bei MLAO Lin LI Lan ZHANG Department of Pharmacy Xuanwu Hospital of Capital Medical UniversitKey Laboratory for Neurodegenerative Diseases of Ministry of EducationBeiing Institute for Brain DisordersBeijing Engineering Research Center for Nerve System DrugsBeijing 100053China

Cognitive impairment is the main clinical manifestation of Alzheimer's disease(AD),and amyloid-β(AB)deposition and senile plaques are the characteristic neuropathological hallmarks in AD *** study aimed to explore the effect and mechanism of tetrahydroxy stilbene glucoside(TSG)on cognitive function in APP/PS 1 mice during long-term ***,we treated APP/PS1 model mice of AD with different doses of TSG(50 mg/kg and 100 mg/kg)for 5 to 17 months by gavage,and we further observed whether TSG could ameliorate the cognitive decline in APP/PS1 mice using behavioral tests,and investigated the possible mechanisms by immunohistochemistry and Western *** results showed that TSG treatment rescued the spatial and non-spatial learning and memory impairments of APP/PS1 mice at morris water maze test and novel object recognition ***,Aβ40/42 deposition in the cortex and hippocampus of APP/PS1 mice treated with TSG was significantly reduced compared to the wild type mice using the immunohistochemical ***,Western blotting showed that TSG primarily decreased the APP expression to avoid the Aβplaque deposition in the cortex and hippocampus of *** results reveal the beneficial effects of TSG in APP/PSI-AD mice,which may be associated with the reduction of Aβdeposits in the brain.

关键词： tetrahydroxy stilbene glucoside APP/PS1 transgenic mice cognitive impairments amyloid-βprotein