Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators

作     者：X Luís Deán-Ben Gali Sela Antonella Lauri Moritz Kneipp Vasilis Ntziachristos Gil G Westmeyer Shy Shoham Daniel Razansky 

作者机构：Institute for Biological and Medical Imaging(IBMI)Helmholtz Center MunichNeuherbergGermany Institute of Developmental GeneticsHelmholtz Center MunichNeuherbergGermany Department of MedicineTechnical University of MunichMunichGermany Department of Biomedical EngineeringTechnion-Israel Institute of TechnologyHaifaIsrael 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2016年第5卷第1期

页      面：288-294页

核心收录：

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

基　　金：support from the European Research Council ERC-2010-StG-260991(DR)and ERC-2012-StG_20111109(AL and GGW) the National Institute of Health R21-EY026382-01(DR and SS) the German-Israeli Foundation(GIF)for Scientific Research and Development 1142-46.10/2011(DR and SS) the Helmholtz Association of German Research Centers and the Technische Universität München(DR and GGW) 

主　　题：functional neuro-imaging genetically encoded calcium indicators high spatiotemporal resolution large-scale brain activity optoacoustic tomography photoacoustics real-time imaging 

摘      要：Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of *** developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering *** can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm^(3) with respective spatial resolution of 35–200μ*** performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate,for the first time,the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering *** newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics;in combination with optoacoustics well-established capacity for resolving vascular hemodynamics,it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.