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Multifocal structured illumination optoacoustic microscopy

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Light(Science & Applications) 2020年第1期9卷 568-576页

作者： Zhenyue Chen Ali Ozbek Johannes Rebling Quanyu Zhou Xose Luis Dean-Ben daniel razansky Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology University of Zurich and ETH ZurichZurichSwitzerland

Optoacoustic(OA)imaging has the capacity to effectively bridge the gap between macroscopic and microscopic realms in biological ***-resolution OA microscopy has so far been performed via point-by-point scanning with a focused laser beam,thus greatly restricting the achievable imaging speed and/or field of *** we introduce multifocal structured illumination OA microscopy(MSIOAM)that attains real-time 3D imaging *** this purpose,the excitation laser beam is shaped to a grid of focused spots at the tissue surface by means of a beamsplitting diffraction grating and a condenser and is then scanned with an acousto-optic deflector operating at kHz *** both phantom and in vivo mouse experiments,a 10mm wide volumetric field of view was imaged with 15 Hz frame rate at 28μm spatial *** proposed method is expected to greatly aid in biological investigations of dynamic functional,kinetic,and metabolic processes across multiple scales.

关键词： illumination beam excitation

Single-sweep volumetric optoacoustictomography of whole mice

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Photonics Research 2021年第6期9卷 899-908页

作者： SANDEEP KUMAR KALVA XOSE LUIS DEAN-BEN daniel razansky Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering Faculty of MedicineUniversity of ZurichZurichSwitzerland Institute for Biomedical Engineering Department of Information Technology and Electrical EngineeringETH ZurichZurichSwitzerland

Applicability of optoacoustic imaging in biology and medicine is determined by several key performance *** particular,an inherent trade-off exists between the acquired field-of-view(FOV)and temporal resolution of the measurements,which may hinder studies looking at rapid biodynamics at the whole-body ***,we report on a single-sweep volumetric optoacoustic tomography(sSVOT)system that attains whole body three-dimensional mouse scans within 1.8 s with better than 200μm spatial *** employs a spherical matrix array transducer in combination with multibeam illumination,the latter playing a critical role in maximizing the effective FOV and imaging speed *** system further takes advantage of the spatial response of the individual ultrasound detection elements to mitigate common image artifacts related to limited-view tomographic geometry,thus enabling rapid acquisitions without compromising image quality and *** compare performance metrics to the previously reported whole-body mouse imaging implementations and alternative image compounding and reconstruction *** is anticipated that sSVOT will open new venues for studying large-scale biodynamics,such as accumulation and clearance of molecular agents and drugs across multiple organs,circulation of cells,and functional responses to stimuli.

关键词： image latter illumination

Accounting for speed of sound variations in volumetric hand-held optoacoustic imaging

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Frontiers of Optoelectronics 2017年第3期10卷 280-286页

作者： X. Luis DEAN-BEN Ali OZBEK daniel razansky Institute of Biological and Medical Imaging (IBMI) Helmholtz Zentrum Miinchen Neuherberg Germany School of Medicine and School of Bioengineering Technical University of Munich Munich Germany

Hand-held implementations of recently intro- duced real-time volumetric tomography approaches repre- sent a promising path toward clinical translation of the optoacoustic technology. To this end, rapid acquisition of optoacoustic image data with spherical matrix arrays has attained exquisite visualizations of three-dimensional vascular morphology and function deep in human tissues. Nevertheless, significant reconstruction inaccuracies may arise from speed of sound （SOS） mismatches between the imaged tissue and the coupling medium used to propagate the generated optoacoustic responses toward the ultra- sound sensing elements. Herein, we analyze the effects of SoS variations in three-dimensional hand-held tomo- graphic acquisition geometries. An efficient graphics processing unit （GPU）-based reconstruction framework is further proposed to mitigate the SoS-related image quality degradation without compromising the high-frame-rate volumetric imaging performance of the method, essential for real-time visualization during hand-held scans.

关键词： speed of sound (SOS) graphics processingunit (GPU)

Transmission-reflection optoacoustic ultrasound(TROPUS)computed tomography of small animals

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Light(Science & Applications) 2019年第1期8卷 1051-1062页

作者： Elena Mercep Joaquin LHerraiz Xose Luis Dean-Ben daniel razansky Faculty of Medicine Technical University of MunichMunichGermany iThera Medical GmbH MunichGermany Nuclear Physics Group and UPARCOS Complutense University of MadridCEI MoncloaMadridSpain Health Research Institute of Hospital Clinico San Carlos(IdISSC) MadridSpain Institute for Biological and Medical Imaging(IBMI) Helmholtz Center MunichNeuherbergGermany Faculty of Medicine and Institute of Pharmacology and Toxicology University of ZurichZurichSwitzerland Institute for Biomedical Engineering and Department of Information Technology and Electrical Engineering ETH ZurichZurichSwitzerland

Rapid progress in the development of multispectral optoacoustic tomography techniques has enabled unprecedented insights into biological dynamics and molecular processes in vivo and noninvasively at penetration and spatiotemporal scales not covered by modern optical microscopy *** imaging provides highly complementary information on elastic and functional tissue properties and further aids in enhancing optoacoustic image *** devised the first hybrid transmission–reflection optoacoustic ultrasound(TROPUS)small animal imaging platform that combines optoacoustic tomography with both reflection-and transmission-mode ultrasound computed *** system features full-view cross-sectional tomographic imaging geometry for concomitant noninvasive mapping of the absorbed optical energy,acoustic reflectivity,speed of sound,and acoustic attenuation in whole live mice with submillimeter resolution and unrivaled image ***-processing unit(GPU)-based algorithms employing spatial compounding and bent-ray-tracing iterative reconstruction were further developed to attain real-time rendering of ultrasound tomography images in the full-ring acquisition *** vivo mouse imaging experiments revealed fine details on the organ parenchyma,vascularization,tissue reflectivity,density,and *** further used the speed of sound maps retrieved by the transmission ultrasound tomography to improve optoacoustic reconstructions via two-compartment *** newly developed synergistic multimodal combination offers unmatched capabilities for imaging multiple tissue properties and biomarkers with high resolution,penetration,and contrast.

关键词： reflectivity rendering details

Localization optoacoustic tomography

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Light(Science & Applications) 2018年第1期7卷 1058-1062页

作者： X Luís Dean-Ben daniel razansky Institute for Biological and Medical Imaging Helmholtz Center MunichNeuherberg 85764Germany School of Medicine and School of Bioengineering Technical University of MunichMunich 81675Germany

Localization-based imaging has revolutionized fluorescence optical microscopy and has also enabled unprecedented ultrasound images of microvascular structures in deep ***,we introduce a new concept of localization optoacoustic tomography(LOT)that employs rapid sequential acquisition of three-dimensional optoacoustic images from flowing absorbing *** show that the new method enables breaking through the spatial resolution barrier of acoustic diffraction while further enhancing the visibility of structures under limited-view tomographic *** the intrinsic sensitivity of optoacoustics to multiple hemodynamic and oxygenation parameters,LOT may enable a new level of performance in studying functional and anatomical alterations of microcirculation.

关键词： acoustic diffraction limited-view effects localization optoacoustic imaging photoacoustic imaging super-resolution

Non-invasive determination of murine placental and foetal functional parameters with multispectral optoacoustic tomography

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Light(Science & Applications) 2019年第1期8卷 553-562页

作者： Kausik Basak Xose Luis Dean-Ben Sven Gottschalk Michael Reiss daniel razansky Faculty of Medicine Technical University MunichMunichGermany Institute for Biological and Medical Imaging Helmholtz Center MunichNeuherbergGermany Faculty of Medicine and Institute of Pharmacology and Toxicology University of ZurichZurichSwitzerland Institute for Biomedical Engineering and Department of Information Technology and Electrical Engineering ETH ZurichZurichSwitzerland Institute of Advanced Studies and Research JIS UniversityKolkataWest BengalIndia

Despite the importance of placental function in embryonic development,it remains poorly understood and challenging to characterize,primarily due to the lack of non-invasive imaging tools capable of monitoring placental and foetal oxygenation and perfusion parameters during *** developed an optoacoustic tomography approach for real-time imaging through entire ~4 cm cross-sections of pregnant *** changes in both maternal and embryo regions were studied at different gestation days when subjected to an oxygen breathing challenge and perfusion with indocyanine *** phenotyping of the cross-sectional scans highlighted different internal organs,whereas multi-wavelength acquisitions enabled non-invasive label-free spectroscopic assessment of blood-oxygenation parameters in foeto-placental regions,rendering a strong correlation with the amount of oxygen ***,the placental function in protecting the embryo from extrinsically administered agents was *** proposed methodology may potentially further serve as a probing mechanism to appraise embryo development during pregnancy in the clinical setting.

关键词： rendering placental sectional

Hybrid magnetic resonance and optoacoustic tomography(MROT) for preclinical neuroimaging

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Light(Science & Applications) 2022年第12期11卷 2961-2971页

作者： Zhenyue Chen Irmak Gezginer Mark-Aurel Augath Wuwei Ren Yu-Hang Liu Ruiqing Ni Xose Luis Dean-Ben daniel razansky Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology Faculty of MedicineUniversity of ZurichZurichSwitzerland Institute for Biomedical Engineering Department of Information Technology and Electrical EngineeringETH ZurichZurichSwitzerland Zurich Neuroscience Center(ZNZ) ZurichSwitzerland

Multi-modal imaging is essential for advancing our understanding of brain function and unraveling pathophysiological processes underlying neurological and psychiatric *** resonance(MR)and optoacoustic(OA)imaging have been shown to provide highly complementary contrasts and capabilities for preclinical *** integration between these modalities can thus offer unprecedented capabilities for studying the rodent brain in *** report on a hybrid magnetic resonance and optoacoustic tomography(MROT)system for concurrent noninvasive structural and functional imaging of the mouse *** OA tomography was designed as an insert into a high-field MR scanner by integrating a customized MR-compatible spherical transducer array,an illumination module,and a dedicated radiofrequency coil.A tailored data processing pipeline has been developed to mitigate signal crosstalk and accurately register image volumes acquired with T1-weighted,angiography,and blood oxygenation level-dependent(BOLD)sequences onto the corresponding vascular and oxygenation data recorded with the OA *** demonstrate the concurrent acquisition of dual-mode anatomical and angiographic brain images with the scanner,as well as real-time functional readings of multiple hemodynamic parameters from animals subjected to oxygenation *** approach combines the functional and molecular imaging advantages of OA with the superb soft-tissue contrast of MR,further providing an excellent platform for cross-validation of functional readings by the two modalities.

关键词： scanner resonance illumination

Spiral volumetric optoacoustic tomography visualizes multi-scale dynamics in mice

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Light(Science & Applications) 2016年第1期5卷 104-111页

作者： X Luís Deán-Ben Thomas F Fehm Steven J Ford Sven Gottschalk daniel razansky Institute for Biological and Medical Imaging(IBMI) Helmholtz Center Munich85764 NeuherbergGermany School of Medicine and School of Bioengineering Technical University of Munich81675 MunichGermany

Imaging dynamics at different temporal and spatial scales is essential for understanding the biological complexity of living organisms,disease state and *** imaging has been shown to offer exclusive applicability across multiple scales with excellent optical contrast and high resolution in deep-tissue ***,efficient visualization of multi-scale dynamics remained difficult with state-of-the-art systems due to inefficient trade-offs between image acquisition time and effective field of ***,we introduce the spiral volumetric optoacoustic tomography technique that provides spectrally enriched highresolution contrast across multiple spatiotemporal *** vivo experiments in mice demonstrate a wide range of dynamic imaging capabilities,from three-dimensional high-frame-rate visualization of moving organs and contrast agent kinetics in selected areas to whole-body longitudinal studies with unprecedented image *** newly introduced paradigm shift in imaging of multi-scale dynamics adds to the multifarious advantages provided by the optoacoustic technology for structural,functional and molecular imaging.

关键词： multi-scale dynamics multi-spectral imaging optoacoustic tomography real-time imaging whole-body imaging

Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators

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Light(Science & Applications) 2016年第1期5卷 288-294页

作者： X Luís Deán-Ben Gali Sela Antonella Lauri Moritz Kneipp Vasilis Ntziachristos Gil G Westmeyer Shy Shoham daniel razansky Institute for Biological and Medical Imaging(IBMI) Helmholtz Center MunichNeuherbergGermany Institute of Developmental Genetics Helmholtz Center MunichNeuherbergGermany Department of Medicine Technical University of MunichMunichGermany Department of Biomedical Engineering Technion-Israel Institute of TechnologyHaifaIsrael

Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of *** developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering *** can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm^(3) with respective spatial resolution of 35–200μ*** performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate,for the first time,the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering *** newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics;in combination with optoacoustics'well-established capacity for resolving vascular hemodynamics,it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.

关键词： functional neuro-imaging genetically encoded calcium indicators high spatiotemporal resolution large-scale brain activity optoacoustic tomography photoacoustics real-time imaging