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Differential Expressing of Centromere Protein CenpG in Breast Cancer

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High Technology Letters 2002年第1期8卷 1-4页

作者：梁前进 Lu Xiangfeng Cheng Xiaolei Zhang Huanxiang he dacheng WANG Yongchao College of Life Sciences Beijing Normal University Beijing 100875 P.R.China Dongzhimen Hospital of Beijing University of Chinese Traditional Medicine Beijing 100700 P.R.China Tayuan Hospital of Beijing Red Cross Beijing 100083 China. Zhang Huanxiang

Using indirect immunofluorescence (IIF), an anti-centromere protein CenpG-serum was verified. Western blot of the protein extracts of 31 samples of breast cancer tissues and their normal (not cancerous ) tissues a little far away from them in the same individuals showed that, in the majority of the tests (71%), centromere protein CenpG over expressed in breast cancer tissues. And moreover, a kind of protein component whose molecular weight is 43 kd, and which can be recognized by anti-CenpG serum was found in two of the cancer samples. The results suggested that CenpG (together whith it, there may be other relative components),which has been found and named recently, may be related to cancer,and its differential expressing is probably related to malignant cell proliferation.

关键词： Breast cancer Centromere protein CenpG

Effects of antisense CENP-B on the proliferation of heLa (Tet-off) cells

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Chinese Science Bulletin 2002年第5期47卷 379-383页

作者： Qianjin Liang Qian Wang Haiyan Lin Song Luo Chunmei Wang dacheng he Yongchao Wang Beijing Normal Univ Coll Life Sci Beijing 100875 Peoples R China

In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected heLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of heLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with heLa (Tet-off), the G1 cell population of HaCb is increased (AG1=9%) while S fraction is decreased (AS=11%), but the G2/M phase is nearly unchanged (AG2/M=3%). In the meanwhile, the mi-totic index of HaCb declines greatly compared with that of heLa (Tet-off). Immunofluorescence showed that the

关键词： centromere proteins CENP-B heLa (Tet-off) cell.

REPORT ON CONSTRUCTION OF The ECO-FRIENDLY LHASA-SHIGATSE RAILWAY

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China's Tibet 2014年第6期25卷 4-9页

作者： he dacheng Jiang Xiaojun

Tibet is not only the origin of rivers,the source of ecology in South and Southeast Asia,but also an initiator and a conditioning zone for the Chinese and even the climate of the Eastern hemisphere,thus honored as the... 详细信息

关键词：生态友好日喀则拉萨铁路东南亚调节区引发剂东半球

A Long Type of TBCK Is a Novel Cytoplasmic and Mitotic Apparatus-Associated Protein Likely Suppressing Cell Proliferation

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Journal of Genetics and Genomics 2014年第2期41卷 69-72页

作者： Jin Wu Qianyi Li Yan Li Jing Lin Dong Yang Guixin Zhu Limin Wang dacheng he Guanting Lu Changqing Zeng Laboratory of Disease Genomics and Individualized Medicine Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory for Cell Proliferation and Regulation of the Ministry of Education Beijing Normal University

Autoantibodies from patients with various connective tissue diseases have been shown to be specific probes that can detect cellular structures, including centrosome, centromere/kineto- chore, spliceosome, Golgi comple... 详细信息

关键词： Cell TBC A Long Type of TBCK Is a Novel Cytoplasmic and Mitotic Apparatus-Associated Protein Likely Suppressing Cell Proliferation

miR-137 inhibits melanoma cell proliferation through downregulation of GLO1

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Science China(Life Sciences) 2018年第5期61卷 541-549页

作者： Na Lv Shuai Hao Chonglin Luo Alia Abukiwan Ying Hao Fei Gai Weiwei Huang Lingyun Huang Xueyuan Xiao Stefan B.Eichmüller dacheng he Key Laboratory of Cell Proliferation and Regulation of Ministry of Education Universities of the Confederated Institute for ProteomicsBeijing Normal University Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University Gene & Care health Technologies Co. Ltd. GMP & T Cell Therapy Unit German Cancer Research Center (DKFZ) College of Sports Northwest Normal University

Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma *** analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter *** RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 ***, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 ***-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.

关键词： melanoma miR-137 SiLAD GLO1 cell proliferation

heARTWARMING STORIES DURING CONSTRUCTION OF The LHASA-SHIGATSE RAILWAY

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China's Tibet 2014年第6期25卷 10-12页

作者： he dacheng Zhou Peng Jiang Xiaojun

The Lhasa-Shigatse Railway took 4 years to *** those 1,400 days,while solving the technical problems,the builders tried every means to help the Tibetan *** the saying goes,he who sows courtesy reaps *** genuine effort... 详细信息

关键词：故事日喀则西藏

CENP-K and CENP-H may form coiled-coils in the kinetochores

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Science China(Life Sciences) 2009年第4期52卷 352-359页

作者： QIU ShuLan, WANG JiaNing, YU Chuang & he dacheng The Key Laboratory for Cell Proliferation and Regulation Biology of the Ministry of Education, Beijing Normal University, Universities’ Confederated Institute of Proteomics, Beijing 100875, China The Key Laboratory for Cell Proliferation and Regulation Biology of the Ministry of Education Beijing Normal University Universities’ Confederated Institute of Proteomics Beijing 100875 China

Kinetochores are large proteinaceous structure on the surface of chromosomes’ primary constriction during mitosis. They link chromosomes to spindle microtubules and also regulate the spindle assem- bly checkpoint, which is crucial for correct chromosome segregation in all eukaryotes. The better known core networks of kinetochores include the KMN network (K, KNL1; M, Mis12 complex; N, Ndc80 complex)and CCAN (constitutive centromere-associated network). However, the detailed molecular mechanism of the kinetochore protein network remains unclear. This study demonstrates that CENP-H and CENP-K form quite stable subcomplex by TAP (tandem affinity purification) with heK 293 cells which express TAP-CENP-K, with the ratio of purified CENP-H and CENP-K being close to 1︰1 even with high salt. Bioinformatic analysis suggests that CENP-H and CENP-K are enriched with coiled-coil regions. This implies that CENP-H and CENP-K form heterodimeric coiled-coils. Furthermore, the func- tional regions which form the complex are respectively located on their N- and C-terminals, but the association between the C-terminals is more complex. It is possible that this is the first identified het- erodimeric coiled-coils within the inner kinetochore, which is directly involved in the attachment be- tween kinetochores and the spindle microtubules.

关键词： kinetochore CENP-H CENP-K heterodimeric coiled-coils

面向直流配电网尾端功率变换的组合式直流变压器研究

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电力自动化设备 2018年第7期38卷 43-50页

作者：刘闯何达成徐鑫哲东北电力大学电气工程学院吉林吉林132012 国网江苏省电力有限公司常州供电分公司江苏常州213000 国网北京电力公司北京100000

着力于未来直流配电网尾端功率变换应用研究,提出了一种新型高频隔离型组合式直流变压器(ADCT)概念,利用标准化模块来构建所期望的系统结构,并通过临近控制来协调模块功率,进而实现整体平衡。临近控制主要利用谐振变换器的电压箝位特性... 详细信息

着力于未来直流配电网尾端功率变换应用研究,提出了一种新型高频隔离型组合式直流变压器(ADCT)概念,利用标准化模块来构建所期望的系统结构,并通过临近控制来协调模块功率,进而实现整体平衡。临近控制主要利用谐振变换器的电压箝位特性实现电压平衡,同时利用电磁耦合电流平衡(MC-CB)单元保证模块间的电流平衡。基于标准化模块,将一种新型的IS-IPOP型串并联结构应用于ADCT中。最后,搭建的ADCT硬件实验系统也验证了所提方案的有效性和合理性。

关键词：直流配电网高频隔离临近控制谐振直流变换电磁耦合电流平衡

Structural and microwave absorption properties of CoFe_(2)O_(4)/residual carbon composites

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 221-232页

作者： Yuanchun Zhang Shengtao Gao Xingzhao Zhang dacheng Ma Chuanlei Zhu Jun he Joint National-Local Engineering Research Centre for Safe and Precise Coal Mining Anhui University of Science and TechnologyHuainan 232001China School of Chemical and Blasting Engineering Anhui University of Science and TechnologyHuainan 232001China

Electromagnetic interference,which necessitates the rapid advancement of substances with exceptional capabilities for bsorbing electromagnetic waves,is of urgent concern in contemporary *** this work,CoFe_(2)O_(4)/residual carbon from coal gasification fine slag(CFO/RC)composites were created using a novel hydrothermal *** mechanisms for microwave absorption,including conductive loss,natural resonance,interfacial dipole polarization,and magnetic flux loss,are involved in these ***,compared with pure residual carbon materials,this composite offers superior capabilities in microwave *** 7.76GHz,the CFO/RC-2 composite achieves an impressive minimum reflection loss(RL_(min))of-43.99 dB with a thickness of 2.44 ***,CFO/RC-3 demonstrates an effective absorption bandwidth(EAB)of up to 4.16 GHz,accompanied by a thickness of *** study revealed the remarkable capability of the composite to diminish electromagnetic waves,providing a new generation method for microwave absorbing materials of superior quality.

关键词： coal gasification slag residual carbon hydrothermal method microwave absorption CoFe_(2)O_(4)