miR-137 inhibits melanoma cell proliferation through downregulation of GLO1

作     者：Na Lv Shuai Hao Chonglin Luo Alia Abukiwan Ying Hao Fei Gai Weiwei Huang Lingyun Huang Xueyuan Xiao Stefan B.Eichmüller Dacheng He 

作者机构：Key Laboratory of Cell Proliferation and Regulation of Ministry of Education Universities of the Confederated Institute for ProteomicsBeijing Normal University Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University Gene & Care Health Technologies Co. Ltd. GMP & T Cell Therapy Unit German Cancer Research Center (DKFZ) College of Sports Northwest Normal University 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2018年第61卷第5期

页      面：541-549页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Special Funds for Major State Basic Research of China (2011CB915504) Coalition for National Science Funding (31171371 (2011–2015) to Dacheng He) he German Cancer Aid (Melanoma Research Network) to Dr. Stefan B. Eichmüller 

主　　题：房间 proteomics RT-PCR siRNA 病理学 指向 副产品 35S 

摘      要：Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma *** analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter *** RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 ***, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 ***-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.